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Development of Ultra-rapid Nested PCR Method for Detection of Specific Gene of Tracheal Mite (Acarapis woodi) (기문응애(Acarapis woodi) 특이 유전자 검출을 위한 초고속 nested PCR법 개발)

  • Kim, MoonJung;Kim, Byoung-Hee;Kim, SoMin;Truong, A Tai;Kim, Jung-Min;Kim, Seonmi;Yoon, Byoung-Su
    • Journal of Apiculture
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    • v.34 no.1
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    • pp.15-26
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    • 2019
  • Tracheal mite (Acarapis woodi) is an internal parasite that is parasitic on the bronchus of adult bees and sucks fluid from the trachea. Since its first report by Rennie, it has been spread throughout Europe and in some Asian regions, with adjacent Japan and China reported in 2011 and 2012, respectively. Korea detected specific genes of A. woodi in 2015, but only one of 99 samples has been identified and the being of A. woodi has not been confirmed. In this study, we established a specific nested PCR method to confirm for detecting low-copy number of A. woodi-specific gene in bee samples. As a result, A. woodi-specific COI gene was amplified in 15 of 23 samples, and they were judged positive by melting point analysis and sequencing analysis. Although we could not observe the existence of the mites in bees, our results suggest that tracheal mit might exist in nature.

Enhanced Control Efficacy of a Fumigant, Chlorine Dioxide, by a Mixture Treatment with Carbon Dioxide (이산화탄소 혼합 처리를 통한 이산화염소 훈증 처리 효과 제고 기술)

  • Kim, Chulyoung;Kwon, Hyeok;Kim, Wook;Kim, Yonggyun
    • Korean journal of applied entomology
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    • v.56 no.3
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    • pp.253-259
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    • 2017
  • Fumigation using chlorine dioxide ($ClO_2$) has been regarded as a potential control technique against stored grain insect pests. The control efficacy can be enhanced with increase of opening rates of spiracles by facilitating the toxic gas delivery to internal body. To test this hypothesis, this study used the Indianmeal moth, Plodia interpunctella, which was known to be susceptible to $ClO_2$, and analyzed the opening rate of spiracles. A total of 9 pairs of spiracles were observed in P. interpunctella larvae, in which one pair was located on the prothorax and eight pairs were on the abdomen. Within the body, the spiracles were connected to longitudinal and transverse tracheal trunks. The open spiracles were determined by the dye-infiltration method. Based on this method, the opening rate of spiracles increased up to about 60% with increase of ambient temperatures. Furthermore, exposure to carbon dioxide stimulated the opening rate of spiracles up to about 95%. In contrast, exposure to $ClO_2$ prevented the spiracle opening and resulted in only 25% of spiracles in an opening state. The addition of carbon dioxide to $ClO_2$ treatment rescued the opening rate of spiracles as much as the carbon dioxide single treatment. Based on this modulatory activity of carbon dioxide against spiracles, the combined treatment of $ClO_2$ with carbon dioxide resulted in significant increase of its toxicity against P. interpunctella compared to a single $ClO_2$ treatment.

A Study on User Interface Using a Basic Frame of KIMUNDUNKAP "Focusing on Display Menu of Mobilephone" (기문둔갑 UI 디자인 활용방안에 관한 기초연구 "모바일폰 디스플레이 메뉴 중심으로")

