• Korean Journal of Environmental Agriculture
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• v.37 no.4
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• pp.235-242
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• 2018

BACKGROUND: About 81% of nitrous oxide ($N_2O$) emissions from agricultural land to the atmosphere is due to nitrogen (N) fertilizer application. Mitigation of $N_2O$ emissions can be more effective in controlling biochemical processes such as nitrification and denitrification in the soil rather than decreasing fertilizer application. The use of urease inhibitors is an effective way to improve N fertilizer efficiency and reduce $N_2O$ emissions. Several compounds act as urease inhibitors, but N-(n-butyl) thiophosphoric triamide (NBPT) has been used worldwide. METHODS AND RESULTS: Hot pepper and chinese cabbage were cultivated in five treatments: standard fertilizer of nitrogen-phosphorus-potassium(N-P-K, $N-P_2O_5-K_2O$: 22.5-11.2-14.9 kg/ha for hot pepper and $N-P_2O_5-K_2O$: 32.0-7.8-19.8 kg/ha for chinese cabbage), no fertilizer, and NBPT-treated fertilizer of 0.5, 1.0, and 2.0 times of nitrogen basal application rate of the standard fertilizer, respectively in Gyeonggi-do Hwaseong-si for 2 years(2015-2016). According to application of NBPT-treated fertilizer in hot pepper and chinese cabbage, $N_2O$ emission decreased by 19-20% compared to that of the standard fertilizer plot. CONCLUSION: NBPT-treated fertilizer proved that $N_2O$ emissions decreased statistically significant in the same growth conditions as the standard fertilization in the hot pepper and chinese cabbage cultivated fields. It means that NBPT-treated fertilizer can be applied for N fertilizer efficiency and $N_2O$ emissions reduction.

• The Korean Journal of Vision Science
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• v.20 no.4
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• pp.543-551
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• 2018

Purpose : The purposes of this study were to evaluate the changes of intraocular pressure according to corneal ablation amount after corneal refractive surgery and the changes of intraocular pressure according to refractive errors before corneal refractive surgery. Methods : The mean age of adults who underwent LASIK corneal refractive surgery were $37.34{\pm}7.42years$, and 108 adults(48 males, 60 females) were participated in this study. Refractive errors, intraocular pressure, and corneal ablation amount were measured using an autorefractor, a noncontact tonometer, and an excimer laser. All test values were considered statistically significant when p<0.05. Results : The mean intraocular pressure before corneal refractive surgery was $15.08{\pm}2.60mmHg$ in males and $14.16{\pm}2.67mmHg$ in females. The decrease of intraocular pressure after corneal refractive surgery were 4.22mmHg in males and 3.61mmHg in females. Spherical equivalent power were $-3.89{\pm}2.17D$ in males and $-4.45{\pm}2.92D$ in females before corneal refractive surgery, and $-0.10{\pm}0.46D$ in males and $-0.04{\pm}0.46D$ in females after corneal refractive surgery. The corneal ablation amount after corneal refractive surgery were statistically significant, with $53.95{\mu}m$ in males and $61.26{\mu}m$ in females. There was significant correlation between corneal ablation amount and decrease of intaocluar pressure(r=0.2299, p<0.001). As the growth of corneal ablation amount in males, the decrease of intraocular pressure was significantly increased. As the growth of refractive error, the amount of decrease in intraocular pressure was also significant. The decrease of intraocualr pressure were $3.04{\pm}2.18mmHg$ in low refractive error, $4.10{\pm}2.16mmHg$ in middle refractive error, and $4.65{\pm}3.29mmHg$ in high refractive error. Conclusion : We found that intraocular pressure decreased after corneal refractive surgery by noncontact tonometer and the change of intraocular pressure which is an important index for glaucoma diagnosis, may affect the judgment of eye disease. We think that a preliminary questionnaire whether corneal refractive surgery is necessary for the measurement of intraocular pressure.

