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Establishment of an Analytical Method for Novobiocin in Livestock Products Using HPLC-UVD (HPLC-UVD를 이용한 축산식품 중 Novobiocin의 시험법 확립)

  • Park, Hee-Ra;Kwon, Chan-Hyeok;Lee, Jong-Goo;Kim, Hyung-Soo;Chae, Young-Sik;Oh, Jae-Ho;Kwon, Ki-Sung
    • Korean Journal of Food Science and Technology
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    • v.44 no.3
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    • pp.263-268
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    • 2012
  • Novobiocin is a coumarin-containing antibiotic, and has a longer half-life in various animals than other veterinary medicines. A simple and rapid high-performance liquid chromatography assay for the determination of residual novobiocin levels in chicken, beef and milk has been developed and validated. The separation condition for HPLC/UVD was optimized by a MG II $C_{18}$ (4.6 mm $ID{\times}250$ mm, 5 ${\mu}m$) column with 0.1% formic acid in $H_2O$/0.1% formic acid in Acetonitrile (40/60, v/v) as the mobile phase at a flow rate of 1.0 mL/min and the detection wavelength was set at 340 nm. Residues were extracted from tissue by blending with methanol. After liquid-liquid partitioning, lipid materials were removed with n-hexane and purification as Silica (1 g, 6 mL) cartridge with 10 mL acetone/dichloromethane (10/90, v/v). Limit of quantification and linearity performed by the analytical method were 0.02 mg/kg and 0.999 ($r^2$), and the recovery range was $88.8{\pm}5.6-100.3{\pm}4.4$, $88.8{\pm}7.2-97.0{\pm}3.2$ and $88.1{\pm}4.3-92.8{\pm}3.6%$. It is expected that this analytical method with regards to novobiocin in chicken, beef and milk could be applied as an official method to administer food safety on veterinary medicines.

Independent Production Routines and Environmental Changes In 'Comprehensive Programming Television Channels' in Korea Focusing on Interviews with Independent Producers, Broadcast Writers and Individuals Involved with the TV Channels (종합편성채널의 독립제작 환경과 관행에 관한 연구 독립PD, 작가 및 종합편성채널 관계자 심층인터뷰를 중심으로)

  • Choi, Sun Young;Han, Hee Jeong
    • Korean journal of communication and information
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    • v.73
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    • pp.56-91
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    • 2015
  • This study examined changes in the independent production environment in the perspectives from flexible specialization of labor and media routines since January 2011, when comprehensive programming television channels (JTBC, MBN, Channel A, TV Chosun) emerged in Korea. In-depth interviews were conducted with thirteen individuals, including producers from independent production companies, broadcast writers, and individuals involved with these TV channels. The interview results indicated that a flexible specialization production system had been established by the comprehensive programming channels. This means that they were heavily dependent on independent producers, except in relations to their own news programs. Moreover, it was identified that the production of diverse programs could be difficult due to absurd contract practices such as those related to TV ratings and performance systems. Second, these channels have implemented some positive changes such as the payment of higher production costs and an incentive system, compared to terrestrial TV stations. However, the incentive system also helps to aggravate internal competition in the channel and also instigate contract competitions among independent companies, which can eventually result in the channels for holding exclusive rights to certain content and, hence, unfair business practices. Third, as a result of the newspaper and broadcast cross-owenership system of the comprehensive programming channels, hierarchical independent production practices can be established under the influence of newspaper proprietors and executives or managers who have previously worked for newspapers. Lastly, as a result of interviews with independent producers and individuals involved with the TV channels concerning the awareness of comprehensive programming channels, it could not be ascertained whether it is difficult to produce programs dealing with diverse items and genres, because programming autonomy has been distorted by capital or the advertisement market. In this circumstance, it is not surprising that some comprehensive programming channels mentioned that they prioritize profit and performance in programming. In conclusion, it is absolutely imperative that complementary and legal measures be implemented institutionally in order to redress the existing systematic dysfunctional routines in the independent productions of the comprehensive programming TV channels in Korea.

