• Korean Journal of Food Science and Technology
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• v.35 no.4
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• pp.690-695
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• 2003

Physiological activities of 40% ethanol extracts of Phellinus ribis were studied by employing several biological and biochemical assays. The extracts of Phellinus ribis displayed nitrate-scavenging activities (NSA) at pH 1.2 as with 64% NSA with 1.0 mg/mL of the extracts. They also had 91% 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities at the concentration of 1.0 mg/mL. Antioxidant activities of the extracts (at 0.5 mg/mL) on the autoxidation of linoleic acid (p<0.001) were also observed.. The inhibitory effect of the extracts on angiotensin converting enzyme was 11%. Cytotoxic effects of Phellinus ribis extracts against human cancer cell tines were also examined using MTT assay. The extracts (at 50 mg/mL) had severe growth inhibitory effects on A549, Hela, AGS, and SK-Hep-1, which were 8, 44, 76 and 42%, respectively. Ames test indicated that the extracts had no mutating effects on Salmonella typhimurium TA98 and TA100.

• Proceedings of the KSCN Conference
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• 2003.11a
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• pp.1073-1074
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• 2003

본 연구는 고학력 중년 여성을 대상으로 취업 여부에 따른 영양섭취 상태의 차이를 분석하여 고학력 중년 여성의 영양 및 건강관리를 위한 대책 마련의 기초자료로 활용하고자 시행되었다. 연구 대상자의 선정은 유의추출 방법으로 전업 여성은 고학력자 비율이 높은 대전시의 일정 지역에 거주하는 기혼여성으로 하였고, 취업여성은 여러 직업의 특성으로 인한 교란(confounding)을 줄이기 위하여 고학력 여성의 비율이 높은 기혼의 교사로 한정하였다. 연령에 의한 교란을 방지하기 위하여 연령대별로 동일 비율로 무작위 추출하여 전업여성 250명, 취업여성 236명을 대상으로, 24시간 회상법을 이용하여 조사 전일 하루동안의 식이 섭취 내용을 개별 면담으로 조사하였다. 1일 영양소 섭취 조사 결과 취업여성은 전업여성에 비해 인, 비타민 B$_1$, B$_2$를, 전업여성은 취업여성에 비해 지방 및 동물성 지방, 나트륨, 비타민 C, 엽산을 많이 섭취하고 있었다. P/M/S비는 전업여성 1/1.19/1.05, 취업여성 l/l.05/0.87로 나타나 취업여성이 전업여성에 비해서, 그리고 한국인 영양권장량의 기준(1/1/1)에 비해서 포화지방의 비가 낮았다. 또한 지방 에너지 구성비도 전업여성(24.38%)이 취업여성(23.29%)에 비해 높았고, 총 열량에 대한 동물성 지방의 섭취비율이 전업여성 12.44%, 취업여성 11.46%으로 나타나 전업여성이 취업여성에 비해 동물성 지방, 포화지방비, 지방에너지 구성비가 모두 높았다. 영양권장량에 대한 영양소 섭취 백분율에서 전업여성과 취업여성 모두 칼슘과 아연을 권장량에 비해 낮게 섭취하고 있었고, 질적 영양지수(INQ)도 두 군 모두에서 칼슘이 가장 낮게 나타났다. 인의 영양밀도(Nutrient Density)가 취업주부에서 높았던 점 외에는 영양밀도와 영양소 적정섭취비(NAR) 모두에서 비타민 B$_1$, B$_2$가 전업여성에 비해 취업여성이, 비타민 C가 취업여성에 비해 전업여성이 더 높았다. 한편 두 군 모두 INQ가 가장 높은 영양소는 인이었고, INQ가 가장 낮은 영양소는 칼슘으로 나타났으며, INQ가 1 미만인 영양소는 칼슘과 함께 철분으로 나타났다. 이는 1998년도 국민건강영양조사 결과에서 45세 이상 여성의 경우 관절염, 요통.좌골통, 디스크 등 근골격계질환 유병률이 남자보다 3배정도 높게 나타난 결과에 비추어볼 때 이들 관련 영양소에 대한 섭취 방안 지도의 필요성을 제시하고 있다. 결론적으로 고학력 중년 여성중 전업여성에 대해서는 지방 특히 동물성 지방 식품 섭취에 대한 적절한 영양교육과 식사관리 지도가 요구된다 하겠다. 또한 고학력 중년 여성은 취업 여부에 관계없이 칼슘과 철분이 많이 함유된 식품으로 식사량을 증가시켜 주는 영양 지도 및 영양 보강 대책이 요구되며, 칼슘과 인의 적정섭취비가 되도록 인의 섭취를 낮추는 식생활 지도가 필요하다.

