• Title/Summary/Keyword: 교배율

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Changes of Plasma Progesterone and Estradiol-17${\beta}$ Level During Early Pregnancy in Immature Female Rats Superovulated by Transplantation of a Pituitary Gland (뇌하수체 이식에 의해 과배란된 미성숙 흰쥐에서 임신초기에 혈중 progesterone 과 estradiol-17${\beta}$ 수준의 변화)

  • Yang, Mhan-Pyo;Kim, Young-Hoon;Lee, Jang-Nag;Kwun, Jong-Kuk
    • Clinical and Experimental Reproductive Medicine
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    • v.13 no.1
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    • pp.53-58
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    • 1986
  • 본 연구는 한개의 뇌하수체를 이식시켜 과배란된 미성숙 흰쥐에서 혈중 progesterone과 estradiol-17${\beta}$ 의 수준 변화를 관찰하기 위하여 시도되었다. 30일령 숫컷 휜쥐에서 뇌하수체를 제거하기 15일 전에 고환을 제거시켰으며 고환이 제거된 쥐에서 얻은 한개의 뇌하수체를 실험 시작일(임신 3일전 : D-2) 오전 7시에서 10시사이에 28일령의 암컷 흰쥐의 우측 신장 피막 아래 이식시켰다. 대조군은 같은날 오전 10시에 4 IU PMSG 를 투여하였다. 실험에 사용된 쥐들은 혈중 호르몬 수준을 측정하기 위하여 임신 3일전, 2일전, 1일전, 임신 1일, 2일, 3일 및 5일에 희생시켜 채혈하였다. 임신 1일에는 교배후 estrogen의 과량분비를 차단하기 위하여 난소를 제거한 후 난소 호르몬을 투여하고 임신 8일에는 착상 상태를 조사하였다. 혈중 progesteron과 estradiol-17${\beta}$ 수준은 gamma counter(Packard)로 계측하였다. 본 실험에서 얻은 결과는 다음과 같다. 1. 난소를 제거하고 progesterone과 estradiol-17${\beta}$를 투여한 과배란된 흰쥐는 난소를 제거하지 않고 과배란된 흰쥐나 대조군에 비하여 효과적인 착상율을 보이지 않았다(P<0.001). 2. 과배란된 흰쥐에서 혈중 progesterone 수준은 대조군에 비해 교배후 계속적으로 높은 상승을 보였으나 교배전 수준은 대조군에 비해 낮았다(P<0.001). 3. 과배란된 흰쥐에서 혈중 estradiol-17${\beta}$ 수준은 과배란 2일전부터 임신 1일까지 아주 높은 상태를 유지하였으며 임신 1일전(발정전기)에는 638${\pm}$134 pg/ml 으로 절정을 나타내었으나 임신 1일 이후 부터는 급격히 감소하여 임신 5일에는 10pg/ml이하로 떨어졌다.

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Morphology and Characteristics of Floral Organ in Highbush Blueberry (Vaccinium corymbosum) Cultivars (하이부쉬 블루베리의 품종별 화기 특성 및 형태적 관찰)

  • Kim, Su Jin;Bae, Kang Soon;Koh, Sang-Wook;Kim, Ho Cheol;Kim, Tae-Choon
    • Korean Journal of Plant Resources
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    • v.28 no.2
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    • pp.235-242
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    • 2015
  • Morphology and characteristics of floral organ in highbush blueberry cultivars were studied to select suitable cultivars of highbush blueberry for domestic cultivation. The stamen consists an anther and a tape-like hairy filament with well-developed trichomes. When the anther was opened, the wall of anther was not dehiscent, and pollen grains were discharged into two tubes. Pollen was mature tetrad type without being separated after meiosis (Late March). The number of pollen granules per anther was 400~1,300, the germination rate was higher in the cultivars having many pollen grains. Pistil was composed of five carpels and a shipper without separate part. The number of ovules per ovary was 39~67, therefore, the coefficient of ranged from 11.6 to 31.0%. The seed pod formation by combination of ‘Bluejay’ and ‘Sharpblue’ was higher in the cross-pollination than in the self-pollination.