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Assessment of Pollutant Loads from Alpine Agricultural Practices in Nakdong River Basin (낙동강 수계 고령지 밭의 비점오염 물질 유출 특성 조사 및 단위 유출량 산정)

  • Joo, Jin-Ho;Yang, Jae-E.;Ok, Yong-Sik;Oh, Sang-Eun;Yoo, Kyung-Yeol;Yang, Su-Chan;Jung, Yeong-Sang
    • Korean Journal of Environmental Agriculture
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    • v.26 no.3
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    • pp.233-238
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    • 2007
  • To assess pollutant loads in Nakdong river from highland agriculture in Kyungbuk province we. analyzed water qualities such as BOD, COD, T-N, T-P and SS in year 2005. BOD values in rainy period (June and July) were relatively higher than those in dry period, and those in 4 sites among 17 sites ranged from 10.71-19.25 mg/L which exceeded water criteria (8 mg/L) for agricultural use. COD values showed similar trends like BOD values. These trends might be caused by outflow of nutrients applied in agricultural lands. T-N content ranged from 0.1 to 14 mg/L. Those in lower reaches of stream were greater in those in upper stream. Compared to T-N contents during non-farming season, T-N content in farming season were higher. These phenomenon could be due to continuous input of nutrients from small watercourses. Averaged T-P content in lower stream during farming season was 0.4 mg/L, which was eight times higher than the limiting level for algae occurrence (0.05 mg/L). BOD, T-P, T-N loads from alpine agricultural practices were 12.25 $kg/km^2{\cdot}day$, 0.55 $kg/km^2{\cdot}day$ and 32.35 $kg/km^2{\cdot}day$, respectively. These values were greater than those from forestry. Therefore, Best management Practices (BMP) for alpine agricultural field are needed to reduce pollutant loads in Nakdong river.

Evaluation of the Effect of Operation of Toothbrushing Room in between Two Elementary Schools (일부 초등학교 양치교실 운영 효과 평가)

  • Seong, Mi-Gyung;Kwun, Hyeon-Sook;Moon, Sook-Ryeon;Ryu, Hae-Gyum
    • Journal of dental hygiene science
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    • v.15 no.1
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    • pp.24-31
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    • 2015
  • This research was conducted in order to examine the effect of tooth brushing room M elementary school in Changwon-city and to provide foundation data for effective project operation afterwards. The subjects were 347 students at the M elementary school where the tooth brushing room was being taught. The control group is 289 students at J elementary school where the tooth brushing room was not being taught. Research and analysis were carried out with structured survey and examination of decayed, missing, filled teeth (DMFT) index, decayed, missing, filled tooth surface (DMFS) index and O'leary index. The data was analysed by IBM SPSS Statistics ver. 19.0 program and the result is as follows: Depends on the tooth brushing room there was difference in statistical significance in filling teeth, sealant tooth surface, filling tooth surface, missing tooth surface, DMFS, O'leary index between the subject and control group. The less the frequency of brushing, the higher the DMFT index. Negative correlation was statistically significant. With incorrect brushing method, the less the frequency, the higher the DMFS index, Negative correlation was statistically significant. When the tooth brushing room was being implemented, O'leary index became low, negative correlation was statistically significant. As a result, in order to continue the effective operation of tooth brushing room, constant supervision and monitoring on students should be acutely needed by a principal, a school nurse and teachers in charge. Also together with a systemized cooperation between a health center and a nearby university's related majors departments, the research proposes to execute constant oral health education and to expand the implementation project of the tooth brushing room at nearby elementary schools.

Nutritional and Health Status of the Elderly Living in Songnam - I. Anthropometric Measurements and Biochemical Nutritional Status - (성남지역 노인의 영양 및 건강상태 조사 I. 신체 계측 및 생화학적 영양상태)

