• Journal of Plant Biotechnology
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• v.40 no.2
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• pp.65-71
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• 2013

Lilium cernum Komarvo. is an important endangered plant belonging to the family Liliaceae. A method was developed for the rapid micropropagation of L. cernum through plant regeneration from bulb scales explant-derived calli. The bulb scales segments were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.5, 1.0, $3.0mg{\cdot}L^{-1}$ kinetin and 0, 0.1, 0.5, $1.0mg{\cdot}L^{-1}$ NAA or 2,4-D for callus induction. In media with $0.5{\sim}3.0mg{\cdot}L^{-1}$ kinetin and $0.1{\sim}1.0mg{\cdot}L^{-1}$ NAA and 2,4-D, 95~100% of explants produced callus. They were then transferred to MS medium supplemented with various concentrations of NAA (0, 0.01, 0.05 and $1.0mg{\cdot}L^{-1}$) in combination with BA (0, 1.0 and $2.0mg{\cdot}L^{-1}$) for bulbet formation. Bulbet induction (78%), weight (468 mg) and size (15.5 mm) were obtained the highest on MS medium containing $2.0mg{\cdot}L^{-1}$ BA and $1.0mg{\cdot}L^{-1}$ NAA. In vitro frequency of plant regeneration was not significantly treated in strength of MS and sucrose concentration. Chlorophyll contents in 1/2MS with $50g{\cdot}L^{-1}$ sucrose treatments were higher than those in control and another treatment. This in vitro propagation protocol will be useful for conservation and mass propagation of this endangered plant.

• Korean Journal of Ecology and Environment
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• v.41 no.1
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• pp.98-103
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• 2008

Genetic diversity and population genetic structure within or among three stream populations (Gab, Baekgok and Ji streams) of Korean endangered natural monument fish, Iksookimia choii, were assessed by amplified fragment length polymorphism (AFLP). AFLP analysis using three primer combinations generated 104 to 106 AFLP bands, and percent polymorphic bands were similar in those three populations ranging 21.5 to 24.5%. Heterozygosity and genetic diversity within or among populations were quite low for all of these populations with average values ranging from 0.067 to 0.084 and from 0.076 to 0.087, respectively. Analyses of pairwise distance and genetic similarity among three populations of I. choii also revealed the similar results with very low genetic differentiation one another. Although pairwise Fst values were very low, our data clearly indicated distinct genetic differentiation among the three populations. This is the first report concerning the genetic diversity and differentiation of this species, and provides basic genetic information that should facilitate attempts to conserve this species.

• Proceedings of the KOR-KST Conference
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• 2001.02a
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• pp.3-29
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• 2001

본 연구에서는 기존의 대중교통 통행배정모형이 고려하지 않거나 미흡하게 반영하였던 차량과 노선의 용량제약을 고려한 모형의 구축을 시도하였다. 일반적으로 대중교통 통행배정에서 수요와 공급의 관계는 수요가 증가하는 것과 무관하다고 받아들여지고 있으나 용량 초과는 통행자들의 경로선택 및 수단선택에 영향을 끼친다고 볼 수 있다. 이러한 용량초과에 의한 혼잡을 반영하기 위해 용량제약식을 포함한 모형을 개발하고 실용운행회수라는 개념을 도입하였다. 또한 기존에 제시된 용량제약을 고려한 모형들이 실제적인 현상을 정확히 모사하는 데에는 한계가 있을 뿐 만 아니라 현실과 다소 거리가 있는 가정이 내재되어 있는 반면 본 연구에서는 가능한 한 최대로 현실 여건을 반영하는 모형을 정립하였다. 본 연구에서 제시한 모형은 동일한 기종점 사이를 운행하는 경쟁노선이 많은 대도시에서 통근·통학을 위한 통행자들이 많은 오전, 오후 첨두시 대중교통 수요를 분석하여 대중교통의 운행관리체계 개선 및 투자계획, 서비스 개선을 위한 수요예측을 하는데 유용하게 사용할 수 있을 것으로 사료된다. 그리고 기존의 대중교통 통행배정의 결과가 현재나 장래의 잠재적인 수요(demand)를 예측하는 방법인데 반하여 본 연구에서 정립된 모형은 주어진 네트워크에서 실제로 통행하고 있는 수요(flow)를 예측함으로써 교통 계획가나 대중교통 운영자의 계획 및 운영정책수립에 합리적인 도움을 줄 수 있다. 또한 현재의 대중교통 시스템 하에서의 통행배정 뿐만 아니라 다른 형태의 용량과 운행특성을 가진 교통 수단이나 노선이 도입되었을 때 공급이 제한적인 경우의 수요 예측에 적합한 모형을 개발하였다.에 대한 규제가 초국가적 차원으로 발전되는 계기를 제공하고 있다. 향후, 담배규제협약안의 세부사안들에 대한 합의과정에서 각 국별로 상당한 이견과 반발이 예상되고 있지만, 협약안의 전체 회원국 투표에서 승인될 경우 각 국가들뿐만 아니라 담배산업과 담배기업들에게 미치는 파급효과가 매우 클 것으로 예상된다. 대부분의 국제협약들이 그러하듯이, 담배규제협약도 그 적용 범위와 수준이 어느 정도로 결정되는지에 따라 각 국가와 기업별 이해관계가 크게 달라지게 되기 때문에 신중한 대응전략이 요구된다고 하겠다.의 화물전용차선의 설치시는 수답렬 교통량의 구성비와 구간 평균교통량에 의하여 그 효과가 다르게 나타남을 알 수 있었다. 따라서 물류비용 절감차원에서의 화물전용차선의 설치는 본 연구에서 나타낸 방법과 같이 수단간의 경제적 편익을 고려한 구간별 시간대별 효과분석을 통하여 정책의 시행여부가 결정되어야 할 것이다. 한편, 화물전용차선의 설치로 인한 물류비용의 절감을 보다 효과적으로 달성하기 위해서는 종합류류 전산망의 시급한 구축과 함께 화물차의 적재율을 높이고 공차율을 낮출 수 있는 운송체계의 수립이 필요한 것으로 판단된다. 그라나 이러한 화물전용차선의 효과는 단기적인 치유책일 수밖에 없기 때문에 물류유통 시설의 확충을 위한 사회간접자본의 구축을 서둘러 시행하여야 할 것이다.으로 처리한 Machine oil, Phenthoate EC 및 Trichlorfon WP는 비교적 약효가 낮았다.>$^{\circ}$E/$\leq$30$^{\circ}$NW 단열군이 연구지역 내에서 지하수 유동성이 가장 높은 단열군

