• Korean journal of applied entomology
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• v.51 no.1
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• pp.47-58
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• 2012

Effect of a new crop protectant 'Bt-Plus' on natural enemies was analyzed in this study. Tested natural enemies included two parasitic species of $Aphidius$ $colemani$ and $Eretmocerus$ $eremicus$, and four predatory species of $Harmonia$ $axyridis$, $Orius$ $laevigatus$, $Amblyseius$ $swirskii$, and $Phytoseiulus$ $persimilis$. 'Bt-Plus' was formulated by combination of three entomopathogenic bacteria ($Xenorhabdus$ $nematophila$ (Xn), $Photorhabdus$ $temperata$ subsp. $temperata$ (Ptt), $Bacillus$ $thuringiensis$ (Bt)) and bacterial metabolite (BM). All three types of 'Bt-Plus' showed significantly higher toxicities against fourth instar $Plutella$ $xylostella$ larvae than Bt single treatment. Two types of bacterial mixtures ('Xn+Bt' and 'Ptt+Bt') showed little toxicity to all natural enemies in both contact and oral feeding assays. However, 'BM+Bt' showed significant toxicities especially to two predatory mites of $A.$ $swirskii$ and $P.$ $persimilis$. The acaricidal effects of different bacterial metabolites were evaluated against two spotted spider mite, $Tetranychus$ $urticae$. All six BM chemicals showed significant acaricidal effects. The BM mixture used to prepare 'Bt-Plus' showed a high acaricidal activity with a median lethal concentration at 218.7 ppm (95% confidence interval: 163.2 - 262.3). These toxic effects of bacterial metabolites were also proved by cytotoxicity test against Sf9 cells. Especially, benzylideneacetone, which was used as a main ingredient of 'BM+Bt', showed high cytotoxicity at its low micromolar concentration.

• Korean journal of applied entomology
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• v.19 no.4 s.45
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• pp.187-192
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• 1980

This study was carried out to investigate the bionomics of Ostrinia furnacalis $(Gu\grave{e}n\grave{e}e)$ in the laboratory and field in 1978. The oriental corn borer had three generations a year in Korea ana the first period of moth activity was mostly from early June to early July, the second from mid July to mid August and the third from mid August to early September. It overwintered with larval stage in the corn stubbles. The egg period was 3-4 days. The larva molted 5-6 times and its period was 18-30 days. The longevity of adult was 7-11 days and deposited about 600-800 eggs. An egg parasite and two larval parasites were investigated.

• Korean journal of applied entomology
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• v.28 no.4
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• pp.221-228
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• 1989

The oviposition behavior of Ooencyrtus kuvanae (Howard) (Hymenoptera: Encyridae) was examined in a small petri dish containing eggs of gypsy moth, Lymantrt'a dt'spar L. The behavioral sequence from initial searching movement toward the host egg through oviposition, departure, and auxiliary phase activities such as resting, glooming, and host feeding were described. Oviposition experience shortened the approach time to subsequent eggs. The time spent for contacting the host egg also decreased with oviposition experience. Oviposition experience may play an important role in host recognition and increasing oviposition efficiency.

• Korean journal of applied entomology
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• v.62 no.4
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• pp.365-369
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• 2023

The genus Bitomus Szepligeti, 1910 (Hymenoptera: Braconidae: Opiinae) is known to parasitize in mining flies (Diptera: Agromyzidae), of which four species are recorded in Korea. Bitomus was revised by Fischer (1987, 1994) and recently treated by Papp (1978, 1981) in Korea. Bitomus is distinguished from other genera by the immovably joined second-third or fourth metasomal tergites, forming a carapace. In this study, Bitomus indicus is recognized for the first time in Korea. This study provides a diagnosis, description, distribution, and diagnostic illustrations.

