• Title/Summary/Keyword: 구성아미노산

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Analysis on the Components of the Angelica dahurica Root (구릿대(Angelica dahurica) 뿌리의 성분 분석)

  • Joo, Eun-Yong;Kang, Won-Ja
    • Food Science and Preservation
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    • v.12 no.5
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    • pp.476-481
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    • 2005
  • This study was carried out to analyze the components of the Angelica dahurica root for the studies of the physiological function. General components of A. dahurica root were 71.7% moisture, 10.6% carbohydrate, 9.3% crude protein, 6.6% crude ash and 1.9% crude fat. The content of reducing sugar was 1,850 mg/100 g. The total amount of free sugar was 80 mg/100 g, in which 19.3 mg/100 g fructose, 27.8 mg/100 g glucose, 28.4 mg/100 g sucrose and 4.5 mg/100 g maltose were present. In the results of mineral analysis, the content of K was the highest(2,145.03 mg/100 g) followed by 286.35 mg/100 g Mg and 145.23 mg/100 g Ca. The total amount of hydrolyzed amino acid was 71.68 mg/100 g, in which 20.98 mg/100 g essential amino acid and 50.70 mg/100 g of non-essential amino acid were present. Among them, proline(11.74 mg/100 g) was the highest. Total free amino acids were contained 17.04 mg/100 g, in which 6.67 mg/100 g of essential amino acids and 10.37 mg/100 g of non-essential amino acids were present. Among them alanine(5.96 mg/100 g) was the highest. Total content of amino acid derivatives was 3.37 mg/100 g.

Coloring Behavior of Using Color Reactions of Wool-Tryptophan and Aldehyde Derivative (양모섬유의 기능발색 -양모의 트립토판과 알데히드 유도체의 발색반응에 의한 염색-)

  • 김경필;김혜인;박수민
    • Proceedings of the Korean Fiber Society Conference
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    • 2003.04a
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    • pp.198-201
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    • 2003
  • 양모와 견과 같은 동물성 섬유는 주성분이 단백질로 구성되어 있으며 그 기본성분은 아미노산이다. 단백질섬유는 모두 동물에서 형성된 생체고분자의 일종으로서 우리 인간의 세포조성물질과 유사하여 가장 친화성이 있는 섬유라고 할 수 있다. $^{1)}$ 아미노산을 구성물질로 하는 이들의 섬유 중에는 -NH2 등의 염기성기, -COOH 등의 산성기, 그 외 -SH 등을 가지고 있으므로 광범위한 염료가 염착한다. 즉, 산성, 크롬착염, 반응, 직접, 염기성, 그리고 배트염료 등이 적용가능하다. (중략)

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Proximate, Sugar and Amino Acid Compositions of Dolsan Leaf Mustard(Brassica juncea) (돌산 갓의 일반성분, 당 및 아미노산 조성)

  • 조영숙;박석규;전순실;문주석;하봉석
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.22 no.1
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    • pp.48-52
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    • 1993
  • To furnish basic data for the utilization of leaf mustard as a raw material of salted and fermented vegetable food, major chemical compositions of Dolsan Leaf Mustard (DLM) were investigated. The moisture and ash contents of DLM were 87.5% and 1.4%, respectively. Compared with other Korean traditional or Japanese leaf mustard, DIM contained more crude protein (3.8%) but less crude fat (0.3%) and crude fiber (1.3%). As an alkali food, leaf and leat stalk had pH of 5.7 and 5.8, titratable acidity of 687 and 318mg/100g and alkalinity of 2.5 and 5.2, respectively. The major free sugar in DLM was glucose and fructose was also detected in a small amount. The contents of total and reducing sugar in leaf and leaf stalk were 574, 352, 820 and 538mg%, respectively. Total amino acid contents of leaf and leaf stalk were 8.0 and 2.5% on wet basis. Although the amino acid compositions of leaf and leaf stalk were different each other, glutamic acid and proline were the major amino acids in both of the leaf and leaf stalk. Significantly higher amount (ca.10.3-fold) of free amino acid was present in leaf (3074mg%) than in leaf stalk (298mg%). Glutamic acid and aspartic acid were the major free amino acids and essential amino acid contents in leaf and leaf stalk were 869 and 68mg% being 22.9 and 28.3% of total free amino acids.

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Studies on the Amino Acid and Fatty Acid Compositions in the Seed and Pulpy Substance of Feral Peach (Prunus persica Batsch var. davidiana Max.) (야생 돌복숭아 씨와 과육의 아미노산 및 지방산 조성에 관한 연구)

