• Korean Journal of Environmental Agriculture
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• v.5 no.2
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• pp.149-155
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• 1986

The fatty acid compositions of phospholipids extracted from leaves and leaf mitochondria, which were sampled from several horicultural plants grown under blue color-deficient sunlight (BCDS), were determined and compared with those from plants grown under natural white colored sunlight(WCS). It was found that the mitochondria isolated from plants grown under BCDS contained phospholipid whose degree of unsaturation in unit of number of double bonds per lipid molecule was remarkably higher than that from plants grown under WCS, the relative increment being $8{\sim}49%$. This was significantly larger than the relative increment, $4{\sim}8%$ for total phospholipid extracted from whole leaves grown under BCDS campared to WCS. This observation demonstrated that the blue light effect of sunlight on the chemical property of cellular membranes, as long as it was concerned with fatty acid composition, arose mainly at the mitochondrial membrane. Also observing that the degree of unsaturation of mitochondrial phospholipid was much lower than that of total phospholipid, it was interpreted that this was the consequence of rather active oxidative destruction of lipid-fatty acid components occuring in mitochondrial membrane by the reactive oxygen species, especially superoxide($O_2-$), which was known to be produced in mitochondrial inner membrane through the side reactions of the respiratory electron transport chain and also probably through the photosensitized reaction involving oxygen induced by blue colored light. Thus, it may be tentatively concluded that the extent of photosensitization in mitochondrial membrane could be considerably reduced under BCDS resulting in lowering of the $O_2-$ level in the respirating organelle The possible involvement of photodynamic action in membrane oxidation was also indicated by the fact that the typical fat-soluble antioxidant, ${\alpha}-tocopherol$, was found to be contained on a higher level in leaves under BCDS than those under WCS.

• The Journal of the Petrological Society of Korea
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• v.6 no.2
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• pp.133-149
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• 1997

In the metapelites around the Ogcheon granite, the metamorphic grade increases from the biotite zone through the andalusite zone to the sillimanite zone towards the intrusion contact. In the metabasites around the Boeun granite, the metamorphic grade increases from transitional zone between the greenchist and amphibolite facies through the amphibolite facies to the upper amphibolite facies towards the intrusion contact. In the Doiri area locating near the intrusion contact of the Boeun granite, sillimanite- and andalusite-bearing metapelites are found with in 500 m away from the contact. The evidence described above indicates that the Ogcheon and Boeun granites caused low-P/T type contact metamorphism to the country rocks. The P-T condition of contact metamorphism due to the intrusion of the Ogcheon granite is $540{\pm}40^{circ}C, 2.8{\pm}0.9$ kb. The temperature condition of contact metamorphism due to the intrusion of the Boeun granite is $698{\pm}28^{\circ}C$. The wide compositional range of amphibole and plagioclase in the metabasites around the Boeun granite is due to the immisibility gab of amphibole and plagioclase and unstable relict composition resulted from an incomplete metamorphic reaction. The compositional range of stable amphibole and plagioclase decreases as a metamorphic grade increases due to a close of immiscibility gab. The thermal effect of contact metamorphism due to the intrusion of the Ogcheon and Boeun granites, are calculated using the CONTACT2 program based on a two dimensional finite difference method. In order to estimate the thermal effect of an introduced pluton, a circle with 10 km diameter and a triangle with 20 km side are used for the intrusion geometries of the Ogcheon granite and the Boeun granite, respectively. The results from the field and modeling studies suggest that the intrusion temperatures of the Ogcheon granite close to $800^{\circ}C$ and the intrusion temperature of the Boeun granite is higher than $1000^{\circ}C$. However, the intrusion temperatures can be lower than the suggested temperature, if the geothermal gradient prior to the intrusion of the Ogcheon and Boeun granites was higher than the normal continental grothermal gradient.

• Korean Journal of Plant Resources
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• v.28 no.5
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• pp.574-581
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• 2015

This study describes a preliminary evaluation of the anti-inflammatory activity of Acrosorium yendoi Yamada extracts. A. yendoi Yamada was extracted using 80% ethanol and then fractionated sequentially with n-hexane, ethyl acetate and butanol. To screen for anti-inflammatory agents effectively, we first examined the inhibitory effect of 80% EtOH extract and solvent fractions of A. yendoi Yamada on the production of pro-inflammatory factors and cytokines stimulated with lipopolysaccharide. In addition, we examined the inhibitory effect of 80% EtOH extract and solvent fractions of A. yendoi Yamada on pro-inflammatory mediators (NO, iNOS, PGE₂, and COX-2) in RAW 264.7 cells. In the sequential fractions of n-hexane and EtOAc inhibited the NO and PGE₂ production and the protein level of iNOS and COX-2, and protein expression of pro-inflammatory cytokines (TNF-α, and IL-6). These results suggest that A. yendoi Yamada may have significant effects on inflammatory factors and may be provided as possible anti-inflammatory therapeutic seaweed.

• Korean Journal of Plant Resources
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• v.31 no.4
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• pp.303-311
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• 2018

In this study, a preliminary evaluation of the antioxidant and anti-inflammatory activity of the Ficus erecta var. sieboldii (Miq.) King (FES) leaf extract has been performed to assess its potential as a natural resource for food and medicinal materials. FES was extracted using 70% EtOH and then fractionated sequentially using n-hexane, $CH_2Cl_2$, EtOAc, and n-BuOH. To screen for antioxidant and anti-inflammatory agents effectively, the inhibitory effect of the FES extracts on the production of oxidant stresses (DPPH, xanthine oxidase, and superoxide) and pro-inflammatory factors (NO, iNOS, COX-2, $PGE_2$, IL-6, and $IL-1{\beta}$) in the murine macrophage cell line RAW 264.7 activated with lipopolysaccharide (LPS) was examined. Among the sequential solvent fractions of FES, the $CH_2Cl_2$ and EtOAc fractions showed decreased production of oxidant stresses (DPPH, xanthine oxidase and superoxide), and the hexane and $CH_2Cl_2$ fractions of FES inhibited the production of pro-inflammatory factors (NO, iNOS, COX-2, and $PGE_2$). The $CH_2Cl_2$ fraction also inhibited the production of pro-inflammatory cytokines ($TNF-{\alpha}$, IL-6, and $IL-1{\beta}$). These results suggest that FES has a significant effects on the production of oxidant stresses and pro-inflammatory factors and may be used a natural resource for antioxidant and anti-inflammatory agents.