• Korean journal of applied entomology
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• v.40 no.4
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• pp.301-307
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• 2001

The pine needle gall midge (PNGM), Thecodiplosis japonensis Uchida et Inouye, is a serious pest in pine forests in Korea. Since the first report of PNGM infestation in Korea in 1929, the infestation area has been expanded gradually. In 1975 the distribution of PNGM and its parasitoids were surveyed throughout several infested areas in Korea. Annual survey has been made for the rates of gall formation by PNGM and parasitism by its parasitoids at the monitoring sites including newly infested area in 1975 since 1980. These data were used to examine the relationship between PNGM and its parasitoids for newly invaded areas. The gall forming rate of PNGM and the parasitism by the parasitoid were 34.8 and 1.9% in 1975, respectively, while the gall forming rate and the parasitism were 20.7 and 8.9% in 1985, respectively. The relationships of densities between PNGM and its parsitoid were weak in the early stage of dispersion, but the density of parasitoid was with an asymptotic increase along with PNGMs density increase during the observation period for 10 years.

• Korean journal of applied entomology
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• v.48 no.2
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• pp.123-158
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• 2009

An attempt was made to stimulate future research by providing exemplary information, which would integrate published knowledge to solve specific pest problem caused by resistance. This review was directed to find a way for delaying resistance development with consideration of chemical(s) nature, of mixture, rotation, or mosaics, and of insecticide(s) compatible with the biological agents in integrated pest management (IPM). The application frequency, related to the resistance development, was influenced by insecticide activity from potentiation, residual period, and the vulnerability to resistance development of chemical, with secondary pest. Chemical affected feeding, locomotion, flight, mating, and predator avoidance. Insecticides with negative cross-resistance by the difference of target sites and mode of action would be adapted to mixture, rotation and mosaic. Mixtures for delaying resistance depend on each component killing very high percentage of the insects, considering allele dominance, cross-resistance, and immigration and fitness disadvantage. Potential disadvantages associated with mixtures include disruption of biological control, resistance in secondary pests, selecting very resistant population, and extending cross-resistance range. The rotation would use insecticides in high and low doses, or with different metabolic mechanisms. Mosaic apply insecticides to the different sectors of a grid for highly mobile insects, spray unrelated insecticides to sedentary aphids in different areas, or mix plots of insecticide-treated and untreated rows. On the evolution of pest resistance, selectivity and resistance of parasitoids and predator decreased the number of generations in which pesticide treatment is required and they could be complementary to refuges from pesticides To enhance the viability of parasitoids, the terms on the insecticides selectivity and factors affecting to the selectivity in field were examined. For establishment of resistant parasitoid, migration, survivorship, refuge, alternative pesticides were considered. To use parasitoids under the pressure of pesticides, resistant or tolerant parasitoids were tested, collected, and/or selected. A parasitoid parasitized more successfully in the susceptible host than the resistant. Factors affecting to selective toxicity of predator are mixing mineral oil, application method, insecticide contaminated prey, trait of individual insecticide, sub-lethal doses, and the developmental stage of predators. To improve the predator/prey ratio in field, application time, method, and formulation of pesticide, reducing dose rate, using mulches and weeds, multicropping and managing of surroundings are suggested. Plant resistance, predator activity, selective insect growth regulator, and alternative prey positively contributed to the increase of the ratio. Using selective insecticides or insecticide resistant predator controlled its phytophagous prey mites, kept them below an economic level, increased yield, and reduced the spray number and fruits damaged.

• Korean journal of applied entomology
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• v.44 no.3 s.140
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• pp.219-223
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• 2005

Torymus geranii was found to be a ectoparasitoid attacking the larvae of Dryocosmus kuriphilus. T. geranii has longevity of a $42.8{\pm}9.8$ days at $20^{\circ}C$ and $26.5{\pm}11.0$ day at $25^{\circ}C$ under conditions supplied with 100% honeydew. Oviposition numbers at 20 and $25^{\circ}C$ were $22.3{\pm}12.5$ and $42.2{\pm}18.4$, respectively. The eggs were oval in shape, measuring $0.56{\pm}0.33$ mm in length. The larvae, $2.94{\pm}0.18mm$ in length, was white and length of male and female pupae were $2.01{\pm}0.18mm$ and $2.73{\pm}0.09mm$, respectively. T. geranii had two generations with overwintering generation emerged on late May to early June at Chunchon, central part of Korea, however showed three generations with overwintering generation emerged on middle May to early June, the first one on late June to early July, and the second one on late July to earlyl August.