• Journal of Life Science
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• v.28 no.12
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• pp.1455-1460
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• 2018

Due to a rapidly expanding aging population, the incidence of degenerative bone disease has increased, and efforts to handle the issue using regenerative medicine have become more important. In order to control various bone diseases such as osteoarthritis and osteoporosis, regenerative medicine utilizing adult stem cells has been extensively studied. And it is now clear that the mitochondrial energy metabolism, oxidative phosphorylation, is important for the process of stem cell differentiation. Interestingly, a recent study reported that salicylate promotes mitochondrial biogenesis by regulating the expression of $PGC-1{\alpha}$ in murine cells. However, the possible effects of salicylate on osteogenic differentiation through increased mitochondrial biogenesis in stem cells remain unknown. Thus, here we investigated whether salicylate could influence osteogenic differentiation and mitochondrial biogenesis of periosteum-derived mesenchymal stem cells (POMSCs). We found that salicylate treatments of POMSCs undergoing osteogenic differentiation increased the activity of alkaline phosphatase, a well-known early marker of bone cell differentiation. In addition, we observed that mitochondrial mass was increased by salicylate treatments in POMSCs. Together, these results indicate that salicylate can enhance osteogenic differentiation and mitochondrial biogenesis in POMSCs. Therefore, the findings in this study suggest that small molecules augmenting mitochondrial function such as salicylate can be a novel modulator for osteogenic differentiation and regenerative medicine.

• Journal of Nutrition and Health
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• v.41 no.1
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• pp.65-76
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• 2008

This study was performed to identify association between nutritional status and health risks of the elderly. This was a cross-sectional study involving low income elderly women in Gwangju, Korea (${\geq}$65y, n = 92). Socio-demographics, life style characteristics, health conditions, dietary intakes based on 24h-recall method, anthropometric measures, and clinical biochemistry parameters were examined. Anthropometric and clinical parameters included wt, ht, waist, hip, body protein, body fat, abdominal fat, total cholesterol, HDL-cholesterol, triglyceride, total protein, albumin, hemoglobin, hematocrit, fasting blood glucose, ferritin, IL-2, IL-6, TNF-${\alpha}$, CRP, TAS, TBARS, systolic blood pressure, and diastolic blood pressure. The subjects were divided into three groups based on age (65-74y, 75-84y, 85y${\leq}$) and were divided into two groups according to the sum of the Nutrition Screening Initiative (NSI) checklist score (adequate nutritional status, NSI score ${\leq}$3; at risk of malnutrition, NSI score >3). Mean and frequency of variables were estimated. Analysis of Variance, Tukey test, Chi-square test, and Multiple linear regression analyses were performed. Mean BMI and body fat were 25.1 $kg/m^2$ and 40.0%, respectively. However, for over 80% of subjects, the intakes of energy, fiber, thiamin, riboflavin, niacin, folate, Ca, K, and Zn were less than the Korean DRI (EAR or AI). The subjects who had lower NSI score tended to have better health status, eat meals frequently, have less depression, and exercise regularly. The subjects who had higher NSI score tended to have tooth problems, to eat alone most of time, and to be physically unable to cook or feed. Serum IL-6 and TNF-${\alpha}$ were significantly related with nutritional status which suggested higher tendency of inflammatory response. Serum IL-2, TAS, and glucose were significantly correlated with body fat (%) or abdominal fat (%). These results suggest that improving the nutritional status, increasing regular exercise, maintaining normal weight are beneficial to health care of low income elderly women.

• Journal of agricultural medicine and community health
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• v.38 no.3
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• pp.182-194
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• 2013

Objectives: To identify and compare the health behaviors and health status of the elderly between urban and rural areas using the data of the Korean National Health and Nutrition Examination Survey (KNHANES). Methods: The study population comprised 3,823 elderly people aged 65 years or older who participated in the $4^{th}$ KNHANES (2007-2009). The areas were classified into "large cities," "cities," and "rural areas" using the administrative and residential areas. The health behaviors and health status of the elderly between the rural and urban areas were compared using a complex sample design with the Rao-Scott chi-square test and weighted multiple logistic regression analysis. Results: Compared to large cities, the odds ratios (ORs) (95% confidence interval [CI]) of rural areas were as high as 1.58 (1.25-2.01) for the influenza vaccination and as low as 0.47 (0.37-0.59) for flexibility exercises, 0.56 (0.38-0.81) for muscular exercises, and 0.76 (0.62-0.92) for obesity. The ORs (CI) for osteoarthritis and diabetes mellitus were as low as 0.81 (0.66-0.99) and 0.70 (0.55-0.89), respectively. Conclusions: The health behaviors and health status of the elderly are better in rural areas than in urban areas despite the fact that the socioeconomic conditions in rural areas are poorer that those in urban areas. These findings suggest that programs suitable for residential areas should be developed and that studies to explain the differences in residential areas are needed.

