• The Journal of the Korean Society for Microbiology
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• v.9 no.1
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• pp.33-40
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• 1974

On the basis of pigment production and growth rate on L-J medium containing crocin, differentiation of opportunist mycobacteria belonging to photochromogens, scotochromogens, nonchromogens and rapid grower has been investigated. Among photochromgens, positive pigmentation of M. kansasii was differentiated from negative strain of M. marinum, Scotochromogen M. aquae was positive whereas M. scrofulaceum was negative. Rapid grower M. fortuitum was positive at 3 days test whereas M. smegmatis was negative. Subdivision of opportunist mycobacteria into four groups on the basis of growth rate and pigment production on L J medium containing gardenia extraction appeared to be a valuable adjunct to the Runyon's classification for the rapid presumptive identification of opportunist mycobacteria of different clinical significance.

• Korean Journal of Optics and Photonics
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• v.16 no.2
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• pp.146-151
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• 2005

We fabricated pyrromethene 597(PM597) solid-state dyes which were dissolved in PMMA(poly- methylmethacrylate) and characterized the lasing characteristics. The laser was end-pumped by a frequency doubled Q-switched Nd : YAG laser. We obtained the slope efficiency of $76\%$ at lasing wavelength of 585 nm. We demonstrated the laser lifetime to be more than 100,000 shots with output energy of 250 mJ and repetition rate of 4 Hz by adding singlet oxygen quencher DABCO(1,4-diazabicyclo [2,2,2]octane) to the solid-state dye.

• Korean journal of applied entomology
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• v.34 no.4
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• pp.334-343
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• 1995

The eyes of Nilaparvata lugens were examined for ultrastructural changes in the light and dark adapted states. Inspection of light microscope sections taken at similar levels of compound eyes from insects kept in light or darkness for periods up to 72 hors revealed some differences between light and dark adapted eyes. Using the electronmicroscope, in light adapted eyes the palisade layer was narrower than that in dark adapted eyes. The pigment granules still formed a ring around the palisade layer in the dark adapted eye but, they did not form a tight circle around the rhabdom. No constant difference was found between the diameters of the microvilli in light and dark adapted eyes. The pigment movements at the junction of the cone and the rhabdom took the effect on varying the pigment aperture at the tip of the cone in front of the rhabdom tip.

• Korean Journal of Environmental Agriculture
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• v.11 no.3
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• pp.261-268
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• 1992

The membranes of mitochondria and chloroplasts contain a number of pigments that can act as endogenous sensitizers to produce activated oxygen species, most efficiently in blue light, which, in turn, attack functional targets in membranes. Therefore, intense blue light from the sun can exert various adverse effects on the functional and structural integrity of the membranes: one of the biochemical events of these negative effects could be the oxidative degradation of the unsaturated fatty acid constituents of membrane polar lipid. It may be assumed that as a strategy to avoid the light induced fatty acid degradation in membranes plant cells, responding to high intensity blue light, change the fatty acid compositions of membrane lipid in such that more-unsaturated fatty acid constituents are replaced by lessunsaturated fatty acid constituents. The results obtained in the present study, most importantly the measurements of double bond index of membrane polar lipid in concert with other measurements such as light quaility-dependent membrane peroxidation and the activities of membrane-bound proteins, seem to support this assumption.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.29 no.2 s.43
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• pp.1-35
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• 2003

새 천년으로 들어선 2000년도부터 우리나라의 65세 이상 노령인구는 $7.1{\%}$를 차지하게 되어 본격적인 노령화사회 (UN에서 65세 이상인구가 총 인구의 $7{\%}$ 이상일 경우 노령화 사회로 정의)로 진입하였다. 평균수명의 계속적인 증가에 따라 노인 인구는 급격히 늘어날 전망이며, 따라서 노인성 질환의 발생도 늘어나게 되어 가정 또는 사회가 떠 맡아야할 경제적, 정신적 부담은 더욱 증가하게 된다. 건강하고 행복한 인생의 황혼기를 보내는 것은 인간의 권리이며 누구나 바라는 소망이다. 피부의 노화현상은 심장질환, 암 등에 못지 않게 중요하며, 노인의 경제적, 사회적 활동을 심각하게 위축시키고 있다. 피부노화를 예방하고 노인성 피부질환을 치료할 수 있는 방법을 개발함으로써 노인의 경제, 사회활동을 활동적으로 유지시킬 수 있다. 피부노화는 크게 두 종류로 나눌 수 있다. 그 한가지는 내인성노화(intrinsic aging)로서 세월이 흘러감에 따라 피할 수 없는 노화 현상을 말한다. 두번째는 광노화 (photoaging)로서 오랫동안 햇빛에 노출된 얼굴, 손등, 목뒤 등의 피부에서 관찰되는 노화현상을 말하는 것으로 내인성노화 현상과 자외선에 의한 영향이 합쳐진 결과로 발생한다. 광노화 현상은 자외선의 노출을 피하면 예방할 수 있는 피부노화 현상이다. 내인성 노화는 햇빛에 노출되지 않은 피부에서 주로 관찰된다. 임상적 특징은 비교적 경미하며, 잔주름, 피부건조증, 탄력감소 등을 들 수 있다. 그러나 광노화의 임상적 특징은 내인성 노화에 비하여 심하고, 일찍부터 관찰된다. 내인성 노화에 비하여 굵고 깊은 주름이 발생하며, 잔주름도 많이 발생한다. 햇빛에 노출된 피부에 불규칙한 색소침착이 발생하며 일광흑자 (solar lentigo) 등의 색소질환이 증가한다. 피부가 매우 거칠고, 건조해지며, 탄력성이 감소하여 심한경우 피부가 처지게 된다. 피부노화의 대표적인 증상은 주름살이며, 아직까지 그 발생기전에 대하여는 여러 가지 학설이 있으나 정확히 알려져 있지 않다. 피부에 존재하는 교원질, 탄력섬유등 기질단백질의 손상이 피부 주름살의 주 원인으로 알려져 있다. 또한 얼굴에 존재하는 근육의 분포와 움직임, 유전적 소인, 자외선, 흡연, 폐경, 산화적 손상, 열 등 여러 원인이 복합적으로 작용할 것으로 생각된다. 피부주름살의 원인을 밝히고, 원인인자가 피부주름살을 초래하는 분자생물학적 기전을 이해함으로써 주름살을 예방하고 치료할 수 있는 새로운 방법을 개발할 수 있다.

