• Journal of Applied Biological Chemistry
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• v.66
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• pp.197-203
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• 2023

Callus cultivation is a method for producing a large amount of tissue of a plant in the laboratory, regardless of the environment. Lycoris chejuensis, a plant species native to jeju island, is a member of the Lycoris family has been used as a traditional medicine for the treatment of diverse diseases. In this study, we evaluated anti-inflammatory effect of biorenovated Lycoris chejuensis callus (LCB) in lipopolysaccharide (LPS)-induced RAW264.7 cells. As a result, LCB was less toxic to the cells in the concentration range of 25, 50, and 100 ㎍/mL as shown by the improved viability of LCB treated cells than compared to Lycoris chejuensis callus (LC) treatment. In addition, LCB inhibited the generation of NO and prostaglandin E₂ through the suppression of inducible nitric oxide synthase and cyclooxygenase-2 protein expression. LCB also attenuated the expression of interleukin-1β, interleukin-6 and tumor necrosis factor-α induced by LPS. The results suggest that LCB has anti-inflammatory activity on the LPS-induced inflammatory response and may be suitable for the development of potent functional cosmetic material.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.44-44
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• 2018

경상북도 봉화군에 위치한 각화산(1,177m)은 태백산맥에서 소백산맥으로 갈라지는 기점을 이루는 산으로 구령산, 청옥산, 옥석산 등과 함께 백두대간 일대의 중요한 산으로 인식된다. 특히 각화산은 조선왕조실록을 보관했던 태백산 사고지터(사적 제 348호)를 품고 있는 역사적인 장소이기도 하다. 본 연구는 각화산 일대 관속식물의 분포상을 파악하고자 2017년 5월부터 2018년 9월까지 진행되었다. 연구 결과 조사지 내 관속식물은 92과 291속 413종 4아종 38변종 6품종으로 총 461분류군이 확인되었다. 이는 경상북도 관속식물 1,684분류군(Korea National Arboretum, 2016)의 27.4%, 한반도 관속식물 4,499분류군(Korea National Arboretum, 2018; Http://www.nature.go.kr/kpni/)의 10.2%에 해당되는 것으로 분석되었다. 각화산 일대의 주요 식물 가운데 한국특산식물은 12분류군(할미밀망, 진범, 처녀치마, 지리대사초 등)이 확인되었고, 산림청 지정 희귀식물은 VU등급(꼬리진달래, 노랑무늬붓꽃) 2분류군, LC등급(너도바람꽃, 쥐방울덩굴, 도깨비부채) 3분류군으로 총 5분류군의 희귀식물이 분포하는 것으로 조사되었다. 또한, 식물구계학적 특정식물 IV등급 5분류군, III등급 13분류군, II등급 21분류군, I 등급 25분류군이 확인되었고, 외래식물은 총 24분류군으로 도시화지수(%) 6.8%, 귀화율은 5.2%에 해당되는 것으로 분석되었다. 조사된 식물의 유용성 분석 결과, 식용식물 348분류군(75.5%), 섬유용식물 1분류군(0.2%), 약용식물 기분류군(15.4%), 관상용식물 18분류군(3.9%), 목초용식물 102분류군(22.1%) 목재용식물 14분류군(3.0%) 등으로 확인되었다. 본 연구 결과물은 각화산의 식물 현황을 제시함과 동시에 생태축으로 연결된 백두대간 일대의 효율적인 관리 및 보전 방안을 마련하기 위한 기초자료로 활용 될 것이라 기대한다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.45-45
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• 2018

