• The Korean Journal of Zoology
• /
• v.27 no.3
• /
• pp.151-164
• /
• 1984

In order to investigate the mechanism of myoblast fusion during muscle differentiation in culture, the effect of muscle-conditioned medium on the fusion was studied and possible release from cultured myoblast cells of proteins which may be responsible for the promotion of myoblast fusion was analyzed. The muscle-conditioned medium showed a marked fusion-promoting activity in a dose-dependent fashion. THis fusion-promoting activity of the muscle-conditioned medium appeared to be due to the accumulation of at least two proteins which were released from the myoblast into the culture medium. These released proteins were analyzed by electrophoresis and autoradiography and found to have molecular weights of 45,000 and 65,000.

• The Journal of the Petrological Society of Korea
• /
• v.14 no.4 s.42
• /
• pp.226-236
• /
• 2005

Using the concept of granite suite/supersuite we hierarchically divided the Triassic granites in South Korea which have spatio-temporally close relationships each other. Among the Triassic granites in the Okcheon belt (western Yeongnam massif), the Baegrok granite and the Jeomchon granite can be grouped into one suite, the Baegrok suite, whereas the Cheongsan granite into the Cheongsan suite. These two suites can be grouped again into a larger supersuite, the Baegrok supersuite, on the basis of the similarity in the source rocks and the contrasts in the petrographic and geochemical characteristics. Three Triassic granites in the Gyeongsang basin - the Yeongdeok granite, the Yeonghae granite, and the Cheongsong granite - can be grouped into the Yeongdeok suite, Yeonghae suite and Cheongsong suite, respectively. These three suites can be grouped again into a larger supersuite, the Yeongdeok supersuite, on the basis of the similarity in the source rocks and the contrasts in the petrographic and geochemical characteristics. Nd-Sr isotopic signatures for the Baegrok supersuite are quite distinct from those for the Yeongdeok supersuite, indicating that the source materials of each granitic magma were not identical. The source rocks for the Baegrok supersuite are thought to be a mixture of two crustal components of the Yeongnam massif, whereas those for the Yeongdeok supersuite to be a mixture of the depleted mantle with the crustal components of the Yeongnam massif. The fact that the two contemporaneous granite supersuites were derived from the different sources can be explained by the difference of the tectonic environments where the granitic magmas were produced.

• The Korean Journal of Zoology
• /
• v.38 no.4
• /
• pp.483-489
• /
• 1995

Retinoic acid was found to block membrane fusion of chick embryonic myoblasts in culture. This effed was dosedependent and could he reversed upon removal of the agent from the culture medium. Furthermore, the retinoic acid-mediated inhibition of membrane fusion was observed with the fusion competent cells but not with the cells that had already been committed for fusion, indicating that the effect of RA is differentiation stage-specific. However, retinoic acid showed little or no effect on the ability of the cells to form bipolar shape and to align along their axes. Neither the cell proliferation nor accumulation of muscle specific proteins, such as creatine kinase and tropomyosin, was impaired significantly. On the other hand, retinoic acid blocked the differentiation time~ependent loss of fibronectin, whose process is prerequisite for myoblast fusion. These results suggest that retinoic add acts as a specific inhibitor of membrane fusion by preventing the loss of fibronectin from the differentiating myoblasts.

• The Korean Journal of Zoology
• /
• v.27 no.2
• /
• pp.73-84
• /
• 1984

In order to investigate the effect of neurotransmitter on myoblast differentiation in vitro, the effects of dopamine and epinephrine on myoblast fusion and on the intracellular cAMP level in cultured myoblasts were examined. Dopamine $(3\\times10^{-5}M)$ and epinephrine $(3\\times10^{-5}M)$, when added at 34 hr after cell plating, markedly inhibited myoblast fusion, and dopamine was more potent than epinephrine. Both dopamine and epinephrine had no effect on intracellular cAMP level. At the same time, exogeneous dbcAMP, $PGE_1$, and aspirin were used to examine whether cAMP is involved in myoblast differentiation. dbcAMP $(1\\times10^{-4}M)$ inhibited myoblast fusion, whereas $PGE_1 (3\\times10^{-6}M)$ had no inhibitory effect, rather enhancing myoblast fusion. Aspirin, an inhibitor of PG synthetase, was shown to inhibit myoblast fusion. Possible mechanism by which dopamine or epinephrine at a specific concentration used inhibits myoblast fusion is discussed.

• 월간산업보건
• /
• s.326
• /
• pp.31-35
• /
• 2015

• 월간산업보건
• /
• s.289
• /
• pp.56-57
• /
• 2012

• 월간산업보건
• /
• s.336
• /
• pp.26-29
• /
• 2016

• Proceedings of the Korea Institute of Fire Science and Engineering Conference
• /
• 2002.11a
• /
• pp.288-292
• /
• 2002

• Korean Journal of Food Science and Technology
• /
• v.6 no.2
• /
• pp.79-85
• /
• 1974

To obtain further information concerning the nature myofibrillar proteins in a food system, an investigation has been conducted to compare the change in the biochemical property of the myofibril with the changes in the morphological structure of the myofibril. When myofibrils were prepared with 0.16 M KCl-0.04 M Tris-HCl, the band pattern was clear and distinct. There was a uniform thickening of A-band, a sharp appearence of Z-lines and a wide I-band. The band pattern of myofibrils was changed as the composition of extraction solution was changed. Also the ATPase activity of myofibril changed as the length of sarcomere changed. When myofibrils were treated with a low concentration of trypsin, myofibrils turned in the contracted state. With the progress of prolonged trypsin treatment, most of myofibrils exhibited a pattern of alternating light and dark bands, supercontracted pattern. Although myofibrils exhibited a supercontracted band pattern, the ATPase activity of myofibril continued to increase with the progress of trypsin treatment. An assumption was made that tropomyosin may be located in Z-line and that troponin-tropomyosin complex can inhibit the ATPase activity of myofibrils through the structural alternation of myofibril.

• Proceedings of the Korea Contents Association Conference
• /
• 2019.05a
• /
• pp.119-120
• /
• 2019

이 글은 물질의 본질로 규정한 아리스토텔레스의 색채론과 디지털이미지의 비물질적 특성으로서의 검정을 전제로, <신과 함께>의 서사를 주도하는 인물과 이미지를 구성하는 공간에 채워진 검정의 의미를 분석한다. 할리우드영화에서 검정은 악의 근원이며 공포이다. 검정에 관한 예외적이고 특별한 특성을 성찰하는 철학, 회화, 문학에서의 비유와 달리, 대중영화에서 검정은 가장 보편적인 의미를 표현한다. 삶과 죽음의 경계를 오가는 <신과 함께>에서 검정은 저승의 공간과 차사라는 인물을 구성한다. 한국영화에서 검정은 상상의 공간을 상징화하는 유용한 색이다. 이 글은 한국영화 <신과 함께>에 사용된 검정을 통해 동서양 색채문화의 차이를 살펴보고, 사회문화적 맥락에 따라 상호작용하는 의미를 분석한다.