• Korean Journal of Veterinary Research
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• v.42 no.2
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• pp.191-199
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• 2002

In this study, eight primers were used to detect genetic variability and phylogenetic relationships among the eighteen Salmonella strains by the arbitrary-primed PCR(AP-PCR) techniques. Five strains of Salmonella typhimurium, four strains of S entertidis, three strains of S choleraeuis, three strains of S gallinarum and three strains of S pullorum were typed by AP-PCR. The number of AP-PCR bands detected per each primer varied from 39 to 52, with an average of 43.6. A total of 349 AP-PCR bands were generated and among them, 185 bands(53.0%) were polymorphic. Among the primers, GEN 703 and GEN 708 primer showed a high level of polymorphism with 0.682 and 0.676, respectively. But GEN 603, GEN 604 and GEN 607 primer showed a low level of polymorphism with 0.404, 0.460 and 0.472, respectively. Therefore, the these primers will be the most effective for AP-PCR analysis of Salmonella strains. The level of polymorphism of S typhimurium CU 2001(0.77) was similar to that of S typhimurium CU 2002(0.77) and lower than those of other strains such as S typhimurium CU 2003(0.63), S typhimurium ATCC 14028(0.50) and S typhimurium CU 2004(0.43). The level of polymorphism of S enteritidis ATCC 13076(0.83) was similar to that of S enteritidis CU 2005(0.83) and lower than those of other strains such as S enteritidis CU 2006(0.63) and S enteritidis CU 2007(0.58). The level of polymorphism of S choleraeuis CU 2009(0.67) was similar to that of S choleraeuis CU 2010(0.67) and higher than those of other strains such as S choleraeuis CU 2008(0.53). The level of polymorphism of S gallinarum CU 2011(0.70) was similar to that of S gallinarum CU 2012(0.70) and higher than those of other strains Such as S gallinarum ATCC 9184(0.60). The level of polymorphism of S pullorum CU 2013(0.80) was similar to that of S pullorum CU 2014(0.80) and higher than those of other strains such as S pullorum No 11(0.53). Therefore, the AP-PCR analysis will be used a powerful tool for estimating genetic variation and phylogenetic relationships among Salmonella strains.

• Horticultural Science & Technology
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• v.17 no.2
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• pp.141-143
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• 1999

Genetic relationship of Calanthe discolor, C. sieboldii, and C. bicolor for elucidating the classification was investigated. Electrophoretic zymograms for either peroxidase or esterase isozymes indicated that bands of C. bicolor appear in the zone where those of C. discolor and C. sieboldii are located. Genetic relationship among the three Calanthe species using RAPD analysis showed that C. discolor and C. sieboldii are more distant each other than C. bicolor, demonstrating the genetic position of C. bicolor between the other two. It was assumed that C. bicolor is a natural hybrid between C. discolor and C. sieboldii.

• Proceedings of the Zoological Society Korea Conference
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• 1994.10a
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• pp.94.1-94
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• 1994

• Proceedings of the Zoological Society Korea Conference
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• 1994.10a
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• pp.93.1-93
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• 1994

• Proceedings of the Zoological Society Korea Conference
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• 2002.04a
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• pp.11-12
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• 2002

No Abstract, See Full Text

• Animal Systematics, Evolution and Diversity
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• v.11 no.2
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• pp.235-242
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• 1995

The nucleotide sequence variation of mitochondria1 DNA were investigated by eight restriction endonucleases from two oyster species, Crassostrea gigas collected from two localities of South Korea and one locality of Japan and C. nippona collected from one locality of South Korea. The total mtDNA size in the oyster, C. gigas, from the three localities was approximately 18 kb and that in C. nippona was 22 kb. The restriction fragment patterns of mtDNA in C. gigas from the three localities by BamHI, BgII, and XhoI digestions were identical to one another. The degree of mtDNA sequence divergence of C. gigas between the two localities in Korea was 2% and that between Korean and Japanese C. gigas was 5%. The amount of sequence divergence between the two species of oysters was 42%.

