• Horticultural Science & Technology
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• v.34 no.1
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• pp.154-162
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• 2016

The aim of this study was to develop a transgenic petunia with enhanced resistance to sulfur dioxide ($SO_2$) gas by stacking two genes, SOD2 and NDPK2, which are both known to confer resistance to abiotic stresses. The first-generation hybrids ($TF_1$) were obtained through reciprocal crosses between an SOD2-transgenic line SOD2-2-1-1-35($T_4$)[S($T_4$)] and an NDPK2-transgenic line NDPK2-7-1($T_2$)[N7-1($T_2$)]. Approximately 32.1-73.0% of the first-generation hybrids ($TF_1$) carried both SOD2 and NDPK2 genes. These hybrids showed 2.6 and 5.1 times less damage than hybrids carrying only SOD2 or NDPK2 genes, respectively, when they were treated with $SO_2$ gas at 30 ppm. This confirmed that the heterozygous hybrids were more resistant to $SO_2$ than the hybrids carrying either one of the resistance genes. Second-generation hybrids ($TF_2$) were obtained by selfing the $TF_1$ individuals. We confirmed the expression of the stacked genes in the $TF_2$ hybrids by phenotypic observation of their response to $SO_2$ gas at 30 ppm as well as using RT-qPCR and photosynthetic efficiency.

• Korean Journal of Ichthyology
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• v.5 no.1
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• pp.113-121
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• 1993

Cytogenetic studies and hybridization experiment using two populations of Oryzias latipes from Korea were conducted in order to examine their systematic positions. In the chromosome number of specimens examined. O. sinensis had 46 with a pair of large metacentrics and O. latipes had 48 chromosomes without a pair of large metacentrics. However, the diploid chromosome number of the hybrid between O. latipes and O. sinensis was 47 and they had a large metarcentric chromosome in their karyotype. In the $F_2$ hybrids, distribution of chromosome number was variable among individuals. Hatching rates showed little difference between control and hybrids. However, hatchiabilties of $F_2$ hybrids were decreased by 83.3% as compared with $F_1$ offspring. Incidence of abnormal larvae was increased by 15.3% as compared with their parents. These results indicate that the two karyotype populations formerly regarded as two subspecies would be two distinct allopatric sibling species O. sinensis, and O. latipes.

• Proceedings of the Korean Society of Crop Science Conference
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• 1980.05a
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• pp.22.2-22.2
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• 1980

• KOREAN JOURNAL OF CROP SCIENCE
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• v.23 no.2
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• pp.34-39
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• 1978

Partial dominance was exhibited by flowering(heading), panicle length, panicle numbers, $F_1$ag leaf length, 1000 kernel weight in $F_1$ and $F_2$ hybrids by 7 \times 7 and 5 \times 5 diallel crosses. Over dominance was exhibited by culm length, flag leaf width, appearance degree of panicle in $F_1$ generation of the crosses, and also over dominance was exhibited by kernel weight in $F_2$ generation of the crosses.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.3
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• pp.287-293
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• 1985

Degree of heterosis and gene distributions of several agronomic characters of rice were studied with F$_1$ and F$_2$ generations of 9-parent partial diallel cross in 1983-1984. Degree of heterosis was greater in F$_1$ hybrids than F$_2$ hybrids for culm length, panicle length and number of panicle and the heterobeltiosis could be observed in culm length and number of panicle from several crosses. Incomplete dominance was exhibited by culm length, panicle length and number of panicle in F$_1$ and F$_2$ generations.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.23 no.2
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• pp.25-33
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• 1978

7 parent varieties and 21 $_{x}\textrm{F}_1 and $_{x}\textrm{F}_2 hybrids from 7 X 7 partial diallel crosses were used as the materials, and degree of heterosis was studied in 9 agronomic characters of rice. Degree of heterosis was more remarkable in $_{x}\textrm{F}_1 hybrids than $_{x}\textrm{F}_2 hybrids. and significant differences were showed in many hybrid generations. and also over dominance could be shoo wed in eight characters except 1000 kernel weight of rice.

• Journal of Plant Biotechnology
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• v.31 no.4
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• pp.261-266
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• 2004

This experiment was carried out to confirm the stability of bar gene introduced into petunia plant through Agrobacterium-mediated transformation, in successive generation, or after crossing or back-crossing. Some of different 25 transgenic plants were used in crossing and back-crossing to wild type, or repeated-selfing to T$_4$ generation. On the processing of experiment, it was found that some lines lost their resistant ability to herbicide basta, or showed non-Mendelian segregation mode: produced much more susceptible segregants than resistant plants. Even though there are exceptional cases, which was off from expected, the genetic stability of bar gene introduced could be confirmed strongly, because in almost case, the segregation of resistant and susceptible plants to basta was done under Mendelian-law according to single gene dominant model.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.20
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• pp.1-26
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• 1975

In order to provide basic information for the application of tissue culture to a rice breeding program. experiments were carried out on the callus formation from seedings and anthers and organ differentiation from the callus of rice varieties and their hybrids. It appeared that in general the callus tissue was more easily induced in japonicas than in indicas with significant varietal differences. A highly significant positive correlation (r=0.896$^{**}$) was obtained between the fresh weight of callus induced in NAA medium and in 2, 4-D medium in the same variety. Callus formed on the medium with 2, 4-D was more friable than that formed on the medium with NAA. The callus formation from seedlings was better than from anthers in upland rice varieties. The easiness of the callus formation appeared to be a dominant character in crosses of rice varieties. Varietal difference was also noticed in the organ differentiation from the callus and the root differentiation seemed to be easier than the shoot differentiation. Most of the plants derived from the cal1us were albinos and the frequency of occurrence of albinos was greater in callus of seedlings than in that of anthers. Greater amount of callus tissue was formed from anthers of $F_1$ plants than anyone of their parents in remote-crosses but it was clear in close-crosses. A highly significant positive correlation (r=0.504$^{**}$) was found between the callus formation from seedlings and from anthers.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.23 no.2
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• pp.40-45
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• 1978

Degree of dominance for culm length, panicle numbers, flag leaf fwidth and panicle weight showed higher values than the others, and additive protions in variance components were more greater than heterosis protions for many characters. Average gene frequency showed low values for all characters, GCA were more greater than SCA, and the effects of GCA and SCA were differ from parents, crosses, generation and characters.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.25 no.1
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• pp.39-46
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• 1980

A male sterile facilitated recurrent selection population was developed by the barley breeding team of Yeongnam Crop Experiment Station. To breed this population, three composite cross populations and a composite population of breeding lines in our country were used as the materials. This population was developed in order to breed early maturing, wet-soil tolerant and high yielding barley varieties, adapted for double cropping with rice in southern part of Korea. The population was named by RSPYB#l which means Recurrent Selection Population No.1. of Yeongnam Barley.