• Journal of Korean Medicine Rehabilitation
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• v.28 no.3
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• pp.13-25
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• 2018

Objectives We investigated anti-obesity effects of essential oils extracted from Cnidii Rhizoma (CR) in immature adipocytes to magnify it's clinical therapeutic usage. Methods Essential oil of CR was extracted with ethyl acetate or petroleum ether and through steam distillation, respectively. Oil red-O staining for monitoring its inhibition effect on adipogenesis and differentiation in murine 3T3-L1 adipocytes and 3-(4,5-methylthiazol-2-yl)-2,5-diphenyletetra zolium bromide (MTT) assay for cell safety were done. Also phospho-adenosine monophosphate (AMP)-activted protein kinase (P-AMPK), AMP-activated protein kinase, phospho-acetyl-CoA carboxylase (P-ACC), acetyl-CoA carboxylase, peroxisome proliferator-activated receptor-${\alpha}$ (PPAR-${\alpha}$), peroxisome proliferator-activated receptor-${\gamma}$ (PPAR-${\gamma}$) and CCAAT/enhancer binding protein ${\alpha}$ (C/EBP-${\alpha}$) expressions as obesity-related factors were measured by western blot analysis. Results Protein expressions of P-AMPK, P-ACC and PPAR-${\alpha}$ were increased in essential oils-treated adipocytes compared to those of control group, respectively. Furthermore, protein expressions of PPAR-${\gamma}$ and C/EBP-${\alpha}$ were decreased in essential oils-treated adipocytes compared to those of control group, respectively. Conclusions These results demonstrate that essential oils of CR inhibit adipogenesis and differentiation. Also they promote the oxidation of fatty acids in adipocytes. Thus, results suggest that essential oils of CR could be used as a valuable material for anti-obesity therapeutics via control of lipid metabolism.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.10 no.3
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• pp.147-159
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• 2015

One of the most important factors for the consumers to select a coffee shop is a visual factor. The analysis results of interior pale face image of the coffee house doesn't show significant differences on all the categories. It is recognized that the items affected by symbolized color are supplies and interior design. If a respondent choose a coffee house by the symbolized color, 'Starbucks' is the most memorable or want to visit place, and only one category, pocket money shows significant difference in choosing coffee houses. The investigation results say that the most memorable or want to visit coffee house by the visual icon color are 'Starbucks', 'Angelinus' and 'Caffebene', and that 'Ediya's icon color marks the characteristics of the shop very well, and 'Hollys' has the most characterful color. Resultingly, visual elements and icon colors of the shop seriously affect consumer's choice in visiting a coffee house. This study is to understand the influence of visual elements in choosing a coffee house for general customers, and it is considered that the importance of visual elements and color marketing must be recognized and developed.

• Korean Journal of Environmental Biology
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• v.31 no.2
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• pp.121-153
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• 2013

We investigated the flora of Mudeung-san (Mt.) located in Gwangju-gwangyeoksi, Hwasun-gun and Damyang-gun and discussed the vascular plants found there. 22 days separate fild trips were completed from March of 2009 to November 2011. As a result, we identified the distribution of 816 taxa in Mudeung-san (Mt.), comprising 123 families, 441 genera, 668 species, 5 subspecies, 121 varieties and 18 forms. There are a total 111 taxa of floristic regional indicator plants. The floristic regional indicator plants are as follows; nine taxa of level V such as Gastrodia elata Blume, six taxa of level IV such as Hylomecon hylomeconoides T. Lee, 21 taxa of level III such as Stewartia korena Nakai, 18 taxa of level II, and 66 taxa of level I. In addition, two critically endangered species (CR), Galeola septentrionalis Rchb.f. and Paeonia obovata Maxim., two endangered species (EN), Dryopteris cycadina (Franch. & Sav.) C.Chr. and Jeffersonia dubia Benth., and eight vulnerable species (VU), such as Lilium callosum Siebold & Zucc, were found in the investigated area. Also, 14 endermic plants of Korea, such as Salix koriyanagi Kimura and Aconitium chiisanense Nakai, were found.

