• Applied Microscopy
• /
• v.37 no.2
• /
• pp.93-102
• /
• 2007

Secretory leukocyte protease inhibitor (SLPI) was known as one of bacterial lipopolysaccharide (LPS)-induced products of macrophage. Macrophages play an important role in the development of inflammatory responses by secreting an array of cytokines and chemokines in a tissue microenvironment. To identify the function and relationship between potent growth factors and SLPI after LPS stimulation, we conducted reverse transcriptase polymerase chain reaction (RT-PCR) and Western blots for the detection of mRNA and protein expression of SLPI and growth factors such as VEGF, PDGF, bFGF after 100 ng LPS stimulation on the RAW264.7 cells. The result of RT-PCR was showed SLPI mRNA expression was increased from 60 min to 48h in RAW 264.7 cells after incubation with LPS. VEGF and PDGF mRNA was expressed highly at initial stage by LPS stimulation. The mRNA of bFGF and type I collagen was very weakly expressed after LPS stimulation. SLPI protein level was increased likely the mRNA levels in RAW 267.7 cells. Additionally, phase contrast and scanning electron microscopic observation demonstrated that the LPS induce the change of morphology of the RAW264.7 cells. From these results, it suggest that expression of SLPI by LPS treatment may associate with VEGF and PDGF expression in RAW264.7 cells.

• Applied Microscopy
• /
• v.35 no.1
• /
• pp.41-56
• /
• 2005

Present study was performed to observe the tegumental ultrastructures by the developmental stages which derived from the experimental life cycle of Spirometra erinacei in laboratory conditions. In SEM view, coracidium was spherical in shape with numerous cilia, and its surface was covered with long cilia, tuberclelike projections with millet-like processes, and small holes. The body surface of procercoid was covered with numerous pointed microtriches except that of frontal pit with stout spine-like ones. However that of cercomer was covered with somewhat sparse blunt-tiped microtriches. Plerocercoids of 3 days old resembled the mature procercoid in shape, and their frontal pits were covered with numerous stout spine-like microtriches. However frontal pit and body surface in more than 5 days old ones were covered with conoid microtriches. On the surface of adult scolex, hairly long filamentous and stout short microtriches were mixedly distributed. Filamentous microtriches were more densely distributed in the anterior portion than in the posterior of scolex. The neck and immature proglottid were covered with only stout short conoid microtriches. In TEM view of coracidia, embryophore and oncosphere were obviously distinguished. The embryophore contained numerous glycogen particles, mitochondria and lipid granules. The cilia on the surface of embryophore rooted in the coracidial sheath, and consisted of 9 pairs of microtubules and 2 core complex. The oncosphere was covered with a thin and unarmed tegument, and was multi-nucleated. The protoplasmic layer of procercoid and plerocercoid consisted of disc-shaped bodies, vacuoles and mitochondria. Their tegumental cells commonly retained a nucleus, granular endoplasmic reticulums and secretory granules. The protoplasmic layer of plerocercoid was more compacted than that of procercoid. From the above results, it was confirmed that the tegumental ultrastructures are something different according to the developmental stages of S. erinacei.

• Applied Microscopy
• /
• v.42 no.2
• /
• pp.61-66
• /
• 2012

Ultrastructural characteristics of blacktip grouper, Epinephelus fasciatus spermatozoa were investigated using transmission and scanning electron microscopy. The spermatozoa of E. fasciatus consisted of a spherical head part, a midpiece with cytoplasmic canal entrance and a flagellum with lateral fins. Internal ultrastructurally, the nucleus contains high electron dense chromatin having granular particles and has no acrosome. The centriolar complex lies outside of the nuclear fossa and it is connected by the osmophilic filaments. Also the osmophilic filaments connect between the centriolar complex and the nuclear membrane. The midpiece contains eight to nine spherical mitochondria, cytoplasmic canal and necklaces. The flagellum has a typical 9+2 axonemal structure. The lateral fins contain vesicles and a typical 9+2 axonemal structure. Consequently this study contributes to comparative grouper spermatology and provide useful systematic taxonomic characters.