경상북도 봉화군에 위치한 갈방산(712m)은 백두대간 옥석산(1,244m)에서 분기한 문수지맥의 산지 가운데 하나다. 본 연구는 갈방산 일대 관속식물의 분포상을 파악하고자 2017년 5월부터 2018년 9월까지 진행되었다. 연구 결과 92과 265속 373종 4아종 44변종 5품종으로 총 426분류군의 관속식물이 조사지에서 확인되었다. 이는 경상북도 관속식물 1,684분류군(Korea National Arboretum, 2016)의 25.3%, 한반도 관속식물 4,499분류군(Korea National Arboretum, 2018; Http://www.nature.go.kr/kpni/)의 9.5%에 해당되는 것으로 분석되었다. 갈방산 일대의 주요 식물 가운데 한국특산식물은 11분류군(키버들, 병꽃나무, 처녀치마 등)이 확인되었고, 산림청 지정 희귀식물은 VU등급(세잎승마) 1분류군, LC등급(도깨비부채, 태백제비꽃) 2분류군으로 총 3분류군의 희귀식물이 분포하는 것으로 조사되었다. 또한, 식물구계학적 특정식물 V등급 1분류군, IV등급 2분류군, III등급 10분류군, II등급 16분류군, I 등급 23분류군이 확인되었고, 외래식물은 총 22분류군으로 도시화지수(%) 6.3%, 귀화율은 5.2%에 해당되는 것으로 분석되었다. 조사된 식물의 유용성 분석 결과, 식용식물 345분류군(81%), 섬유용식물 4분류군(0.9%), 약용식물 85분류군(20.0%), 관상용식물 18분류군(4.2%), 목초용식물 109분류군(25.6%) 목재용식물 15분류군(3.5%) 등으로 확인되었다. 요컨대 갈방산 일대의 식물 현황을 제시함으로써 본 연구 결과물이 갈방산 일대의 희귀특산식물과 유용식물의 보전 및 활용 방안을 마련하기 위한 기초자료로 활용 될 것이라 기대한다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.25-25
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• 2018

본 연구는 경상북도 봉화군에 위치한 문수산 일대의 관속식물상을 파악하고자 수행되었다. 2010년 3월부터 2017년 11월까지 계절별 출현식물을 대상으로 총 12회에 걸쳐 조사를 실시하였다. 조사지 내 관속식물은 85과 241속 372종 5아종 58변종 7품종으로 총 442분류군이 분포하는 것으로 조사되었으며, 이는 경상북도 관속식물 1,684분류군(Korea National Arboretum, 2016)의 26.2%, 한반도 관속식물 4,499 분류군(Korea National Arboretum, 2018; Http://www.nature.go.kr/kpni/)의 9.8%에 해당되는 것으로 분석되었다. 문수산 일대의 한국특산식물은 12분류군(세잎승마, 할미밀망, 은꿩의다리, 노랑갈퀴, 고려엉겅퀴 등), 산림청 지정 희귀식물은 VU등급(주목, 백작약, 꼬리진달래 등) 5분류군, LC등급(쥐방울덩굴, 도깨비부채, 태백제비꽃, 꽃개회나무 등) 10분류군으로 총 15분류군이 분포하는 것으로 조사되었다. 또한, 식물구계학적 특정식물 V등급 2분류군, IV등급 5분류군, III등급 16분류군, II등급 25분류군, I등급 30분류군, 외래식물은 서양벌노랑이, 토끼풀, 달맞이꽃, 미국가막사리, 개망초, 주걱개망초, 털별꽃아재비, 서양민들레, 오리새, 큰김의털 등 10분류군으로 도시화지수(%) 3.1%, 귀화율은 2.3%에 해당되는 것으로 조사되었다. 조사된 식물을 용도별로 구분하면 식용식물 338분류군(76.5%), 섬유용식물 3분류군(0.7%), 약용식물 75분류군(17.0%), 관상용식물 21분류군(4.8%), 목초용식물 95분류군(21.5%) 목재용식물 16분류군(3.6%) 등으로 분석되었다. 본 연구가 봉화군 문수산 일대의 효율적인 관리 및 보전 방안을 마련하기 위한 기초자료로 활용 될 것이라 기대한다.