• Food Science of Animal Resources
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• v.33 no.5
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• pp.610-616
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• 2013

Loop-mediated isothermal amplification (LAMP) is an emerging detection technology for the amplification of DNA under isothermal conditions. The aim of this study was to develop a rapid and reliable LAMP technique for the detection of Cronobacter sakazakii in milk powder. In order to enhance the sensitivity and specificity, LAMP primers targeting outer membrane protein A (ompA) gene of C. sakazakii were designed using Explorer V4 software. Thirty seven C. sakazakii strains and 13 pathogenic microorganisms were used for comparative detection of C. sakazakii using polymerase chain reaction (PCR), real-time PCR, and LAMP. LAMP developed in this study could specifically detect C. sakazakii strains without cross-reactivity with other foodborne pathogens. LAMP products amplified from ompA gene of C. sakazakii were digested with with HhaI and NruI enzyme. The specificity of LAMP was confirmed by restriction fragment length polymorphism (RFLP) analysis. LAMP could detect C. sakazakii within 1 h without bacterial culture and its detection limit was as low as 1 CFU/mL C. sakazakii in milk. In the comparison of the sensitivity, LAMP showed 10,000- and 100-times higher detection limit than PCR or real-time PCR, respectively. Therefore, this study can conclude that LAMP is a rapid and reliable detection technique for C. sakazakii contaminated in powdered milk.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2002.04a
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• pp.69-75
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• 2002

Sterols play two major roles in plants: a bulk component in biological membranes and precursors of plant steroid hormones. Physiological effects of plant steroids, brassinosteroids (BRs), include cell elongation, cell division, stress tolerance, and senescence acceleration. Arabidopsis mutants that carry genetic defects in BR biosynthesis or its signaling display characteristic phenotypes, such as short robust inflorescences, dark-green round leaves, and sterility. Currently there are more than 100 dwarf mutants representing 7 genetic loci in Arabidopsis. Mutants of 6 loci, dwf1/dim1/cbb1, cpd/dwf3, dwf4, dwf5, det2/dwf6, dwf7 are rescued by exogenous application of BRs, whereas bri1/dwf2 shares phenotypes with the above 6 loci but are resistant to BRs. These suggest that the 6 loci are defective in BR biosynthesis, and the one locus is in BR signaling. Biochemical analyses, such as intermediate feeding tests, examining the levels of endogenous BR, and molecular cloning of the genes revealed that dwf7, dwf5, and dwf1 are defective in the three consecutive steps of sterol biosynthesis, from episterol to campesterol via 5-dehydroepisterol. Similarly, det2/dwf6, dwf4, and cpd/dwf3 were shown to be blocked in $D^4$ reduction, 22a-hydroxylation, and 23 a-hydroxylation, respectively. A signaling mutant bri1/dwf2 carries mutations in a Leucine-rich repeat receptor kinase. Interestingly, the bri1 mutant was shown to accumulate significant amount of BRs, suggesting that signaling and biosynthesis are dynamically coupled in Arabidopsis. Thus It is likely that transgenic plants over-expressing the rate-limiting step enzyme DWF4 as well as blocking its use by BRI1 could dramatically increase the biosynthetic yield of BRs. When applied industrially, BRs will boost new sector of plant biotechnology because of its potential use as a precursor of human steroid hormones, a novel lead compound for cholesterol-lowering effects, and a various application in plant protection.

• Korean Security Journal
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• no.50
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• pp.35-57
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• 2017

Security Act has been partially revised many times since it was revised to "Security Service Act". Main contents of such revision consist of the addition of security work such as protection or special security, responsibility enforcement of security company or security guard and systematic management of security service based on security work of previous security service act. But, it needs to be checked out that the fundamental matter about the concept of 'security' is directly related as double-edged sword in such flow of legal revision. That is because security service satisfies the multiple needs for security in the modern risky society and is based on the concept of active management whose goal is to forster and develop the function of actual security service comparing that current "Security Service Act" regulates the formal security service whose goal is permission of security service and systematic management based on article 2 as previous facilities and manned security that is guard duty-centered security service in another respect. So, this study pointed out the limit of interpreting security and security service in "Security Services Industry Act" in respect of providing private security service and drew the conclusion that the legislation and efforts are required for 'security for citizen' by reinterpreting the legislation and revision of private security service-related law as the normal regulation of "Security Services Industry Act" and the special law of "Private Security Services Industry Act".