• Korean Journal of Breeding Science
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• v.43 no.1
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• pp.50-55
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• 2011

The introduction of doubled haploid (DH) approach into breeding programs has reduced the times and population sizes required for the production of pure lines. We carried out the experiment for development on effective method of producing haploid in wheat. Emasculated spikelets of wheat were pollinated with maize pollen and cultured in the solution containing 40 g/L sucrose and 2,4-D, NAA, 2,4,5-T and dicamba 24 h after pollination, and then incubated until embryo rescue. Fourteen to sixteen days after pollination, the embryos are excised and cultured in half-strength MS basal medium supplemented with 20 g/L sucrose and 1 mg/L NAA. The type of plant growth regulators was found to be most significant in production of haploid plants. The application of synthetic auxins to pollinated florets, stimulates haploid embryo development to a stage where the embryos can be rescued onto nutrient media. The percentage of seeded florets was significantly affected by 100 mg/L 2,4-D, 150 mg/L 2,4,5-T and 50 mg/L dicamba. The percentage of embryos formed was significantly increased by treatment with 2,4-D and 2,4,5-T at 100 mg/L, and dicamba at 50 mg/L, but the treatments with 150 mg/L 2,4-D inhibited embryo development and plant regeneration. The optimum application time of plant growth regulators was 24 hrs after pollination.

• Journal of agriculture & life science
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• v.45 no.5
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• pp.41-47
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• 2011

This study was carried out to provide basic information for assessment conditions as habitat to conserve and restore asiatic black bear released in Jirisan National Park. The field investigation at Piagol, Bitjeom, Jangdanggol, Baemsagol, Chilseon valley, and Hanshin valley which checked for habitat of released asiatic black bear were conducted from 2008 to 2010. There were no obvious differences in home range size of asiatic black bear by study year. The seasonal home ranges were analyzed $25.64km^2$ for males and $17.57km^2$ for female in spring, $73.02km^2$ for males and $27.45km^2$ for females in summer, $41.58km^2$ for males and $13.26km^2$ for females in fall, and $6.11km^2$ for males and $2.89km^2$ for females in winter based on 95% Minimum Convex Polygon (MCP) method, respectively. In average elevation by gender of asiatic black bear, the male was higher than the female. respectively. Average elevation by season were $894{\pm}33m$ in spring, $852{\pm}20m$ in summer, $985{\pm}67m$ in fall, and $996{\pm}26m$ in winter, respectively.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.10
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• pp.459-467
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• 2019

The landing gear is a component that requires a high degree of safety to protect the lives of rotary-wing aircraft and boarding personnel, absorbing the impact on transfer/landing and supporting the fuselage during taxiing and mooring on the ground. In particular, the wheel landing gear supporting the aircraft fuselage absorbs most of the shock from the ground through the shock absorber and tires. This ensures the safety of the pilot on board the aircraft and satisfies the operational capability of the soldiers between missions. During the operation of a rotary-wing aircraft, a number of piston pins, which are a component of the right main wheel landing gear, were found to be broken. Therefore, this study examined the root cause of the piston pin crack phenomenon found in the main wheel landing gear. For this purpose, various causes were identified from fracture surface analysis of a flight test. In particular, the possibility of cracking was analyzed based on the influence on the fastening torque with the drag beam component applied to the piston pin at the time of development. This ensures the fatigue life and structural integrity.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.39 no.5
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• pp.605-612
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• 2019

Traditionally, travel demand forecasts have been conducted based on the data collected by a survey of individual travel behavior, and their limitations such as the accuracy of travel demand forecasts have been also raised. In recent, advancements in information and communication technologies are enabling new datasets in travel demand forecasting research. Such datasets include data from global positioning system (GPS) devices, data from mobile phone signalling, and data from call detail record (CDR), and they are used for reducing the errors in travel demand forecasts. Based on these background, the objective of this study is to assess the feasibility of CDR as a base data for travel demand forecasts. To perform this objective, CDR data collected for Daegu Metropolitan area for four days in April including weekdays and weekend days, 2017, were used. Based on these data, we analyzed the correlation between CDR and travel demand by travel survey data. The result showed that there exists the correlation and the correlation tends to be higher in discretionary trips such as non-home based business, non-home based shopping, and non-home based other trips.

