• Journal of Forest and Environmental Science
• /
• v.15 no.1
• /
• pp.107-116
• /
• 1999

For the study on the variation of Pinus densiflora S. et Z. natural populations. 13 stands were selected in Kangwondo region in 1994. They were located in Hyoja-Dong, Chunchon-Si [plot 1]; Sinnam-Myun, lnje-Gun [plot 2]; Buk-Myun, lnje-Gun [plot 3]; Seo-Myun, Yangyang-Gun [plot 4 and 5]; Sonyang-Myun, Yangyang-Gun [plot 6]; Toseong-Myun, Goseong-Gun [plot 7]; Synrim-Myun, Wonju-Si [plot 8]; Dunnae-Myun, Hoengsong-Gun [plot 9]; Jinbu-Myun, Pyungchang-Gun [plot 10]; Sogumgang, Kangneung-Si [plot 11]; Seo-Myun, Ulchin-Gun [plot 12]; and Mitan- Myun, Pyungchang-Gun [plot 13]. The morphological characteristics of needles and cones were investigated for twenty individual trees from each population. The results are summarized as follows: 1. Among needle characteristics, length, the number of serration. and the number of stoma row were significantly different among populations and between individuals within a population. They were 69.3~91.9mm, 25.1~28.7 per 0.5cm, and 4.1~6.2 rows for abaxial side and 2.9~4.6 rows for adaxial side, respectively. 2. Among cone characters surveyed. length and diameter were significantly different among populations and between individuals within a population. They were 31.1~43.7mm and 20.0~24.1mm, respectively. 3. A highly positive correlation was observed between the number of stoma row of abaxial side and that of adaxial side. and between length and diameter of cone. But the number of serration was negatively correlated with all traits. 4. The distance from seashore was positively correlated with needle length and the number of serration, but negatively correlated with the number of stoma row and cone length. However, the altitude was positively correlated with the number of serration, but negatively correlated with the number of stoma row and cone length. 5. Cluster analysis using average linkage method for needle and cone characteristics showed two groups to Euclidean distance 1.0. They were group I consisting of plots 1, 8, 12, and 13 and group II of plots 2, 3, 4, 5, 6, 7, 9, 10, and 11. However, group II was divided again to Euclidean distance 0.8, that is a group including plots 2, 3, 9, and 11 and the other group comprising plots 4, 5, 6, 7, and 10.

• Journal of dental hygiene science
• /
• v.13 no.1
• /
• pp.36-44
• /
• 2013

The purpose of this study was to provide basic data for healthy and effective dental management by providing the knowledge and information on periodontal health promotion as well as high dental health interests, by doing a comparative analysis of the effect of dental health knowledge and oral hygiene management skills of people in their 20s on periodontal health status assessment, to find periodontal disease early and prevent it targeting 130 adults in their 20s who visited dental hygienic lab for comprehensive dental hygiene care and treatment from September 25 to October 30. The data collected used SPSS 18.0. To present the technical characteristics of the data, frequency was used and chi-squared test through cross-analysis was conducted to investigate dental health knowledge and the relevance between the variables of oral hygiene management skills of general characteristics. To examine periodontal health status assessment, t-test and One-way ANOVA and Turkey post-hoc tests were carried out at the 5% significance level. Regression analysis was performed to investigate the impact of dental health knowledge and oral hygiene management skills on dental health status assessment. As a result of this study, as dental health knowledge on tooth brushing of people in their 20s increased, plaque index reduced, as dental health knowledge on periodontal disease increased, plaque index reduced. Dental health status assessment according to a total number of times tooth brushing, tooth brushing methods, whether to use dental hygiene devices, smoking status, drinking frequency per week, whether to have oral health education was statistically significant. To improve the level of dental health knowledge and oral hygiene management skills, oral health education should be strengthened as well as the publicity through the media to have the information on oral health and learn it.

• Journal of dental hygiene science
• /
• v.17 no.4
• /
• pp.283-289
• /
• 2017

The water discharged from dental unit waterlines (DUWLs) is heavily contaminated with bacteria. The development of efficient disinfectants is required to maintain good quality DUWL water. The purpose of this study was to establish a DUWL biofilm model using well-plates to confirm the effectiveness of disinfectants in the laboratory. Bacteria were obtained from the water discharged from DUWLs and incubated in R2A liquid medium for 10 days. The bacterial solution cultured for 10 days was made into stock and these stocks were incubated in R2A broth and batch mode for 5 days. Batch-cultured bacterial culture solution and polyurethane tubing sections were incubated in 12-well plates for 4 days. Biofilm accumulation was confirmed through plating on R2A solid medium. In addition, the thickness of the biofilm and the shape and distribution of the constituent bacteria were confirmed using confocal laser microscopy and scanning electron microscopy. The average accumulation of the cultured biofilm over 4 days amounted to $1.15{\times}10^7CFU/cm^2$. The biofilm was widely distributed on the inner surface of the polyurethane tubing and consisted of cocci, short-length rods and medium-length rods. The biofilm thickness ranged from $2{\mu}m$ to $7{\mu}m$. The DUWL biofilm model produced in this study can be used to develop disinfectants and study DUWL biofilm-forming bacteria.