• Journal of Chest Surgery
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• v.41 no.5
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• pp.573-579
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• 2008

Background: Composite valve graft replacement is currently the treatment of choice for a wide variety of the lesions of aortic root disease. The purpose of this study was to explore the results of aortic root replacement after using the Cabrol technique over a 13-year period at our institution, and we analyzed the results to help surgeons make better decisions when repairing aortic root disease. Material and Method: Between January 1994 and December 2006, twenty-five patients underwent a Cabrol technique operation at our institution. The mean patient age was $43.7{\pm}14.1$ years old (range: $6{\sim}65$ years) and the male and female ratio was 21:4 (84% : 16%). The patients' follow-up was 100% complete, and the mean follow-up period was $60.7{\pm}50.4$ (range:$1{\sim}162$) months. Annuloaortic ectasia (n=18) was the most frequent cause of aortic disease in this series, followed by aortic dissection (n=7). The mean cardiopulmonary bypass time was $177.2{\pm}44.9$ minutes and the mean aortic cross clamping time was $123.4{\pm}34.1$ minutes. Nine patients were checked with MDCT (Multidetector computed tomography) for evaluating a well functioning secondary graft and the coronary anastomosis site. Result: The early mortality rate was 4% (1 of 25 patients). A significant stenosis, kinking or occlusion of the secondary graft was detected by MDCT in 4 patients. The overall survival rate was 88%. Conclusion: The Cabrol technique demonstrated a significant incidence of long-term complications such as secondary graft stenosis or obstruction. It could be used when the modified Bentall technique is not feasible.

• Journal of Chest Surgery
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• v.41 no.5
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• pp.563-567
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• 2008

Background: The histomorphological properties of the left anterior desecending artery (LAD), the left internal thoracic arteries (LITA), the radial arteries (RA) and the intercostal arteries (ICA) were studied for their use as a conduit for coronary artery bypass grafting (CABG), and we compared them with each other. Material and Method: All the vessels were harvested from nineteen cadavers (17 males and 2 females). The mid-portion of the LAD, the mid-portion of the LITA, the distal RA and the mid-portion of the 5th ICA were obtained. All of them were stained with hematoxylin-eosin and with Van Gieson's elastin stain. The morphological characteristics were examined and the thicknesses of the intima and media (I/M ratio: the intima to media ratio) were compared using one-way ANOVA tests. Result: The mean age of the cadavers was $61.5{\pm}9.6$ years. The LITA and ICA were elastic arteries, and the LAD and RA were muscular arteries. The I/M ratio showed statistically significant differences: $0.07{\pm}0.03$ in the LITA, $0.16{\pm}0.11$ in the ICA, $0.45{\pm}0.29$ in the RA and $0.93{\pm}0.52$ in the LAD, respectively. Conclusion: This study showed that the degrees of intimal hyperplasia of the CA and the various conduits for CABG were different significantly. The ICA was found to have relatively favorable characteristics as a coronary by pass conduit, but its suitability for clinical use is a challenging issue.

• Journal of the Korean Institute of Landscape Architecture
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• v.46 no.1
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• pp.96-105
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• 2018

This study examined the growth of herb plants in response to irrigation on a green rooftop area in order to select herb plants that can be used for rooftop greening. Apple Mint (Mentha suaveolens), Lemon balm (Melissa officinalis), Spearmint (Mentha spicata), Pineapple sage (Salvia elegans), Choco Mint (Mentha ${\times}$ piperita 'Choco Mint'), Ox-eye Daisy (Chrysanthemum leucanthemum), Roman Chamomile (Anthemis nobilis) and Thyme (Thymus vulgaris) showed increased growth when irrigated. Conversely, Lavender (Lavendula angustifolia ), Peppermint (Mentha ${\times}$ piperita ), Vicks Plant (Plectranthus tomentosa), Feverfew (Tanacetum parthenium), Rosemary (Rosmarinus officinalis), Tansy (Tanacetum vulgare), Lemon Verbena (Aloysia triphylla), Heliotrope (Heliotropium arborescens), Soapwort (Saponaria officinalis) and Lady's mantle (Alchemilla vulgaris) demonstrated satisfactory growth regardless of irrigation. Peppermint, Tansy, Lemon Verbena, Soapwort, and Lady's mantle seem to be suitable for green rooftop because of their overwintering ability and drought hardiness. Pineapple sage, Apple Mint and Thyme would seem to be inappropriate for rooftop greening because they showed negative growth response to drought and failed overwintering. Although Spearmint, Lemon balm, Choco Mint, Ox-eye Daisy and Roman Chamomile had reduced growth during dry conditions, they were able to overwinter satisfactorily and can be used as rooftop plants with irrigation.

