• Title/Summary/Keyword: 균핵병

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채소작물 병해방제 집중탐구 (5)호박 - 점차 발생이 늘고 있는 문제 병해들 - 잿빛곰팡이병$\cdot$균핵병$\cdot$검은별무늬병$\cdot$세균병해$\cdot$모자이크병

  • 김충회
    • The Bimonthly Magazine for Agrochemicals and Plant Protection
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    • v.13 no.2 s.107
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    • pp.76-83
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    • 1992
  • 호박은 비교적 병해가 적은 작물로 알려지고 있지만 사실은 호박의 재배면적이 일반화되지 않아서 그런 느낌이 들 뿐이지 시설재배단지에서는 어느 작물 못지 않게 병해의 발생이 심하다. 우리나라에서 호박에 발생하는 병해는 모두 12가지가 알려지고 있다. 그러나 호박재배에서 가장 큰 골칫거리 병해는 흰가루병, 노균병, 잿빛곰팡이병, 균핵병, 검은별무늬병, 세균성반점병, 바이러스병등 수종에 불과하다. 이외에 역병, 덩굴마름병, 탄저병, 무름병등이 발생하고 있으나 위에 열거한 병에 비하여 일반적이지 못하며 피해도 크지 않다.

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Occurrence of Sclerotinia Rot by Sclerotinia minor on Aster yomena in Korea (한국에서 Sclerotinia minor에 의한 쑥부쟁이 균핵병 발생)

  • Lee, Sang Yeob;Choi, Hyo-Won;Weon, Hang Yeon;Han, Ji Hee;Kim, Dayeon;Ahn, Sungho
    • The Korean Journal of Mycology
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    • v.46 no.2
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    • pp.200-204
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    • 2018
  • Sclerotinia rot symptoms were frequently found on the stems of Aster yomena in the Gurye region of Korea in April 2016. The symptom, watery soft rot, mainly appeared on the stems, and severely infected plants blighted. White mycelia spread over the stems of the infected plants and the soil surface. Small black sclerotia formed on the plant lesions and inside the diseased stems. Incidence of the disease was as high as 20~80% in the A. yomena fields. Based on the morphological and molecular characteristics of the isolates, the fungi were identified as Sclerotinia minor. This is the first report of Sclerotinia rot caused by Sclerotinia minor on A. yomena in Korea.

Effect of Temperature on Sclerotia Formation and Viability of Sclerotinia sclerotiorum Causing Sclerotiorum Rot of Cryptotaenia japonica (파드득나물 균핵병균(Sclerotinia sclerotiorum)의 균핵 헝성 및 생존에 미치는 온도의 영향)

  • Chang, Seog-Won;Lee, Han-Bum;Kim, Sung-Kee
    • Research in Plant Disease
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    • v.9 no.1
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    • pp.47-51
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    • 2003
  • Sclerotinia rot of C. japonica caused by Sclerotinia sclerotiorum was observed throught the growing season in the vinylhouse at the Namyangiu, Yangju and Yangpyung areas. The disease was commonly occurred at late autumn after transplanting and continuously increased until spring in next year, The disease was especially severe at the Yangpyung area among three areas. Three isolates of S. sclerotiorum obtained from C. japonica in different geographic areas were evaluated for ability of sclerotia formation and viability under different temperature. Temperature for mycelial growth ranged from 5 to 3$0^{\circ}C$, with optimum temperature at 15~3$0^{\circ}C$. Sclerotia were fewer formed at low temperature, but their size was larger than that at high temperature. Small size of sclerotia was more viable than those of large size at high temperature. Based on the differences in viability of sclerotia, the relative ranks of sclerotia were in order ~2 mm > 3~4 mm > 5 mm~.

Selection of Bacillus amyloliquefaciens M27 for Biocontrol on Lettuce Sclerotinia Rot (상추균핵병의 생물적 방제를 위한 Bacillus amyloliquefaciens M27 선발)

  • Lee, Sang Yeob;Weon, Hang Yeon;Kim, Wan Gyu;Kim, Jeong Jun;Han, Ji Hee
    • The Korean Journal of Mycology
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    • v.43 no.3
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    • pp.180-184
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    • 2015
  • For selection of effective antagonists for control Sclerotinia rot of lettuce, 29 bacteria were isolated from soil in Korea. The bacterial isolates M27 and RM43 identified as Bacillus sp, were selected as prospective agents for inhibiting mycelial growth of Sclerotinia sclerotiorum and Sclerotinia minor. Among the selected isolates, Bacillus amyloliquefaciens M27 effectively suppressed incidence of Sclerotinia rot by Sclerotinia sclerotiorum and Sclerotinia minor in the lettuce nursery.