• Proceedings of the Korea Water Resources Association Conference
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• 2006.05a
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• pp.483-487
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• 2006

본 연구는 비점오염원에 의한 수질오염현상을 억제하기 위한 수변지역의 관리기법 중 하나인 식생여과대를 효과적으로 설치하기 위하여 식생에 의한 오염물질의 저감능력을 파악하고 효율적 설치방안을 모색하기 위하여 수행되었다. 연구결과 T-N과 T-P의 깊이별 오염물질 저감효과는 나대지에서 표면유출이 각각 17.6%, -23.9%, 단면유출이 각각 51.7%, 91.0%, 하부유출이 각각 38.4%, 89.3%인 것으로 나타나 표면층을 통한 유출보다는 토양층에 침투될수록 오염물질의 저감효과가 높아지는 것으로 나타났다. 잔디로 표면층을 식재한 실험에서는 표면, 단면, 하부유출에서의 T-N이 각각 16.0%, 57.1%, 38.4%의 오염물질 저감효과를 보였고, T-P의 저감효과는 각각 -9.7%, 83.6%, 88.8%인 것으로 조사되었다. 또한 돌미나리로 표면층을 식재한 실험에서는 표면, 단면, 하부유출에서의 T-N 처리효율이 각각 -2.6%, 62.1%, 71.2%로 나타났으며, T-P는 각각 -7.2%, 94.5%, 84.5%인 것으로 조사되었다. 결론적으로 식생을 한 경우 전체적으로 오염물질의 저감효율이 비교적 안정되게 유지되는 것으로 나타났으나, T-P의 표면층을 제외하면 전체적인 저감효과에 크게 영향을 미치지 않은 것으로 판단된다. 그러나 일반적으로 알려져 있는 식생에 따른 오염물 저감효과에 대한 순기능 즉 토양입자의 침식방지, 토양용액의 저류작용 및 공극확대에 따른 침투능력 증대, 토성의 개량 등과 같은 기능을 무시할 수 없으므로 오염저감효과를 극대화하기 위해서는 처리대상이 되는 하천유역의 자생적 식생을 보다 효과적으로 활용할 수 있는 방안마련이 비용적 측면이나 생태 보존적 측면에서 유리할 것으로 판단된다.one)을 설치하는 대책이 필요하다. 저수지 관리를 효과적으로 수행하기 위해서는 저수지 내부의 탁도 거동을 정확히 예측할 수 있어야 한다. 따라서 추후 동수역학 및 열역학에 기초한 3차원 수치모형 연구와 성층흐름에 정밀한 밀도류 실험연구 및 이에 대한 적용이 필요할 것으로 판단된다.함으로써 정보의 질적보장과 정보전환의 표준화방안을 제시하는 정보분석시스템이다.이용, 수자원의 지속적 확보기술의 특성에 따른 4개의 평가기준과 26개의 평가속성으로 이루어진 2단계 기술가치평가 모형을 구축하였으며 2개의 개별기술에 대한 시범적용을 실행하였다.하는 것으로 추정되었다.면으로의 월류량을 산정하고 유입된 지표유량에 대해서 배수시스템에서의 흐름해석을 수행하였다. 그리고, 침수해석을 위해서는 2차원 침수해석을 위한 DEM기반 침수해석모형을 개발하였고, 건물의 영향을 고려할 수 있도록 구성하였다. 본 연구결과 지표류 유출 해석의 물리적 특성을 잘 반영하며, 도시지역의 복잡한 배수시스템 해석모형과 지표범람 모형을 통합한 모형 개발로 인해 더욱 정교한 도시지역에서의 홍수 범람 해석을 실시할 수 있을 것으로 판단된다. 본 모형의 개발로 침수상황의 시간별 진행과정을 분석함으로써 도시홍수에 대한 침수위험 지점 파악 및 주민대피지도 구축 등에 활용될 수 있을 것으로 판단된다. 있을 것으로 판단되었다.4일간의 기상변화가 자발성 기흉 발생에 영향을 미친다고 추론할 수 있었다. 향후 본 연구에서 추론된 기상변화와 기흉 발생과의 인과관계를 확인하고 좀 더 구체화하기 위한 연구가 필요할 것이다.게 이루어질 수 있을 것으로 기대된다.는 초과수익률이 상승하지만, 이후로는 감소하므로, 반전거래전략을 활용하는 경우 주식투자기간은 24개월이하의 중단기가 적합함을 발견하였