• Applied Biological Chemistry
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• v.36 no.3
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• pp.139-145
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• 1993

The methods of isoelectric focusing of 4 isozymes in polyacrylamide horizontal slab gels and restriction fragment length polymorphisms (RFLPs) were applied to characterize the biochemical phenotypes of 19 cultivars of barley. Among 19 barley cultivars screened, 7 esterase, 3 phosphoglucose isomerase, 4 peroxidase and 2 alcohol dehydrogenase isozyme phenotypes were distinguished by isoelectric focusing. When purified DNA of each cultivar was digested with restriction enzyme EcoRV and analyzed its RFLPs with barley DNA markers pMSU 51 or pMSU 71, two distinct RFLP patterns were shown. Based on the four isozymes and two RELP polymorphisms, 19 cultivars of barley were classified into 13 biochemical phenotypes. Phylogenetical relationships among 13 biochemical phenotypes classified were determined using Nei's F-statistics and the phylogenetic dendrogram was constructed.

• Korean Journal of Environment and Ecology
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• v.11 no.3
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• pp.277-289
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• 1997

Electrophresis methods were used to examined the degree of genetic relatedness and divergent times on the 4 genera 4 species of the family Corvidae and 1 species of the Family Cinclidae. Genetic relatedness between two families and between 4 genera of Corvidae showed similar to those reported at comparable taxonomic levels in other bird. But it appears to be considerably less than that of non avian taxa. Genetic relatedness between Garrulus glandarius brandtii and Corvus corone orientalis was the most close related(S=0.81), whereas Pica pica sericea(S=0.72) and Cyanopica cyanus koreensis(S=0.68) were showed less close relatedness respectively. The presumed divergent time between two families was 3.9 million years before present. Whereas among the 4 species of corvidae, the presumes divergent times of Cyanopica cyanus koreensis and Pica pica sericea were about 1.9 and 1.6 million years before present respectively, and between Garrulus glandarius brandtii and Corvus corone orientalis was showed recently about 1.0 million years before present

• The Korean Journal of Malacology
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• v.9 no.1
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• pp.39-45
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• 1993

한국산 부산입술대고둥의 지역 집단간에 있어 유전적 변이, 종내 유연관계를 밝히기 위하여 애월, 세화, 삼척, 통도사, 거문도 및 여수 지역에서 채집한 총 180개체를 대상으로 전기영동을 실시하였고 여기서 나타난 16개의 유전자를 분석하였다. 각 집단의 유전전 변이 정도를 조사한 결과 전 집단의 평균 대립인자 수 (A)는 1.528이고, 평균 다형형의 빈도(P)는 45.5%이며, 평균이형접합자(H$_{D}$)는 0.205로 나타났다. 이중 삼척집단의 대립인자 수는 1.46으로 가장 낮고, 애월집단의 다형형의 빈도는 28.58%, 통도사의집단은 57.14%로 가장 낮은 빈도를 나타냈다. 각 집단간 유전적 유연관계를 분석한 결과 6개 집단간의 평균 유전적 근연치는 S=0.716이었고, 애월과 삼척집단은 0.903으로 가장 가까운 유전적 유연관계를 4보여주고 있다. 여수집단과 삼척집단의 유전적 유연관계는 0.562로 가장 낮은 값을 나타냈다. Nei의 공식에 따라 각 집단의 유전적 차이치(D)를 계산한 결과 평균D값은 0.267이며, 이중 애월과 삼척집단이 0.067로 가장 가깝게 나타났고 이는 Roger에 의한 유전적 근연관계와 유사한 양상을 보였다.

• Animal Systematics, Evolution and Diversity
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• v.13 no.3
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• pp.233-246
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• 1997

A genetic analysis using starch gel electrophoresis was performed to clarify the degrees of genetic differentiation and the phylogenetic relationships among four species of the genus Corbicula (Bivalvia, Corbiculidae). The average genetic similarity coefficients among the populations of each species on C. fluminea, C. leana and C. colorata were very closed (Rogers' S$\geq$0.970), whereas the other C. japonica was separated into two genetic groups (S=0.873) with distinct allelic difference at the Gpl locus. Thuefore additional taxonomic studies are needed on this species. On the other hand, these four species were well distinguished from each other genetically with distinct allelic differences at 4 to 9 loci. The genetic similarity coefficients (S) between C. leana and C. colorata, C. fluminea and C. leana, and C. fluminea and C. colorata were 0.737, 0.689, and 0.594, respectively. In particular, the average genetic similarity coefficients between these three species (inhabiting in freshwater) and the other C. japonica (inhabiting in brackishwater) were notably lower (S=0.370).