• Journal of Life Science
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• v.21 no.9
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• pp.1281-1287
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• 2011

Phyllostachys consists of high and fast growing trees and is a genus in the family Gramineae. The genus has many species in Asia, with main distribution being in India and China. One of the most popular sequences for phylogenetic inference at the generic and infrageneric levels in plants is the internal transcribed spacer (ITS) region of the 18S-5.8S-26S nuclear ribosomal cistron. We evaluated four taxa with the ITS region to estimate phenotypic relationships within the genus Phyllostachys in Korea. Alignment of the DNA sequences required the addition of numerous gaps. Sequence variation within the Phyllostachys was mostly due to natural selection, although several indels and inserts were found. Within the genus Phyllostachys, P. nigra and P. nigra var. henonis were the relatives in the three phylogenetic analyses (MP, ML, and NJ). However, some external nodes were poorly supported. Morphological traits and simple repeats (ISSR) represented the result of a relationship similar to the that of ITS sequences in the genus Phyllostachys. This suggests that ITS sequences are very informative for identification of these taxa.

• Korean Journal of Ichthyology
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• v.35 no.3
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• pp.151-159
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• 2023

This study investigated the early life history of the Micropercops swinhonis in Botongcheon stream Hangang River and compared the relationship between the related species and flexibility. The fertilized egg of the ovoid type, and the average egg diameter was 1.23 mm in long diameter and 0.85 mm in short diameter. At a water temperature of 20.6℃, the hatching time took 179 hours. The newly hatched larvae measured 3.82~4.08 mm in total length (TL), and their mouth and anus were not yet open. At 18 days after hatching, the postflexion larvae reached 7.97~8.43 mm in TL, with the tip of the caudal fin bent at 45°. At 29 days after hatching, the juveniles reached 9.83~10.8 mm in TL and were differentiated by their constant number of fins (7~10 first dorsal, 10~11 second dorsal, 7~10 anal fins). As a result of the study, there was a difference from the related species in the egg diameter, the newly hatched larva size. The postflexion larvae period and the development period of the first dorsal fin were the slowest among the comparable simila species, and the size was characterized by a large number, indicating a difference from similar species in the early life history process.

• Korean Journal of Plant Resources
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• v.29 no.5
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• pp.547-554
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• 2016

The genus Tilia is characterized by linear form bracts of which the lower part is attached to the peduncle of a cyme. This character is distinguished from the others genus of Malvaceae. The purpose of this study is verifying the phylogenetic relationship of genus Tilia. Phylogenetic analyses were conducted to evaluate relationships of 10 taxa of Tilia in Korea and Japan including one outgroup (Gossypium hirsutum). The molecular phylogenetic analyses were conducted with sequences based on ITS, trnL-F and rpl32-trnL region. The combined data result of ITS, trnL-F and rpl32-trnL was formed by 6 clades. T. kiusiana situated as the most basal clade. T. amurensis, T. taquetii and T. rufa are composed a clade. T. koreana, T. insularis and T. japonica was formed independent clade. T. insularis has the closest relationship with T. japonica. T. miqueliana, T. mandshurica, and T. megaphylla are composed a clade and showed a sister relationship than other species.

• Applied Microscopy
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• v.40 no.4
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• pp.219-228
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• 2010

The seeds of 16 species from sect. Anthacantha and related taxa were examined using light and scanning electron microscopy to illucidate the boundary of the section and their relationship among species. Using six qualitative characters clustering analyses were conducted, and three types were recognized. Type I including species from sect. Anthacantha+sect. Meleuphorbia is characterized by the ovate shape, rounded at base and smooth along the ventral line. Type II including species from sect. Medusae+sect. Treisia-1 is squared in shape and are tuberculate. Tubercles are prominent along the ventral line. Type III (sect. Treisia-2 group) is mostly rounded, and as in type II, tubercles are prominent along the ventral line, but the unique crestae consisting of the grouping testa cells differed from the surface patterns of Types I and II. Based on the seed morphology, sects. Anthacantha and Meleuphorbia are closely related, which is well supported by the results from molecular and pollen morphological studies. Besides, sects. Treisia and Medusae are not closely related in terms of seed characters, and this is not consistent with the results of recent molecular studies.

• The Korean Journal of Soil Zoology
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• v.8 no.1_2
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• pp.7-12
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• 2003

Endogeneous endoglucanase (EC 3.2.1.4) cDNA was cloned from a representative species (Eisenia anderi) of the earthworm family Lumbricidae. Endoglucanase from the midgut of the earthworm is composed of 456 amino acids and belongs to glycosyl hydrolase family 9 (GHF9), sharing high homologies (50-51 %) with those of selected termite and crayfish. This endoglucanase consists of three consensus catalytic domains found in most microbial cellulases. A phylogenetic tree was constructed using the amino acid squence data matched through the BLASTX program and showed that GHF9 families could be divided into four groups of arthropoda, bacteria, plant and annelida.