Effect of Crossbreeding of Korean Native Duck and Broiler Ducks on Performance and Carcass Yield (토종오리와 육용오리의 교배 조합이 생산성 및 도체 수율에 미치는 영향)

  • Kim, Hak-Kyu;Hong, Eui-Chul;Kang, Bo-Seok;Park, Mi-Na;Seo, Bo-Young;Choo, Hyo-Jun;Na, Seung-Hwan;Bang, Han-Tae;Seo, Ok-Suk;HwangBo, Jong
    • Korean Journal of Poultry Science
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    • v.37 no.3
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    • pp.229-235
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    • 2010
  • This work was carried out to improve the performance and the carcass yield of Korea native duck (KND) according to crossbreeding KND and broiler duck (BD). Two hundred forty out of crossbred ducks were divided into 4 strains and selected as 120 ducks separated by sex, respectively. Crossbred ducks were native ducks (female)${\times}$native ducks (male), native ducks (female)${\times}$broiler ducks (male), broiler ducks (female)${\times}$native ducks (male), and broiler ducks (female)${\times}$broiler ducks(male) as A, B, C and D, respectively. Broiler duck diet was fed as experimental diet for 0~3 weeks (CP 22.0%, ME 2,900 kcal/kg) and 3~8 weeks (CP 18.0%, ME 3,000 kcal/kg). Body weight of male was high compared to female, and that of B and C was high compared to A and low compared to D at 3 and 8 week (P<0.05). Body weight gain of male was high compared to female at 3~8 weeks and 0~8 weeks, and it was shown as D>C>B>A on body weight gain (P<0.05). In case of feed intake, there was no difference between male and female, and B and C were high compared to A and low compared to D (P<0.05). Male feed requirement was high at 3~8 weeks, and there was significant difference between A and B, C and between B and D. Carcass yield of 4 strains was shown as 70.1%, 70.8%, 73.1% and 71.2%, respectively. Finally, Crossbreeding of KND and BD may improve the performance and the carcass yield of KND.

Estimating the Parameters of Pollen Flow and Mating System in Pinus densiflora Population in Buan, South Korea, Using Microsatellite Markers (Microsatellite 표지를 이용한 부안지역 소나무 집단의 화분 유동과 교배양식 추정)

  • Kim, Young Mi;Hong, Kyung Nak;Park, Yu Jin;Hong, Yong Pyo;Park, Jae In
    • Korean Journal of Plant Resources
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    • v.28 no.1
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    • pp.101-110
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    • 2015
  • Parameters of mating system and pollen flow of a Pinus densiflora population in Buan, South Korea, were estimated using seven nuclear microsatellite markers. The expected heterozygosity ($H_e$) was 0.614 in mother trees and 0.624 in seeds. Fixation index (F) was 0.018 and 0.087 in each generation. There was no significant genetic difference between the generations (P > 0.05). From MLTR, the outcrossing rate ($t_m$), the biparental inbreeding ($t_m-t_s$), and the correlation of paternity ($r_p$) were 0.967, 0.057, and 0.012, respectively. tm was larger but $t_m-t_s$ and $r_p$ were smaller than those of allozyme markers in Pinus densiflora. These values were similar to those of microsatellite markers in other pine species. The optimal pollen dispersal model from TwoGener was the normal dispersal model with the effective density of 220 trees/ha and its level of genetic differentiation in pollen pool structure (${\Phi}_{ft}$) was 0.021. The average radial distance of pollen flow (${\delta}$) was calculated as 11.42 m, but no correlation between the pairwise-${\Phi}_{ft}$ and the geographical distance among mother trees was at Mantel test (r = -0.141, P > 0.05). Although the effective pollen dispersal in the population seems to be restricted, the amount of genetic variation might be maintained in each generation without a loss of genetic diversity. It might be because the genetic diversity in pollen pool was high but the genetic difference between pollen donors was small under the complete random mating condition in the Pinus densiflora population in Buan.

Mapping of UV-B sensitive gene in Arabidopsis by CAPS markers (CAPS marker에 의한 Arabidopsis의 자외선 B 감수성 유전자 지도작성)

  • 박홍덕;김종봉
    • Journal of Life Science
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    • v.12 no.6
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    • pp.715-720
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    • 2002
  • A mutant Arabidopsis thaliana which is very sensitive to Ultraviolet-B(UV-B) radiation has been isolated by ethylmethane sulfonate(EMS) mutagenesis. Genetic cross proved the UV-sensitive gene(uvs) to segregate as a single Mendelian locus. For mapping of uvs, we crossed Arabidopsis thaliana Lansberg with uvs plant(Columbia), and made F2 plants by F1 selfcross. We designed 10 kinds of CAPS marker primers. Each primers amplifies a single mapped DNA sequence from uvs and Lansberg erecta ecotyres. Also identified was at least one restriction endonuclase for each of these PCR product that generates ecotype-specific digestion pattern. We got crossing over value of UB-sensitivity and each CAPS marker which located on different chromosome arm. The value of crossing over showed that uvs was linked to LFY3 which was on chromosome 5.

