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Geological Characteristics of Extra Heavy Oil Reservoirs in Venezuela (베네주엘라 초중질유 저류층 지질 특성)

  • Kim, Dae-Suk;Kwon, Yi-Kyun;Chang, Chan-Dong
    • Economic and Environmental Geology
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    • v.44 no.1
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    • pp.83-94
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    • 2011
  • Extra heavy oil reservoirs are distributed over the world but most of them is deposited in the northern part of the Orinoco River in Venezuela, in the area of 5,500 $km^2$, This region, which has been commonly called "the Orinoco Oil Belt", contains estimated 1.3 trillion barrels of original oil-in-place and 250 billion barrels of established reserves. The Venezuela extra heavy oil has an API gravity of less than 10 degree and in situ viscosity of 5,000 cP at reservoir condition. Although the presence of extra heavy oil in the Orinoco Oil Belt has been initially reported in the 1930's, the commercial development using in situ cold production started in the 1990's. The Orinoco heavy oil deposits are clustered into 4 development areas, Boyaco, Junin, Ayachoco, and Carabobo respectively, and they are subdivided into totally 31 production blocks. Nowadays, PDVSA (Petr$\'{o}$leos de Venzuela, S.A.) makes a development of each production block with the international oil companies from more than 20 countries forming a international joint-venture company. The Eastern Venezuela Basin, the Orinoco Oil Belt is included in, is one of the major oil-bearing sedimentary basins in Venezuela and is first formed as a passive margin basin by the Jurassic tectonic plate motion. The major source rock of heavy oil is the late Cretaceous calcareous shale in the central Eastern Venezuela Basin. Hydrocarbon materials migrated an average of 150 km up dip to the southern margin of the basin. During the migration, lighter fractions in the hydrocarbon were removed by biodegradation and the oil changed into heavy and/or extra heavy oil. Miocene Oficina Formation, the main extra heavy oil reservoir, is the unconsolidated sand and shale alternation formed in fluvial-estuarine environment and also has irregularly a large number of the Cenozoic faults induced by basin subsidence and tectonics. Because Oficina Formation has not only complex lithology distribution but also irregular geology structure, geological evolution and characteristics of the reservoirs have to be determined for economical production well design and effective oil recovery. This study introduces geological formation and evolution of the Venezuela extra heavy oil reservoirs and suggest their significant geological characteristics which are (1) thickness and geometry of reservoir pay sands, (2) continuity and thickness of mud beds, (3) geometry of faults, (4) depth and geothermal character of reservoir, (5) in-situ stress field of reservoir, and (6) chemical composition of extra heavy oil. Newly developed exploration techniques, such as 3-D seismic survey and LWD (logging while drilling), can be expected as powerful methods to recognize the geological reservoir characteristics in the Orinoco Oil Belt.

Gene Expression as Related to Ripening in High Temperature during Different Coloration Stages of 'Haryejosaeng' and 'Shiranuhi' Mandarin Fruits (온주밀감 '하례조생'과 '부지화' 과실의 착색 단계별 고온에 의한 성숙 관련 유전자의 발현 변화)

