To select the effective fungicides for the control of leaf spot disease of jujube tree (Zizyphus jujuba) caused by Phoma sp., inhibitory effects of 26 fungicides for mycelial growth were investigated at $250{\mu}g\;a.i./m{\ell}$. In the test, eight fungicides were selected and minimum inhibitory concentration (MIC) for mycelial growth and an inhibitory effect for spore germination were investigated. Among the fungicides, myclobutanil, hexaconazole, and triflumizole were excluded in control effect tests because of their relatively high MICs. MICs were ranged $10-50{\mu}g\;a.i./m{\ell}$ for benomyl, carbendazim + kasugamycin (CK), and thiophanate-methyl. triflumizole (TT), and $50-250{\mu}g\;a.i./m{\ell}$ for iprodione + propineb (IT) and iminoctadine-triacelate (IT). However, benomyl and IP showed very low inhibitory effect on conidial germination. When the fungicides were sprayed on the seedlings before the leaves were inoculated with conidial suspension of Phoma sp., the protective values of CK and TT were around 70% at 1,000 ppm and around 90% at 2,000 ppm. The protective values were around 70% at 2,000 ppm (benomyl), 4,000 ppm (IP), and 8,000 ppm (IT). When the fungicides were sprayed after inoculation, benomyl showed the highest curative values of over 90% at 1,000 ppm and the values of CK and TT ranged $70{\sim}80%$ at 1,000 ppm. However, IP and IT had little or no effect on therapy of the disease. IT caused necrotic phytotoxicity on the leaves of jujube seedlings. As results, the best fungicides for the protection of jujube trees from leaf spot disease were CK (2,000 ppm) and TT (2,000 ppm) and for the remedy of the tree, benomyl (1,000 ppm) was the best. Therefore, alternate application of benomyl and CK or TT will be effective in the disease control.
In order to control garlic white rot (Sclerotium cepivorum), which threatens garlic production in farmers fields, soil solarization (solar sterilization), sclerotia germination inducers and effective microorganisms as biological control agents, and chemical fungicides have been used. Among them, fungicide has been largely used to reduce garlic white rot. In this study, the antifungal activities of five fungicides, prochloraz(a.i. 25%, EC), tebuconazole (a.i. 25%, WP), flutolanil (a.i. 15%, EC), iminoctadine tris-albesilate (a.i. 40%, WP) and isoprothiolane (a.i. 40%, EC) with different mode of action, in mycelial growth, sclerotia germination and sclerotia production, were tested. The inhibitory effects of the 5 fungicides on the mycelial growth, and sclerotia germination and production of garlic white rot pathogen (S. cepivorum T11-2) were investigated on potato dextrose agar (PDA) and their control efficacies were evaluated on garlic flakes. There was no mycelial growth of S. cepivorum T11-2 on PDA amended with $0.8{\mu}g\;mL^{-1}$ of prochloraz or $100{\mu}g\;mL^{-1}$ of tebuconazole. Also prochloraz and tebuconazole inhibited perfectively the sclerotia germination of the pathogen at 10 and $1.0{\mu}g\;mL^{-1}$, respectively. In spite of a very low activity of isoprothiolane in mycelial growth and sclerotia germination of S. cepivorum T11-2, it showed a good inhibitory activity against sclerotia production of S. cepivorum T11-2 on PDA amended with $1.67{\mu}g\;mL^{-1}$. Prochloraz, tebuconazole and flutolanil showed above 70% of control value when they were treated at $100{\mu}g\;mL^{-1}$ using the garlic flake cutting-method.
This study was implemented to evaluate food safety on residual pesticides in agricultural products of Korea and to use as a data base for the establishment of food policy. A total of 196 pesticide upon these products were analyzed using multi class pesticide multiresidue methods of Korean Food Code, and 232 samples of 15 agricultural products collected from 9 regions were supplied for this study. In the results, 64 kinds of pesticides were detected in 53 samples, chlorpyrifos and procymidone of them were shown a high frequency of detection in the analyzed pesticides. Among them, two samples (chlorpyrifos in perilla leaves and picoxystrobin in peach) were detected over Maximum Residue Limits (MRLs). The levels of the detected pesticide residues were within safe levels. Also, the intake assessment for pesticide residues including chlorpyrifos at multi pesticide residue monitoring were carried out. The result showed that the ratio of EDI (estimated daily intake) to ADI (acceptable daily intake) was 0.001~0.902% which means that the detected pesticide residues were in a safe range so that residual pesticides in the agricultural products in Korea are properly controlled.