• Horticultural Science & Technology
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• v.17 no.4
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• pp.489-493
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• 1999

This study was conducted to investigate the potentiality of various Korean Sedum species as ornamental plants based on morphological characteristics and to analyze the genetic relationship among the Sedum species. S. kamtschaticum and S. takesimense possessing splendour flowercluster with yellow color could be suggested for garden plant, S. routundifolium having pink flower-clusters with round leaf shape for pot flower or garden plant and S. sarmentosum, S. polystichoides and S. oryzifolium with creeping stem and low plant height for ground cover plant or floral carpet. Eighteen oligonucleotide random primers were used to amplify genomic DNA of Sedum species using polymerase chain reaction (PCR). Ninety five polymorphic bands among 125 different DNA fragments in the range of 224 to 3,675 base pairs were obtained from RAPD analysis. Similarity matrix of RAPD profiles was generated by coefficient value of variation, and the data were subjected to be cluster analysis. Fifteen lines of Sedum species analyzed were classified into 3 groups with the similarity coefficient value of 0.418, and 12 groups with the value of 0.328. RAPD results showed similar trends as the morphological characteristics of the plants.

• Journal of the Korean Institute of Landscape Architecture
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• v.36 no.6
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• pp.34-42
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• 2009

This study was executed to introduce a greenhouse style "Evergreen Park" to apartment complexes to target hands-on resident participation and application rather than a mere viewing of the plants as a way to provide convenient and profitable service to residents in a year-round center of usable outside space. The four key points can be summarized as follows: first, subtropical plants are evergreen broad-leaved trees, which maintain their green during all four seasons the leaves are thick and glossy. Greenhouses geared toward these subtropical trees-mainly broad-leaved evergreen species-are in planning to introduce these unique, elegant plants to temperate regions. Residentswill not only gain an education regarding these species but will be provided with the best quality evergreens at very reasonable maintenance costs. Second, subtropical plants greenhouses introduced in apartment complexes are suggested for structures connected via underground passage as well as free-standing structures so as to make use of geothermal heating and apply to reducing sunlighting. Third, as a way to provide (1) health & relaxation(evergreens, herb garden, water space), (2) community & education (plant flea market/plant hospital, plant-related lecture(exhibition), hands-on experience program), (3) a vine garden for year-round use such festivities as a Butterfly Festival, Aroma festival, Smile Oak Nuts, Candlelight Festival and Christmas Photo Site. Lastly, it has been suggested that the operation and maintenance of these greenhouses will be both by resident council operation management and by outsourcing company operation management.

• Korean Journal of Food Science and Technology
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• v.47 no.3
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• pp.321-327
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• 2015

The optimal condition for preparation of powdered calcium acetate (LCCA) which has high solubility, from calcined powder (LCCP) of the littleneck clam shell by response surface methodology (RSM) was examined. Increased molar ratio of LCCP led to reduced solubility, yield, color values, and overall quality. The critical values of multiple response optimization of independent variables were 2.57 M of acetic acid and 1.57 M of LCCP. The actual values (pH 7.0, 96.1% for solubility, and 220.9% for yield) under the optimized condition were similar to the predicted values. LCCA showed strong buffering capacity between pH 4.89 and 4.92 on addition of ~2 mL of 1 N HCl. The calcium content and solubility of LCCA were 21.9-23.0 g/100 g and 96.1-100.1%, respectively. The FT-IR and XRD patterns of LCCA were identified as calcium acetate monohydrate, and FESEM images revealed an irregular and rod-like microstructure.