• Journal of Periodontal and Implant Science
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• v.32 no.1
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• pp.143-160
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• 2002

타이태늄은 뛰어난 생체적합성과 적절한 물리적 성질을 바탕으로 치과 및 정형외과 영역의 매식재로 널리사용되어져 왔으며, 골과 매식재 사이의 골 융합 정도를 증가시킬 목적으로 물리, 화학적인 방법을 이용한 타이태늄의 표면처리에 관한 많은 연구들이 진행되어 왔다. 최근에는 부착단백질 또는 성장인자를 이용한 생체재료의 표면개질을 통하여 조직적합성 및 치유 능의 개선을 위한 시도들이 있어왔다. Fibronectin(FN)은 주요 세포외기질중의 하나로 생체 내 널리 분포하여 세포의 부착, 이동 및 증식에 관여하는 거대 당단백으로, RGD및 PHSRN 펩타이드 서열이 세포의 인테그린과 결합하여 세포의 활성을 조절하는 것으로 알려져 있다. 이 연구에서는 FN으로 처리된 타이태늄이 조골세포의 부착, 증식 및 분화에 미치는 영향과 이에 따른 석회화 정도에 미치는 영향을 관찰하여 부착분자를 이용한 타이태늄 표면개질의 효과를 규명하고자 하였다. 상업용 순수 타이태늄을 gold thiol법을 이용하여 표면처리 후, 혈장 FN(plasma FN, pFN)과 유전자재조합법을 이용하여 얻은 FN조각(FN type III 7-10, FNIII 7-10)을 피복한 시편을 실험군으로, 아무런 처리를 하지 않은것(smooth surface, SS)과 산 부식(Sandblasted and acid etched, SLA)처리된것을 대조군으로 이용하였다. 배양된 조골세포주(MC3T3-E1)를 사용하여 타이태늄 표면 처리에 따른 세포의 증식, 형태변화, 알칼리성 인산분해효소(ALPase) 생산 및 세포면역형광법을 이용한 분화정도를 시간 경과에 따라 관찰하였다. 조골세포증식의 경우 FNIII 7-10 처리군에서 pFN 처리군 및 대조군에 비해 시간경과에 따라 유의성있는 세포수의 증식이 관찰되었으며(p<0.05), ALPase 생성의 경우에도 FNIII 7-10 처리 군에서 아무 처리도 하지 않은 군에 비해 유의성 있게 높은 효소의 생성이 관찰되었다(p<0.05). 주사전자현미경을 이용한 세포의 형태관찰결과 아무 처리도 하지 않은 군에서는 마름모형태를 나타내었으며, 산 부식 처리된 군에서는 세포가 가시모양의 형태를 보인 반면 FN으로 처리된 두 군에서는 세포의 부착 및 펴짐이 매우 발달되어 있는 모습이 관찰되었다. 세포의 분화정도를 관찰하기 위하여 국소부착키나제(focal adhesion kinase, FAK), 및 actin stress fiber의 분포양상을 세포면역형광법을 이용하여 관찰한 결과 FN으로 표면처리된 두 군에서 아무런 처리도 하지않은 군 및 산 부식처리 한 군에 비해 프라크의 발현이 높게 나타났으며 잘 발달된 actin stress fiber의 소견을 나타내었다. 이 실험의 결과들은 gold thiol 법을 이용한 표면처리 후 FN부착을 통한 타이태늄의 표면개질이 조골세포의 부착, 증식 및 분화에 중요한 역할을 담당하여 석회화 정도를 촉진시키는 것을 보여주었으며, 이런 결과들은 더 짧은 FN조각을 이용한 다른 생체재료의 표면개질에 폭 넓게 응용할 수 있으리라 생각된다.

• Journal of the Korean Applied Science and Technology
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• v.38 no.2
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• pp.368-377
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• 2021

The present study aimed to evaluate the effects of radiation mutant Perilla frutescens var. crispa and Atractylodes macrocephala Koidzumi complex extract(Perilla frutescens var. crispa complex extract) on the mediators related to degenerative arthritis in a monosodium iodoacetate-induced rat model of degenerative arthritis. Perilla frutescens var. crispa complex extract was administered orally at doses of 25, 50 or 100 mg/kg/day for 2 weeks before direct injection of monosodium iodoacetate (3 mg/50 µl of 0.9% saline) into the intra-articular space of the rats' right knees. The rats subsequently received the same doses of oral Perilla frutescens var. crispa complex extract for another 4 weeks. It was evaluated that the treatment effects based on serum bio-markers, and morphological and histopathological analysis of the knee joints. Compared with those in negative control rats, the Perilla frutescens var. crispa complex extract treatments significantly reduced the serum levels of inflammation, bone metabolism markers (i.e., TNF-α, MMP-3, COX-2, PGE₂, COMP, and Aggrecan). Otherwise, it was significantly increased the production of CTX-2 in cartilage absorption mediators. In addition, the Perilla frutescens var. crispa complex extract treatments effectively preserved the knee cartilage and synovial membrane. As a result, it indicates that the Perilla frutescens var. crispa complex extract improved degenerative arthritis symptoms. Thus, the Perilla frutescens var. crispa complex can be used in food material for the management of degenerative arthritis.

