• Journal of the Computational Structural Engineering Institute of Korea
• /
• v.23 no.3
• /
• pp.289-301
• /
• 2010

A structural model of the SNF(spent nuclear fuel) disposal canister for the PWR(pressurized water reactor) for about 10,000 years long term deposition at a 500m deep granitic bedrock repository has been developed through various structural safety evaluations. The SNF disposal baskets of this canister model have the array type of which four square cross section baskets stand parallel to each other and symmetrically with respect to the center of the canister section. However whether this developed structural model of the SNF disposal canister is best is not determinable yet, because the SNF disposal canister with this parallel array has a limitation in shortening the diameter for the weight reduction due to the shortest distance between the outer corner of the square section and the outer shell. Therefore, the structural safety evaluation of the SNF disposal canister with the rotated basket array which is also symmetric with respect to the canister center planes is very necessary. Even though such a canister model has not been found as yet in the literature, the structural analysis of the canister with the rotated basket array for the PWR is required for the comparative study of the structural safety of canister models. Hence, the structural analysis of the canister with the rotated basket array in which each basket is rotated with a certain amount of degrees around the center of the basket itself and arrayed symmetrically with respect to the center planes is carried out in this paper. The structural analysis result shows that the SNF disposal canister with the rotated basket array in which the SNF disposal basket is rotated as 30~35 degrees around the center of the basket itself is structurally more stable than the previously developed SNF disposal canister with the parallel basket array.

• Proceedings of the KSME Conference
• /
• 2003.04a
• /
• pp.1754-1759
• /
• 2003

Two-dimensional flow over a pair of circular cylinders in side-by-side arrangements at low Reynolds numbers has been numerically investigated in this study. Numerical simulations are performed, using the immersed boundary method, in the ranges of $40{\leq}Re{\leq}160$ and $g^{\ast}<5$, where Re and $g^{\ast}$ are, respectively, the Reynolds number and the spacing between the two cylinder surfaces divided by the cylinder diameter. Results show that total six kinds of wake patterns are observed over the ranges: antiphase-synchronized, inphase-synchronized, flip-flopping, single bluff-body, deflected, and steady wake patterns. It is found that the characteristics of the flow significantly depends both on the Reynolds number and gap spacing, with the latter much stronger than the former. Instantaneous flow fields, time traces, flow statistics and so on are presented to identify the wake patterns and then to understand the underlying mechanism. It is remarkable that, for the deflected wake pattern, the gap flow is deflected invariably to the cylinder of higher drag coefficient and the deflection way does not change at all. Moreover, the bifurcation phenomena where either of two wake patterns can occur are found at certain flow conditions.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.27 no.7
• /
• pp.909-919
• /
• 2003

Two-dimensional flow over a pair of circular cylinders in a side-by-side arrangement at low Reynolds numbers has been numerically investigated in this study Numerical simulations are performed, using the immersed boundary method, for the ranges of 40$\leq$Re$\leq$160 and $g^{*}$＜5, where Re and $g^{*}$ are, respectively, the Reynolds number and the spacing between the two cylinder surfaces divided by the cylinder diameter. Results show that a total of six kinds of wake patterns are observed over the ranges: antiphase-synchronized, inphase-synchronized, flip-flopping, single bluff-body, deflected, and steady wake patterns. It is found that the characteristics of the flow significantly depends both on the Reynolds number and gap spacing, with the latter much stronger than the former. Instantaneous flow fields, time traces, flow statistics and so on are presented to identify the wake patterns and then to understand the underlying mechanism. Moreover, the bifurcation phenomenon where either of two wake patterns can occur is found at certain flow conditions.ons.

• Journal of the Computational Structural Engineering Institute of Korea
• /
• v.25 no.1
• /
• pp.37-49
• /
• 2012

A structural model of the SNF(spent nuclear fuel) disposal canister for the PWR(pressurized water reactor) for about 10,000 years long term deposition at a 500m deep granitic bedrock repository has been developed through various structural safety evaluations. The SNF disposal baskets of this canister model have the array type whose four square cross section baskets stand parallel to each other and symmetrically with respect to the center of the canister section. However, whether this developed structural model of the SNF disposal canister is optimal is not determinable yet. Especially, there is still a problem in weight-reduction of the canister. The cross section shape of the SNF basket should be changed to solve this problem. There are two ways in changing the cross section shape of the SNF basket; the one is to rotate the cross section itself and the other is to change the cross section shape as other shape different from the square cross section. The previous study shows that the canister with $30{\sim}35^{\circ}$ rotated basket array is structurally more stable than the canister with un-rotated parallel basket array. However, whether this canister with rotated basket array is optimal is not either determinable as yet, because it is not revealed that the canister with other cross section different from the square cross section is structurally more stable than other canisters. Therefore, the structural analysis of the SNF disposal canister with other cross section shape which is also symmetric with respect to the canister center planes is very necessary. The structural analysis of the canister with various cross section shape basket array in which each basket is arrayed symmetrically with respect to the center planes is carried out in this paper. The structural analysis result shows that the SNF disposal canister with circular cross section shape baskets located symmetrically with respect to the center of the canister section is structurally more stable than the previously developed SNF disposal canister with the parallel basket array.

• Clinical and Experimental Reproductive Medicine
• /
• v.30 no.4
• /
• pp.317-324
• /
• 2003

목 적: 본 실험의 목적은 자궁내막세포를 분리 및 배양법 확립과 함께 불멸화 시키는 것이다. 방 법: 자궁내막에서 상피세포(epithelial cells)와 기질세포(stromal cells)의 분리는 Satyawaroop 등(1979)의 방법에 기초를 두었다. 자궁내막에서 상피세포와 기질세포의 순수 분리도를 확인하고, 불멸화된 기질세포에서 SV40 large T antigen을 확인하기 위하여 면역형광 염색(immunocytochemistry)과 Western blot 기법을 이용하였다. 정상 기질세포의 경우 subconfluence (60%) 상태에서 transfection을 진행하였다. 순수 분리된 plasmid DNA와 Qiagen 사의 superfect를 이용하여 transfection을 실시하였다. 결 과: 본 연구에서 우리는 두 가지 형태의 자궁내막 세포의 분리 및 배양에 성공하였다. 상피세포는 다면체의 형태를 띠며, 선(grandular)조직의 조각으로부터 나선형으로 자란다.기질 세포는 길쭉한 형태를 띠며, 상피세포에 비해 오래 살고, 빠르게 증식하여 나란한 형태로 배열된 세포 다발(cell bundle)을 형성한다. 이렇게 분리된 세포들은 95%의 균질성을 보였으며, 면역형광염색과 western blot을 통해 확인 하였다. 한편 SV40(Simian Virus 40) large T 항원을 암호화 하고 있는 염기 서열을 포함한 플라스미드 벡터로 안정적인 트랜스펙션을 시킴으로써 불멸화 된 자궁내막의 기질 세포주를 확립하였다. 불멸화 된 세포는 그 세포가 유래한 정상의 세포와 동일한 표현형을 가지고 있었다. 결 론: 본 연구에서, 우리는 자궁내막에서 상피세포(epithelial cells)과 기질세포(stromal cells)를 분리하여 배양법을 확립하였다. 동시에 SV40 large T antigen을 이용하여 불멸화된 세포주를 확립하였다. 이렇게 확립된 세포주는 자궁의 생리작용 연구 및 자궁내막증(Endometriosis)과 자궁암(Endometrial cancer) 등과 같은 여러 자궁관련 질병 연구에 많은 도움이 될 것으로 사료된다.