• Analytical Science and Technology
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• v.28 no.5
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• pp.323-330
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• 2015

Alkaptonuria, a rare inherited metabolic disease, is characterized by a lack of homogentisate dioxygenase and accumulation of homogentisic acid (HGA), leading to homogentisic aciduria, arthritis, and ochronosis. In this study, a rapid analytical method, without an expensive and tedious solid phase extraction step, was developed to quantify HGA in plasma using GC-MS. HGA-spiked pooled plasma samples were subjected to liquid-liquid extraction (LLE) with ethyl acetate, followed by trimethylsilyl derivatization (TMS) and GC-MS quantification using selected ion monitoring. The formation of TMS derivative of the 1 carboxylic and 2 hydroxyl functional groups was performed by reacting BSTFA (with 10% TMCS) for 5 min at 80 ℃. For selected ion monitoring, quantification and confirmation ions were determined based on specific ions (m/z 384, m/z 341 and m/z 252) of the TMS derivative of HGA. Calibration curves of pooled normal plasma specimens showed a linear relationship in the range of 1-100 ng/µL. The precision and accuracy were within a relative standard deviation (RSD) of 1 to 15% and a bias of -5 to 25%. Recoveries were obtained in the range of 99-125% and 95-115% for intra-day and inter-day assay, respectively, at 2, 20 and 80 ng/µL. The limit of detection (LOD) and limit of quantification (LOQ) were 0.4 ng/µL and 4 ng/µL, respectively. No homogentisic acid was excreted from normal Korean plasma samples. Collectively, the results from the present study suggest that this method could be useful for routine diagnosis and therapeutic monitoring of alkaptonuria patients with excellent sensitivity and rapidity.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.7 no.1
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• pp.54-60
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• 2004

Purpose: Henoch-$Sch{\ddot{o}}nlein$ purpura (HSP) is a small-vessel vasculitic disease that most often affects the skin. Abdominal symptoms precede the typical purpuric rash of HSP in 14~36%. It is a challenge to diagnose HSP in the absence of a rash, because there are no biologic tests that can identify HSP with certainty, so we tried to find out the characteristic features of HSP gastroenteropathy without purpura before diagnosis. Methods: This study included 82 children with HSP who had been admitted or visited outward of the Department of Pediatrics, Pusan National University Hospital from 1995 to 2000. The cases that the onset of purpura preceded or coincided that of abdominal pain were defined as purpura-positive group. The cases that the onset of abdominal pain preceded purpura more than 1 week and purpura was not presented till diagnosed as HSP gastroenteropathy were defined as purpura-negative group. We compared and analyzed the clinical features of the two groups by reviewing the medical records retrospectively. To ensure the diagnosis of HSP gastroenteropathy, we conducted upper GI series, abdominal ultrasonogram, abdominal CT, endoscopy and/or skin biopsy. Results: The number of cases of purpura-positive group and purpura-negative group were 72 and 10, respectively. There is no difference between two groups in the incidence of clinical symptoms and laboratory findings. Children with HSP gastroenteropathy had characteristic erosive or ulcerative lesions in the stomach or duodenum on esophagogastroduodenoscopy, or mural thickening of the small bowel on abdominal ultrasonogram, CT or upper GI series. Skin biopsy revealed leukocytoclastic vasculitis in 3 of them, although biopsy specimen was taken from any areas of normal- appearing skin. In purpura-negative group, 9 patients improved by steroid therapy. Conclusion: In purpura-negative group, there is no diagnostic feature on the laboratory findings and clinical features. Therefore, to diagnose HSP gastroenteropathy in patients with abdominal pain in the absence of the characteristic rash, careful observation of clinical features and laboratory data, and prompt application of available diagnostic tools such as gastrointestinal endoscopy, radiologic study and skin biopsy are recommended. Early use of corticosteroid may reduce the suffering in these patients.

• Journal of agricultural medicine and community health
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• v.25 no.2
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• pp.427-440
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• 2000