  • 권종숙;이홍재
    • The Korean Journal of Food And Nutrition
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    • v.16 no.4
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    • pp.310-320
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    • 2003
  • To study the nutritional and health status of the elderly in Songnam, the anthropometic measurments, fat composition of body(FAT), blood pressure(BP) and blood test for analyzing biochemical indies of the elderly in seongnam were carried out in 338 free-living elderly (161 men, 177 women) aged from 58 to 92. Anthropometric measurements and blood pressure were assessed, and body fat was analyzed by BI(Bioelectrical Impedence) method. Biochemical measurements including blood glucose(BG), GPT, serum total cholesterol(TC) and albumin(AL) were analyzed. Average heights of the subjects were lower than the standards established in the Korean Recommended Dietary Allowances, and average weights were similar to the standards. FAT were significantly higher in women(36.4%) than men(27.7%), and both groups showed higher values than the reference. Mean systolic BP levels of men and women were 153.3mmHg and 153.7mmHg, and diastolic BP levels were 87.5mmHg and 86.0mmHg, respectively, and there were no significant differences between the sex. Mean BG levels of men and women were 115.9mg/㎗ and 123.7mg/㎗, respectively, and there were no significant differences between the sex and among the age groups. Mean GPT levels of men and women were 19.4unit and 18.1unit, respectively, and women older than 75 years showed significanly low levels of GPT compared to other women's age groups. Mean TC levels of men and women were 186.0mg/㎗ and 206.6mg/㎗, respectively, and significantly higher TC levels were shown in women than men. Mean AL levels of men and women were 4.8g/㎗ and 4.8g/㎗, respectively, and men aged 50 ∼ 64 years showed significant high AL levels compared to other age groups of men. It was suggested that nutrition education program was a good way to improve health status of the elderly.

A Study on the Village Improvement Plan by Typological Analysis of Greenbelt-lifted Villages (개발제한구역 해제취락 유형분석을 통한 취락정비방안 연구)

  • Yoon, Jeong-Joong;Choi, Sang-Hee
    • Land and Housing Review
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    • v.4 no.1
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    • pp.77-87
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    • 2013
  • About 1,800 villages have released from Greenbelt since Greenbelt-reform-policy for readjustment of the area was promoted after 1997. Even though the government intended to attract planned development & improvement of these lifted villages through District Unit Plan and designating the lifted area as low-rise and low-density zoning considering the characteristics of the Greenbelt region, there are still many problems to be solved: a lack of funds, insufficient capability for self-improvement and unexecuted SOCs in long-term etc. It seems that these problems are caused by focusing on the lifting areas itself instead of researching deeply the condition and characteristics of the villages and searching proper direction/plans of improvement before lifting Greenbelt In addition, the existing plan of village improvement and management was not considering physical and spacial characteristics of the areas, social and economic situation of residents and relationship between the villages and surrounding cities, though these conditions are different among each villages, and the related regulations are applied uniformly across all the villages and those have been causing many civil appeals and environmental problems. In these respects, this study aims to consider the problems of the lifted villages using the existing researches on them and to make typology by characteristics-data of the villages and to establish improvement strategies of each types. In this study, the villages were classified into 5 types as a result of cluster analysis on 424 villages among all 1,800 through variables of locational potentiality : location, accessibility, size and form of village, condition of regulations etc. According to function of the villages, they were divided into 4 types: urban-type, rural-type, industrial-type and neighborhood-centered-type. This study also drew 4 improvement-strategy-types by combination of locational potentiality and village-function : type of improving life-environment, type of improving production-infra, type of inducing-planned-improvement and type of constructing center-of living-circle. Finally, this study suggested the directions of the each 4 types to desirable improvement and management which could be used to make and complement plans for village improvement.

Case Study on the Space Characteristics Focused on the Dang and Oreum of the Seashore.Inland Villages in Jeju Island (당(堂)과 오름을 중심으로 한 제주도 해안.중산간마을의 공간 특성 사례연구)

  • Choi, Jai-Ung;Kim, Dong-Yeob;Jo, Lock-Whan;Kim, Mi-Heui;Ahn, Ok-Sun
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.30 no.2
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    • pp.101-109
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    • 2012
  • Traditional village forests in Jeju Island represent unique cultural landscape with a history of more than several hundred years as a national cultural asset in Korea. In this paper, the characteristics and meaning of traditional village forests in Jeju Island was compared with the Dangsan and Bibo forests at inland. There are 368 Oreums, parasitic volcano, and 391 shrines of Dang(Divine place) in Jeju. Life, culture and tradition of rural villages are all connected with the Dang and Oreum in Jeju. It has been found from this study that the village in Jeju were established as a cultural landscape on the surface of natural landscape. The features of traditional villages focused on the Dang and Oreum in Jeju Island were similar to the Dangsan and Bibo forestsat inland villages. The Oreum represents mountain and the Pojedan forest is newly found in Sangmyung-ri. The seashore areas are covered by vaocanic rocks in Jeju and large scale windbreaks are hardly found. The stone tower at Sinheung-ri built for blocking sand movement represents Bibo forest. The special attribute of the Dang in Jeju is that it is close to real life and believers are still remain. In 2009, the Jeju Chilmeoridang Yeongdeunggut ritual was nominated as an Intangible Cultural Heritage of Humanity by UNESCO. The shrine of Dang, however, has been degraded fast by construction of seashore road and Jeju Olle trail path. As for the world cultural heritage discussed at international conferences, it is important that there is sustainability on the right to enjoy cultural heritage. Integrated efforts from local residents, local governments and national government are needed to set up a management scheme for the Dang culture. Rural villages in Jeju with the Dang and Oreum are expected to get an international attention as to have traditional cultural landscapes of Korea.

