• Journal of KIISE:Databases
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• v.35 no.2
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• pp.125-131
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• 2008

It is very useful to predict orthologs for new genome annotation and research on genome evolution. We showed that the previous work can be extended to construct OCs(Ortholog Clusters) automatically from multiple complete-genomes. The proposed method also has the quality of production of InParanoid, which produces orthologs from just two genomes. On the other hand, in order to predict more exactly the function of a newly sequenced gene it can be an important issue to prevent unwanted inclusion of paralogs into the OCs. We have, here, investigated how well it is possible to construct a functionally purer OCs with score cut-offs. Our OCs were generated from the datasets of 20 procaryotes. The similarity with both COG(Clusters of Orthologous Group) and KO(Kegg Orthology) against our OCs has about 90% and inclines to increase with the growth of score cut-offs.

• Korean Journal of Organic Agriculture
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• v.31 no.4
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• pp.395-412
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• 2023

The morphology and whole genome sequence of Hyphantria cunea nucleopolyhedrovirus W1 (HycuNPV-W1) isolated in Korea were analyzed for the use as an eco-friendly control agent against H. cunea. The HycuNPV-W1 had irregular tetrahedral polyhedra with a size of 1.5-2.2 ㎛ which is similar to that of previously reported HycuNPV isolated in Korea. As a result of whole viral genome analysis, HycuNPV-W1 was composed of 131,353 bp, which is 1,606 bp shorter than that of the previously reported HycuNPV. The G+C content was 45% and six of the homologous repeated regions were found, so there was no significant difference from the previous report. As a result of ORF analysis, HycuNPV-W1 contains total of 145 ORFs which is three ORFs less than the previous report, while two ORFs were exclusively found in HycuNPV-W1. The functions of these ORFs remains unclear and are not considered to have a significant influence on the characteristics of the HycuNPV. The genome vista analysis showed that the overall sequence identity between HycuNPV-W1 and the previously reported HycuNPV was very high. The whole genome of HycuNPV-W1 analyzed was found to be similar to those of the previously reported HycuNPV, however, it is supposed to be a novel resource in Korea with different isolate.

• Journal of Korea Technology Innovation Society
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• v.9 no.1
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• pp.84-102
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• 2006

This paper compares four models to value a biotechnology R&D project; option tree model, dynamic discounted cash flow(DCF) model, and option thinking DCF model with general DCF model. Real Options, especially 6-folded option tree model yields boner estimate of value than values using other methods. According to sensitivity analysis, sales of final products, number of investigational new drug developments(INDs) and success rates of each stage are key factors for the value of biotechnology R&D investment.

• Proceedings of the Korea Electromagnetic Engineering Society Conference
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• 2000.11a
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• pp.385-388
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• 2000

본 논문에서는 좀더 효율적이고 소형화한 GPS(Global Positioning System)용 안테나와 LNA(Low Noise Amplifier)를 IMT-2000 단말기에 내장함으로써 개인 휴대 통신 기능과 더불어 좀 더 정확한 위치추적 기능을 동시에 가능케 하고자 한다. 중심 주파수 1.575 GHz의 저잡음 증폭기와 안테나의 크기는, 합쳐서 10$\times$10$\times$4 (mm)로서 상층은 마이크로스트립 패치 안테나이고, 중간층은 안테나 ground와 LNA ground의 공통 ground이며, 제일 아래층에는 LNA가 위치하게 된다. LNA 의 경우 2단을 중첩하여, 첫째 단 16dB, 둘째 단 18dB의 이득 특성을 보였는데 첫째, 둘째 단의 대역통과 필터에서 삽입손실로 3dB의 손실을 가져와 총 3dB의 이득 특성을 보였다. 잡음 특성은 약 1.9의 특성을 보였다. 안테나의 경우 9$\times$9$\times$$\times$2 (mm)로써, 고유전율( $\varepsilon$$_{r}$ = 73 )의 세라믹을 사용하여 그 크기를 상당부분 줄였다. 그리고 유전체 밑의 ground를 옆면까지 높임으로써 좀 더 소형화한 안테나를 가능케 하였다. 고유전율의 유전체를 사용하였기에 안테나 자체의 이득 특성은 저잡음 증폭기에서 보상하고, 안테나의 임피던스 정합 또한 LNA의 입력 쪽에서 하도록 하였다. 또한 위성신호 수신을 위해 안테나는 RHCP 의 원형편파 특성을 갖는다.

