• Microbiology and Biotechnology Letters
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• v.41 no.1
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• pp.17-25
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• 2013

The thermotolerant methylotrophic yeast Hansenula polymorpha is an attractive model organism for various fundamental studies, such as the genetic control of enzymes involved in methanol metabolism, peroxisome biogenesis, nitrate assimilation, and resistance to heavy metals and oxidative stresses. In addition, H. polymorpha has been highlighted as a promising recombinant protein expression host, especially due to the availability of strong and tightly regulatable promoters. In this study, we investigated the possibility of employing human serum albumin (HSA) as the fusion tag for the secretory expression of heterologous proteins in H. polymorpha. A set of four expression cassettes, which contained the methanol oxidase (MOX) promoter, translational HSA fusion tag, and the terminator of MOX, were constructed. The expression cassettes were also designed to contain sequences for accessory elements including His8-tag, $2{\times}(Gly_4Ser_1)$ linkers, tobacco etch virus protease recognition sites (Tev), multi-cloning sites, and strep-tags. To determine the effects of the size of the HSA fusion tag on the secretory expression of the target protein, each cassette contained the HSA gene fragment truncated at a specific position based on its domain structure. By using the Green fluorescence protein gene as the reporter, the properties of each expression cassette were compared in various conditions. Our results suggest that the translational HSA fusion tag is an efficient tool for the secretory expression of recombinant proteins in H. polymorpha.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.29 no.2
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• pp.109-120
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• 2017

A discounted cost model for preventive maintenance of armor units of rubble-mound breakwaters is mathematically derived by combining the deterioration model based on a discrete-time stochastic process of shock occurrence with the cost model of renewal process together. The discounted cost model of condition-based maintenance proposed in this paper can take into account the nonlinearity of cumulative damage process as well as the discounting effect of cost. By comparing the present results with the previous other results, the verification is carried out satisfactorily. In addition, it is known from the sensitivity analysis on variables related to the model that the more often preventive maintenance should be implemented, the more crucial the level of importance of system is. However, the tendency is shown in reverse as the interest rate is increased. Meanwhile, the present model has been applied to the armor units of rubble-mound breakwaters. The parameters of damage intensity function have been estimated through the time-dependent prediction of the expected cumulative damage level obtained from the sample path method. In particular, it is confirmed that the shock occurrences can be considered to be a discrete-time stochastic process by investigating the effects of uncertainty of the shock occurrences on the expected cumulative damage level with homogeneous Poisson process and doubly stochastic Poisson process that are the continuous-time stochastic processes. It can be also seen that the stochastic process of cumulative damage would depend directly on the design conditions, thus the preventive maintenance would be varied due to those. Finally, the optimal periods and scale for the preventive maintenance of armor units of rubble-mound breakwaters can be quantitatively determined with the failure limits, the levels of importance of structure, and the interest rates.

• Korean Journal of Remote Sensing
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• v.23 no.2
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• pp.145-152
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• 2007

The polarimetric backscattering coefficients of a wet-land rice field which is an experimental plot belong to National Institute of Agricultural Science and Technology in Suwon are measured using ground-based polarimetric scatterometers at 1.8 and 5.3 GHz throughout a growth year from transplanting period to harvest period (May to October in 2006). The polarimetric scatterometers consist of a vector network analyzer with time-gating function and polarimetric antenna set, and are well calibrated to get VV-, HV-, VH-, HH-polarized backscattering coefficients from the measurements, based on single target calibration technique using a trihedral corner reflector. The polarimetric backscattering coefficients are measured at $30^{\circ},\;40^{\circ},\;50^{\circ}\;and\;60^{\circ}$ with 30 independent samples for each incidence angle at each frequency. In the measurement periods the ground truth data including fresh and dry biomass, plant height, stem density, leaf area, specific leaf area, and moisture contents are also collected for each measurement. The temporal variations of the measured backscattering coefficients as well as the measured plant height, LAI (leaf area index) and biomass are analyzed. Then, the measured polarimetric backscattering coefficients are compared with the rice growth parameters. The measured plant height increases monotonically while the measured LAI increases only till the ripening period and decreases after the ripening period. The measured backscattering coefficientsare fitted with polynomial expressions as functions of growth age, plant LAI and plant height for each polarization, frequency, and incidence angle. As the incidence angle is bigger, correlations of L band signature to the rice growth was higher than that of C band signatures. It is found that the HH-polarized backscattering coefficients are more sensitive than the VV-polarized backscattering coefficients to growth age and other input parameters. It is necessary to divide the data according to the growth period which shows the qualitative changes of growth such as panicale initiation, flowering or heading to derive functions to estimate rice growth.