Effects of Nutrition Education in Type 2 Diabetes Mellitus on Diabetes Control and Blood Antioxidant Status (제2형 당뇨환자에 대한 영양교육이 당뇨병 관리와 혈액 항산화 상태에 미치는 영향)

  • Shin, Kyung-Nam;Lee, Hye-Sang;Kwon, Chong-Suk
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.5
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    • pp.689-695
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    • 2011
  • Diabetic patients need nutritional education more than those suffering from other diseases because of the necessity of controlling blood glucose levels with dietary treatment. The purpose of this study was to find out the effectiveness of nutrition education on diabetes control and antioxidant status, both of which are related to diabetic complications. Thirty (15 males and 15 females) type 2 diabetes mellitus patients aged $66.7{\pm}8.8$ years participated in a 4-week nutrition education program. Nutrient intakes, blood glucose level, antioxidant status, and DNA damage were evaluated before, immediately after, and three months after the education program. Changes in those parameters over time were analyzed using repeated-measures analysis of covariance. Over time, HbA1c (p=0.000), plasma total cholesterol (p=0.002), plasma thiobarbituric acid related substances (TBARS; p=0.000), and leukocyte DNA damage (p=0.000) significantly decreased; plasma retinol (p=0.001), plasma tocopherol (p=0.000), erythrocyte catalase (CAT; p=0.000), and erythrocyte glutathione peroxidase (GPx; p=0.000) significantly increased. In an evaluation of nutrient intakes by Dietary Reference Intakes for Koreans (KDRI), energy (p=0.009), phosphorus (p=0.033), sodium (p=0.001), potassium (p=0.019), zinc (p=0.043), riboflavin (p=0.050), folic acid (p=0.048) and vitamin C (p=0.008) intakes had significant positive changes. In a correlation analysis of the biochemical and nutritional changes resulting from the education program, plasma TBARS were negatively correlated with potassium (r=-0.418, p<0.05), iron (r=-0.443, p<0.05), riboflavin (r=-0.432, p<0.05), and folic acid (r=-0.446, p<0.05) intakes, while plasma retinol was positively correlated with energy (r=0.543, p<0.01), protein (r=0.543, p<0.01), phosphorus (r=0.425, p<0.05), iron (r=0.485, p<0.05), zinc (r=0.570, p<0.01) and niacin (r=0.510, p<0.05) intakes. Erythrocyte CAT was positively correlated with folic acid intake (r=0.605, p<0.01). From these results, we suggest that an improvement in nutrition resulting from a diabetic education program for type 2 diabetes patients led to improvement in their antioxidant status, also possibly reducing complications resulting from diabetes.

A Study on the set the seven roalls of the Saddarma Pundarika Sutra Prayed by Yeom Seong-Ik and Script Center (사경은(寫經院)과 염승익(廉丞益) 발원(發願)의 사경(寫經) 「묘법연화경(妙法蓮華經」 7권본 1부)