In order to elucidate the residual characteristics of some pyrethroid insecticides commonly used for Korean cabbage, such as bifentbrin, lambda-cyhalothrin and deltametbrin, the test pesticides were sprayed onto the crop at recommended rate and doubled rate of the recommendation. Their detection limits were 0.004 mg $kg^{-1}$ and mean recoveries at the fortification levels of 0.04 and 0.2 mg $kg^{-1}$ were from 95.16 to 99.32 and from 86.81 to 103.73%, respectively. Half-lives were from 2.5 to 3.6 at the recommended rate and from 2.3 to 3.9 days at the doubled rate of the recommendation. Initial residue amounts of bifenthrin and lambda-cyhalothrin at the recommended rate and doubled rate of recommendation and of deltamethrin at the recommended rate were less than their MRL, whereas, in case of deltamethrin sprayed at doubled rate of the recommendation, the residue level exceeded its MRL. However, the residue levels of the pesticides in the crop sampled at harvest were less than their maximum residue levels and the ratios of the estimated daily intake (EDI) to acceptable daily intake (ADI) for the pesticides tested calculated from the residue data at harvest were less than 7%, suggesting that these pesticides would be safe for Korean cabbage from the residue concern.
Characteristics of the 5 biopesticides that included Bacillus thuringiensis and on the domestic markets were investigated. These products were contained different strains of B. thuringiensis, for examples; product A and E was B. thuringiensis subsp aizawai; product B was B. thuringiensis; product C was B. thuringiensis Berline var. kurstaki; product D was B. thuringiensis var. kurstaki. Number of active spores were counted because they could influence the bio-activity against target pests. Only product C are contained the fixed quantity as its label, however, product D and E were a tenth part, and product A and B were a hundredth part of their descriptions. The pHs of product A and B were measured 3.67 and 3.73, and C, D and E were 5, respectively. Typical bypyramidal crystals produced from B. thuringiensis was found in only product C under a phase contrast microscope. For the uniform formulation of products that conformed whether B. thuringiensis were equally spreaded on the crops, B. thuringiensis in the C, D and E were equally grown on the nutrient agar medium As a results, product A were more different from product C than any other products. When product A and C were bioassayed against different larval stages of diamondback moth, their mortalities with spraying application were showed 100% after 48 hours.
Kim, Yong-Ki;Hong, Sung-Jun;Jee, Hyung-Jin;Shim, Chang-Kee;Park, Jong-Ho;Han, Eun-Jung;An, Nan-Hee;Lee, Seong-Don;Yoo, Jae-Hong
The Korean Journal of Pesticide Science
/
v.15
no.1
/
pp.55-60
/
2011
To work out quality control methods of environmental-friendly organic materials (EFOMs), the reason and basis for EFOM-selection and farmer's favorite formulation type of EFOMs, etc were investigated on farmers who had been practicing environmental-friendly agriculture. EFOMs used were soil amendments, control agents of plant diseases and insect pests, plant growth promotion formulations, in turns. In EFOMs application time, 22.7% of farmers sprayed EFOMs without delay after they were bought, in other hand, 77.3% of farmers used EFOMs which had been bought and stored for some period. Microbial density on seventeen environmental-friendly microbial formulates (EFMFs) including microbial pesticides, a microbial fertilizer, and environmental-friendly organic materials was investigated at different storing temperature and shelf life. When the microbial density of EFMFs was investigated without delay after they were bought, all used microbial pesticides and a microbial fertilizer was confirmed to be optimal for the certified density but two of environmental-friendly organic materials was confirmed not to be optimal. When microbial density of 17 EFMFs were investigated after storing them for six months at $4^{\circ}C$, only one of 9 microbial pesticides was confirmed not to be optimal, the other hand four of seven environmental-friendly organic materials not to be optimal, which each of their microbial density was less than the certified density. Population dynamics of microbial agents was much more influenced in fluctuated temperature (room temperature) than in static temperature condition ($5^{\circ}C$ and $25^{\circ}C$). Shelf life of microbial agents according to microbial formulation type were high in granule type, liquid wettable type and liquid type in turns.
Although Umboniunm thomasi is one of marine mollusc (Archaeogastropoda: Trochidae) inhabiting the sands in the intertidal zone of the west coast of Korea, aspects of its reproductive biology are still not too well known. Reproductive cycle, gametogenesis, and first sexual maturity of U. thomasi collected at the west coast of Buan-gun, Jeollabuk-do, Korea were investigated monthly from January to December 1999. U. thomasi was dioecious, and an oviparous. The gonad was placed in the rear of the flesh part in the spiral shell. The external colors of the ripe ovary and testis appeared to be green and milk-white or yellowish white, respectively. Meat weigh rate peaked in July ($37.5\%$). And then the value sharply decreased in September ($28.3\%$), thereafter, gradually increased in November ($31.7\%$). Fully ripe oocytes were approximately 100$\~$110 $/mu$m in diameter, and their cytoplasm contained a great number of yolk Branules. Based on the monthly changes of the Bonadal development, gametogenesis, and meat weight rate, the reproductive cycle of U. thomasi could be devided into five successive stages: early active (November to April), late active (February to May), ripe (April to August), spawning (July to October), and recovery (September to February). Gonadal development and spawning were closely related to the seawater temperature, the main spawning occurred in September when the temperature reached above 24.2$^{\circ}C$. Individuals of 4.4 mm and less in shell height could not take part in reproduction in both sexes. Percentages of first sexual maturity of female and male shells ranging from 5.5 to 6.4 mm were $55.0\%$ and $61.9\%$, respectively, and $100\%$ of those over 7.5 mm in shell heights in both sexes participated in the reproduction.