• Journal of Applied Biological Chemistry
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• v.64 no.3
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• pp.197-203
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• 2021

Saponarin is a crucial component of barley sprout, and the production and quantitative analysis are issued to date. In this study, the optimal saponarin extraction conditions were presented on the subject of acetonitrile, ethanol, methanol, and water for the quantitative analysis in barley sprout through the extraction efficiency compared with the solvent concentration and extraction time using the reaction surface methodology. The optimal extraction time and solvent condition for saponarin were 3.9 h and 53.7% of aqueous methanol, respectively. In addition, the effect of LED artificial light on the saponarin production in barley sprouts was evaluated by the light cycle, light quantity, and light quality. The optimal cultivation conditions under artificial light for the growth of barley sprout and saponarin production were most effectively achieved on 220-320 μmol m^-2 s^-1 of the light quantity with 8 h day^-1 of a daylight cycle under 6500K LED combined with red light. Furthermore, blue light was evaluated as the main factor in the biosynthesis of saponarin.

• Korean Journal of Optics and Photonics
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• v.5 no.2
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• pp.266-271
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• 1994

The output power and the energy of Rh-6G dye laser were enhanced by the mixture of Rh-560 dye whose fluorescence spectrum was coincident with the absorption spectrum of Rh-6G. The argon filled coaxial flashlamp used for pulsed pumping and argon laser for CW pumping. The concentration of Rh-6G dye was optimized in each pumping method before Rh-560 dye was mixed in Rh-6G dye solution. In the coaxial flash lamp pumped Rh-6G laser the output energy was increased about 30% when Rh-560 was mixed at 1% of Rh-6G concentration. In the case of argon laser pumping with multiline, the output power was increased 18% at the concentration of 2.5%. In the single line laser pumping, the output power was enhanced more efficiently. The power enhancements were 72% and 88% when the pumping wavelengths were 488 nm and 514.5 nm respectively. ively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.23 no.2
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• pp.113-117
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• 1978

An attempt was made to establish a mass-screening technique for resistance to purple seed stain .disease in soybean. Seeds sterilized in 1 : 10000 'mercuric chloride for 1 minute and transffered to Petridishes containing 20ml water agar plus 50ppm of the sodium salt of 2,4-D were inoculated by placing a small agar cube containing fungal mycelium on the seed coat of each seed. A positive correlation between natural infection of purple seed :stain and purple discoloration by seed inoculation technique was highly significant and by this technique, some native soybean collections and introduced varieties were tested for resistance to the disease. Most of the soybean varieties tested were susceptible except for the varieties Hill, Harosoy and Sac, resistant comparatively.

• Journal of Environmental Science International
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• v.4 no.4
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• pp.335-344
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• 1995

Developmental changes of chlorophyll-protein complexes (CPs) and plastid membrane proteins in greening mung bean cotyledons and the effect of spermine therein were examined by SDS-polyacrylamide gel electrophoresis. The changes in the amounts of CPs became larger with the progress of greening and light-harvesting chlorophyll a/b protein (LHCP) was the main CP in the early greening stage up to f h. As the greening proceeded, chlorophyll-protein of the photosystem I (CPI) accumulated. Application of spermine were effective in accumulating CPs of the thylakoid membrane in the early phase of greening. In the profiles of the plastid membrane proteins, quantitative and qualitative changes were observed with the onset of greening up to 72 h. 56 kD protein of major intensity was observed in all greened chloroplasts and 24 kD protein increased remarkablly in both control and spermine-treated cotyledons. The thylakoids from spermine-treated cotyledons showed hither amounts of thylakoid proteins as compared to the controls. The results suggest that spermine may play a role in the regulation of plastid development and stabilizes the membrane function during greening.

• Journal of Environmental Science International
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• v.4 no.4
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• pp.33-33
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• 1995

Developmental changes of chlorophyll-protein complexes (CPs) and plastid membrane proteins in greening mung bean cotyledons and the effect of spermine therein were examined by SDS-polyacrylamide gel electrophoresis. The changes in the amounts of CPs became larger with the progress of greening and light-harvesting chlorophyll a/b protein (LHCP) was the main CP in the early greening stage up to f h. As the greening proceeded, chlorophyll-protein of the photosystem I (CPI) accumulated. Application of spermine were effective in accumulating CPs of the thylakoid membrane in the early phase of greening. In the profiles of the plastid membrane proteins, quantitative and qualitative changes were observed with the onset of greening up to 72 h. 56 kD protein of major intensity was observed in all greened chloroplasts and 24 kD protein increased remarkablly in both control and spermine-treated cotyledons. The thylakoids from spermine-treated cotyledons showed hither amounts of thylakoid proteins as compared to the controls. The results suggest that spermine may play a role in the regulation of plastid development and stabilizes the membrane function during greening.