  • Kyon, Hee-Kyung
    • Journal of Korean Society of Archives and Records Management
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    • v.3 no.1
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    • pp.1-21
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    • 2003
  • In this paper the characteristics of the script centers golden and/or silver letter center in Koryo Dynasty and a set of seven rolls of the script written in silver letter on blue paper prayed by Yearn Seong-Ik were studied with focusing on the referred records and an epilog of the script. This script was discovered from Namgewon's stone stupa, when it was transported from original site of Daekam Dong, Kaiseong city to Kyungbok Palace in 1919. This script prayed by Yeom Seong-Ik which was written in silver paste on blue papers is a set of seven rolls, the size is 31.1 cm (length) ${\times}$ 27.2 cm (width). The cover painting and Beonsangwha(Buddha's preaching picture) were painted out and in the roll cover. This script was written with 14 letters on a line whose characteristic with the style of cover painting and Beonsangwha is similar to the golden and silver scripts prayed by kings of Koryo Dynasty. The person who prayed this script was a powerful vassal of the king Chungyeol period, Yeom Seong-Ik. According to Koryosa(Koryo History) and Koryosa jeolyo(summarized Koryo History), it is described that Yeom Seong-Ik offered his own house which was built by a group of strangers as a 'Writing Place for the complete collection of Buddhist Sutras and the place of the script of the complete collection of Buddhist Sutras. Therefore, it is possible to consider that this script a set of seven rolls of the script of Saddharma Pundarika-Sutra was written to pray for himself as well as his families' fortune at the place of golden script of the complete collection of Buddhist Sutras, and enshrined it in the Namgaewon stone stupa when the stupa was repaired in 9th year of king ChungYeol. Above all the most important point should be centered on that he offered his house as a writing place for golden script of the complete collection of Buddhist Sutras as recorded in Koryosa Jeolyo. The writing place for golden script of the complete collection of Buddhist Sutras should be emphasized on the meaning of its site. It is an important key point to clear that Kumjawon(Golden letter center) would be different from Kumjadejangso(the writing place of golden script for complete collection of Budihist Sutras) with Kumkyungsa(Goldm script place). As considereing the point that this script showed the characteristics of golden and/or silver scripts which prayed by king Chungyeol in Koryo Dynasty as they were, even though this script was manufactured by individual praying, this script follwed in the style of Kumjawon(golden letter center) and/or Unjawon(siIver letter center) as they were, because this script was written in the place of the golden script for the complete collection of Buddhist Sutras. In this paper all of the points described above with focusing on the referred records and an epilog of this script were collectively considered to make clear the characteristics of the script centers, golden and/or silver letter.

Influence for a state of nutrition reached over Hb formation (영양상태(營養狀態)가 Hb 형성(形成)에 미치는 영향(影響))

  • Lee, Geum-Yeong;Jeon, Kwan-Bae
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.4 no.1
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    • pp.13-17
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    • 1975
  • 1. A nursery school principal must think over physical stamina and health of his children, and he must make an epoch making changes to nutrition control. I think that authorities concerned must give weight to quality before quantity to manage the nursery school. If the nursery school principal who has aid received from abroad and his native country must manage the nursery school rationally. It is expected that, though the nutrition level is below the level of sports middle school children, it is above the common level of home children. 2. I fear that the calories which the pupils of sports middle school assimilate a day on the average is apt to be more calories as compared with other pupils of their age(weight). This is also true for their level of activity. It is required that the nutritionist must recheck the quantity of the calorie provision. 3. I took no thought about infection with parasites in this thesis. It is because that the pupils of sports middle school have much Hb (hemoglobin), because they have had meals with a settled menu for a year or two. So, that it is expected that it is caused only by the nutritious foods regardless of parasites and that they have little Hb(hemoglobin). Besides though they are little pupils, because they have much Hb. Of taking the level with upper life classes of the the Ewha Girls University students, it will be more reliable. Therefore as we maintain the states of health of the common home children and nursery school children normally, to increase the national power, the managers of nursery schools or the heads of families must pay attention to the control of nutrition, and authorities concerned must pay attention to the control of nutrition, and authorities concerned must take the trouble much more to the management of nursery school.1. We have been undertaking the occupations of the family plannings for more ten years. But we have still failed to realize the anticipated result in the farm villages. Though it is not in this district, I think that we must make efforts in these occupations urgently, and control increases in population, decrease the consumptions of food and salt, and contribute to the increasing of the general income. 2. There is a tendency to take a little more salted foods in the cities than in the farm villages. But we are apt to take more salt in quantity(20g) to be taken in a day both in the cities and in the farm villages than it is necessary to us. So it is required that we must grope for a means of increasing of the general income as early as possible, and that we must improve our diet to be got rid of dietary life in importance. And so we have to study throughly the side effect caused due to too much salt to be taken in, if it is discovered, we must spour on a means of sweep of it.

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