• Journal of Genetic Medicine
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• v.4 no.2
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• pp.160-166
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• 2007

Purpose : The ${\beta}$-adrenoceptors are pharmacologically classified into ${\beta}_1$-, ${\beta}_2$- and ${\beta}_3$-adrenoceptor. The gene of each subtype has polymorphisms related to their function (${\beta}_1$-adrenoceptor: Ser49Gly, ${\beta}_2$-adrenoceptor: Gln27Glu, ${\beta}_3$-adrenoceptor: Trp64Arg). The objectives of this study were to analyse the allelic and genotypic distribution of the representative polymorphism of ${\beta}$-adrenoceptors in preeclampsia and to investigate whether combined genotype of ${\beta}$-adrenoceptors may be associated with preeclampsia. Methods : Blood samples were collected from a Korean population (159 preeclamptic pregnancies and 168 normotensive pregnancies). The ${\beta}_1$-, ${\beta}_2$- and ${\beta}_3$-adrenoceptor genotypes was determined using polymerase chain reaction-restriction fragment length polymorphism. Results : There were no differences in allelic and genotypic distribution of ${\beta}_1$- and ${\beta}_2$-adrenoceptor polymorphisms between the two groups. However, the Arg allele of ${\beta}_3$-adrenoceptor polymorphism were more frequent in preecalmpsia than in controls (P<0.05, OR=1.57, 95% CI=1.01-2.46). Moreover, prevalence of genotype carrying heterozygote of ${\beta}_3$-adrenoceptor polymorphism was increased in preeclampsia compared with controls (P<0.05, OR 1.76, 95% CI 1.06-2.92). When combination of the three polymorphisms were evaluated, pregnancies with the particular combined genotype that is consisted of heterozygote of ${\beta}_1$-, ${\beta}_3$-adrenoceptor and wild homozygote of ${\beta}_2$-adrenoceptor (Ser/Gly, Gln/Gln, Trp/Arg), showed a significant increase in the risk of preeclampsia (P<0.05, OR=3.01, 95% CI 1.12-8.08). Conclusion : A particular combined genotype (Ser/Gly, Gln/Gln, Trp/Arg) of - adrenoceptors was associated with the risk of preeclampsia.

• Proceedings of the International Microelectronics And Packaging Society Conference
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• 2001.11a
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• pp.29-35
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• 2001

Combline 구조를 가진 마이크로 스트립 라인 또는 스트립 라인 필터를 내장한 기능형 PCB를 제작하였다. 저 비용 구현 및 이동성과 휴대성을 강조하기 위해 기존의 세라믹 대신 FR4와 에폭시를 유전체로 사용하는 저가형 다층 회로 기판의 도체 층에 집적화 하였다. Combline의 각 끝단에 커패시터를 부하함으로써 전기적 길이를 확장하였고 전체적인 크기를 감소시킴으로서 적당한 필터 특성을 얻을 수 있었다. 구현된 Embedded PCB는 마이크로파 대역에서 사용 가능하며 특히 Bluetooth 나 Wireless LAN과 같은 ISM 대역을 사용하는 무선통신소자로서 사용 가능하다.

• Proceedings of the Ginseng society Conference
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• 2004.05a
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• pp.17-28
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• 2004

As Korean ginseng is hybrid, an individual variation is very severe, and it takes long times in new breeding because it is required 4 years to pick the seed. But, transformation technique makes the high-functional breeding in short time. The focus of these ginseng studies is to find and secure the useful gene. And it is urgent to accumulate the fundamental data for the molecular breeding and secure the useful genes. Therefore, transformation and soil acclimatization technique are necessary to molecular breeding in use of the introduction of functional genes. In this study, it add to secure of new regulation gene and useful gene as to accumulate the fundamental data for the place where it will contribute to raise the national competitive power. To analyze the useful genes in large scale, we constructed CDNA libraries with various tissues, species, and treated tissue. EST analysis of ginseng perform in large scale and build the EST database of ginseng. We perform the full length sequencing about the selected lots of clones that include the entire open reading frame of the amino acid residues and construct cDNA chip with the parental EST clones. Establishment of the transformation and a soil acclimatization system throuth the re-introduction of the selected ginseng gene that related with the secondary metabolism and anti-stress into the ginseng.

