• Proceedings of the Korean Society of Applied Pharmacology
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• 1992.05a
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• pp.16-16
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• 1992

1940년대에 처음으로 암의 화학요법이 시도된 이래 많은 항암제들이 도입되어 사용되고 있다. 현재 항암제를 사용하여 비교적 치료율이 높은 암은 leukemia나 lymphoma와 같은 혈액암에 국한되어 있다. 고형암에 대하여는 고환암과 같은 극히 일부가 항암제에 의해 비교적 잘 치료되나 대부분은 여전히 치료가 되지 않고 있는 상태에 있다. 고형암의 발생빈도는 전체 암의 90％를 차지하고 있는 상태로 사망율이 매우 높아, 현대의 40대 이상 사망원인의 1위를 나타내고 있어 그 치료제의 개발이 매우 시급히 요구되고 있다. 이에 따라 고형암의 치료를 목적으로 많은 화합물들이 검토되어 왔다. 이중 최근 매우 큰 가능성을 보여주고 있는 diarysulfonylurea 유도체가 발견되어 임상시험에 도입됨으로서 이 화합물들에 대한 관심이 고조되고 있다. 이와 관련하여 고형암의 치료제를 개발할 목적으로 diarysulfonylurea의 특성과 강력한 항암효과를 발현하는 alkylating agent의 특성을 갖을 것으로 예상되는 새로운 2-alkoxy-1-arylsufonylimidazoline 유도체를 설계하고, 이들 유도체의 합성법을 개발하였다. 이들 유도체들은 일차적으로 생체내에서 DNA 또는 효소등에 alkylation을 일으킨후 diarysulfonylurea로 변하여 작용함으로서 보다 큰 항암효과를 발현할 것으로 기대된다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.1
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• pp.59-65
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• 2004

Hot water-extracts prepared from Cordyceps militaris of silkworm pupa (CMP) or Cordyceps militaris of silkworm larva (CML) were tested for tumor growth inhibitory and immunomodulatory activities in ICR mice bearing sarcoma-180 cells solid tumor, and compared with those of the known compound, cordycepin, found in Cordyceps militaris. Mice were subcutaneously injected with sarcoma-180 cells, and i.p. injected with either saline (Control), 50 mg/kg or 100 mg/kg of CMP (CMP50 or CMP100, respectively), or CML (CML50 or CML100, respectively), or 1 or 2 mg/kg of cordycepin (C1 or C2, respectively) for 10 days. Mice injected with CMP50 or CMP100 showed a 47.3% or 57.6% inhibition in the solid tumor growth (P<0.05), while those injected with CML50 or CML100 exhibited a 35.5% or 37.1% reduction (p<0.05) in solid tumor size compared to the value for control mice treated with saline. Animals injected with corcycepin showed a 26∼30% inhibition in the solid tumor growth (P<0.05). Mice bearing solid tumor and injected with CMP or CML showed a significantly increased thymus weight (38∼44% increase), lymphocyte percentages of CD4+ T-cell, CD8+ T-cell, and NK-cell (63∼110% increase) in the spleen, and interleukin-2 excretion (33∼51% increase) by the isolated splenocytes compared to those in control mice (p<0.05). These results indicate that the anti-tumor activity of hot water extracts of Cordyceps militaris, raised on both silkworm pupa and silkworm larva, appears to be associated with their immunomodulatory activity, and these activities found in Cordyceps militaris are superior to those for the single compound, cordycepin.

• Journal of Korean Biological Nursing Science
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• v.8 no.2
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• pp.5-12
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• 2006

목적 : 본 연구는 암환자 및 암의 유형에 따라 중요한 종양억제 조절인자로 알려져 있는 모노카인을 flowcytometry를 이용하여 분석, 비교하고자 하였다. 방법 : 연구대상은 고형종양(solid tumor)으로 진단받은 33세에서 76세 사이의 암환자 30명(유방암, 난소암, 폐암, 위암)을 대상으로 말초혈액 단구의 intracellular monokine 중 $TNF{\alpha}$, MIG, MIP를 분석한 유사실험설계 연구이다. 연구결과 : 암환자 군에서의 $TNF{\alpha}$, MIG, MIP 수치는 대조 군인 정상 군에 비해서 유의하게 증가되었으며 특히, 유방암과 난소암 환자 군에서의 $TNF{\alpha}$ 수치는 폐암과 위암의 대상자에 비해 의미 있는 차이를 보여주었다. 논의 : 본 연구에서 제시된 암환자 군에서의 모노카인 수치는 선행연구의 결과와 통일하게 종양 대상자의 면역에 중요한 역할을 하는 것으로 규명 되었으나, $TNF{\alpha}$는 고형종양 중에서도 여성생식기계 암환자 군에서 더 증가하였다. 이에 따라 종양 유형에 따른 모노카인의 역할과 호르몬과의 상호작용기전 규명에 대한 추후 연구가 필요하다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.10
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• pp.1520-1524
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• 2005