• Journal of Korea Water Resources Association
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• v.52 no.11
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• pp.917-929
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• 2019

Denitrification in streams is of great importance because it is essential for amelioration of water quality and accurate estimation of $N_2O$ budgets. Denitrification is a major biological source or sink of $N_2O$, an important greenhouse gas, which is a multi-step respiratory process that converts nitrate ($NO_3{^-}$) to gaseous forms of nitrogen ($N_2$ or $N_2O$). In aquatic ecosystems, the complex interactions of water flooding condition, substrate supply, hydrodynamic and biogeochemical properties modulate the extent of multi-step reactions required for $N_2O$ flux. Although water flow in streambed and residence time affect reaction output, effects of a complex interaction of hydrodynamic, geomorphology and biogeochemical controls on the magnitude of denitrification in streams are still illusive. In this work, we built a two-dimensional water flow channel and measured $N_2O$ flux from channel sediment with different bed geomorphology by using static closed chambers. Two independent experiments were conducted with identical flume and geomorphology but sediment with differences in dissolved organic carbon (DOC). The experiment flume was a circulation channel through which the effluent flows back, and the size of it was $37m{\times}1.2m{\times}1m$. Five days before the experiment began, urea fertilizer (46% N) was added to sediment with the rate of $0.5kg\;N/m^2$. A sand dune (1 m length and 0.15 m height) was made at the middle of channel to simulate variations in microtopography. In high- DOC experiment, $N_2O$ flux increases in the direction of flow, while the highest flux ($14.6{\pm}8.40{\mu}g\;N_2O-N/m^2\;hr$) was measured in the slope on the back side of the sand dune. followed by decreases afterward. In contrast, low DOC sediment did not show the geomorphological variations. We found that even though topographic variation influenced $N_2O$ flux and chemical properties, this effect is highly constrained by carbon availability.

• Journal of Korea Water Resources Association
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• v.54 no.12
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• pp.1339-1348
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• 2021

In accordance with the River Law, the basic river maintenance plan is established every 5-10 years with a considerable national budget for domestic rivers, and various river surveys such as the river section required for HEC-RAS simulation for flood level calculation are being conducted. However, river survey data are provided only in the form of a pdf report to the River Management Geographic Information System (RIMGIS), and the original data are distributedly owned by designers who performed the river maintenance plan in CAD format. It is a situation that the usability for other purposes is considerably lowered. In addition, when using surveyed CAD-type cross-sectional data for HEC-RAS, tools such as 'Dream' are used, but the reality is that time and cost are almost as close as manual work. In this study, RAUT (River Information Auto Upload Tool), a tool that can solve these problems, was developed. First, the RAUT tool attempted to automate the complicated steps of manually inputting CAD survey data and simulating the input data of the HEC-RAS one-dimensional model used in establishing the basic river plan in practice. Second, it is possible to directly read CAD survey data, which is river spatial information, and automatically upload it to the river spatial information DB based on the standard data model (ArcRiver), enabling the management of river survey data in the river maintenance plan at the national level. In other words, if RIMGIS uses a tool such as RAUT, it will be able to systematically manage national river survey data such as river section. The developed RAUT reads the river spatial information CAD data of the river maintenance master plan targeting the Jeju-do agar basin, builds it into a mySQL-based spatial DB, and automatically generates topographic data for HEC-RAS one-dimensional simulation from the built DB. A pilot process was implemented.

• Clean Technology
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• v.27 no.4
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• pp.350-358
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• 2021

The results of this study shows that the combustor temperature ranged from 848.27 to 1,026.80 ℃, averaging about 976.61 ℃, and the NOx concentration increased as the temperature increased. The urea usage ranged from 291.00 to 693.00 kg d^-1, averaging about 542.34 kg d^-1, and the NOx concentration decreased as the urea usage increased. Residence time was about 3.38 to 9.17 s, averaging about 5.22 s, about 2.61 times larger than the 2 s of the design details. This is 1,086 kg h^-1, averaging about 55.71%, compared to the 1,950 kg h^-1 SRF input permission standard. The combustion chamber area is constant, but the residence time is shown to increase with the decrease of exhaust gas. The O₂/CO ratio was 847.05 to 14,877.34, averaging about 3,111.30, and the NOx concentration slightly increased as the O₂/CO ratio increased. As the combustor temperature and O₂/CO ratio increased, the combustion reaction with nitrogen in the air increased and the NOx concentration slightly increased. As the urea usage and residence time increased, the NOx concentration decreased slightly with an increase in reactivity with NOx. The NOx concentration at the stack ranged from 7.88 to 34.02 ppm with an average of 19.92 ppm, and was discharged within the 60 ppm emission limit value. The NOhx emission factor was 1.058 to 1.795 kg ton^-1, averaging about 1.450 kg ton^-1. This value was about 24.87% of the maximum emission factor of 5.830 kg ton^-1 of other solid fuels. Other synthetic resins and industrial wastes were 79.80% and 43.65% compared to 1.817 kg ton^-1 and 3.322 kg ton^-1, respectively. This value was similar to 1.400 kg ton^-1 of RDF in the NIER notice (2005-9), 10.98% compared to the maximum SRF of 13.210 kg ton^-1. Therefore, the NOx emission factor had a large deviation.