• Proceedings of the Korean Information Science Society Conference
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• 2003.04d
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• pp.157-159
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• 2003

BioPlace는 유전체 연구자에게 유전체 검색 시스템을 제공하고 특정 연구 분야의 연구자들 사이에 연구 관련 정보를 효율적으로 공유하고 교환하기 위한 웹기반 협업지원 환경이다. BioPlace 시스템에서 효과적인 공동작업을 수행하기 위해서는 개인 및 작업그룹간의 일정을 편리하게 관리할 수 있는 일정관리 도구가 필요하다. 본 논문에서는 BioPlace 시스템 상에서 개인 및 작업그룹의 일정을 편리하고 효율적으로 관리할 수 있는 도구를 JSP 및 EJB 컴포넌트 기술을 이용하여 개발하였다. 개발된 일정관리 컴포넌트는 일정보기, 일정추가, 일정수정, 일정삭제, 공지전달 등의 기능을 통하여 사용자들 간의 효율적인 공동작업을 수행할 수 있는 편리함을 제공한다.

• Korean Journal of Human Ecology
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• v.22 no.1
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• pp.181-188
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• 2013

노인에게서 두드러지게 나타나고 있는 저근육형 비만은 근육감소를 동반한 체지방의 증가로 신체상의 뚜렷한 체성분의 변화를 야기 시킨다. 이때 골감소증을 동반하여 신체기능의 감소 및 골절장애 그리고 대사성 관련 질환의 위험도가 올라가는 것으로 보고되고 있다. 노화로 인한 체성분의 변화는 단순한 저근육형일 경우와 비만일 때 보다 급격히 증가된 복부내장 지방조직에서 분비되는 염증성 사이토카인, C-반응성 단백질(CRP), 인터루킨(IL)-6, IL-8 및 종양 괴사 인자(TNF-${\alpha}$)들이 단백질 대사를 저해하여 근육량의 감소를 더욱 촉진시키며, 염증관련 대사질환의 유병률에 중요한 요인이다. 본 연구에서는 DNA 메틸화가 당뇨병, 심혈관질환, 암과 같은 만성염증성 질환에 관계하고 있다는 최근 연구 결과를 기초로 하여 항염증 영양소와 생리활성을 갖는 식품인자들의 충분한 섭취가 염증조절에 중요하게 기여할 것으로 생각되며, 또한 염증성 질환의 주요 표식자인 DNA 메틸화와 히스톤 변형을 유발하는 효소의 활성 또는 비 암호화된 RNA의 발현을 조절함으로써 근육량 증가와 체지방 감소에 중요한 역할을 하는 것을 살펴보았다. 따라서 최근 새롭게 인식되는 후생유전학적 연구의 중심에 있는 항염증 영양소의 효과와 체성분 변화와의 긍정적 관계를 중심으로 저근육형 비만의 예방 및 인구고령화에 건강한 노화를 위한 효과적인 방법을 제시하였다.

• Journal of Life Science
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• v.32 no.10
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• pp.771-777
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• 2022

Dopaminergic (DA) cell death in Parkinson's disease (PD) has been attributed to multiple, distinct genetic and environmental factors. In rare familial PD loss of parkin function mutations play a key role in nigral DA neuron-specific pathogenesis primarily via endoplasmic reticulum (ER) stress. In more prevalent sporadic PD, environmental exposure to pesticides has a significant epidemiological role. However, it is largely unknown how environmental exposure to xenobiotics is etiologically linked with the known etiology in familial PD. In the present study biochemical evidence for a common pathogenic mechanism between sporadic and familial PD has been identified employing the recently characterized mesencephalic DA cell line, N27-A. Dieldrin, an organochlorine pesticide epidemiologically implicated in sporadic PD, induced the markers of ER stress response such as a chaperone BiP/Grp78, heme oxygenase-1 and especially, parkin. Accordingly, dieldrin activated the ER resident Caspase-12, a mediator of ER stress-specific apoptosis, during cell death of N27-A cells. Of great interest the dieldrin-induced DA neuronal cell death was synergistically rescued by the overexpression of ER resident neuroprotective proteins, parkin and Bcl-2. The present findings implicate that accumulation of ER stress could be one of common pathogenic mechanisms in idiopathic and familial PD, and some ER proteins, such as parkin and Bcl-2 may effectively attenuate ER stress-mediated N27-A DA cell death.