• Archives of Craniofacial Surgery
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• v.11 no.2
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• pp.91-94
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• 2010

Purpose: For the best possible aesthetic reconstruction after craniofacial bone tumor resection, pasteurization has been adopted to devitalize neoplastic cells while maintaining osteoinductive properties and mechanical strength. This case report aims to demonstrate a long-term follow-up result of a monostotic fibrous dysplasia in frontal bone which was reconstructed by pasteurized tumoral autograft in situ. Methods: A 14-year-old girl presented with a hard, nontender, slowly growing mass of 6-year duration on her left supraorbital area. CT showed $5{\times}4{\times}3cm$ sized well defined bony mass confined to frontal bone with heterogeneous density. Tumor was excised completely through bicoronal approach and reimplanted to its original site after pasteurization at $60^{\circ}C$ for 30 minutes. The pathologic examination confirmed fibrous dysplasia. Results: She revisited our clinic 5 years later after suffering some assault on her face. On CT examination, pasteurized tumoral autograft was incorporated to host bone except the fractured upper orbital rim without any evidence of recurrence. She has been satisfied with the result. Conclusion: Pasteurization offers a simple, reliable, cosmetic, economic, and durable reconstruction method for craniofacial skeletal tumor. It has advantages of both biologic incorporation ability and mechanical strength without risk of recurrence. So, it should be considered as one of the primary options in benign as well as resectable malignant tumors of craniofacial skeleton.

• Journal of Periodontal and Implant Science
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• v.33 no.4
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• pp.717-728
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• 2003

This study was performed to evaluate the difference of the bone-to-implant contact according to the surface roughness of the implants. Two beagles were used in the experiment. Extraction of the all premolars was performed in the mandible. In 3 months of healing, screw-shaped pure titanium machined surface implants (Implantium(R), Dentium Co. Korea), implants blasted with 45${\mu}m$ $TiO_2$ particles, (Implantium(R), Dentium Co. Korea) and implants blasted with 100${\mu}m$ $TiO_2$ particles (Implantium(R), Dentium Co. Korea) in diameter 3.4mm and length 6mm were installed in the edentulous mandible. Each dog was sacrificed at 4, 12 weeks after placement and then nondecalcified specimens were prepared for histologic analysis. The results of this study were as follows. 1. At 4 , 12 weeks after the surgery, bone-to-implant contact in the surface blasted with $TiO_2$ particles was higher than that in the pure titanium machined surface respectively. 2. Osseointegration in the surface blasted with 45${\mu}m$ $TiO_2$ particles was more increased than that in the surface blasted with 100${\mu}m$ $TiO_2$ particles. 3. Bone-to-implant contact was increased with time independent of surface roughness. 4. Bone formation was in the outfolded area more than inside the threads independent of surface roughness. From the above results, we were able t o find the most bone-to-implant contact in 45${\mu}m$ $TiO_2$ blasted implant.

• The Journal of the Korean bone and joint tumor society
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• v.12 no.1
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• pp.57-62
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• 2006

Giant cell tumors are potentially malignant tumors in vertebrae, affecting frequently difficult to diagnose and are often inoperable. So it will be treated using radiation because of their high recurrence rate and the mechanical compression of spinal cord, but many surgeons described tumors of the vertebra, and the affected vertebral body can be treated using radical or near to total excision, with anteroposterior vertebral fusion or instrumentation of the spine. we report a case of giant cell tumor affecting the third cervical vertebra which caused neck pain and destroyed the vertebra body had treated using radical excison with fusion of posterior arch using instrumentation of the spine together with a literature review.

• The Journal of the Convergence on Culture Technology
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• v.2 no.4
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• pp.1-21
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• 2016

This article examined the different types of Jeonuhwa as recorded in 16 royal palace studies from the Joseon dynasty (1392-1909). The ingredients used in Jeonuhwa during the Joseon dynasty were categorized as follows: 16.0% for gray mullet(秀魚) and fish(生鮮), 14.2% each for liver and cow stomach, 12.3% for sea cucumber(海蔘), 4.7% each for crab(蟹), clam(生蛤), and chunyup(千葉), 3.9% each for pork meat(豬肉), octopus, 2.8% each for oyster(石花), pheasant(生雉), and chicken(鷄), 2.0% each for croaker(民魚), and brain (骨), 0.9% each for duck(鴨子), pigeon(山鳩), dobi(都飛), snapper, white fish(白魚), mussel(紅蛤), haeran(蟹卵), quail(鶉鳥), egg(鷄卵), and sesame(實荏子). This observation may be associated with commercial industrial development that prevailed during the late Joseon dynasty. Further studies will be conducted on recipes and ingredients recorded in Euigwe in order to develop a standardized recipe for Jeonuhwa.