• Journal of Korean Society of Archives and Records Management
• /
• v.1 no.1
• /
• pp.37-52
• /
• 2001

1. establishment and development of China archives science: With the centuries-old history of archives and archives management, early China archives science came into being in 1930s, and the research pushed forward by archives enterprise has made great achievements since then. 1.1 Expanding research fields: Foundation

• Korean Journal of Food Science and Technology
• /
• v.45 no.6
• /
• pp.714-720
• /
• 2013

We investigated the brewing and fermenting characteristics of makgeolli produced from high-yielding rice varieties. We used the high-yielding indica rice varieties, Anda and Dasan 1, and the japonica varieties, Deuraechan and Boramchan. Our results showed that the rice protein level was the highest in makgeolli produced from Anda ($7.5{\pm}0.2%$), while the amylose level was the lowest in makgeolli produced from the mid- to late-maturing japonica varieties (Deuraechan, $18.9{\pm}0.7%$; Boramchan, $18.9{\pm}1.4%$). Samples of makgeolli produced from Anda, Deuraechan, and Boramchan by using the Ipguk (Koji) method had an alcohol content of 16.6-17.4% on completion of fermentation. By contrast, makgeolli produced from Dasan 1 had an alcohol content of 14.3%; further, the makgeolli differed significantly in the sensory test and was scored as excellent regarding comprehensive preference. For makgeolli produced by using the uncooked rice fermentation method, samples produced from the indica varieties, Anda and Dasan 1, had lower alcohol contents (17.2% and 17.0%, respectively) and higher total acidity levels (0.33% and 0.31%, respectively) than did samples produced from the japonica varieties, Deuraechan and Boramchan (0.28% for both). In the sensory test, samples produced from Anda and Dasan 1 performed significantly better regarding scent, swallowing, and comprehensive preference.

• Korean Journal of Food Science and Technology
• /
• v.45 no.2
• /
• pp.148-155
• /
• 2013

Sulfoxaflor is a new active ingredient within the sulfoximine insecticide class that acts via a unique interaction with the nicotinic receptor. The MRLs (maximun residue limit) of sulfoxaflor in apple and pear are set at 0.4 mg/kg and that in pepper is set at 0.5 mg/kg. The purpose of this study was to develop an analytical method for the determination of sulfoxaflor residues in agricultural commodities using HPLC-UVD and LC-MS. The analysis of sulfoxaflor was performed by reverse phase-HPLC using an UV detector. Acetone and methanol were used for the extraction and aminopropyl ($NH_2$) cartridge was used for the clean-up in the samples. Recovery experiments were conducted on 7 representative agricultural products to validate the analytical method. The recoveries of the proposed method ranged from 82.8% to 108.2% and relative standard deviations were less than 10%. Finally, LC-MS with selected ion monitoring was also applied to confirm the suspected residues of sulfoxaflor in agricultural commodities.

• Korean Journal of Food Science and Technology
• /
• v.45 no.2
• /
• pp.221-226
• /
• 2013

This study aimed to examine the antioxidant, tyrosinase inhibitory, and anti-proliferative activities (A549, G361, HT-29, and MDA-MB-231) of fermented gochujang (made from sword bean cheonggukjang powder (SBC) for 90 days. Gochujang was prepared by adding 0 (SBC 0), 2 (SBC 2), 5 (SBC 5), 8 (SBC 8) and 10% (SBC 10) levels with SBC, and all experiments were measured at diluted levels of 20, 50 and 100 times. The antioxidant activity and tyrosinase inhibitory effect demonstrated that SBC 10 increased approximately 1.2 and 1.1 times compared with SBC 0, respectively, at diluted levels of 50 and 100 times. The anti-proliferative effects of A549, G361, and HT-29 presented that SBC 10 were 2.8, 1.1, and 8.9 times higher compared with SBC 0, respectively, at diluted levels of 50, 20, and 100 times. In the case of MDA-MB-231, SBC 10 was 3.7 times higher compared with SBC 5 at diluted level of 20 times. As a result, we confirmed that SBC gochujang was improved for physiological activities and anti-proliferative effects.

