• Korean Journal of Microbiology
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• v.47 no.4
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• pp.328-334
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• 2011

Leucine zipper motif consists of multiple periodic leucine residues, which forms amphipathic alpha helix. The hydrophobic nature of leucine zipper motif can dimerize proteins which contain this motif. Leucine zipper motif addition at C-terminus of single-chain Fv (ScFv) antibody induces its dimerization. Since the dimeric ScFv antibody contains two antigen binding sites (bivalency) like Y-shaped complete antibody, it could increase avidity. As a result, it could show higher antigen binding activity than monomeric ScFv antibodies. Based on this concept, monomeric chicken 8C3 ScFv antibody previously developed from chicken hybridoma was dimerized by the addition of leucine zipper motif at C-terminus of ScFv antibody. The dimeric 8C3 ScFv antibody specifically reacted with Eimerian sporozoite which causes Avian Coccidiosis. As expected, dimeric 8C3 ScFv antibody showed 3-folds higher antigen binding activity than monomer due to increased avidity. In addition, protien yields of dimer expression were 2-folds higher than monomer.

• Korean Journal of Organic Agriculture
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• v.19 no.3
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• pp.343-355
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• 2011

This work was studied the effects of spore density and infection of arbuscular mycorrhizal fungi (AMF) for no-tillage organic cultivation of pepper with wintering green manure crops cultivation in greenhouse field. Spore density of arbuscular mycorrhizal fungi (AMF) in green manure crops was 189 spores/30g fresh soils in control including alive spore (82 spores). Spore density of AMF in all green manure crops was totally 196~226 spores/30g fresh soil and alive spore was 84~112 spores/30g fresh soil. Spore density of AMF in soils of Pepper crop was range of 48.0~56.7 spores/30g fresh soils after cultivation of green manure crops. Infection structure of AMF was not significantly difference in soils of green manure crops and Pepper crop after cultivation of green manure crops. Infection rate of AMF in roots of green crops was low level by 2.8% in giant chickweed, 7.4% in rye, 9.3% in hairy vetch. Infection rate of AMF in roots of barley was the highest level by 20.3%. Infection rate of AMF in roots of Pepper crop was range of 5.2~7.2% after cultivation of green manure crops Also, infection rate of AMF in roots of Pepper crop was 8.1% after the harvest of barley. Infection structure of AMF in barley very well consisted of network with internal hyphae, while hairy vetch and rye tended to no network. There was not a significant relationship between spore density in soils and infection rate of AMF in rhizosphere of Pepper.

• Korean Journal of Ecology and Environment
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• v.49 no.4
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• pp.308-319
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• 2016

In order to evaluate the role of macrophytes as refuge of zooplankton on physical distribution (i.e. summer rainfall), we investigated the environmental factors, macrophytes, and zooplankton in waterside zones (macrophytes zones) and open water zones of 17 wetlands from May and August, 2011. In this study, a total of 51 zooplankton species were identified, and Polyarthra sp. and Diaphanosoma brachyurum were found to be the most dominant species. Waterside area of each wetland were occupied by a total of 10 macrophyte species, species composition and biomass (dry weight) were different in the survey sites. Zooplankton was more abundant in waterside zone than open water zones lacking macrophytes (One-way ANOVA, df=2, F=27.1, P<0.05), in particular, waterside zone of 1, 8, 9, 10, and 11 wetland were supported by high zooplankton density after summer rainfall. This wetlands were developed by various macrophyte species than other wetland, and submerged plant commonly presented. Waterside zones with various macrophyte species provides complexity to the habitat structure, should be utilized as refuge to avoid disturbance such as summer rainfall. The results indicate that macrophytes are the key components to enhance bio-diversity include zooplankton, and the inclusion of diverse plant species in wetland construction or restoration schemes will result in ecologically healthy food webs.

• Korean Journal of Food Science and Technology
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• v.40 no.2
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• pp.129-134
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• 2008

Five antioxidative active substances were isolated from the EtOAc layer (20 g/56.2 g) of Korean black raspberry (Rubus coreanus Miquel) wine (11 L, black raspberry 15.7 kg fresh wt. eq.) by various column chromatography and high performance liquid chromatography (HPLC). Proton nuclear magnetic resonance ($^1H$-NMR) spectroscopy and gas chromatography Electro Ionization-Mass Spectrometry (GC-EI-MS) identified these as 4-hydroxybenzoic acid (1, 0.1 mg), 3,4-dihydroxybenzoic acid (2, 0.3 mg), 4-(2-hydroxyethyl)-phenol (3, 0.6 mg; tyrosol), pyrocatechol (4, 0.3 mg), 3,4,5-trihydroxybenzoic acid ethyl ester (5, 0.6 mg; ethyl gallate). The presence of 1 and 2 in Korean black raspberry has previously been reported. However, the presence of 3-5 in Korean black raspberry, and the identification of 1-5 from the Korean black raspberry wine have never before been reported.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2001.04b
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• pp.75-76
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• 2001