• Applied Microscopy
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• v.1 no.1
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• pp.35-42
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• 1969

가토간장에서 채취(採取)한 간(肝)디스토마 성충(成蟲)의 정소(精巢)와 수정낭(受精囊)을 1.25% glutaraldehyde와 1% 사산화(四酸化) 오스뮴산(酸)으로 냉실(冷室)에서 이중고정(二重固定)하였다. 고정(固定)된 시료(試料)는 양식(樣式)에 따라 탈수(脫水)한 후(後) Epon-812로 포매(包埋)하여 MT-2형(型) Porter Blum microtome으로서 초박절편(超薄切片)을 만들어 수사화연(水酸化鉛)과 초산(醋酸)우라닐로서 이중염색(二重染色)한후 Hitachi HS-7형(型)과 HU-11E형(型)의 전자현미경(電子顯微鏡)으로 관찰(觀察)하였다. 관찰결과 간(肝)디스토마의 정세포(精細胞)는 타원형으로 난형(卵形)의 인(仁)을 포함(包含)한 큰 핵(核)을 갖고 있으며 핵(核)을 둘러싼 비교적소량(比較的小量)의 세포질(細胞質)에는 낭형(囊形)의 조면소포체(粗面小胞體)가 희소(稀少)하게 있으며 유리(遊離)리보좀 및 즐(櫛)을 가진 미토콘드리아, 중심체(中心體), 층판상(層板狀)의 골지체가 있다. 정자(精子)는 긴 원주형(圓柱形)의 핵(核)과 선단(先端)에 첨체(尖體)를 포함(包含)한 두부(頭部)와 중종편(中終片), 미부(尾部)의 말단부(末端部)로 갈수록 점차 가늘어져서 결국(結局) 편모(鞭毛)로 끝난다. 두부(頭部)의 선단부(先端部) 가까이 핵환(核環)이 있으므로 이를 기시점(起始點)으로 직경(直徑) $250{\AA}$ 정도의 $8{\sim}10$개(個)의 미세소관(微細小管)이 축사(軸絲)의 배면(背面) 원형질막(原形質膜) 직내면(直內面)의 외형질(外形質)에 평행(平行)하게 중종편(中終片)까지 신장(伸長)되어 있다. 미토콘드리아는 융합(融合)되어 두부(頭部)의 후반부(後半部) 핵(核)의 복측(腹側)에 평행(平行)하게 축사(軸絲)를 감싸는 원추형(圓錐形)으로 종단면(縱斷面)에서 반점상(班點狀)의 횡문(橫紋)이 관찰(觀察)되었다. 중심체(中心體)에서 기원(起原)된 축사(軸絲)는 중심부(中心部)에 중심섬유(中心纖維)와 중심초 및 이중(二重)의 미세소관(微細小管)이 9개(個) 둘러싸고 있는 구조(構造)를 하고 있다.

• The korean journal of orthodontics
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• v.34 no.5 s.106
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• pp.458-464
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• 2004

Anchorage plays an important role in orthodontic treatment especially in the maxillary arch. In spite of many efforts for anchorage control. it was difficult for clinicians to predict the result of treatment because most of the treatment necessitated an absolute compliance of patients, But recently, skeletal anchorage has been used widely because it does not necessitate patient compliance but produces absolute anchorage. In addition titanium miniscrews have several advantages such as ease of insertion and removal. possible immediate leading and use in limited implantation spaces. In this case, a skeletal Class I bialveolar protrusion Patient was treated with standard edgewise mechanics using indirect active P.S.A. (palatal skeletal anchorage). The miniscrews in the paramedian area of the hard palate provided anchorage for retraction of the upper anterior teeth and remained firm and stable throughout treatment This indicates that the PSA can be used to reinforce anchorage for orthodontic treatment in the maxillary arch Consequently, this new approach can help effective tooth movement without patient compliance, when used with various transpalatal arch systems.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.24 no.3
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• pp.188-193
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• 2011

The osseointegration of dental implant is influenced by many factors such as surface geometry, loading and the amount of bone. Thus, stability of the dental implant should be checked periodically. In order to test the stability of dental implant by using resonance frequency analysis, we designed a structure of transducers and fabricated a piezoelectric devices. Using finite element analysis, the thickness and length of piezoelectric device and transducers were tailorized and the optimized frequency of 10 kHz was obtained. The resonance frequency from simulation analysis and evaluation was estimated to be similar as 10 kHz. The osseointegration was further enhanced with increasing frequency from the evaluation result of the finite element analysis.

• Journal of Genetic Medicine
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• v.7 no.2
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• pp.151-155
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• 2010

Apert syndrome is a rare congenital anomaly characterized by craniofacial malformations and severe symmetrical syndactyly of fingers and toes. This syndrome is caused by a genetic mutation; the S253 mutation is common, though the P253R mutation is not as frequent. Common symptoms include skeletal malformations, poor joint mobility, eye and ear problems, cleft palate, and orthodontic and other dental problems. We report a case of an infant with the common morphological features of Apert syndrome. Interestingly, she was found to have the P253R mutation in FGFR2 exon VIII, which has been less commonly observed in Korea. A brief review of the literature is included.