A comparative study was made about health resources, medical care service statistics and public health service statistics by health subcenters at Jangdong and Jangpyung townships, Jangheung County, Chollanamdo before and after the unification of two health subcenter to improve their function. 1. While two general physicians, one dentist, 4 nurse aids arid one oral hygienist were working at two health subcenters with simple facility with examination room and public health office in 1997 prior to the unification, in 1999 after the unification of two health subcenters 14 staff including a specialist physician, a general physician, a dentist, a herb hygienist, a radiology technician and a physical therapist were working in the new health subcenters equipped with appropriate facilities in two storey building. 2. In 1997 before the unification the yearly total income of two health subcenters was 78,815 thousand won(about 14,000 won per capita) and the amount was 140,376 thousand won(about 25,000 won per capita) in 1999 after the unification. And the income was used for operation of health subcenters excluding personnel expense. 3. While 90.5% of visitors to the health subcenters came for general medical care, and 91.6% came for the revisit before the unification, after the unification 71.2% came for general medical care, 10.8% for dental care, 16.5% for oriental physician's care, 29.7% for the first visit and 70.3% for revisit. Most common problem cared for was musculoskeletal disorder like arthralgia. Average treatment cost per person per month was 9,363 won before the unification and 8,309 won after the unification. 4. Through the comparison of execution rate of public health services before and after the unification. the practice rate of most health service among target population including visiting service for chronic illness, maternal and child health service and immunization service increased after the unification. The practice rate of tuberculosis control service, hypertension control and diabetes management was a little decreased. In conclusion, continuous effort to satisfy all persons in two townships and evaluation are necessary to coincide with the spirit of unification of two health subcenters.

• The Korean Journal of Food And Nutrition
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• v.27 no.5
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• pp.872-880
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• 2014

This study was conducted to assess the characteristics and satisfactions related with facility foodservice for the elderly in the Northern Gyeonggi-do area (Republic of Korea). To accomplish this, a survey was conducted to investigate the general characteristics of the elderly, health information, and satisfaction with the foodservice of a welfare facility in the area. The main sources of health information for both the male and female elderly included 'health professionals' (49.0, 45.7%) and the 'mass media' (34.4, 33.5%), and they were revealed to trust the information from 'health professionals' (65.6, 59.5%), more so than the 'mass media' (19.8, 20.8%). The principal diseases of the elderly were 'hypertension' (27.1%), 'diabetes' (21.9%), 'palsy' (19.8%) in males, and 'hypertension' (32.4%), 'diabetes' (24.9%), 'arthritis' (15.0%) in females. Most male and female elderly indicated the main reasons for skipping a meal to be 'no appetite' (37.5, 53.8%) and 'indigestion'(19.1, 17.3%), respectively. The facility foodservice was used by the male elderly for the reasons of 'irritating to cook' (28.1%), 'to meet a friend' (26.0%), and 'economic' (14.6%), while the female elderly used it for the reasons of 'economic' (25.4%), 'to meet a friend' (23.1%), and 'irritating to cook' (23.1%). The reasons for satisfaction with the facility were subject to 'meal supply' (28.0, 37.2%), 'social exchanges' (20.0, 22.3%), and 'good facilities' (18.7, 18.2%). Regarding the officers and nurses of the facility, 13.3% and 9.3% of males and 8.3% and 12.4% of females indicated satisfaction, respectively. The satisfaction with foodservice influenced the welfare facilities, and providing a successful environment for foodservice requires strengthening of the education of facilities employees to become friendlier. Based on these results, greater efforts should be made to provide meaningful information regarding the facility foodservice for the elderly related to the silver service industry in the Northern Gyeonggi-do area as soon as possible.

• Journal of the Korean Arthroscopy Society
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• v.11 no.1
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• pp.39-44
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• 2007

Purpose: To evaluate the results of subtalar arthroscopy and to define the indications for the procedure. Materials and Methods: Fifty-four patients were followed up for more than 1 year after subtalar arthroscopy. The mean age was 40 years, and the mean follow-up period was 18 months. Preoperative diagnoses included sinus tarsi syndrome in 19 cases, degenerative arthritis in 13 cases, calcaneal fracture in 10 cases, arthrofibrosis in 5 cases, os trigonum syndrome in 3 cases, talar fracture in 3 cases, talocalcaneal coalition in 3 cases and calcaneal tumor in 1 case. Clinically AOFAS ankle-hindfoot scale and satisfaction of the patients were evaluated. Results: There were 23 synovectomies, 13 subtalar fusions, 11 diagnostic arthroscopies, 5 adhesiolyses, 4 loose body removals, 3 excisions of os trigonum and 1 arthroscopic reduction of fracture. Twenty-five ankle arthroscopies and 11 modified Brostrom's operations were performed for the accompaning 17 ankle impingment syndromes, 11 chronic ankle instabilities and 7 osteochondral lesions of talus. AOFAS ankle-hindfoot scale was increased from 33 points preoperatively to 77 points postoperatively in subtalar fusion group, and was increased from 69 points preoperatively to 89 points postoperatively in other-than-fusion group. Ninety one percent of patients were satisfied with the procedures. There were no serious complications related to the subtalar arthroscopy. Conclusion: Subtalar arthroscopy appears to be safe and highly accurate procedure for subtalar pathology, although it requires technical expertise.