Comparison of Agronomic Traits According to Plant Types before Wintering in Malting Barley Lines (맥주맥의 월동전 초형에 따른 주요 형질의 비교)

  • 천종은
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.36 no.1
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    • pp.12-16
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    • 1991
  • So as to compare the differences of major agronomic traits according to fall plant types, two cultivars and eight lines which were derived from the same pedigree, were planted and analyzed. The heading and mature times, and culum length of erect plant types were earlier or longer, but the spike number per ㎡ and yield were smaller than the those of prostrate types. Also, 1000 grain weith, assortment rate and light penetration rate in erect types were greater than in prostrate types. The heading time was positively correlated with mature time and yield, but negatively correlated with culum length, 1000 grain weight, assortment rate and light penetration rate. Yield was negatively correlated with light penetration rate. If the lines with prostrate types in fall season, fast regrowth after wintering and rapid elongation of stem were bred and selected, it will be of great benefit in the area requiring sever winter hardiness.

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Factors Affecting Seed Yield in Larix (낙엽송(落葉松)의 종자결실(種子結實)에 영향(影響)을 미치는 요인(要因))

  • Shin, Dongill;Karnosky, David F.
    • Journal of Korean Society of Forest Science
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    • v.84 no.2
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    • pp.207-217
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    • 1995
  • Various factors reducing seed yield in 4 Larix species throughout the whole reproductive cycle were investigated and partitionate losses attributed to them were determined. Pollen quality, lack of pollination, and degeneration of female gametophyte played minor roles in reducing seed yield. Failure of pollinated ovules to be fertilized was an important factor causing seed loss. Embryo degeneration was also a major factor causing seed loss in all 4 species. Strobili abortion, which causes loss of all potential seeds in a cone, was the most important factor in reducing seed loss in this study. Based on the results obtained from this studs, hybridizations in either direction between European larch and Japanese larch are likely to resulting viable seed. However, hybridization between tamarack as a mother tree and European larch are not likely to result in viable seeds being produced.

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Fuzzy Controller Design of 2 D.O.F of Wheeled Mobile Robot using Niche Meta Genetic Algorithm (Niche Meta 유전 알고리즘을 이용한 2자유도 이동 로봇의 퍼지 제어기 설계)

  • Kim Sung-Hoe;Kim Ki-Yeoul
    • The Journal of Information Technology
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    • v.5 no.4
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    • pp.73-79
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    • 2002
  • In this paper, I will propose the Niche-Meta Genetic Algorithm that has a multi-mutation operator for design of fuzzy controller. The gene in the proposed algorithm is formed by several parameters that represent the crossover rate, mutation rate and input-output membership functions. The optimization of fuzzy membership function is performed with local search on sub-population and the optimal structure is constructed with global search on total-population. The multi-mutation is selected under basis of the result of local evolution. A simulation for 2 D.O.F wheeled-mobile robot is showed to prove the efficiency of the proposed algorithm

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Oospore Production in Broth Media and Oospore Germination of Phytophthora capsici (고추 역병균(Phytophthora capsici)의 액체배지에서 난포자 형성과 발아)

  • Kim, Byung-Sup;Rin, Ernest James;Coffey, Michael D.
    • The Korean Journal of Mycology
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    • v.37 no.1
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    • pp.114-116
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    • 2009
  • In this study, we selected suitable broth media for mass production of Phytophthora capsici oospore, investigated oospore germination and secured $F_1$ progeny. Carrot broth and V8C broth were determined most effective for oospore formation by calculating and comparing oospore concentration produced from 8 different liquid media. Eleven strains were selected from P. capsici (CapA)/P. tropicalis (CapB) and 9 crosses were formed. Oospore progeny were produced, isolated and germinated from A1 and A2 combinations of P. capsici (CapA) with P tropicalis (CapB). This resulted in a total number of 129 $F_1$ isolates of P. capsici/P. tropicalis with a 0.64-4.0% (mean 1.85%) oospore germination.

Distributed Hybrid Genetic Algorithms for Structural Optimization (분산 복합유전알고리즘을 이용한 구조최적화)

  • 우병헌;박효선
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.16 no.4
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    • pp.407-417
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    • 2003
  • Enen though several GA-based optimization algorithms have been successfully applied to complex optimization problems in various engineering fields, GA-based optimization methods are computationally too expensive for practical use in the field of structural optimization, particularly for large- scale problems. Furthermore, a successful implementation of GA-based optimization algorithm requires a cumbersome and trial-and-error routine related to setting of parameters dependent on a optimization problem. Therefore, to overcome these disadvantages, a high-performance GA is developed in the form of distributed hybrid genetic algorithm for structural optimization on a cluster of personal computers. The distributed hybrid genetic algorithm proposed in this paper consist of a simple GA running on a master computer and multiple μ-GAs running on slave computers. The algorithm is implemented on a PC cluster and applied to the minimum weight design of steel structures. The results show that the computational time required for structural optimization process can be drastically reduced and the dependency on the parameters can be avoided.