• Korean journal of applied entomology
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• v.23 no.4 s.61
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• pp.203-208
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• 1984

Four varieties of tobacco, Nicotiana tabacum var. NC 2326. NC 95, NC 744 and Havana. and two plant species, Physalis floridana Rydb., N. repanda Wild. were used for classification of ten isolates of potato virus Y(PVY) obtained Iron potato or tabacco plants showing various symptoms. Each of the 10 isolates could be distinguished by the host reactions showing necrosis, vein banding and/or mottling, or no symptom on these hosts. Five isolates of PVY, PVY-VN, PVY-N, PVY-NSNR, PVY-Chile, and PVY-Argentina, showed necrotic symptom on NC 2326, but others showed vein handing and/or mottling symptom. On NC95, each of the isolates tested showed similar symptons as on NC2326, except necrotic symptom by the isolate PVY-MSNR. Havana showed mottling reaction to the PVY-NSNR, PVY-MSNR, PVY-MSMR, PVY-VB, and necrosis to PVY-Chile, PVY-Argentina, but showed no symptom to the others. NC744 showed mottling symptom to the PVY-MSNR and PVY-MSMR, necrosis to the PVY-Chile and PVY-Argentina, and no symptom to the others. On N. repanda, necrotic reaction to PVY-Argentina. no symptom to PVY-VN and PVY-C, and nettling to others were observed.

• Korean Journal Plant Pathology
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• v.3 no.3
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• pp.223-235
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• 1987

Samples showing yellow mosaic symptom of Lilium spp. with necrotic fleck, stunting, malformation, and colour breaking were collected from lily-growing areas in the southern part of Korea. Two viruses were distinguished under a electron microscope and their host range, serological reaction, stability in sap, type of aphid transmission, and relations with cells and tissues were examined. Broad bean wilt virus (BBWV) was transmitted by sap-inoculation to 23 plant species in 8 families and by the aphid, Myzus persicae. This virus was inactivated after 10 min at 70C, at dilution of $10^{-3}$, and after 6 days at about 20C. Electron microscopic examination of purified preparation showed that the virus is spherical particle of 28nm in diameter. The virus reacted positively with BBWV-antiserum in agar gel diffusion test. In ultrathin sections of BBWV infected tissues, large aggregates or crystalline array of virus particles and vesicular body were found in the cytoplasm, vacuole, and nucleus of mesophyll cells. Cucumber mosaic virus (CMV) was transmitted by sap-inoculation. Electron microscopic examination of its purified preparation showed spherical particles of 30nm in diameter. The virus reacted positively with CMV-Y strain-antiserum in agar gel diffusion test. In ultrathin sections of CMV infected tissues, crystalline array of virus particles were found in the vacuole and a large number 0f virus particles were found in the cytoplasm and the plasmodesmata of mesophyll cells. When each of these viruses was retransmitted to Lilium tigrinum. L. concolor, and L. auratum, BBWV induced slight symtoms and colour breaking, but CMV induced yellowing mosaic or necrotic fleck.

• Research in Plant Disease
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• v.19 no.1
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• pp.36-44
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• 2013

Carnation is considered to be one of the top three cutting flowers in the world, which is a main crop with 21 billion annual volume of manufacture. The four carnation items such as cuttings, seed, plant and unrooted cuttings are imported and exported. Viruses can be easily transmitted during vegetative propagation of carnation. Carnation necrotic fleck virus (CNFV) and Carnation ringspot virus (CRSV) are designated as Korea plant quarantine viruses and inspected. This study was aimed to develop specific primer sets for easy and rapid detection of CNFV and CRSV. Two RT-PCR primer sets were efficiently amplified 288 and 447 bp fragments for CNFV and 503 549 bp fragments for CRSV. Furthermore, developed nested primer sets make possible to high sensitive detection and verification. CNFV nested PCR primer sets all produced band of 147 bp and CRSV nested PCR primer sets did bands of 395 and 347 bp. In addition, plasmid inserted 6 sequences in amplicon were used as a positive control to improve inspection confidence. The successful application of PCR module newly developed in this study will be highly useful for detect of CNFV and CRSV for quarantine inspections.

• Research in Plant Disease
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• v.8 no.2
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• pp.101-106
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• 2002

For the detection of Tobacco masaic virus (TMV), Tomato mosaic virus (ToMV), and Cucumber mosaic virus (CMV), tomato seeds of 11 table tomato and 7 cherry tomato cultivars were assayed by DAS-ELISA. Among the cultivars, TMV and ToMV were detected from 9 cultivars at the rates lower than 20% and 16%, respectively. In the assay on seed transmission rates, ToMV and CMV were detected as high as 24% and 8% , respectively, but TMV was not detected. In field survey on these viruses from tomato plants of 10 different places in Chungbuk province, ToMV and CMV were detected from most fields. TMV was detected from only 3 fields. The highest detection rates of these viruses were recorded in Cheongwon for TMV Chungju for ToMV, and the other locality of Chungju far CMV. It was difficult to find any relationship between the growth stage of tomato and infection rates. TMV usually caused mosaic on leaves while ToMV caused various symptoms including yellows, necrosis, and mottling. CMV-infected tomato plants showed symptoms of shoestring, fern leaf, and yellows.