  • Kim, Han-Soo
    • Journal of Life Science
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    • v.17 no.1 s.81
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    • pp.125-131
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    • 2007
  • Amino acid and fatty acid compositions of the physiological activity substance in the seed and pulpy substance of feral peach (Prunus persica Batsch var. davidiana Max.) were analyzed for the use as an biohealth functional processed products. The proximate compositions in the vacuum freeze dried seed and pulpy substance of feral peach were carbohydrate 63.92% and 75.11%, crude protein 27.85% and 12.77%, moisture 3.61% and 4.69%, crude fat 1.21% and 4.80%, crude ash 3.41% and 2.63%, respectively. Total amino acid contents in the protein of feral peach seed were 3,444.35 mg%, and the major amino acids were aspartic acid(681.10 mg%), glutamic acid(495.48 mg%), alanine(283.66 mg%), serine(251.36 mg%), proline(229.80 mg%), lysine(192.31 mg%) and leucine(191.34 mg%), respectively. Total amino acid contents in the protein of feral peach pulpy substance were 1,064.02 mg%, and the major amino acids followed aspartic acid(250.15 mg%), glutamic acid(129. 63 mg%), lysine, proline, leucine, alanine and serine, in a decreasing order. The richest total amino acid content contained in feral peach seed and pulpy substance was aspartic acid, followed by glutamic acid. The amount of free amino acids of feral peach seed were 6,215.34 ms%, and the major free amino acids were glutamic acid(827.25 mg%), threonine, valine and $\beta-aminobutyric$ acid, respectively. Free amino acid contents of pulpy substance were 683.82 mg%, and the major free amino acids were glutamic acid(339.49 mg%), serine proline, alanine and $\gamma-amino-n-butyric$ acid. Especially, in the case of glutamic acid, it was highest. The compositions of major total fatty acid in the lipid feral peach (Prunus persica Batsch var. davidiana Max.) seed and pulpy sabstance were linoleic acid($C_{18:2}$, n-6) and linolenic acid($C_{18:3}$, n-3), particularly.

Analysis on the Components of the Vitex rotundifolia Fruit and Stem (순비기나무(Vitex rotundifolia) 열매와 줄기의 함유성분 분석)

  • Lee, Yang-Suk;Joo, Eun-Young;Kim, Nam-Woo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.2
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    • pp.184-189
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    • 2008
  • This study was conducted to analyze components of the fruit and stem of Vitex rotundifolia as part of a study on the nutritional and functional materials, and the development of a functional food. General components of V. rotundifolia fruit are moisture 12.92%, carbohydrate 78.67%, crude protein 3.22%, crude fat 1.73% and 3.46% crude ash. V. rotundifolia stem was moisture 11.30%, carbohydrate 80.87%, crude protein 4.78%, crude fat 0.64 % and 2.41% crude ash. The content of V. rotundifolia reducing sugar was 646.07 mg% (fruit) and 1,547.97 mg% (stem). The total amount of free sugar was fruit 5.66 mg% and stem 90.79 mg%. The content of soluble protein was 3,268.12 mg% in fruit and 4,927.55 mg% in stem. The polyphenol compound content was 608.06 mg% and 808.06 mg%, respectively. Total amount of hydrolyzed amino acid of V. rotundifolia fruit and stem were 3,095.75 mg% and 2,135.84 mg%, while that of free amino acid of fruit and stem were 79.99 mg%, 81.20 mg%, among which cysteine (2,010.82 mg%) was the highest in the V. rotundifolia fruit. In the results of mineral analysis, the content of K was the highest in fruit (2,184.00 mg%) and stem (1,469.20 mg%).

Food Components and Volatile Flavors in Rhodiola sachalinensis Roots (홍경천(Rhodiola sachazinensis) 뿌리의 식품학적 성분 및 휘발성 향기성분)

  • 이은정;임지순;박채규;전병선;김석창
    • Food Industry And Nutrition
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    • v.9 no.1
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    • pp.53-57
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    • 2004
  • 홍경천(Rhodiola sachalinensis)의 성분을 분석하여 식품학적 특성을 검토하고 식품소재로서의 기초자료로 활용할수 있도록 건조된 홍경천을 분쇄, 분말화한 후 일반성분 및 무기성분, 유리당, 구성아미노산, 유리지방산, 그리고 향기성분을 분석하였다. 홍경천 건조분말의 수분은 10.14%, 조단백 3.90%, 조지방 1.33%, 총당 41.17%, 환원당 11.40% 회분은 3.05%로 분석되었고, 무기성분은 Ca, K, Kg이 높은 함량으로 나타났다. 유리당으로는 glucose, fructose, sucrose 등으로 특히 glucose와 fructose가 높게 함유되어있었다. 주된 구성아미노산은 glutmic acid, cystine arginine, proline, aspartic acid, isoleucine, histidine 등의 함량 순으로 나타났으며, 특히 glutamic acid, cystine, arginine 함량이 각각 176.24, 168.60, 159.90 mg%로 높은 함량 수준을 보였다. 유리지방산은 linoleic acid가 32.46%로 함량이 가장 높았으며 그 다음으로 palmitic acid가 21.42%, lignoceric acid 14.83%, oleic acid 13.09% behenic acid가 5.80% 순으로 나타났다. 향기성분은 GC-MS로 분석한 결과 2-cinnamic aldehyde 함량이 가장 높았으며 geraniol, myrtenol, octanol 등의 함량이 높게 나타났다. 이러한 결과로 볼 때 홍경천 뿌리는 다양한 일반성분 조성과, 필수아미노산, 불포화지방산, 필수 무기질 등이 균형있게 함유된 식품학적 성분 가치가 충분한 소재임이 확인되었다. 따라서 향후 홍경천 뿌리에서 유효성분을 추출하여 생리활성 및 동물실험을 통해 이들 식품학적 성분들의 유용성을 탐색하는 작업이 필요하리라 사료된다.