• Korean Journal of Plant Resources
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• v.28 no.2
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• pp.253-258
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• 2015

In South Korea, Pinus pumila (Pall.) Regel (dwarf stone pine) has been designated as a critically endangered species by the Korea Forest Service. We have difficulties in obtaining the seeds of P. pumila because P. pumila grows only in the Daecheongbong area (1550–1700 m above sea level) of Mt. Seorak and almost all of its cones are damaged by birds and rodents. For establishing an ex situ conservation stand of P. pumila, this study was conducted to figure out the effects of wet cold (cold stratification, prechilling) and GA₃ treatment on the germination of P. pumila seeds. After cold stratification (1, 2, 3, 4, 5 months), prechilling (1, 2, 3, 4, 5 months) and GA₃ treatment (0, 100, 500, 1,000, 2,000, 3,000 ㎎/L), seeds were placed on petri-dishes at 25℃ under light condition. The percentage of germination, mean germination time and the germination rate were investigated. The results showed that both of the cold stratification and prechilling were effective in improving germination performances. However, there were no significant differences in performances between the two cold treatments. Within each treatment, the germination performances improved with the period of treatment. However, after three months of treatment, the performances showed no significant improvement. The gibberellin treatment was also effective in improving seed germination of P. pumila. The percentage of germination reached 79.0% in the seeds treated with 100 ㎎/L of GA₃. However, the germination performances decreased at high concentration of GA₃ treatments (over 2000 ㎎/L). In conclusion, cold stratification (over 3 months) or 100 ㎎/L of GA₃ treatment was considered to be the appropriate method for seedling production of P. pumila.

• Korean Journal of Plant Resources
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• v.23 no.2
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• pp.197-205
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• 2010

This study was carried out to investigate the flora and vegetation in wetland of Mueuido. The forest vegetation was classified into 2 communities group (Utricularia bifida-Eriocaulon sikokianum and Alnus japonica) and 6 communities (Rhynchospora chinensis, Phragmites communis, Molinia japonica, Ischaemum crassipes, Quercus serrata and Pinus rigida-Miscanthus sinesis). Resources vascular plants were composed of 30 families, 53 genera, 50 species and 8 varieties, totaling 58 taxa. Among the investigated resources plants, Utricularia bifida and Utricularia racemosa were rare and endangered species. The special plants based on floral region by Ministry of Environment were 5 taxa; V rank species 2 taxa, II rank species 1 taxa and I rank species 2 taxa. A naturalized plants was 1 species of totaling 290 species appeared in South Korea. The Life-form were Hydatophytes (20.7%), Geophyte (19%), Microphanerophytes (19%), Hemicryptophytes (17.2%), Nanophanerophytes (17.2%), Chamaephytes (5.2%), Therophytes (1.7%). In marsh-type wetland of Mueuido, we predict that bog-type swamp, where various type of swamp plants are distributed, is starting to be formed based on the fact that Utricularia bifida and Utricularia racemosa, insectivorous plants, are dominant and sphagnum also is found. Along with the notion, there are reportedly found serious damages around wetland by human being and we have to prepare protection plans against it.

• Korean Journal of Breeding Science
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• v.42 no.3
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• pp.226-230
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• 2010

'Gwanghan' (Avena sativa L.), a winter oat for forage use, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, NICS, RDA in 2009. It was derived from a cross between 'Early80' and 'Gwiri23'. A parent, 'Early80', has early heading and high yielding, while Gwiri23 has mid-heading with large-size grain. Subsequent generations followed by the cross were handled in bulk and pedigree selection programs at Suwon and Yeoncheon, respectively. A line, 'SO96016-B-112-YB-12-7', was selected for earliness, cold tolerance, and good agronomic characteristics and designated as a line name of 'Gwiri64'. The line was subsequently evaluated for cold tolerance, earliness, and forage yield in four different locations, Yesan, Iksan, Kimjae, and Jeju, from 2007 to 2009, and finally named as 'Gwanghan'. Over 3 years, the average forage dry matter yield of 'Gwanghan' harvested at milk-ripe stage was $16.4MT\;ha^{-1}$, compared with $15.4MT\;ha^{-1}$ of check cultivar 'Samhan'. 'Gwanghan's' heading date is about 3 days earlier than that of check cultivar, and is adapted primarily for winter cultivation in the mid-southern regions of Korean peninsula.