In recent years, the security of hardware and software systems is one of the most essential factor of our safe network community. As elliptic Curve Cryptosystems proposed by N. Koblitz and V. Miller independently in 1985, require fewer bits for the same security as the existing cryptosystems, for example RSA, there is a net reduction in cost size, and time. In this thesis, we propose an efficient hardware architecture of underlying field arithmetic processor for Elliptic Curve Cryptosystems, and a very useful method for implementing the architecture, especially multiplicative inverse operator over GF$GF (2^m)$ onto FPGA and futhermore VLSI, where the method is based on optimized unit operation components. We optimize the arithmetic processor for speed so that it has a resonable number of gates to implement. The proposed architecture could be applied to any finite field $F_{2m}$. According to the simulation result, though the number of gates are increased by a factor of 8.8, the multiplication speed We optimize the arithmetic processor for speed so that it has a resonable number of gates to implement. The proposed architecture could be applied to any finite field $F_{2m}$. According to the simulation result, though the number of gates are increased by a factor of 8.8, the multiplication speed and inversion speed has been improved 150 times, 480 times respectively compared with the thesis presented by Sarwono Sutikno et al. [7]. The designed underlying arithmetic processor can be also applied for implementing other crypto-processor and various finite field applications.
Journal of the Korean Society of Food Science and Nutrition
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v.37
no.4
/
pp.437-444
/
2008
Anti-obesity effects of ginseng and herbal plant mixtures were investigated to develop natural materials for anti-obesity. After inducing obesity with high fat diet for 8 weeks in male SD rats, ginseng and herbal plant mixtures DM1 (ginseng, puer tea, opuntia) and DM2 (ginseng, puer tea) were administrated orally to rats for another 8 weeks. During administration, food efficacy ratio and body weight of rat were measured twice weekly. After administration, body weight, body fat contents, and serum lipid level were estimated for anti-obesity effect and hematological analysis blood level of ALP and ASP was checked for safety. Body weight in rats fed high fat diet was significantly increased. Body weight in obese rats induced by high fat diet was significantly decreased by DM1 and DM2 feedings. The amount of body fat (epididymal, perirenal and visceral fat, brown adipose tissue) was significantly reduced by DM1 and DM2 treatments. The amount of TG, the concentration of leptin in blood plasma, and the concentration of insulin in blood plasma were significantly diminished by DM1 and DM2. Lipid accumulation on liver was reduced in DM2. There were no side effects among all groups according to blood analysis, hematological findings, and body weight. The findings of this study suggest that DM1 and DM2 may be effective materials for anti-obesity through reducing plasma triglyceride and body fats, and also decreasing body weight without side effects.
Ham, Seung-Shi;Kim, Soo-Hyun;Yoo, Su-Jong;Oh, Hyun-Taek;Choi, Hyun-Jin;Chung, Mi-Ja
Journal of the Korean Society of Food Science and Nutrition
/
v.37
no.4
/
pp.416-421
/
2008
This study was performed to determine the antimutagenic and anticytotoxic effects of soybean paste (doenjang) added deep sea water salt and see tangle in Salmonella Typhimurium TA98, TA100 and human cancer cell lines. In the Ames test, methanol extract of doenjang did not exhibit any mutagenicity but showed substantial inhibitory effects against mutation induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and 4-nitroquinoline-1-oxide (4NQO). The methanol extracts of doenjang ($200{\mu}g$/plate) added deep sea salt and see tangle (doenjang C) showed approximately 89.1% and 70% inhibitory effect on the mutagenesis induced by MNNG and 4NQO against TA100 strain, whereas 84.4% inhibitions were observed on the mutagenesis induced by 4NQO against TA98 strain. The cytotoxic effects of doenjang methanol extracts against the cell lines with human cervical adenocarcinoma (HeLa), human hepatocellular carcinoma (Hep3B), human gastric carcinoma (AGS), human lung carcinoma (A549) and human breast adenocarcinoma (MCF-7) were inhibited with the increase of the extract concentration. The treatment of 1.0 mg/mL doenjang C of methanol extracts showed strong cytotoxicities of 71%, 74.4%, 66.2%, 77.3%, and 71.2% against HeLa, Hep3B, AGS, A549, and MCF-7, respectively. In contrast 1 mg/mL treatment of doenjang C methanol extracts had only $10{\sim}40%$ cytotoxicity on normal human embryonal kidney cell (293). Doenjang methanol extract inhibited significantly the tumor growth in mice injected sarcoma-180 cells. Especially, doenjang C methanol extract showed an inhibition of tumor cell activity of 33% by the administration of 25 mg/kg methanol extracts.
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