  • Yi, Joong-Seok
    • 한국HCI학회:학술대회논문집
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    • 2006.02b
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    • pp.147-155
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    • 2006
  • 최근 동양사상은 사회, 문화 전반에 걸쳐 활용되고 있으며 디자인 분야에 적용하려는 연구 역시 지속적으로 이어져오고 있다. 이러한 맥락으로 볼 때 동양사상과 인터페이스의 관계에서도 음양오행, 팔괘, 하도낙서 등의 기본원리에 의한 적용이 가능하다고 유추할 수 있으며, 우주 만물의 조화와 균형을 추구하고 있는 동양사상을 사용자 인터페이스에 적용한다면 사용하기 쉬움과 어려움의 균형이 적절하게 조화를 이루어 인간이 보다 자연스럽게 사용할 수 있는 UI가 개발될 가능성을 가지고 있다고 할 수 있다. 본 연구는 구궁. 팔괘의 의미에 따른 모바일폰 메뉴 구조를 통하여 단순히 사용빈도가 높거나 유사한 항목간의 관계, 기업의 마케팅을 위한 관계 설정이 아닌 각 기능이 가지고 있는 본질적 의미를 팔괘로 해석하고, 구궁에 적용함으로써 질서와 조화를 갖춘 메뉴 관계를 형성하고, 이를 통해 인간이 보다 자연스럽게 사용 할 수 있는 UI를 제언하고자 하는 목적으로 진행되었다. 연구방법으로 음양오행, 주역의 팔괘 등을 체계적으로 수용하고 있으며, 구궁이라는 9개의 공간에 의미와 시간의 요소를 조합하여 해단함으로써, 인간사 전반에 걸친 해석이 가능한 '기문둔갑'이라는 동양학문을 제시하고, 이를 토대로 사용자 인터페이스와의 관계를 밝히고 적용가능성을 설명하였다. 사례연구로 $3{\times}3$ 메뉴구조의 모바일폰 디스플레이 인터페이스에 기문둔갑의 기초인 구궁, 팔괘를 적용하는 방법을 설명하고 디자인전공자 30명의 설문으로 얻은 사용자 멘탈모델과 비교 분석하여 디자인에서의 동양사상 활용 가능성을 설명하였다.

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An Analysis of the Choice of Wedding Time Based on the Gimundungap Theory (기문둔갑으로 보는 혼인 택일법의 사례분석)

  • Cheong-Ha Lee;Ki-Chan Kim
    • Industry Promotion Research
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    • v.8 no.1
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    • pp.119-134
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    • 2023
  • An Analysis of the Choice of Wedding Time Based on the Gimundungap Theory. The purpose of this study is to research the utility and validity of wedding time selection based on the principles of Ungokgimun, a recent development of Gimundungap theory. Unlike the conventional way of choosing from common auspicious hours and days deemed available for important ceremonial occasions, Ungokgimun determines the propitious heavenly wedding time catered for a particular couple based on the birth table consisting of the four pillars of the bride- and groom-to-be. Using the Hong Guk-soo numbers which are drawn from the basic principles of oriental philosophy, it utilizes a deductive approach to wedding time choice, which is considered decisive and logical. Wedding time selection for a bride and a bridegroom is determined by auspicious combinations of matching and supporting HongGuk-soo numbers. Some relevant determining factors for the time choice are three number combination of samhap, two number combination of half-hap, latitudinal combination of yuk-hap, support and control relationships between related elements, two number punishment of hyung-sal, and three number punishment of samhyung-sal, etc. The specific auspicious palace of the spouse-to-be's luck is selected on the basis of supporting or brotherly combination of numbers which are manifested on the baseline earthly plate of the bride- and groom-to-be. This is followed by the selection of the ten-year luck, and year and month luck, and finally by the selection of auspicious day and hours. The validity of this study was verified through theoretical consideration of the Ungokgimun and practical analysis of a variety of marriage cases. It was found that the way of wedding time selection using Ungok Gimundungap was relatively more effective than other conventional methods.

Description of the Taxonomic Terminology of Lepidoptera Larvae (나방류 유충의 분류학적 형질에 관한 연구)

  • Piao, MeiHua;Lee, Chan Young
    • Journal of Forest and Environmental Science
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    • v.14 no.1
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    • pp.14-23
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    • 1998
  • This paper gave detailed observation of the morphology of lepidoptera larvae, and viewed their charactor which is a key in the comparative study. 1. Head : ocelli, antenna, labrum, labium, mandible, maxilla, setae, frontal suture, adfrontal suture, and epicranial suture. 2. Thorax : thoracic shield, spiracle, setae and setal map. 3. Abdomen : anal shield, crochets of proleg, spiracle, setae and setal map.