  • Ahn, Soon Young;Kim, Seon Ae;Moon, Young-Eel;Yun, Hae Keun
    • Horticultural Science & Technology
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    • v.34 no.5
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    • pp.665-676
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    • 2016
  • As high temperature during citrus growing season has caused a serious problems including inferior coloration in production of mandarins in Korea, we were to investigate the expression pattern of several genes related with coloration during the ripening in high temperature condition of citrus fruits. The expression of genes related with sugar metabolism, cell wall degradation, and flavonoid synthesis in high temperature conditions was investigated in fruits of 'Haryejosaeng' (Citrus unshiu) and 'Shiranuhi' mandarin (C. reticulata). While the expression of beta-amylase (BMY), phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), and flavanone 3-hydroxylase (F3H) was differently induced, expression of polygalacturonase (PG) decreased dependently on temperature conditions. In 'Haryejosaeng' mandarin, while the expression of genes related to the skin coloration, such as CHS and F3H genes increased at $25^{\circ}C$, the expression of PAL and stilbene synthase (STS) genes were induced at $30-35^{\circ}C$ in all ripening stages. In 'Shiranuhi' mandarin, the expression of the BMY gene decreased at early time point in all temperature condition and then increased at $30-35^{\circ}C$ than at $25^{\circ}C$ in the ripening stage 2 to 3 of fruits. F3H and STS genes also showed the tendency to decrease at $30-35^{\circ}C$. Although the expression levels of genes in ripening stage 1 and stage 2 of fruits showed similar patterns in both 'Haryejosaeng' and 'Shiranuhi', the expression levels of genes were down-regulated in late ripening stage of 'Shiranuhi' fruits compared to 'Haryejosaeng'. In general, the mRNA levels of seven tested genes were higher in 'Haryejosaeng' than in 'Shiranuhi' mandarin, and expression of genes by high temperature was regulated sensitively in 'Haryejosaeng' compared to 'Shiranuhi' mandarin. Further investigations of expression of various genes based on transcriptome analysis in early ripening stage can provide valuable information about the responses to climatic changes in ripening citrus fruits.

Sexual Reproduction in Unicellular Green Alga Chlamydomonas (수염녹두말속(Chlamydomonas) 단세포 녹조의 유성생식)

  • Lee, Kyu Bae
    • Journal of Life Science
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    • v.27 no.1
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    • pp.100-121
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    • 2017
  • The sexual reproduction of the unicellular green alga Chlamydomonas is reviewed for a comprehensive understanding of the complex processes. The sexual life cycle of C. reinhardtii is distinguished into five main stages: gametogenesis, gamete activation, cell fusion, zygote maturation, and meiosis and germination. Gametogenesis is induced by nitrogen starvation in the environment. C. reinhardtii has two mating types: mating type plus ($mt^+$) and mating type minus ($mt^-$), controlled by a single complex mating type locus ($MT^+$ or $MT^-$) on linkage group VI. In the early gametogenesis agglutinins are synthesized. The $mt^+$ and $mt^-$ agglutinins are encoded by the autosomal genes SAG1 (Sexual AGglutination1) and SAD1 (Sexual ADhesion1), respectively. The agglutinins are responsible for the flagellar adhesion of the two mating type of gametes. The flagellar adhesion initiates a cAMP mediated signal transduction pathways and activates the flagellar tips. In response to the cAMP signal, mating structures between two flagella are activated. The $mt^+$ and $mt^-$ gamete-specific fusion proteins, Fus1 and Hap2/Gcs1, are present on the plasma membrane of the two mating structures. Contact of the two mating structures leads to develop a fertilization tubule forming a cytoplasmic bridge between the two gametes. Upon fusion of nuclei and chloroplasts of $mt^+$ and $mt^-$ cells, the zygotes become zygospores. It is notable that the young zygote shows uniparental inheritance of chloroplast DNA from the $mt^+$ parent and mitochondrial DNA from the $mt^-$ parent. Under the favorable conditions, the zygospores divide meiotically and germinate and then new haploid progenies, vegetative cells, are released.

Experimental Models of Schizophrenia (정신분열병의 실험적 모델)