• Journal of the Korean Association of Geographic Information Studies
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• v.21 no.4
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• pp.1-13
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• 2018

Both Platform as a Service (PaaS) as one of the cloud computing service models and the e-government (e-Gov) standard framework from the Ministry of the Interior and Safety (MOIS) provide developers with practical computing environments to build their applications in every web-based services. Web application developers in the geo-spatial information field can utilize and deploy many middleware software or common functions provided by either the cloud-based service or the e-Gov standard framework. However, there are few studies for their applicability and performance in the field of actual geo-spatial information application yet. Therefore, the motivation of this study was to investigate the relevance of these technologies or platform. The applicability of these computing environments and the performance evaluation were performed after a test application deployment of the spatial image processing case service using Web Processing Service (WPS) 2.0 on the e-Gov standard framework. This system was a test service supported by a cloud environment of Cloud Foundry, one of open source PaaS cloud platforms. Using these components, the performance of the test system in two cases of 300 and 500 threads was assessed through a comparison test with two kinds of service: a service case for only the PaaS and that on the e-Gov on the PaaS. The performance measurements were based on the recording of response time with respect to users' requests during 3,600 seconds. According to the experimental results, all the test cases of the e-Gov on PaaS considered showed a greater performance. It is expected that the e-Gov standard framework on the PaaS cloud would be important factors to build the web-based spatial information service, especially in public sectors.

• Journal of Intelligence and Information Systems
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• v.24 no.4
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• pp.33-49
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• 2018

The impact of TV Drama success on TV Rating and the channel promotion effectiveness is very high. The cultural and business impact has been also demonstrated through the Korean Wave. Therefore, the early prediction of the blockbuster success of TV Drama is very important from the strategic perspective of the media industry. Previous studies have tried to predict the audience ratings and success of drama based on various methods. However, most of the studies have made simple predictions using intuitive methods such as the main actor and time zone. These studies have limitations in predicting. In this study, we propose a model for predicting the popularity of drama by analyzing the customer's viewing pattern based on various theories. This is not only a theoretical contribution but also has a contribution from the practical point of view that can be used in actual broadcasting companies. In this study, we collected data of 280 TV mini-series dramas, broadcasted over the terrestrial channels for 10 years from 2003 to 2012. From the data, we selected the most highly ranked and the least highly ranked 45 TV drama and analyzed the viewing patterns of them by 11-step. The various assumptions and conditions for modeling are based on existing studies, or by the opinions of actual broadcasters and by data mining techniques. Then, we developed a prediction model by measuring the viewing-time distance (difference) using Euclidean and Correlation method, which is termed in our study similarity (the sum of distance). Through the similarity measure, we predicted the success of dramas from the viewer's initial viewing-time pattern distribution using 1~5 episodes. In order to confirm that the model is shaken according to the measurement method, various distance measurement methods were applied and the model was checked for its dryness. And when the model was established, we could make a more predictive model using a grid search. Furthermore, we classified the viewers who had watched TV drama more than 70% of the total airtime as the "passionate viewer" when a new drama is broadcasted. Then we compared the drama's passionate viewer percentage the most highly ranked and the least highly ranked dramas. So that we can determine the possibility of blockbuster TV mini-series. We find that the initial viewing-time pattern is the key factor for the prediction of blockbuster dramas. From our model, block-buster dramas were correctly classified with the 75.47% accuracy with the initial viewing-time pattern analysis. This paper shows high prediction rate while suggesting audience rating method different from existing ones. Currently, broadcasters rely heavily on some famous actors called so-called star systems, so they are in more severe competition than ever due to rising production costs of broadcasting programs, long-term recession, aggressive investment in comprehensive programming channels and large corporations. Everyone is in a financially difficult situation. The basic revenue model of these broadcasters is advertising, and the execution of advertising is based on audience rating as a basic index. In the drama, there is uncertainty in the drama market that it is difficult to forecast the demand due to the nature of the commodity, while the drama market has a high financial contribution in the success of various contents of the broadcasting company. Therefore, to minimize the risk of failure. Thus, by analyzing the distribution of the first-time viewing time, it can be a practical help to establish a response strategy (organization/ marketing/story change, etc.) of the related company. Also, in this paper, we found that the behavior of the audience is crucial to the success of the program. In this paper, we define TV viewing as a measure of how enthusiastically watching TV is watched. We can predict the success of the program successfully by calculating the loyalty of the customer with the hot blood. This way of calculating loyalty can also be used to calculate loyalty to various platforms. It can also be used for marketing programs such as highlights, script previews, making movies, characters, games, and other marketing projects.