• The Korean Journal of Vision Science
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• v.20 no.4
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• pp.561-568
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• 2018

Purpose : The size and regular array of the collagen fibers in the corneal stroma have very close correlation with transparency. Simulation was carried out to investigate the change of light transmittance according to the array structure and collagen fiber layer thickness. Methods : The collagen fibers in corneal stroma were arranged in regular hexagonal, hexagonal, square and random shapes with OptiFDTD simulation software, and the light transmittance was analyzed. In square array, the light transmittance according to the density change was confirmed by when the number of collagen fibers in the simulation space was the same and the light transmittance was examined when the number and density of collagen fibers were changed. Results : When the number of collagen fibers is the same, the density becomes smaller and the thickness of the fibrous layer becomes thicker in order of arrangement of square, regular hexagonal, random and hexagonal. As a result of measuring the light transmittance by changing the array structure, the light transmittance measured at the detector at the same position was almost similar regardless of the array structure. In the detectors D0, D1, D2 and D3, the maximum transmittance is shown in square, hexagonal and square, regular hexagonal and regular hexagonal array structure, and the minimum transmittance is hexagonal, random, hexagonal and square, and square array structure. However, the difference between the maximum transmittance and the minimum transmittance was almost the same within 1%. When the number of collagen fibers was the same, the light transmittance of the rectangular array structure decreased with increasing fiber layer thickness. And as the thickness increased, the light transmittance decreased more when the number of collagen fibers decreased. Conclusion : Even though the collagen array structure changed, the light transmittance is almost similar regardless of the arrangement structure. However, as the array structure was changed, the thickness of the collagen fiber layer changed, and as the thickness increased, the light transmittance decreased. In other words, the transparency of the corneal stroma is more closely related to the thickness of the fibrous layer than the array of collagen fibers.

• Korean Journal of Environment and Ecology
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• v.33 no.1
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• pp.1-8
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• 2019

Pintail (Anas acuta) is the major wintering bird in South Korea and known as a major mediator of the highly pathogenic avian influenza (HPAI). Pintail migrates long distances between Russian Siberia and Korea. This species prefers a rice paddy area as their winter habitat. The purpose of this study is to provide the data necessary for the conservation and management of bird habitats in Korea by understanding the wintering home-range and habitat of pintail in Korea. We captured six pintails using a cannon-net in the winter of 2015 and attached the GPS-mobile phone based telemetry (WT-300) on them to study the wintering home-range and wintering habitat. We analyzed the tracking location data using ArcGIS 9.0 Animal Movement Extension and calculated Kernel Density Estimation (KDE) and Minimum Convex Polygon (MCP). The average home-range in the wintering ground analyzed by MCP was $677.3km^2$ (SD=130.2, n=6) while the maximum and minimum were $847.7km^2$ and $467.5km^2$, respectively. Extents of home-range analyzed by KDE were $194.7km^2$ (KDE 90%), $77.4km^2$ (KDE 70%), and $35.3km^2$ (KDE 50%). The pintails mostly used both sea and paddy field as habitat in the winter season and utilized paddy fields more during the nighttime and than the daytime. We concluded that the home-range and habitat of pintails in the winter could be used as the reference data for the preservation of species, management of habitats, and coping with a breakout of HPAI.

• Journal of Environmental Impact Assessment
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• v.27 no.6
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• pp.604-617
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• 2018

As the depletion of forests became more widespread due to the increase in the number of roads, the research was conducted on the relationship between temperature and humidity in the forests, assuming that the forests around the roads were affected. Through the forest monitoring, the temperature and humidity of coniferous forests and broadleaf forests in Sedong and Gongju areas were observed at three point of 10m, 20m and 30m from the road boundary to the inside of the forest, respectively. In Yeongdong area, for more reliable results, it was observed from the point of 0m, 10m, and 20m. During the study period, so it was expected the change in tree growth was small, the change of temperature and humidity inside the forest by the road was compared with the temperature and humidity from the road to the inside of the forest from September 2017 to January 2018, the changes of temperature and humidity inside the forest due to linear development such as roads were quantitatively analyzed. Using the HOBO data logger (MX2301, Onset Corp.), the temperature and humidity changes of each site were measured, and the average of the changes have been analyzed monthly. In the case of Gongju coniferous forests in September 2017, the average weekly temperature is $0.57^{\circ}C$ higher than the forest outside from the forest boundary and $1.23^{\circ}C$ higher than the inside of the forest, at night in November 2017, in Sedong broadleaf forests. That is, the ability to control the temperature and humidity of the forests along the road was larger and less variable as the distance from the road boundary to the inside of the forest increased. In this study, it is considered that the high degree of change in temperature and humidity of the forest and the surrounding area due to artificial linear development such as roads will affect the growth of trees. This results could serve as a basis for studying the quantitative scope of linear development affecting forest growth and for managing forest change caused by linear development.