  • Cheon, Jin-Sook
    • Korean Journal of Biological Psychiatry
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    • v.6 no.2
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    • pp.153-160
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    • 1999
  • Animal models can provide a useful tool for the study of some aspects of psychiatric disorders and their treatment. The four criteria for the evaluation of animal models of psychiatric disorders are as following : 1) similarity of inducing conditions 2) similarity of behavioral state 3) common underlying neurobiological mechanisms 4) reversal by clinically effective treatment techniques. Several animal models have been proposed for schizophrenia : phenylethylamine model, L-dopa model, hallucinogen model, cocaine model, amphetamine model, phencyclidine model, noradrenergic reward system lesion model, reticular stimulation model, social isolation model, conditioned avoidance reaction, catalepsy test, paw test, self-stimulation paradigms, latent inhibition paradigms, blocking paradigms, prepulse inhibition of the startle reflex, rodent interaction, social behavior in monkeys, hippocampal damage, high ambient pressure, and models using selective breeding. Among them, animals with bilateral lesion of the hippocampus may provide an adequate animal model for several symptoms of schizophrenia, and ketamine model can reproduce negative symptoms and cognitive deficits as well as positive symptoms of schizophrenia. In conclusion, no model of schizophrenia is entirely representative of the disease, and findings gleaned from model systems must be cautiously interpreted. Furthermore, the process of developing and validating animal models must work in concert with the process to identify reliable measures of human phenomenology.

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Growth, Storage and Fresh-cut Characteristics of Onion (Allium cepa L.) in Unstable Environmental Condition and Storage Temperature (양파의 이상 재배조건에서 생육과 저장온도에 따른 저장성 및 포장한 신선편이 특성)

  • Lee, Jung-Soo;Chang, Min-Sun;Park, SuHyoung
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.22 no.3
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    • pp.143-154
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    • 2016
  • The purpose of this study was investigated the quality changes before and after harvesting, storage and, processing of onion. Experiments were carried out to compare the effect on the characteristics of the postharvest from preharvest factors using onion. This experiment had identified the characteristics of harvested onions after cultivating with several preharvest factors such as the light and water conditions. These tests were conducted in an onion growth in the field, storage, and processing of fresh-cut during a laboratory periods of 2 years. In first year, onion cultivars ('Kars' and 'Pop') were produced under stable or unstable environment conditions, these onions were stored at low temperature(0?). Measurement was evaluated by the growth amount after harvesting, and the fresh weight loss and respiration rate during storage. According to different culture conditions and storage temperatures, it was investigated the properties of the fresh-cut onion. Growth of onion was varied depending on the cultivars and culture conditions. The amount of growth on 'Kars' and 'Pop' onions were decreased by excessive soil water conditions with shading. These influences were found the morphological differences resulting for the cell tissue of onion being rough and large. Onion cultivated in excessive soil water with shading affected the degree of its respiration rate and fresh weight loss during storage. Ones in excessive soil water with shading were higher than the control in fresh weight loss and respiration rate, respectively. However fresh-cut onion could not investigated to clarify the difference due to effects of cultivation condition and storage temperature on some measure items such as electrolyte leakage and microbial number change. There was a change of only electrolyte leakage depending on the storage temperature, rather than cultivated conditions before harvesting factor. The results showed that the onion grown on in the good environment was represented to a good quality produce even after harvesting.

Current status of Brassica A genome analysis (Brassica A genome의 최근 연구 동향)

  • Choi, Su-Ryun;Kwon, Soo-Jin
    • Journal of Plant Biotechnology
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    • v.39 no.1
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    • pp.33-48
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    • 2012
  • As a scientific curiosity to understand the structure and the function of crops and experimental efforts to apply it to plant breeding, genetic maps have been constructed in various crops. Especially, in the case of Brassica crop, genetic mapping has been accelerated since genetic information of model plant $Arabidopsis$ was available. As a result, the whole $B.$ $rapa$ genome (A genome) sequencing has recently been done. The genome sequences offer opportunities to develop molecular markers for genetic analysis in $Brassica$ crops. RFLP markers are widely used as the basis for genetic map construction, but detection system is inefficiency. The technical efficiency and analysis speed of the PCR-based markers become more preferable for many form of $Brassica$ genome study. The massive sequence informative markers such as SSR, SNP and InDels are also available to increase the density of markers for high-resolution genetic analysis. The high density maps are invaluable resources for QTLs analysis, marker assisted selection (MAS), map-based cloning and comparative analysis within $Brassica$ as well as related crop species. Additionally, the advents of new technology, next-generation technique, have served as a momentum for molecular breeding. Here we summarize genetic and genomic resources and suggest their applications for the molecular breeding in $Brassica$ crop.

