• Horticultural Science & Technology
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• v.19 no.3
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• pp.373-377
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• 2001

This study was conducted to find optimum pollination methods for overcoming pre-fertilization incompatibility in interspecific crosses between L. longiflorum 'Gelria' (LG) and L. cernuum (LC). In cross combination of $LC{\times}LG$, the survival rate of the ovaries was higher in the cut style pollinations than in normal stigmatic pollination. Among the various cut style pollination methods, pollination after cutting the styles 2 3mm length above the ovary and top of the ovary were the most effective ones. No ovaries and ovules survived in cross pollination of $LC{\times}LG$. In self-pollination of LG and cross pollination of $LC{\times}LG$, pollens germinated on the stigma by 12 hours and reached to the ovules by 90 hours after pollination. However, in the cross pollination of $LC{\times}LG$, pollen tubes elongated very slowly until 48 hours after pollination and a lot of deformed tubes were observed in the styler canal having swelled tube tip and irregular heavy callose deposition. The pollination time after anthesis also affected on the survival rate of ovaries and ovules showing the best result when pollinated on three days after anthesis. Stigma exudate promoted slightly the enlargement of ovaries, but the survival rate of ovaries and ovules was not increased.

• Journal of Plant Biotechnology
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• v.34 no.1
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• pp.25-29
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• 2007

Plant regeneration system from zygotic embryos (2n=24) of Lilium lancifolium Thunb. via bulblet formation was estabished. Zygotic embryos of Lilium lancifolium formed bulblets and somatic embryos simultaneously when they cultured on MS medium supplemented with low concentration of 2,4-D. The highest frequency of bulblet and somatic embryo formation from zygotic embryos of Lilium lancifolium was 66.7% and 56.7%, respectively. The frequency of bulblet and somatic embryo formation was decreased when they cultured on MS medium over than 1 mg/L of 2,4-D. To regenerate whole plants, somatic embryos formed on zygotic embryos were transferred to MS basal medium. However somatic embryos did not fully converted into plantlets. Further incubation in the light, elongated somatic embryos formed numerous bulblets at the base of somatic embryos. Upon transfer to MS basal medium, bulblets were successfully converted into plantlets after further 4 weeks of culture in the light. After acclimatization, plantets from bulblets were transferred to soil and grown to normal plants in growth chamber (approximately $30\;{\mu}mol\;m^{-2}s^{-1}$, 16/8h photo period, $25^{\circ}C$) The chromosome analysis revealed that plants regenerated from zygotic embryos showed 2n=24. These results indicate that chromosome stability of source tissue is maintained during plant regeneration via bulblet formation.

• Journal of Plant Biotechnology
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• v.40 no.4
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• pp.224-230
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• 2013

Lily is an important cut-flower in Korea and world as well due to it's a variety of flower colors and various sizes of flowers. To develop elite lily cultivars, conventional breeding techniques have been used so far. However, an introduction of tissue culture system in mass propagation of bulbs and regeneration of shoots with a high efficiency is prerequisite at this moment. Especially, growth of bulbs and shoots as well as reduction of browning is critical factor to proliferate bulbs with shoots of superior lines or cultivar in lily. For this purpose, we tried to test whether ascorbic acid, citric acid and silver nitrate in medium to facilitate growth of bulbs and shoots as well as reduction of browning of bulb scales in lily. As a result, ascorbic acid and silver nitrate showed no significant differences compared to control plants in the growth of bulbs and shoots. When bulb scales were treated with $150mg{\cdot}l^{-1}$ of citric acid, formations of shoots and bulbs showed best result. While, bulb scale treated with $100mg{\cdot}l^{-1}$ of citric acid showed the growth of shoot and root as well as increasing of fresh weight compared to other treatments. Regarding the reduction of browning, $150mg{\cdot}l^{-1}$ of ascorbic acid showed the best result with the less than 2%. Although more experiments with several commercial varieties are needed in the future to establish mass propagation of bulbs of lily, results obtained in this study are supposed to provide the basic knowledge and contribution in tissue culture system of lily.

• Research in Plant Disease
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• v.19 no.1
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• pp.7-11
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• 2013

Forty eight isolates of Botrytis elliptica and 23 isolates of B. cinerea from several locations in Korea were tested for resistance to fungicides used in the farmer's fields. Isolation frequency of B. elliptica having $EC_{50}$ (effective concentration of 50%) value $500-1000{\mu}g/ml$ to benomyl and mancozeb appeared highly, suggesting that the two fungicides are not effective in controlling leaf blight of lily in the field. The isolates were tested for resistance to fungicides procymidone and iprodione which were most commonly used in the farmer's fields. The rates of $EC_{50}$ value $5-50{\mu}g/ml$ to procymidome and iprodione were 93.7% and 100%, respectively, and those of $0-0.1{\mu}g/ml$ to diethofencarb+carbendazim and fludioxonil were 98.0% and 93.8%, respectively. In the rain-protected cultivation, control of leaf blight of lily was the most effective when iprodine, diethofencarb+carbendazim, and fludioxonil were sprayed alternately four times during the growing season.