• 한국유가공학회:학술대회논문집
• /
• 2006.06a
• /
• pp.29-45
• /
• 2006

최근에 들어 웰빙(well-being)과 함께 로하스(LOHAS: Lifestyle of Health and Sustainability)란 개념이 급속도로 확산되고 있으며, 이를 만족하는 식품으로 친환경 농산물과 유기 가공 식품이 대두되고 있다. 여러 마케팅 조사에서도 친환경 농산물과 유기 가공 식품의 소비자 선호도는 상당히 높게 나타난다. 이러한 다양한 소비자의 요구에 맞춰 우리의 2세에게 더욱 안전하고 좋은 품질의 이유식을 제공하고 나아가 한계에 이른 '지구와 환경을 해치지 않는 지속가능 한 소비'를 유도하고자 업체들은 유기농 이유식을 개발하고 있다. 유기농 소비자 10명 가운데 7명은 최근의 웰빙 붐 때문에 유기농에 관심을 가지게 됐으며, 가격이 다소 비싸더라도 가족의 건강을 위해 친환경 농산물을 구입하는 것으로 나타났으며, 국내외를 막론하고 친환경 농산물과 유피 가공 식품의 시장 동향을 살펴보면 지속적으로 증가하고 있으나, 아직까지 일반 제품에 비해 미미한 수준이며, 국내는 인증 제도가 없으나, 유기 가공 식품표시기준에 따라 표시되고 있다. 일반적으로 이유식이라 함은 식품공전에서 영 ${\cdot}$ 유아용 곡류 조제식만을 말하지만 넓게 축산물의 조제유류, 영아용 조제식, 성장기용 조제식, 기타 영 ${\cdot}$ 유아식을 포함할 수 있으며, 유기농 이유식 제조 현황을 살펴보면 동결, 분무, 드럼 등의 건조와 퍼핑이나 Extruder를 통한 알파화로 원료를 가공하여 은 살균, 멸균, 균질, 혼합, 건조, 냉각, 포장 등의 다양한 공정으로 이루어진 분무건조나 유동층이나 연속식 과립기를 사용하여 분말이나 입자형 이유식을 생산하며, 동결 건조를 하여 열수를 가해 죽 상태로 먹을 수 있도록 한 제품, 데워서 떠 먹일 수 있도록 병조림이나 레토르트 유동식 제품, 바로 마실 수 있는 쥬스류, 두유류, 과자 등으로 생산하고 있다. 주된 메이저4사는 일동후디스, 남양유업, 매일유업, 파스퇴르유업으로 다양한 제품들을 생산하고 있으며, 두유를 위주로 한 정식품, 연세우유와 종근당건강, 다양한 다국적 기업들의 제품이 수입되고 있는 실정이다. 이유식 시장도 96년 하반기부터 정체를 보이는 상태다. 2002년도 이유식 시장은 약 1,300억원 규모로 업체들의 고가 신제품과 유기농 이유식 출시로 판매 금액은 소폭 상승하였으나 판매 수량은 큰 변동이 없다. 저출산에 따른 양육비 집중과 유기농 제품에 대한 저변이 크게 확산돼 베이비 웰빙은 어느 정도 예견된 일이거니와 이미 미국 등 선진국에선 유아에 그치지 않고 아동까지 유기농과 고급 원료를 사용한 고가의 웰빙 제품이 봇물을 이루고 있는 상황이어서 유아식 시장은 어느 때보다 경쟁이 치열할 것이며, 국내 식품 업체들이 너도나도 유기농 제품과 매장사업에 뛰어들고 있다. 유기 가공 식품의 지속적인 발전을 위해서 (1) 국내산/수입산 공통으로 인증 제도가 도입되고 원료 농산물뿐만 아니라, 모든 공정이나 공장에 대한 종합적 관리와 동일한 인증 표시가 이루어져야 한다. (2) 정부 부처의 이기를 타파할 수 있는 법정부적 기구나 대책이 필요하다. (3) 유기 가공 식품을 취급하는 업계에서는 기존 3%의 비의도적 혼입치의 고려나 상한선 재설정을 요구하고 진정한 유기 가공 식품으로 공정 경쟁한다. (4) 활발한 인증심사원의 교육과 배출로 인증이나 심사기관의 민간 이양을 적극 장려하고 국가관 상호 신뢰를 구축한다. (5) 각 소비자 단체나 소비자는 환경을 살리고 생명체의 공생이라는 관점에서 최선의 길이 유기농 제품임을 인식하고 소비하여 우리의 다음 세대에게서 빌려온 소중한 환경을 물려준다.

• Clean Technology
• /
• v.28 no.3
• /
• pp.203-209
• /
• 2022

An environmental evaluation was conducted by employing LCA methodology for a mechanical seal manufacturing process that uses recycled silicon recovered from end-of-cycle PV modules. The recycled silicon was purified and reacted with carbon to synthesize β-SiC particles. Then the particles underwent compression molding, calcination and heat treatment to produce a product. Field data were collected and the potential environmental impacts of each stage were calculated using the LCI DB of the Ministry of Environment. The assessment was based on 6 categories, which were abiotic resource depletion, acidification, eutrophication, global warming, ozone depletion and photochemical oxidant creation. The environmental impacts by category were 45 kg CO₂ for global warming and 2.23 kg C₂H₄ for photochemical oxide creation, and the overall environmental impact by photochemical oxide creation, resource depletion and global warming had a high contribution of 98.7% based on weighted analysis. The wet process of fine grinding and mixing the raw silicon and carbon, and SiC granulation were major factors that caused the environmental impacts. These impacts need to be reduced by converting to a dry process and using a system to recover and reuse the solvent emitted to the atmosphere. It was analyzed that the environmental impacts of resource depletion and global warming decreased by 53.9% and 60.7%, respectively, by recycling silicon from end-of-cycle PV modules. Weighted analysis showed that the overall environmental impact decreased by 27%, and the LCA analysis confirmed that recycling waste modules could be a major means of resource saving and realizing carbon neutrality.