• Journal of Bio-Environment Control
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• v.28 no.3
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• pp.185-196
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• 2019

Abstract. This research was conducted to figure out the optimal size of the plug cell and seedling raising period in 'Nongwoo' and 'Nonghyeop' cultivars. In the first experiment on effect of plug cell size on growth of squash, seedlings were transplanted into hydroponic cultivation beds at different growing stages: Those in 32-cell trays with 3-4 true leaves at 25 days after sowing, those in 50-cell trays with 2 true leaves at 15 days after sowing, those in 105-cell trays just before a true leaf development, and those in 162-cell trays with only cotyledons at 8 days after sowing. In the second experiment on effect of seedling raising period on growth of squash, it was conducted to have different sowing dates. But the same transplanting date, based on the results of Experiment 1, and compared the differences in growth and fruit productivity as affected by plug cell size in the same way with experiment 1 including the cultivars and environmental conditions. After setting the transplanting date in advance, the number of days for sowing were calculated back for each treatment. In the first experiment, plant height was the greatest in 105-cell trays followed by 162, 50 and 32-cell trays in both cultivars. The best fruit quality was found in different treatments depending on the cultivars, although it was the lowest in 32-cell trays in both cultivars. The fruit quality was not significantly different among those from cell sizes. Therefore, when raising seedlings in 105-cell trays, the period of raising seedlings can be shortened as compared with the conventional 32-cell trays, and this change could reduce the workforce required for growing and transplanting seedlings. In the second experiment, after transplanting, shoot height and leaf width in the first measurement in both cultivars were greater in the 32-cell treatment. However, the last measurement after four weeks showed no significant difference in plant height, but significantly greatest leaf width in the smallest cell treatment, even as compared with that in 32-cell treatment. In case of 'Nongwoo', length and weight of the first harvested fruit showed the highest values in the treatment of 105-cell trays. In case of 'Nonghyeop' the 162-cell treatment along with the 105-cell treatment showed greatest length and weight of the first fruits. From these results, zucchini plug seedlings can be raised in plug trays with reduced cell sizes than the conventional 32-cell trays with improved fruit productivity.

• Journal of Life Science
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• v.29 no.9
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• pp.1002-1009
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• 2019

Early life stress (ELS) increases the risk of depression. ELS may be involved in the susceptibility to subsequent stress exposure during adulthood. We investigated whether epigenetic mechanisms of p11 promoter affect the vulnerability to chronic unpredictable stress (CUS) induced by the maternal separation (MS). Mice pups were separated from their dams (3 hr/day from P1-P21). When the pups reached adulthood, we applied CUS (daily for 3 weeks). The levels of hippocampal p11 expression were analyzed by quantitative real-time PCR. The levels of acetylated and methylated histone H3 at p11 promoter were measured by chromatin immunoprecipitation. Depression-like behavior was measured by the forced swimming test (FST). The MS and CUS group exhibited significant decreases in p11 mRNA level and the MS plus CUS group had a greater reduction in this level than the CUS group. The MS plus CUS group also resulted in greater reduction in H3 acetylation than the CUS group. This reduction was associated with an upregulation of histone deacetylase 5. Additionally, the MS plus CUS group showed a greater decrease in H3K4met3 level and a greater increase in H3K27 met3 level than the CUS group. Consistent with the reduction of p11 expression, the MS plus CUS group displayed longer immobility times in the FST compared to the control group. Mice exposed to MS followed by CUS had much greater epigenetic alterations in the hippocampus compared to adult mice that only experienced CUS. ELS can exacerbate the effect of stress exposure during adulthood through histone modification of p11 gene.