• Korean Journal Plant Pathology
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• v.12 no.1
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• pp.91-98
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• 1996

벼도열병균 14개 균주와 벼 이외 화본과 식물 도열병균 12개 균주를 대상으로 rDNA의 ITS II 부위를 증폭하여 그들의 핵산 구조 차이를 분석함으로 도열병균 균주간 분류를 시도하였다. 5.8S rDNA의 3`-말단 부위와 28S rDNA의 5`-말단 부위의 sequence 중 5`-CCCGGGAATTCGCATCGATCGATCGAATGAAGA-ACGCAGC-3`와 5`-CCCGGGATCCTCCGCTTATT-GATATGC-3`를 이용하여 PCR 증폭을 하였을 때 벼도열병균 14개 균주는 동일한 길이의 단일 밴드를 형성하였으며 벼 이외 화본과 식물 도열병균에서는 레드톱 식물로부터 분리한 도열병균만이 나머지 균주보다 38bp가 더 큰 길이를 가진 밴드를 형성하였다. PCR로 증폭된 DNA를 HaeIII와 MspI 제한효소로 절단하였을 때 벼도열병균 레이스간에는 제한효소 절단에 의한 전기영동 밴드 형태 차이를 관찰할 수 없었으나, 벼 이외 화본과 식물 도열병균 12개 균주는 3군으로 구분할 수 있었다. 벼도열병균 90=054와 강아지풀에서 분리한 도열병균 G90-5, 기장에서 분리한 G88-4, 바랭이에서 분리한 G88-5 그리고 레드톱에서 분리한 RT 균주의 ITS II 부위의 DNA 염기서열 비교 분석에 의하면 G88-4와는 다른 HaeIII와 MspI 제한효소 위치를 가지고 있었기에 제한효소 절단에 의한 전기영동 형태가 상이하였다. 또한 RT균주는 HaeIII와 MspI위치가 존재하지 않았다.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2005.05a
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• pp.199-202
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• 2005

본 연구는 cytochrome B 유전자의 PCR-RFLP 분석기법을 이용하여 다양한 가금류 식육자원 및 각종 가공 육제품의 원료육에 대한 정확하고 재현성 높은 축종 및 육종 감별기술을 개발하기 위하여 수행되었다. 국내에서 유통되고 있는 종간 근연관계가 높은 3종의 가금육(닭, 칠면조 및 오리)의 육류에서 분리한 DNA 시료를 대상으로 cytochrome B 유전자의 특정 염기서열을 포함하는 primer를 설계 제작하여 PCR-RFLP 분석을 실시하였다. 각 축종의 근육조직으로부터 genomic DNA를 추출하고 PCR 증폭 반응을 수행한 후 얻어진 PCR 증폭산물(359 bp)을 두 종류의 제한효소(HaeШ 및 Hinf I)로 각각 절단한 결과 특히, HaeШ 제한효소에서 가금류의 축종 간 그리고 제한효소 간에 명확한 차이를 보이는 종 특이적인 PCR-RFLP marker를 검출하였다. 따라서, 본 연구에서 개발한 cytochrome B 유전자의 가금류 종 특이적 PCR-RFLP marker는 가금류의 식육 및 가공 육제품의 육종 및 축종 판별에 매우 유용한 DNA marker로 이용될 수 있을 것이다.

• Animal Systematics, Evolution and Diversity
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• v.6 no.2
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• pp.235-250
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• 1990

This study deals with 8 species in 6 genera including 5 new species and 2 new records from Taiwan. The new ones are Neanura kentingensis n.sp., Womersleya formosana n.sp., Crossodinthina montana n.sp., Vitronura tubercula sp., Lobella nana n.sp., and 2 new records are Pseudachorucina nepalica Yosii, 1966 and Lobella perfusa Denis., 1934. The Neanuridae fauna of Taiwan accordingly is listed as 13 species in 7 genera. The biogeographic affinities with the Japan, Vietnam and adjacent countries are discussed.

• The Korean Journal of Zoology
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• v.32 no.4
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• pp.393-403
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• 1989

Using starch gel electrophoresis of proteins, we examined variation at 24 genetic loci in five species (three general of the Subfamily Danioninae (Class Pisces, Family Cyprinidae) inhabiting In Korea. The average degree of genic variation of the Subfamily Danioninae (A=1.8, P=16.5%, HD=0.043, HG=0.049) was far less than that of the fresh water fishes in general. But the degree of genic variation of A. chinensis (P=32.5 HD=0.100, HG=0.119) was highe, than other fishes and the value of O. biders was the lowest (P=4.2%, HD=0.008, HG=0.008). The average genetic similarities among the populations in each species of A. chinensis, Z. platypus, Z. temmincki MS type, Z. temmincki W type, and O. widens were S=0.902, 0.899, 0.990, 0.914, and 0.992, respectively. The average genetic similarity of A. chinensis to the other species was the lowest value of S=0.235, whereas that between two species of Z. temmincki was the highest of S=0.822. Genetically, Z. plypus is more similiar to O. widens than ho species of the same genus Zacco. Based on estimated divergent time of 5 species of the Subfamily Danioninae (7.6 to 0.9 million years) it is assumed that they are speciated during late Pliocene to middle Pleistocene epoch.

• Korean Journal of Medicinal Crop Science
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• v.8 no.3
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• pp.243-249
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• 2000

Analysis of random amplified polymorphic DNAs(RAPDs) and internal morphological features were performed using three species of medicinal plants in the genus of Angelica(A. gigas Nakai, A. sinensis(Oliv.) Diels., A. acutiloba Kitagawa) to distinguish between these three species. Fifty decarmer oligonucleotide primers were screened for the RAPDs of the herbal plant species. Five primers generated distinct RAPD markers specific to the species of Angelica, In analysis of the degree of similarity, A. sinensis(Oliv.) Diels is more closely related to A. acutiloba Kitagawa than to A. gigas Nakai. Furthermore, we proved the usefulness of RAPD analysis for the discrimination of the species using dry roots and commercial plant materials. In internal morphology of three species, A. sinensis(Oliv.) Diels seemed to be more specialized in systemic than A. acutiloba Kitagawa and A. gigas Nakai