• Economic and Environmental Geology
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• v.48 no.6
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• pp.501-508
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• 2015

The purpose of this study is to provide an inquiry into paleovegetation (paleoenvironment), eruption age and inferred emplacement temperature of pyroclastic deposit using charcoal occurred in the Quaternary Nari Caldera, Ulleung Island. In order to obtain the data, we carried out species identification and tree-ring analysis, radiocarbon dating for the charcoal. This sample was collected from pumiceous deposit in lowermost member(Member N-5) of the Nari Tephra Formation, which corresponds to the wood branch that has well preserved the tree-ring structure. Speciation and outermost tree-ring of wood reveal Picea spp. and $263+{\alpha}$ years. The calibrated ages from the center(pith) to the outermost tree-ring are $20,260{\pm}230$, $19,995{\pm}245$, and $19,975{\pm}265cal\;BP$, respectively, which are accordant with the tree-ring position. The youngest age, <19,710 cal BP would have implications for the eruptive age of Member N-5. Our results indicate that Picea spp. is the paleovegetation representing that Nari Caldera was under cold and wet climate conditions during the late Pleistocene. Based on the silky luster, brittleness, color, and streak of charcoal, etc., the lowest emplacement temperatures of pyroclastic flow are interpreted to have been at least as high as $350{\sim}500^{\circ}C$.

• Journal of Wetlands Research
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• v.16 no.1
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• pp.11-18
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• 2014

The effect of elevated $CO_2$ and temperature on ecological characteristics of Cicuta virosa L., the endangered plant were examined under ambient $CO_2$+ambient temperature(AC-AT), ambient $CO_2$+elevated temperature(AC-ET) and elevated $CO_2$+elevated temperature for two years. Shoot length and the number of umbels were not different in three environmental gradients. The number of tillers was high in the order of EC-ET, AC-ET and AC-AT. The number of compound umbel was the lowest in the EC-ET. Fruit set rate was the highest in the AC-AT. These results mean that unsexual propagation of C. virosa may increase by promoting growth of tillers, rather than seed production under future global warming. This population growth study will be used as the important data for the research of Korean endangered species.

• Korean Journal of Environment and Ecology
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• v.7 no.2
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• pp.112-117
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• 1994

The present conservation status on the rare and endangered plants in Tokyusan National Park were surveyed and re-evaluated by the new criteria which prepared by I.U.C.N. The species such as Lonicera vidalii, Clematis chiisanensis, Cypripedium macranthum and Allium taquetii were grouped as Critical, Eranthis stellata, Abies koreana, Taxus cuspidata, Tricyrtis dilatata, Paeonia japonaca, Stewartia koreana, Rhododendron tschnoskii, Buplerum euphorbioides, Lilium cernum and Oreorchis patens were grouped as Endangered, Adonis amurenis and Disporum ovale were grouped as Vulnerable. The above mentioned species were mainly located at the vicinity of mountain trails and projected developing sites. The potentials for the habitat destructions, mainly, due to human activities are expected to be serious in the future unless the proper management plans prepared for the specific plants and the specific habitats.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.6
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• pp.709-714
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• 2002

Fish sauce is a traditional Korean fermented seafood and has been used as a condiment since long time ago. Northern sand lance sauce was manufactured with koji, enzyme, and squid viscera of fermentation accelerating agents, and ripened at $15^{\circ}C$. Moisture contents of all samples decreased gradually as fermentation progressed. Crude protein contents increased rapidly up to 1 month-fermentation and then increased slightly up to 5 month. Northern sand lance sauce with squid viscera was the highest in the contents of protein and lipid. The ash content increased gradually during the fermentation periods, while pHs decreased. TMA contents increased up to 3 month-ripening and then decreased slightly. Amino nitrogen content of Northern sand lance sauce with squid viscera increased as fermentation progressed and was the highest among all samples. VBN contents increased up to 5 month-ripening and that of Northern sand lance sauce with squid viscera was the higher than others.

• Horticultural Science & Technology
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• v.28 no.3
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• pp.519-521
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• 2010

An Asiatic lily cultivar 'Asian Flame' was bred in 2007 at National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA) of Korea. The crossing was conducted between female parent A94-34 ('Montreux' ${\times}$ 'Anabelle') and male parent 'Fire' by stigmatic pollination at Suwon in 1996. First selection was done and tentatively named as A98-98 in 1998. After in vitro multiplication and bulb production of A98-98 line, growth and flowering characteristic tests has been conducted from 2005 to 2007. The evaluation of characteristics and preference were surveyed at a lily flower show of NIHHS in 2007 in Taean. The new cultivar 'Asian Flame' flowers in middle of June and grows to an average of 122.7 cm in height. Flowers bloom upward-facing, red (RHS 45B) petals. Year-round flowering is possible by storage of the bulb under $-1.5^{\circ}C$ conditions. For forcing culture, it is necessary to add calcium to the fertilizer or remove side scales of large bulb to prevent leaf scorch.

• Horticultural Science & Technology
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• v.28 no.3
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• pp.363-369
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• 2010

This study was carried out to investigate proper planting density and depth of tissue-cultured oriental lily bulblets for bulb production. Planting densities of bulblets were 200, 400, 600, 800, 1,000 bulblets/ $m^2$, and planting depths were 1, 3, 6, 9, 12 cm. Proper planting density of tissue-cultured 'Siberia' bulblets was 400 bulblets/ $m^2$ with 87.3% of survival rate, 6.4 cm of bulb circumference, and 8.3 g of bulb weight. For 'Sorbonne', it was thought to be 400 bulbs/ $m^2$ with 88.8% of survival rate, 5.0 cm of bulb circumference, and 7.1 g of bulb weight. Proper planting depth of tissue-cultured 'Siberia' bulbs was 3 cm with 77.8% of survival rate, 5.9 cm of bulb circumference, and 7.9 g of bulb weight. For 'Sorbonne' bulbs, it was 3 cm with 87.1% of survival rate, 5.1 cm of bulb circumference, and 6.5 g of bulb weight.