• Journal of the Korea institute for structural maintenance and inspection
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• v.23 no.3
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• pp.83-91
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• 2019

The purpose of this study is to assess a fire-damaged concrete structure using a digital camera and image processing software. To simulate it, mortar and paste samples of W/C=0.5(general strength) and 0.3(high strength) were put into an electric furnace and simulated from $100^{\circ}C$ to $1000^{\circ}C$. Here, the paste was processed into a powder to measure CIELAB chromaticity, and the samples were taken with a digital camera. The RGB chromaticity was measured by color intensity analyzer software. As a result, the residual compressive strength of W/C=0.5 and 0.3 was 87.2 % and 86.7 % at the heating temperature of $400^{\circ}C$. However there was a sudden decrease in strength at the temperature above $500^{\circ}C$, while the residual compressive strength of W/C=0.5 and 0.3 was 55.2 % and 51.9 % of residual strength. At the temperature $700^{\circ}C$ or higher, W/C=0.5 and W/C=0.3 show 26.3% and 27.8% of residual strength, so that the durability of the structure could not be secured. The results of $L^*a^*b$ color analysis show that $b^*$ increases rapidly after $700^{\circ}C$. It is analyzed that the intensity of yellow becomes strong after $700^{\circ}C$. Further, the RGB analysis found that the histogram kurtosis and frequency of Red and Green increases after $700^{\circ}C$. It is analyzed that number of Red and Green pixels are increased. Therefore, it is deemed possible to estimate the degree of damage by checking the change in yellow($b^*$ or R+G) when analyzing the chromaticity of the fire-damaged concrete structures.

• Journal of Korean Society of Disaster and Security
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• v.14 no.2
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• pp.61-75
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• 2021

Recently, as the occurrence frequency of sudden floods due to climate change increased and the aging of the existing spillway, it is necessary to establish a plan to utilize an auxiliary spillway to minimize the flood damage of downstream rivers. Most studies have been conducted on the review of flow characteristics according to the operation of auxiliary spillway through the hydraulic experiments and numerical modeling. However, the studies on examination of flood damage in the downstream rivers and the stability of the revetment according to the operation of the auxiliary spillway were relatively insufficient in the literature. In this study, the stability of the revetment on the downstream river according to the outflow conditions of the existing and auxiliary spillway was examined by using 3D numerical model, FLOW-3D. The velocity, water surface elevation and shear stress results of FLOW-3D were compared with the permissible velocity and shear stress of design criteria. It was assumed the sluice gate was fully opened. As a result of numerical simulations of various auxiliary spillway operations during flood season, the single operation of the auxiliary spillway showed the reduction effect of maximum velocity and the water surface elevation compared with the single operation of the existing spillway. The stability of the revetment on downstream was satisfied under the condition of outflow less than 45% of the design flood discharge. However, the potential overtopping damage was confirmed in the case of exceeding the 45% of the design flood discharge. Therefore, the simultaneous operation with the existing spillway was important to ensure the stability on design flood discharge condition. As a result of examining the allocation ratio and the total allowable outflow, the reduction effect of maximum velocity was confirmed on the condition, where the amount of outflow on auxiliary spillway was more than that on existing spillway. It is because the flow of downstream rivers was concentrated in the center due to the outflow of existing spillway. The permissible velocity and shear stress were satisfied under the condition of less than 77% of the design flood discharge with simultaneous operation. It was found that the flood damage of downstream rivers can be minimized by setting the amount allocated to the auxiliary spillway to be larger than the amount allocated to the existing spillway for the total outflow with simultaneous operation condition. However, this study only reviewed the flow characteristics around the revetment according to the outflow of spillway under the full opening of the sluice gate condition. Therefore, the various sluice opening conditions and outflow scenarios will be asked to derive more efficient utilization of the auxiliary spillway in th future.