순환식 펄라이트재배에서 배액 재사용을 위한 양분흡수 모델링을 작성하고자 EC 처리(1.5, 1.8, 2.1, 2.4, 2.7 dSㆍm-1)를 수행하였다. 생육 중기까지 EC 수준에 따른 양액흡수량은 차이가 없었지만 중기 이후 EC가 높을수록 흡수량이 감소되는 경항을 보였다(Fig. 1). NO$_3$-N, P 및 K의 흡수량은 생육기간 동안 처리간 차이를 유지하였는데 N과 K는 생육 중기 이후 일정 수준을 유지하였으나 P는 생육기간 동안 다소 증가되는 경향을 보였다. S의 흡수량은 생육 중기 이후 모든 처리에서 급격한 감소를 보였으며 생육 후기에는 처리간에 차이가 없었다(Fig. 2). 오이의 무기이온 흡수율에서와 같이 흡수량에서도 EC간 차이를 보여 EC를 무기이온 흡수량을 추정하는 요소로 이용할 수 있을 것으로 생각되었다. 무기이온 흡수량은 모든 EC 처리간에 생육 초기에는 차이를 보이지 않았으나 생육중기 이후에는 뚜렷한 차이를 보인 후 생육 후기의 높은 농도에서 그 차이가 다소 감소되는 경향을 보였다. 단위일사량에 따른 양액흡수량과 EC를 주된 변수로 한 오이의 이온 흡수량 예측 회귀식을 작성하였는데 모든 무기이온 흡수량 추정식의 상관계수는 S를 제외한 모든 이온에서 높게 나타났는데 특히 N, P, K 및 Ca에서 높았다. S이온에서의 상관계수는 0.47로 낮게 나타났으나 각 이온들의 회귀식에 대한 상관계수는 모두 1% 수준에서 유의성을 보여 위의 모델식을 순환식 양액재배에서 무기이온 추정식으로 사용이 가능할 것으로 생각되었다(Table 1). 이를 이용한 실측치와의 비교는 신뢰구간 1%내에서 높은 정의상관을 보여 실제적인 적용이 가능할 것으로 생각되었다(Fig 3)..ble 3D)를 바탕으로 MPEG-4 시스템의 특징들을 수용하여 구성되고 BIFS와 일대일로 대응된다. 반면에 XMT-0는 멀티미디어 문서를 웹문서로 표현하는 SMIL 2.0 을 그 기반으로 하였기에 MPEG-4 시스템의 특징보다는 컨텐츠를 저작하는 제작자의 초점에 맞추어 개발된 형태이다. XMT를 이용하여 컨텐츠를 저작하기 위해서는 사용자 인터페이스를 통해 입력되는 저작 정보들을 손쉽게 저장하고 조작할 수 있으며, 또한 XMT 파일 형태로 출력하기 위한 API 가 필요하다. 이에, 본 논문에서는 XMT 형태의 중간 자료형으로의 저장 및 조작을 위하여 XML 에서 표준 인터페이스로 사용하고 있는 DOM(Document Object Model)을 기반으로 하여 XMT 문법에 적합하게 API를 정의하였으며, 또한, XMT 파일을 생성하기 위한 API를 구현하였다. 본 논문에서 제공된 API는 객체기반 제작/편집 도구에 응용되어 다양한 멀티미디어 컨텐츠 제작에 사용되었다.x factorization (NMF), generative topographic mapping (GTM)의 구조와 학습 및 추론알고리즘을소개하고 이를 DNA칩 데이터 분석 평가 대회인 CAMDA-2000과 CAMDA-2001에서 사용된cancer diagnosis 문제와 gene-drug dependency analysis 문제에 적용한 결과를 살펴본다.0$\mu$M이 적당하며, 초기배발달을 유기할 때의 효과적인 cysteamine의 농도는 25~50$\mu$M인 것으로 판단된다.N)A(N)/N을 제시하였다(A(N)=N에 대한 A값). 위의 실험식을 사용하여 헝가리산 Zempleni 시료(15%

• Journal of KIISE:Computer Systems and Theory
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• v.29 no.4
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• pp.201-212
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• 2002

This paper proposes a reconfigurable scheduler model that can support various real-time scheduling algorithms. The proposed model consists of two hierarchical upper and lower components, task scheduler and scheduling framework, respectively. The scheduling framework provides a job dispatcher and software timers. The task scheduler implements an appropriate scheduling algorithm, which supports a specific real-time application, based on the scheduling framework. If system developers observe internal kernel interfaces to communicate between two hierarchical components, they can implement a new scheduling algorithm independent of complex low kernel mechanism. Once a task scheduler is developed, it can be reused in a new real-time system in future. In Real-Time Linux (5), we implemented the proposed scheduling framework and several representative real-time scheduling algorithms. Throughout these implementations, we confirmed that a new scheduling algorithm could be developed independently without updates of complex low kernel modules. In order to confirm efficiency of the proposed model, we measured the performance of representative task schedulers. The results showed that the scheduling overhead of proposed model, which has two separated components, is similar to that of a classic monolithic kernel scheduler.