Growth Inhibition of Sclerotium Cepivorum Causing Allium White Rot by Serratia plymuthica Producing Chitinase (Serratia plymuthica AL-1이 생산하는 chitinase에 의한 대파 흑색썩음균핵병균의 생육억제)

  • 김진호;최용화;강상재;김영훈;주길재
    • Journal of Life Science
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    • v.13 no.1
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    • pp.90-98
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    • 2003
  • An allium rhizobacterium Serratia plymuthica AL-1 was previously selected as a biocontrol agent of allium white rot. The chitinase from S. plymuthica AL-1 produced in medium containing colloidal chitin was purified by ammonium sulfate precipitation (40~70%), affinity adsorption, column chromatography on DEAE-sephadex A-50 and sephadex C-200 gel filtration. The enzyme was purified 10.8-fold with a yield of 7.3% from the starting culture broth. The purified chtinase gave a single band on sodium dodecyl sulfate polyacrylamide gel electrophoresis, it's molecular weight was estimated to be 55 kDa. The optimum pH and temperature of the purified enzyme were pH 5.5 and $55^{\circ}C$, respectively and it is stable up to $50^{\circ}C$ and maintains around 90% of its activity for 60min. The enzyme were activated by $Ca^{2+}$, $Mn^{2+}$ and $Mg^{2+}$ and inhibited by $Cu^{2+}$, SDS, $\rho$-CMB, MIA, respectively. The purified chitinase showed broad spectrum of antifungal activities against plant pathogenic fungi Sclerotium cepivoruin, Alternana alternnta, Colletotrichum glceosporioidrs, Phoma sp., Sclerotinia sclerotiorum, Stemphylium solani, Fusarium oxysporium f. sp. niveum but rarely inhibited Phytophthora capsici and Pythium ultimum.. The purified chitinase from S. plymuthica AL-1 caused swelling, lysis, deceleration and degradation of the hyphal tips of S. sczerotiorum causing allium white rot. It suggest that S. prymuthica AL-1 chitinase play an important part in the bifunctional chitinase / lysozyme activity.

Ecological Studies on Lettuce Drop Disease Occurring under Controlled Cultivation Conditions in Drained Paddy Fields (답리작 상치 시설재배지에서의 균핵병 발생생태에 관한 연구)

  • Shin Dong Bum;Lee Joon Tak
    • Korean Journal Plant Pathology
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    • v.3 no.4
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    • pp.252-260
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    • 1987
  • Incidence of lettuce drop was observed throughout the growing season in the vinylhouse at the southern part of Korea, Kimhai. Occurrence of this disease was especially severe at the seedling stage. Number of sclerotia in surface soil $(30\times30\times5cm)$ was 22.0 at the seedling stage, and 5.3 at harvest in the infected area. Temperature for mycelial growth ranged from 5 to $30^{\circ}C$ with optimum temperature at $25^{\circ}C$. Sclerotia were formed fewer at low temperature, but their size was larger resulting in heavier dry weight than that at high temperature. The apothecia were formed from the sclerotia that were buried in March, April and September upto 3cm soil depth, but formed from those buried only 1 em soil depth in October. Sclerotia buried in June and December did not form apothecia regardless of soil depth by 90 days. The sclerotia buried in the 5cm of soil depth did not form apothecia. Sclerotia that were embedded in wet or flooded soil at $25^{\circ}C$ and $30^{\circ}C$ for 5 weeks lost their viability. Infection of lettuce was possible with mycelia originated from sclerotia on autoclaved lettuce plant fragments. The fungus was pathogenic on 25 plant species in 8 families in artificial inoculation tests. Lettuce seedlings appeared to be infected by airborne ascospore originated from sclerotia on crops and weeds around paddy fields, because sclerotia existing in soil might perish under long flood conditions during rice cultivation.

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Biological Control of White Rot in Garlic Using Burkholderia pyrrocinia CAB08106-4 (Burkholderia pyrrocinia CAB08106-4 균주를 이용한 마늘 흑색썩음균핵병의 생물학적 방제)

  • Han, Kwang Seop;Kim, Buyng Ryun;Kim, Jong Tae;Hahm, Soo Sang;Hong, Ki Heung;Chung, Chang Kook;Nam, Yun Gyu;Yu, Seung Hun;Choi, Jae Eul
    • Research in Plant Disease
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    • v.19 no.1
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    • pp.21-24
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    • 2013
  • White rot caused by Sclerotium cepivorum was reported to be severe soil-born disease on garlic. Disease progress of white rot of garlic (Allium sativum L.) was investigated during the growing season of 2009 to 2011 at Taean and Seosan areas. The white rot disease on bulb began to occur from late April and peaked in late May. The antifungal bacteria, Burkholderia pyrrocinia CAB08106-4 was tested in field bioassay for suppression of white rot disease. As a result of the nucleotide sequence of the gene 16S rRNA, CAB008106-4 strain used in this study has been identified as B. pyrrocinia. B. pyrrocinia CAB080106-4 isolate suppressed the white rot with 69.6% control efficacy in field test. These results suggested that B. pyrrocinia CAB08106-4 isolate could be an effective biological control agent against white rot of garlic.