• Korean Journal of Environmental Biology
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• v.40 no.4
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• pp.423-432
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• 2022

The mass-rearing system for Neodryinus typhlocybae as a biological control agent of Metcalfa pruinosa was established. Depending on the density of host nymphs and plants, the average number of cocoons produced by the parasitoids was 5-8 and 70-150 cocoons per leaf and sapling of mulberry, respectively. There is a significant difference in cocoon length between females (6.10-6.46mm) and males (4.20-4.62mm). Sex determination of cocoons before emergence will be helpful for efficiently releasing this parasitoid in fields. The parasitic rate of N. typhlocybae at the semi-field condition was on average 13-17%. The release number of this parasitoid did not affect parasitism. Nevertheless, the population growth rate of M. pruinosa was reduced by increasing the release number of N. typhlocybae. The parasitoid offspring's sex and bivoltine were influenced by the host age. On young host nymphs, the bivoltine portion of parasitoid increased. When parasitized on 4^th or 5^th nymphs, the offspring's female ratio of N. typhlocybae increased. This result may be useful for potentially controlling mass rearing production of parasitoid.

• Korean journal of applied entomology
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• v.48 no.1
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• pp.87-94
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• 2009

KNI3126 which is the eco-friendly material made by plant extracts - neem and matrine - have been evaluated for efficacies against 6 main pests and toxicity on natural enemies. Insecticidal efficacies of KNI3126 against plant hopper were above 95% at 5 days after treatment (DAT), whereas pesticidal effects against the cotton aphid were above 95% at 3 DAT and slightly decreased at 5 DAT. Insecticidal efficacy against the palm thrips resulted in lower control value as 68.1% than that of chemical insecticides. KNI3126 showed more than 95% control value against diamond back moth, suggesting that it could suppress the population of pest hard to control as eco-friendly material. Mortalities against two-spotted spider mite were about 80% at 1 DAT and over 90% at 5 DAT, respectively. For evaluation of the toxicity on natural enemies, effect against predatory natural enemy was classified as moderate selective toxicity based on the criterion of International Organization of Bio-Control (IOBC), whereas against parasitic natural enemies was found to be relatively safe. Repellent effect was weak against plant hopper, but strong against two-spotted spider mite. In the toxicity test for safety, KNI3126 was classified as the lowest level at acute oral, acute dermal and fish toxicity test and did not induce the irritancy at skin irritation test and eye irritation test.

• Korean Journal of Agricultural Science
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• v.8 no.1
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• pp.18-28
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• 1981

The purpose of this study was to establish the intergrated control of rice insect pests. Specific objectives were to reveal the diversity of natural enemies in rice field environment, to obtain basic informations of their population dynamics, and to screen the selective insecticides for the conservation of natural enemies. The results of the study were as follows. 1. In numbers of species and in numbers of each species of parasitic Hymenoptera of rice insect pests were more diverse and abundant in rice paddy leeves and banks than paddy fields. Braconid Mymarid and pteromalid were predominated in the field and their population was high during early August to mid September. 2. Of the predatory spiders, Pirata subpiraticus was the largest in number and amounted to 72% of the total, Gnatonarium dentatum, Pachygnatha clerki and Clubiona japonicola were the next. It was also found that P. subpiraticus had three generations in a year. 3. Hence the activities of the predatory spider species and parasitic Hymenoptera were high in early to mid August and September, it would be better promising to avoid chemical applications at this time of periods as possible. 4. The relative toxicity of several insecticides which have been used for the control of brown plant hopper (BPH) showed that P. subpiraticus was 1.1-73.1 times higher than BPH and G. dentatum to P. subpiraticus was 1.1-73.1 times, respectively. 5. Three conventional insecticides, Padan, Diazinon and Carbofuran were screened for toxicity to predatory spider species. The insecticides deffered in their toxicity to the predators. However, Padan was appeared to be the least toxic to the predators.