• Korean Journal of Food Science and Technology
• /
• v.49 no.3
• /
• pp.258-264
• /
• 2017

The stability and accuracy of ultra-performance liquid chromatography (UPLC) used for evaluating the contents of rutin and quercetin in tartary buckwheat (Fagopyrum tataricum Gaertn.) seeds extracted by seven different extraction methods were determined. The seven extraction methods were reflux extraction (RE), ultra-sonification extraction (UE), stirrer extraction (SE), RE after UE (UE+RE), RE after SE (SE+RE), UE after SE (SE+UE), and RE with UE after SE (SE+UE+RE). Among the seven extraction methods used, RE yielded comparatively higher contents of rutin (2,277 mg/ 100 g) and quercetin (158 mg/100 g) than those yielded by other six extraction methods. The intra-day repeatability and inter-day precision of RE was 0.4-3.2% considering relative standard deviation (RSD), while accuracy was 88.8-102.4%. Therefore, RE with UPLC would be a rapid, accurate, and stable method for analyzing rutin and quercetin contents in tartary buckwheat.

• Horticultural Science & Technology
• /
• v.33 no.4
• /
• pp.605-617
• /
• 2015

The ultimate goal of this research is to develop a botanical biofiltration system that combines a green interior, biofiltering, and automatic irrigation to purify indoor air pollutants according to indoor space and the size of biofilter. This study was performed to compare the stability of air flow characteristics and removal efficiency (RE) of fine dust within a wall-typed (vertical) botanical biofilter depending on humidifying cycle and to investigate RE of volatile organic compounds (VOCs) by the biofilter. The biofilter used in this experiment was designed as an integral form of water metering pump, water tank, blower, humidifier, and multi-level planting space in order to be suitable for indoor space utilization. As a result, relative humidity, air temperature, and soil moisture content (SMC) within the biofilter showed stable values regardless of three different humidifying cycles operated by the metering pump. In particular, SMCs were consistently maintained in the range of 27.1-29.7% during all humidifying cycles; moreover, a humidifying cycle of operating for 15 min and pausing for 45 min showed the best horizontal linear regression (y = 0.0008x + 29.09) on SMC ($29.0{\pm}0.2%$) during 120 hour. REs for number of fine dust (PM10) and ultra-fine dust (PM2.5) particles passed through the biofilter were in the range of 82.7-89.7% and 65.4-73.0%, respectively. RE for weight of PM10 passed through the biofilter was in the range of 58.1-78.9%, depending on humidifying cycle. REs of xylene, ethyl benzene, total VOCs (TVOCs), and toluene passed through the biofilter were in the range of 71.3-75.5%, while REs of benzene and formaldehyde (HCHO) passed through the biofilter were 39.7% and 44.9%, respectively. Hence, it was confirmed that the wall-typed botanical biofilter suitable for indoor plants was very effective for indoor air purification.

• Horticultural Science & Technology
• /
• v.30 no.5
• /
• pp.557-567
• /
• 2012

This study was conducted to achieve basal information for the development of tomato cultivars with disease resistances through marker-assisted backcross (MAB). Ten inbred lines with TYLCV, late blight, bacterial wilt, or powdery mildew resistance and four adapted inbred lines with superior horticultural traits were collected, which can be useful as the donor parents and recurrent parents in MAB, respectively. Inbred lines collected were evaluated by molecular markers and bioassay for confirming their disease resistances. To develop DNA markers for selecting recurrent parent genome (background selection) in MAB, a total of 108 simple sequence repeat (SSR) primer sets (nine per chromosome at average) were selected from the tomato reference genetic maps posted on SOL Genomics Network. Genetic similarity and relationships among the inbred lines were assessed using a total of 303 polymorphic SSR markers. Similarity coefficient ranged from 0.33 to 0.80; the highest similarity coefficient (0.80) was found between bacterial wilt-resistant donor lines '10BA333' and '10BA424', and the lowest (0.33) between a late blight resistant-wild species L3708 (S. pimpinelliforium L.) and '10BA424'. UPGMA analysis grouped the inbred lines into three clusters based on the similarity coefficient 0.58. Most of the donor lines of the same resistance were closely related, indicating the possibility that these lines were developed using a common resistance source. Parent combinations (donor parent ${\times}$ recurrent parent) showing appropriate levels of genetic distance and SSR marker polymorphism for MAB were selected based on the dendrogram. These combinations included 'TYR1' ${\times}$ 'RPL1' for TYLCV, '10BA333' or '10BA424' ${\times}$ 'RPL2' for bacterial wilt, and 'KNU12' ${\times}$ 'AV107-4' or 'RPL2' for powdery mildew. For late blight, the wild species resistant line 'L3708' was distantly related to all recurrent parental lines, and a suitable parent combination for MAB was 'L3708' ${\times}$ 'AV107-4', which showed a similarity coefficient of 0.41 and 45 polymorphic SSR markers.