• Journal of Life Science
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• v.29 no.8
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• pp.904-915
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• 2019

Prostate cancer (PCa) is one of the most metastatic tumor. Although hormone therapy or surgical castration is mostly conducted to treat PCa, it has a lot of side effects. Recently, many researchers have been exploring the tumor microenvironment to remedy these circumstances. Immune cells, especially macrophages, are an important composition of the tumor microenvironment. Under normal conditions, macrophages exhibit mild tumoricidal activity against tumors. However, once activated by interferon gamma or lipopolysaccharides, macrophages can kill cancer cells directly or indirectly by secreting cytokines and chemokines. In this study, murine macrophage RAW 264.7 cells were treated with Phellinus linteus extract. To analyze their pro-inflammatory phenotype, we were used several assays such as a real-time polymerase chain reaction, an enzyme-linked immunosorbent and nitric oxide assay. Prostate cancer cells were treated with the RAW 264.7-conditioned media, which was identified as a pro-inflammatory nature, for 48 h, and the expression of epithelial-mesenchymal transition (EMT)-related genes was determined. Not only N-cadherin, Snail, Twist, Slug, and Cadherin 11, which are mechenchymal-related proteins, were decrease, but epithelial marker of E-cadherin was increased. In addition, the mRNA level of vimentin, ccl2, and vegfa were decreased, as the EMT is closely related to the migration and invasion of cancer cells. In conclusion, the RAW 264.7-conditioned media stimulated with P. linteus extract inhibited migration and invasion and regulated the EMT pathway in human prostate cancer cells.

• Journal of Life Science
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• v.29 no.8
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• pp.861-870
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• 2019

This study was undertaken to establish optimum medium compositions for cost-effective mannitol production by Leuconostoc mesenteroides SRCM201425 isolated from kimchi. L. mesenteroides SRCM21425 from kimchi was selected for efficient mannitol production based on fructose analysis and identified by its 16S rRNA gene sequence, as well as by carbohydrate fermentation pattern analysis. To enhance mannitol production by L. mesenteroides SRCM201425, the effects of carbon, nitrogen, and mineral sources on mannitol production were first determined using Plackett-Burman design (PBD). The effects of 11 variables on mannitol production were investigated of which three variables, fructose, sucrose, and peptone, were selected. In the second step, each concentration of fructose, sucrose, and peptone was optimized using a central composite design (CCD) and response surface analysis. The predicted concentrations of fructose, sucrose, and peptone were 38.68 g/l, 30 g/l, and 39.67 g/l, respectively. The mathematical response model was reliable, with a coefficient of determination of $R^2=0.9185$. Mannitol production increased 20-fold as compared with the MRS medium, corresponding to a mannitol yield 97.46% when compared to MRS supplemented with 100 g/l of fructose in flask system. Furthermore, the production in the optimized medium was cost-effective. The findings of this study can be expected to be useful in biological production for catalytic hydrogenation causing byproduct and additional production costs.

• The Korean Journal of Archival Studies
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• no.39
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• pp.193-228
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• 2014

In recent years, as well as management of public records, interest in the private archive of large and small is growing. Dedicated archive has various types. In addition, lack of personnel and budget, personnel records management professional because the absence, that help you maintain these records in a systematic manner is not easy. Request to the system have continued to rise, but the budget and professionals in order to solve this problem are missing. As breakthrough of the burden to the system with archive dedicated, it introduces the trends and meaning of public recording system, and was examined in detail AtoM function. AtoM is public land can be made by a method that requires a Web service, the database server. Without restrictions, including the advantage of being available free of charge, by the application or operating system specific, installation and operation is convenient. In addition, compatibility, and is highly scalable, AtoM use and convenient archive of private experiencing a shortage of personnel and budget. Because in terms of data management, and excellent interoperability and search share, and use, it is possible in the future, it favors also documentary use through a network of inter-agency archives and private. In addition, Enhancements exhibition services through cooperation with Omeka, long-term storage through Archivematica, many discussion is needed. Public centered around the private area of the recording management spilling expanded, open-source software allows to balance the recording system will be able to play an important role. In addition, the efforts of academia and in the field, close collaboration between the open source recording system through a user study should be continued. Furthermore, co-operation and sharing of private archives expect come true.