• Korean Journal of Agricultural Science
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• v.3 no.1
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• pp.85-94
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• 1976

Six cases of gangreous mastitis of goats infected spontaneously were observed clinically and pathologically in Daegu and Daejeon district. and with strain isolated purely from the infected goats, the artificial infection to the animal was examined, the sensitivity of strain to the antibiotics was tested and clinical treatment was carried out. The results obtained are summarized as follows: 1. In the six cases approximately same clinical findings were observed as the previously published literatures on gangrenous mastitis of cattle, sheep and goats. 2. The micrococcus pyogenes var aureus was highly virulent strain which was the causative organism for the gangrenous mastitis by inoculating in the udder. 3. The gangrenous mastis was probably occured by the formation of thrombosis in veins of udder. 4. In the sensitivity test, the micrococcus pyogenes var aureus resited for penicillin in 2 cases among the 6 strains, but sensitived for streptomycin, chloromycin, oxyteracycline, erythromycin, achromycin, neomycin and kanamycin in other 4 and in all case. 5. The treatment for gangrenous mastitis may be extirpated the gangrenous region surgically in the case of unilaterally or locally affected, treated by muscle injection or teat-operation in the case of severely or diffusely affected and infused antibiotics up to teat canal or treated by mammary tissue injection in the case of slightly affected.

• Current Research on Agriculture and Life Sciences
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• v.9
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• pp.115-125
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• 1991

The medicinal plants of 33 species with symptoms of mosaic, necrosis, line pattern, malformation (fine leaf), stripe, and ring spot infected by cucumber mosaic virus spontaneously, were collected in Korea and Japan. Among them, 2 mosaic diseases of portulaca oleracea L. and Celocia argentea L., 2 necrotic mosaic diseases of Aristolochia debilis Sieb. and Tetragonia exponsa Murr., 6 mottle diseases of Cassia torosa Cav., Bupleurum falcantum L., Angelica acutioloba Kitagawa, A. keiskei Koidz., Peucedanum japonicum Thunb., and Foeniculum vulgare Mill., and ringspot disease of Basella rubra L., were named newly in these studies.

• Korean Journal of Microbiology
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• v.31 no.1
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• pp.79-84
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• 1993

Infection of Chinook Salmon Embryo (CHSE) cells with IPNV resulted in a significant decrease in intracellular free calcium concentration ([$Ca^{2+}$]i) compared to mock-infected cells. The degree of the decrease in [Ca$^{2+}$]i was dependent on the amount of input virus, and treatment of IPNV-infected CHSE cells with metabolic inhibitors such as cyloheximide cordycepin partially reversed the decrease in [$Ca^{2+}$]i in IPNV-infected cells. Inactiation of PINV with UV also abolished IPNV-induced decrease in [$Ca^{2+}$]i. These data suggest an active role of IPNV in the decrease of [Ca$^{2+}$]i in the infected CHSE cells. The importance of the decrease in [$Ca^{2}$i] could be supported by the finding that the production of IPNV plaques increased in the cells treated with verapamil, a calcium influex blocker, and by lowering the concentration of extracellular calcium. Decreased production of IPNV plaques was observed by elevating the extracellular calcium. Thus, it is suggested that IPNV induced a decreased in [$Ca^{2+}$]i and the decrease in [$Ca^{2+}$]i may plan an importat role in efficient replication of IPNV.ation of IPNV.

• Research in Plant Disease
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• v.12 no.2
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• pp.148-151
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• 2006

Tomato spotted wilt virus (TSWV) was occurred newly on the 4 ornamental plants of Impatiens balsamina (Balsam), Dahlia variabilis (Dahlia), Callistephus chinensis (Aster) and Zinnia elegans (Indian lilac) at Anyang area in Korea. TSWV produced the typical symptoms of single or double ring spots on the leaves and induced usually necrosis, wilt and/or severe mosaic.

• Tuberculosis and Respiratory Diseases
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• v.57 no.4
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• pp.381-385
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• 2004

Pulmonary gangrene is a rare and severe complication of bacterial pneumonia, where a pulmonary segment or lobe is sloughed due to parenchymal devitalization of the parenchyma, with secondary anaerobic infection and necrosis caused by pulmonary vascular thrombosis. Prior to the antibiotic era, massive pulmonary gangrene was potentially fatal. Herein, a case of pulmonary gangrene in a 67-year-old man is reported. He complained of fever, chills, dyspnea and purulent sputum of 5 days duration. The plain chest radiograph showed well-marginated right upper lobe consolidation, with bulging minor fissure, suggestive of a Klebsiella infection. A contrast CT scan demonstrated consolidation of the right upper lobe, with a central necrotizing portion. Klebsiella species was confirmed from both sputum and blood cultures. After appropriate antibiotics, the chest X-ray and CT scan 3 weeks later showed a large cavity with an air-fluid level, sloughing-off and extrusion of necrotic lung tissue, suggestive of pulmonary gangrene. Seven months later, the right gangrenous lung showed severe volume loss on a chest radiograph. The management of pulmonary gangrene has been somewhat controversial. Herein, it was managed without surgical drainage or resection. If the antibiotic therapy had failed, then a surgical approach would have been considered.

• Korean journal of applied entomology
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• v.23 no.4 s.61
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• pp.209-214
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• 1984

Since 1980, burley tobacco plants grown mainly in the western hat of the Korea. have shown two new types of disease symptom. Both symptoms were found to be caused by two different PVY strains : the vein banding type by a PVY strain designated as PVY-VB and necrosis on leaf veins by a PVY strain designated as PVY-VN, Identification of the PVY strains was based on host range test. aphid( Myzus Persicae) transmission test, physical properties, serology, and observation of virus particle morphology. The virus particles were measured to be about 730 nm without any difference in shape or dimensions between the two strains. Both strains also gave a positive reaction to the PVY antiserum in SDS-agar gel double diffusion test. These strains, however, gave a negative reaction to the tobacco etch virus and tobacco vein mottling virus antisera.