• Resources Recycling
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• v.6 no.3
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• pp.15-21
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• 1997

Flyash from a coal-hed power plan1 is produced approximtcly 3 million tons m 1996 and causes the serious environmentalpmblem due to the disposal in the ash pond. Flyash is an accepted additive in concrete where it adds strength, sulfate ateresistanceand reduced cost, provided acccptablc levcls of unbunrned carbon are mmtmed This papzr describes to investigate thc technicalfeasibility of a dry triboelcctrostatlcp roccss to scparate unburned carbon h m f lyash into economically valuable produck Puliclesof unburned carbon and flyash can be impded positivc and negative surface charzes. rcapeclively. with a copper tniochargcr dueto dirferences in the work function values of thc particles and the tnbacharger. and cm he separated by passing thcm throuph anexternal electic field. A laboratory s d e separation system consists of r sacw feeder for ash supply, a tniocharger, verticalcollecling copper plates, power supplies, a flow meter, and a fan. Separation tests taking into account separahian efficiency and ashrecovery showed that flyash recovery was sh-nngly dependent an thc tnbocharger geomzhy, elect"c ficld strength. flyssh s ~ c a,n dash feeding late. Optimal separation conditions were flyash size less than 125 Fm and electric field shcngrh of 200 kV1m. Ovcr 80%of the flyash with 7% lass on ignition was recovered at wrbon contznts less than 3%bon contznts less than 3%

• Journal of the Korean Institute of Intelligent Systems
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• v.27 no.1
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• pp.1-6
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• 2017

Recent researches on radio frequency energy harvesting networks(RF-EHNs) with limited energy resource like battery have been focusing on the development of a new scheme that can effectively extend the whole lifetime of a network to semipermanent. In order for considerable increase both in the amount of energy obtained from radio frequency energy harvesting and its charging effectiveness, it is very important to design a network that supports energy harvesting and data transfer simultaneously with the full consideration of various characteristics affecting the performance of a RF-EHN. In this paper, we proposes an interference-based charging aware routing protocol(ICARP) that utilizes interference information and charging time to maximize the amount of energy harvesting and to minimize the end-to-end delay from a source to the given destination node. To accomplish the research objectives, this paper gives a design of ICARP adopting new network metrics such as interference information and charging time to minimize end-to-end delay in energy harvesting wireless networks. The proposed method enables a RF-EHN to reduce the number of packet losses and retransmissions significantly for better energy consumption. Finally, simulation results show that the network performance in the aspects of packet transmission rate and end-to-end delay has enhanced with the comparison of existing routing protocols.

• Review of Culture and Economy
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• v.20 no.2
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• pp.89-117
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• 2017

As the industrial paradigm shifts and the manufacturing industry declines, many changes also take place in the region as well. In this regard, interest in industrial heritage as a facet of cultural heritage is on the increase. In this paper, the meaning of regional 'cultural contents' as industrial heritage is investigated within the scope of specific region. It is meant to move beyond the viewpoint of considering industrial heritage as only relating to industrial machinery and relevant landmarks from the past. The concept of industrial heritage is established more clearly through the review policy and case study analysis of existing research; the analysis is conducted to investigate the characteristics associated with it, and then to explore how best to utilize it. In particular, this paper attempts to focus on how it operates within these parameters using a spatio-temporal context as much as possible, and concentrating on the recognition and experience of the subject of industrial heritage as being traceable through human story. This research is based on the case of 'Sapporo' which focuses on modern history based on historical importance, and the 'Cheongju' case study, which contrasts the former by focusing on urban regeneration using a spatial lens. This paper identifies the possibility of regional development through the examination of past identity and diversity in the present, and highlights the features that could be linked to future usability and development. In addition, it proposes the possibility that the cycle of regional development could change in the process of the different stages of territorialization, de-territorialization and re-territorialization.

• Korean Journal of Comparative Education
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• v.26 no.4
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• pp.81-103
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• 2016

The reform of teacher policies to raise the quality of teachers has been one of the major priorities of countries around the world. Especially, the structure of elementary and secondary teacher education institutions has been the focus of international teacher policy reform agenda. Despite the problems faced by Korean teacher education system, the reform policy effort has not been actively pursued. Based on this problem recognition, this study seeks to analyze the case of Ireland to reveal the characteristics of teacher education system restructuring including background of restructuring, key strategies, and major contents. We found that the restructuring of Irish system was triggered by both domestic and international initiatives to tackle the problems of the system. The restructuring effort was led by the government and showed several distinctive features, such as reducing the number of institutions, combining elementary and secondary teacher education institutions, and strengthening research capacity of institutions. Base on the findings, a few policy recommendations were made to reignite the discussions on the reform of teacher education system in South Korea.