• Korean Journal of Environment and Ecology
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• v.24 no.5
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• pp.503-518
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• 2010

The Wando Jeongdo-ri Windbreak Forest is located in region of Wando, where located between sea and land facing with the southward. Due to the strong winds from the southeast in the summer season, it is very important sites as an ecological succession areas that protect cultivated land and the fishing village. Flora of Wando Jeongdo-ri Windbreak Forest is recorded as 384 taxa with 93 families 250 genera 355 species 42 varieties and 7 forms. The Jeongdo-ri Windbreak Forest belongs to South coast province, and appeared Cymbidium nipponicum as Endangered Plant Species II. Rare Plants are recorded as 6 taxa; C. nipponicum, Arisaema heterophyllum, Lilium distichum, Koelreuteria paniculata, Monotropa uniflora and Ardisia macrocarpa. The Protection species which is designated by the CITES are 2 taxa; Cymbidium goeringii and C. nipponicum. Protection species of IUCN is 1 taxa; Cinnamomum japonicum. Endemic plant are 7 taxa; Poa annua, Polygonum lasianthum var. coreanum, Chloranthus fortunei, Carpinus coreana and Lonicera subhispida. The naturalized plant is recorded as 32 taxa with 11 families 23 genera 27 species 5 varieties. The Naturalization Index was 8.33%, and the Urbanization Index was 11.81%.

• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.4
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• pp.405-411
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• 1993

Several studies have shown that phytol isolated from perilla leaf and small water dropwort (Oenanthe javanica (BL) D.C., wild type) extract reveal antirumor activities. In the present study we investigated the effect of phytol and the small water dropwort extract on the T subset in the sarcoma 180-transplanted mice in order to elucidate the immunological mechanism of antiturmor activity of these substances. The results obtained from the experiment were as follows : When phytol was injected into the sarcoma-180 transplanted mice (control), the levels of T cell and T subset by inoculation of the tumor cells were enhanced, but that of T cells in case of small water dropwort extract was similar to the control. Asialo GM1$^{＋}$ cells were increased when phytol or small water dropwort extract with injected into tumor-transplanted mice. In normal mice the number of asialo GM1$^{＋}$ cells increased with phytol injection and decreased with small water dropwort extract injection. L3T4$^{＋}$/Lyt-2$^{＋}$cell ratios were decreased when phytol was injected into tumor-transplanted mire, but increased in case of small water dropwort extract injection. In normal mice the ratios showed large decreases with phytol or small water dropwort extract injection. These results indicate that phytol or small water dropwort extract ran activate the proliferation of natural killer cells that are effector cells in tumor-bearing mice.

• Journal of Korea Port Economic Association
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• v.24 no.2
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• pp.131-154
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• 2008

The purpose of this paper is to verify the financial validity of dredging the port of Busan with the change of global port logistics circumstances. In order to do so, NPV, IRR, B/C ratio, DPP techniques are adopted. The whole dredging at Northern port area is supposed to be negative at the depth of 16m water. Based upon its financial validity, however, the priority is Shinsundae (option 1) followed by Shinsundae (option 1) and Gamman (option 1), Shinsundae (option 1) and Gamman (option 2), Shinsundae (option 2) and Gamman (option 2), Shinsundae (option 2) and Gamman (option 2) and ShinGamman in sequence. In addition to this, Busan New port is considered to be the positive financial validity at the dredging of 16m water depth. This is because the ultra containerships of over 8,000TEU are trying to call at the port and Hanjin shipping and Hyundai Merchant shipping companies which are now the most significant customers in Northern port are scheduled to divert it.