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Studies on the Plant Pathogenic Corynebacteria(III) -The amino acid composition of some plant pathogenic bacteria- (Corynebacterium 속 식물병원세균에 관한 연구(III) - 식물병원세균의 아미노산 조성)

  • Kim Jong-wan;Mukoo Hideo
    • Korean journal of applied entomology
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    • v.14 no.4 s.25
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    • pp.209-213
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    • 1975
  • This paper is a report of studies to determine the amino acid composition of Plant Pathogenic Corynebacteria and to assess whether these characteristics can be correlated with the taxonomic position of these organisms. The results indicated that plant pathogenic Corynebacteria contained on average less cystein, trypthophane, histidine, phenylalanine, isoleucin and total protein than did the other genera of bacteria. However, in general, the quantities of both cell protein and amino acids contained in bacterial cells were characteristics of the species or an individual strain of the organism and were not related to its classification.

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Content Analyses of Fiber, Protein and Amino Acids of Fully Ripe Fruits of Korea Native Squash, Cucurbita moschata Poir (한국재래종 호박 완숙과의 섬유질, 단백질 및 아미노산 함량 비교분석)

  • Youn, Sun-Joo;Jun, Ha-Joon;Kang, Sun-Chul
    • Applied Biological Chemistry
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    • v.47 no.4
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    • pp.403-408
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    • 2004
  • We studied active substances like crude cell wall components, crude protein, composing amino acids and free amino acids including orinithine cycle-related amino acids such as asparagine, ornithine and citrullin in fully ripe fruits of Korean native squash, Cucurbita moschata Poir. Crude protein content of 'Jeju 2' was the highest with $2,830\;{\mu}g/g$, while 'Sangju' was the lowest with $1,319\;{\mu}g/g$. Regarding the contents of crude cell wall components, fruit 'Kanghaw' was the highest with 2,961 mg% while 'Namhea' was the lowest with 1,582 mg%. Pectin contents of crude cell wall components were the highest in 'Kanghaw' (2,198 mg%) followed by 'Jeju 2' (2,178 mg%) and 'Jeju l' (1,461 mg%). The main contents of amino acids in squash were glutamic acid, aspartic acid, lysine, leucine and valine, which comprised to be more than 50% of total amino acid contents. Especially, in 'Jeju 2' aspartic acid and threonine were not detected. In fully ripe fruits, a total of 34 kinds of free amino acids were detected including 8 kinds of essential amino acids (histidine, isoleucine, leucine, lysine, phenylalanine, methionine, threonine and valine). More than 50% of the total free amino acids were aspartic acid and asparagine, and also all varieties were detected in ornithine, citrullin, and arginine, which are related to Ornithine cycle. There was a big difference in the contents of arginine in all varieties whereas the contents of ornithine and citrullin were very similar. 'Teaan' 29.34% was 7 times higher than 'Namhea' 4.30% in regards to arginine contents.

Purification and Characterizating of Recombinant Human Albumin from Hansenula polymorpha DL-1 (Hansenula polymorpha DL-1이 생산하는 재조합 알부민의 정제 및 특성)

  • 최근범;구선향;임채양;이동희;강현아;이상기
    • Microbiology and Biotechnology Letters
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    • v.29 no.4
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    • pp.248-252
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    • 2001
  • Recombinant Human serum albumin (rHSA) was purified to near homogeneity from H, polymorpha using heat treatment, ultrafiltratipn and Phenyl Sepharose CL-4B and Mono Q column chro - matographies with a recovery yield of 60% The molecular weight of the purified rHSA was estimated to be about 65,000 Da by denaturing SDS-PAGE The N terminal amino acid sequence of the purified HSA determined by Edman degradation was turned out to be Asp- Ala- His- Lys- Ser- Glu- Ala, suggesting that the rHSA expressed in H, polymorpha was efficiently secreted and correctly processed at the cleavage site of secretion signal sequence. The purified human albumin showed the pI value identical to that of authentic human serum albumin.

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An Algorithm for Computing Valid Side Chain Conformations for Finding Transformed Channels in a Protein Molecule (단백질 분자에서 변형된 채널 발견을 위한 유효 사이드 체인 배치 알고리즘)

  • Choi, Jihoon;Kim, Byungjoo;Kim, Ku-Jin
    • KIPS Transactions on Computer and Communication Systems
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    • v.4 no.1
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    • pp.1-4
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    • 2015
  • This paper presents an algorithm for finding valid side chain conformations of amino acids, when given channel is transformed. The suggested algorithm implements a protein molecule with flexible side chains based on the flexibility of amino acids, and extracts adjacent amino acids that affect the formation of the channel. We detect the collision between adjacent amino acids and neighbors, in order to exclude invalid side chain conformations. Then, we construct the rotation angle combination tree to choose valid side chain conformations.