Radio-isotope Red Cell Survival Studies Recommended by ICSH (방사성동위원소(放射性同位元素)를 이용(利用)한 적혈구수명측정법(赤血球壽命測定法)(ICSH추천(推薦)))

  • The Korea Society of Nuclear Medicine The Korea Society of Nuclear Medicine
    • The Korean Journal of Nuclear Medicine
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    • v.5 no.1
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    • pp.1-10
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    • 1971
  • 혈액질환(血液疾患), 특히 용혈성빈혈(溶血性貧血)을 수반(隨伴)한 경우(境遇)에 적혈구(赤血球)의 생성(生成) 및 파괴과정(破壞過程)을 정확(正確)히 파악(把握)하는 것은 중요(重要)하여 특히 적혈구수명측정(赤血球壽命測定)은 빈혈(貧血)의 본능(本能) 및 발생기전(發生機轉)을 이해(理解)하는데는 물론 병인적(病因的) 치료(治療) 및 예후(豫後)를 결정(決定)하는데 대단(犬端)히 유용(有用)하다. 적혈구수명측정(赤血球壽命測定)에는 1919년(年) Ashby가 개발(開發)한 differential agglutination법(法)이 이용(利用)되어 왔으나 수혈(輸血)에 따른 위험(危險)이 있고 방법(方法)이 복잡(複雜)하다는 단점(短點)을 가져 새로운 적혈구수명측정법(赤血球壽命測定法)이 연구(硏究)되어 왔다. 최근(最近)에 $^{51}Cr$이나, $^{32}DFP$같은 방사성동위원소(放射性同位元素)를 이용(利用)한 방법(方法)이 도입(導入)된 이래(以來) 임상적(臨床的)으로 적혈구수명측정(赤血球壽命測定)이 많이 시행(施行)되고 있지만 아직까지도 그 방법(方法)이나 결과(結果)의 해석(解釋)에 표준화(標準化)가 안되어 있다. 현재(現在) 임상영역(臨床領域)에서 가장 널리 이용(利用)되고 있는 적혈구수명측정법(赤血球壽命測定法)은 방사성(放射性) chromium($^{51}Cr$)법(法)으로 1950년(年) Gray와 Sterling에 의(依)해 창안(創案)된 이래(以來) 많은 학자(學者)들에 의(依)해 여러 가지 변법(變法)이 고안(考案)되어 왔는데, 이의 가장 큰 이유(理由)는 $^{51}Cr$이 적혈구표지(赤血球標識)에 가장 이상적(理想的)인 것만은 아니고 그 결과(結果)에 많은 요인(要因)들이 영향(影響)을 미치기 때문이다. 또 이런 변법(變法)의 사용(使用)은 각(各) 검사(檢査)에서 계산(計算)된 측정치(測定値)에 차이(差異)가 있어 그 결과(結果)의 해석(解釋) 및 비교(比較) 검토(檢討)에 적지않은 난점(難點)이 생겨 표준화(標準化)된 공통적(共通的)인 방법(方法)의 사용(使用)이 중요(重要)하다는 사실(事實)이 인식(認識)되게 되었다. 1966년(年) 호주(濠洲)의 Sydney에서 개최(開催)되었든 제11차(第11次) 국제혈액학회(國際血學會)때 열린 제4차(第4次) International Committee for Standardization in Haematology(ICSH)에서 Diagnostic Applications of Radioisotopes in Haematology에 관(關)한 expert panel을 갖을것을 의결(議決)하여 다음과 같은 12명(名)의 위원(委員)이 결정(決定)되었으며 위원회(委員會)의 의장(議長)에 Dr. Szur, 총무(總務)에 Dr. Glass가 각각(各各) 선임(選任)되었다. 그간(間) 1967년(年) 영경(英京) London에서 첫 회합(會合)이 있은후(後) New York, Vienna(IAEA후원(後援)) Brthesda(NIH후원(後援))에서 전문위원회(專門委員會)를 갖고 적혈구수명측정법(赤血球壽命測定法)에 관(關)한 의견(意見)의 일치(一致)를 보았다. ICSH와 국제혈액학회(國際血學會)에서는 이번에 결정(決定)된 적혈구수명측정법(赤血球壽命測定法)을 널리 소개(紹介)하며, 측정법(測定法)과 얻어진 결과(結果)의 해석(解釋)에 표준화(標準化)를 기(期)할 목적(目的)으로 이에 연관성(聯關性)있는 전문지(專門誌)에 게재(揭載)할 것을 요청(要請) 받었기에 이에 전문(全文)을 소개(紹介)하는 바이다. 이들은 방사성(放射性) chromium 법(法)의 모든 세부적(細部的)인 면(面)을 표준화(標準化)하고 있으며 그간(間) 가장 논란(論難)의 대상(對象)이 되었던, $^{51}Cr$-표지방법(標識方法)에 있어서의 세가지 변법(變法), 즉 ACD법(法), Citrate-wash법(法), ACD/ascorbic acid법(法)을 모두 인정(認定)하고 있다. 또한, DFP($DF^{32}P$ 또는 $^3H-DFP$) 표지법(標識法)의 표준방법(標準方法)도 기술(記述)하고 있으며, 더욱 중요(重要)한 것은 적혈구수명측정법(赤血球壽命測定法)으로서 현재(現在)까지 대부분(大部分)의 학자(學者)가 사용(使用)하여 왔던 지표(指標)인 $T_{50}Cr$ 대신(代身)에 mean red cell life span을 사용(使用)할 것을 권(勸)하고 있다.