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Study on 『BianZhengGiMun)』's medical characteristics - In view of ShangHan - (『변증기문(辨證奇聞)』의 의학적(醫學的) 특징(特徵)에 관(關)한 고찰(考察) -상한문(傷寒門)을 중심(中心)으로-)

  • Lee, Won-Suk;Park, Sun-Dong;Park, Won-Hwan;Kim, Jun-Ki;Kim, Jong-Dae
    • The Journal of Dong Guk Oriental Medicine
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    • v.6 no.2
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    • pp.33-85
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    • 1998
  • In order to find the characteristic medical methods of "BianZhengGiMun" by the author ChenShihTo, we have translated and analyzed the mentioned book. Upon analyses, the following facts were noted : 1) "BianZhengGiMun" is written and composed of case studies. Prescriptive methods described here differ rather from previously known methods, where as our nation's Oriental medicine practitioners refer and consider these methods as miraculous modern methodologies. 2) Book's content has much similarities to "ShangHanLun"'s original text. Moreover, the content is adjusted towards presenting methodologies not coverd in "ShangHanLun"'s text, together with adding more information to conserve "ShangHanLun"'s methods as well as to critique them. 3) Diagnostic methods mainly follow the ZangFuBianZheng(臟腑辨證)'s pattern, where it's recorded information and theories are valid. 4) "BianZhengGiMun"'s proscriptive methods usually follow BuYinShengJin(補陰生津)'s form, where they were appropriate for that period and circumstances. 5) In consideration of all of the above factors, ChenShihTo grasped ShangHan and WenBing(acute febrile disease due to exogenous factor) as WaiGanReBing(外感熱病)'s double sided characteristics, where this corresponds with the current belief. It is believed that this in turn would provide much practical value to the present time.

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A Feasibility Study on the Hour Setting in Almanac (역법에서의 시진(時辰) 설정에 대한 타당성 논의)

  • Cheong-Ha Lee;Sun-Ok Shin
    • Industry Promotion Research
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    • v.8 no.2
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    • pp.119-132
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    • 2023
  • The purpose of this paper is to re-establish the concept of Eastern time, which is used in Myeongliology and Kimun-dungap, and to discuss the use of real-time of the sun according to the birth area, the selection of real-jasi between night-jasi and real-jasi, and the exact meaning of birth time. The conventional use of time in Korean society uses Tokyo as standard time and uses a time system in which a new day begins at midnight. Howev er, what is important is not social univ ersality but the time and space of the univ erse where I was born, which is the center of the universe, should be the standard of my fate, so I have to use the longitude of the place where I was born to determine my exact time. Also it is argued that real-jasi should be selected for the selection problem of night-jasi and real-jasi, which has long been a controversial topic in Myeongliology, because the flow of cosmic energy is signaled an hour ahead. To secure the validity of setting such time, a case analysis was conducted using the time adjustment method through the spread of Kimun-dungap. Through in-depth interviews with the analysis subjects, the lives of the past were collected by date and time, and then Kimun-dungap checked whether the timing of each life event was accurate.

Trends of Scenery Research with a Old Literature Subject Matter (고문헌을 제재(題材)로 한 전통경관연구의 경향분석)

  • Kim, Myo-Jung;Jung, Ki-Ho
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.32 no.1
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    • pp.44-52
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    • 2014
  • The purpose of this study is to investigate the tendency of the research on the traditional scenery architecture based on the old literature for the last 10 years and to suggest direction of the research on the traditional scenery through utilization of the old literature. For the tendency analysis of the research on the traditional scenery, I investigated and analyzed researches published in the Journal of the Korean Institute of Traditional Landscape Architecture between 2002 and 2012, and reviewed the utilization of the old literature about research on the traditional scenery. The number of the research papers through the old literature was two or three a year by 2009, but has increased to more than eight from 2010. The utilization tendency of the old literature was mostly to use the Korean translations or to use a collection of an individual's works after partially translating. As some of the researches were carried out only through translations even when they used the original, historical evidence on the translation of the original was insufficient. I can suggest the direction of the research on the traditional scenery by the results as follows: it is necessary to present both translation and the original and to specify the source of translation, for the efficient understanding and verification of the original. Professional translation of landscape architecture and scenery also seems to be necessary for the records of the current state. For the analysis of the old literature, as the analysis of the traditional scenery both through poetry and records facilitates assumption of the location of landscape by the abundant data, it seems to be necessary to approach research, using poetry and prose complementarily, after preparing foundation of research based on the records.