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A Case Study on the classroom life and the identity of the Elementary Mathematics Gifted Education (초등수학 영재교육원의 교실 생활과 정체성에 대한 사례연구)

  • Lee, Hak-Ro;Ryu, Sung-Rim
    • Communications of Mathematical Education
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    • v.25 no.1
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    • pp.99-118
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    • 2011
  • For this case study of gifted education, two classrooms in two locations, show life in general of the gifted educational system. And for this case study the identity of teachers and the gifted, help to activate the mathematically gifted education for these research questions, which are as followed: Firstly, how is the gifted education classroom life? Secondly, what kind of identity do the teachers and gifted students bring to mathematics, mathematics teaching and mathematics learning? Being selected in the gifted children's education center solves the research problem of characteristic and approach. Backed by the condition and the permission possibility, 2 selected classes and 2 people, which are coming and going. Gifted education classroom life, the identity of teachers and gifted students in mathematics and mathematics teaching and mathematic learning. It will be for 3 months, with various recordings and vocal instruction between teacher and students. Collected observations and interviews will be analyzed over the course of instruction. The results analyzed include, social participation, structure, and the formation of the gifted education classroom life. The organization of classes were analyzed by the classes conscious levels to collect and retain data. The classes verification levels depended on the program's first class incentive, teaching and learning levels and understanding of gifted math. A performance assessment will be applied after the final lesson and a consultation with parents and students after the final class. The six kinds of social participation structure come out of the type of the most important roles in gifted education accounts, for these types of group discussions and interactions, students must have an interaction or individual activity that students can use, such as a work product through the real materials, which release teachers and other students for that type of questions to evaluate. In order for the development of meaningful mathematical concepts to formulate, mathematical principles require problem solving among all students, which will appear in the resolution or it will be impossible to map the meaning of the instruction from which it was formed. These results show the analysis of the mathematics, mathematics teaching, mathematics learning and about the identity of the teachers and gifted. Gifted education teachers are defined by gifted math, which is more difficult and requires more differentiated learning, suitable for gifted students. Gifted was defined when higher level math was created and challenged students to deeper thinking. Gifted students think that gifted math is creative learning and they are forward or passive to one-way according to the education atmosphere.

Effects of Baicalin on Gene Expression Profiles during Adipogenesis of 3T3-L1 Cells (3T3-L1 세포의 지방세포형성과정에서 Baicalin에 의한 유전자 발현 프로파일 분석)

  • Lee, Hae-Yong;Kang, Ryun-Hwa;Chung, Sang-In;Cho, Soo-Hyun;Yoon, Yoo-Sik
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.1
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    • pp.54-63
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    • 2010
  • Baicalin, a flavonoid, was shown to have diverse effects such as anti-inflammatory, anti-cancer, anti-viral, anti-bacterial and others. Recently, we found that the baicalin inhibits adipogenesis through the modulations of anti-adipogenic and pro-adipogenic factors of the adipogenesis pathway. In the present study, we further characterized the molecular mechanism of the anti-adipogenic effect of baicalin using microarray technology. Microarray analyses were conducted to analyze the gene expression profiles during the differentiation time course (0 day, 2 day, 4 day and 7 day) in 3T3-L1 cells with or without baicalin treatment. We identified a total of 3972 genes of which expressions were changed more than 2 fold. These 3972 genes were further analyzed using hierarchical clustering analysis, resulting in 20 clusters. Four clusters among 20 showed clearly up-regulated expression patterns (cluster 8 and cluster 10) or clearly down-regulated expression patterns (cluster 12 and cluster 14) by baicalin treatment for over-all differentiation period. The cluster 8 and cluster 10 included many genes which enhance cell proliferation or inhibit adipogenesis. On the other hand, the cluster 12 and cluster 14 included many genes which are related with proliferation inhibition, cell cycle arrest, cell growth suppression or adipogenesis induction. In conclusion, these data provide detailed information on the molecular mechanism of baicalin-induced inhibition of adipogenesis.

A Study of the Characteristics of Heavy Rainfall in Seoul with the Classification of Atmospheric Vertical Structures (대기연직구조 분류에 따른 서울지역 강한 강수 특성 연구)

  • Nam, Hyoung-Gu;Guo, Jianping;Kim, Hyun-Uk;Jeong, Jonghyeok;Kim, Baek-Jo;Shim, Jae-Kwan;Kim, Byung-Gon
    • Journal of the Korean earth science society
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    • v.40 no.6
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    • pp.572-583
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    • 2019
  • In this study, the atmospheric vertical structure (AVS) associated with summertime (June, July, and August) heavy rainfall in Seoul was classified into three patterns (Loaded Gun: L, Inverted V: IV, and Thin Tube: TT) using rawinsonde soundings launched at Osan from 2009 to 2018. The characteristics of classified AVS and precipitation property were analyzed. Occurrence frequencies in each type were 34.7% (TT-type), 20.4% (IV-type), 20.4% (LG-type), and 24.5% (Other-type), respectively. The mean value of Convective Available Potential Energy (1131.1 J kg-1) for LG-types and Storm Relative Helicity (357.6 ㎡s-2) for TT-types was about 2 times higher than that of other types, which seems to be the difference in the mechanism of convection at the low level atmosphere. The composited synoptic fields in all cases showed a pattern that warm and humid southwesterly wind flows into the Korean Peninsula. In the cases of TT-type, the low pressure center (at 850 hPa) was followed by the trough in upper-level (at 500 hPa) as the typical pattern of a low pressure deepening. The TT-type was strongly influenced by the low level jet (at 850 hPa), showing a pattern of connecting the upper- and low-level jets. The result of analysis indicated that precipitation was intensified in the first half of all types. IV-type precipitation induced by thermal instability tended to last for a short term period with strong precipitation intensity, while TT-type by mechanical instability showed weak precipitation over a long term period.