Young radishes (YR, yeolmu in Korean) were cultivated in the soil with and without sulfur. Young radish kimchi-general (YR kimchi-G) was prepared with YR commonly cultivated in the soil without sulfur. Young radish kimchl-sulfur (YR kimchi-S) and young radish kimchi-sulfur with lime mortar (YR kimchi-SL) were prepared with the YR cultivated in the soil with sulfur an4 sulfur added lime mortar on it respectively. Antitumor effects of methanol extracts from the YR kimchis were investigated in sarcoma-180 tumor cell transplanted mice. The solid tumor growth was significantly inhibited by the YR kimchi-SL prepared with YR grown in the soil with sulfur (p < 0.05). The treatment of the methanol extracts from YR kimchi-S and -SL increased the glutathione S-transferase activities and glutathione contents in the livers, compared to thlose of YR kimchi-G and the control. One of the antitumor effects by the YR kimchis was due to the increased the glutathione levels and the glutathione S-transferase activity which is phase 2 enzyme. These results also suggested that the antitumor effects of YR kimchi can be enhanced by using YR cultivated differently in the presence of sulfur that can help to produce sulfur-containing compounds in YR.

• Journal of Life Science
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• v.20 no.11
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• pp.1738-1741
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• 2010

Caliper measurements of tumor volume have been widely used in the assessment of tumors in animal models. However, experiments based on caliper data have resulted in unreliable estimates of tumor growth, due to necrotic areas of tumor mass. To overcome this systematic bias, we engineered a new luciferase-expressing rat prostate cancer cell line (MLL-Luc) that produces bioluminescence from viable cancer cells. MLL-Luc cells showed a strong correlation between bioluminescence intensity and cell number ($R^2$=0.99) and also accurately quantified tumor growth, with reduced bioluminescence signals caused by necrotic cells in a subcutaneous MLL-Luc xenograft model. The accurate quantification of tumor growth with bioluminescence imaging (BLI) was confirmed by a better antitumor effect of combination chemotherapy, compared to that based on caliper measurements with a correlation between the bioluminescence signal and tumor volume ($R^2$=0.84). These data suggest that bioluminescent MLL xenografts are a powerful and quantitative tool for monitoring tumor growth and are useful in evaluating the efficacy of anticancer drugs, with less systematic bias.

• Radiation Oncology Journal
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• v.17 no.4
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• pp.281-286
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• 1999

Purpose : Brain metastases are the most frequent metastatic neurologic complication of systemic cancer. Even if the prognosis of brain metastases is grave, with available treatments, most patients experience effective palliation of neurologic symptoms and meaningful extension of life. We evaluated the clinical features and prognostic factors of the patients who were diagnosed as solid tumors with brain metastasis and received radiotherapy for brain metastases. Materials and Methods: Between January 1987 and January 1998, 71 patients with brain metastases from solid malignancy were included. We reviewed neurologic symptoms and signs of patients and evaluated improvememt of neurologic symptoms and signs. Survival durations after brain metastasis were ana lysed according to several factors such as age, performance status, primary malignancies, the pre-sence of brain metastasis at initial diagnosis of primary tumor, multiplicity of brain metastass, the presence of metastases other than brain, and treatment method. Results: Frequent symptoms associated with brain metastasis were headache (47.9$\%$), motor weakness (40.8$\%$), nausea and vomiting (19.7$\%$) and mental change (19.7%). Palliation of these symptoms was accomplished in 64.9$\%$ of cases. The overall median survival time was 16 weeks and 1- and 2-year survival rates were 15.0$\%$ and 5.1$\%$ respectively. Patients without extracranial metastases (n=27) had longer median survival than patients with extracranial metastases (33 weeks vs 10 weeks, p=0.0018). In patients with single brain metastasis (n=37), the median survival time was longer in patients treated with surgery plus radiotherapy than in patients treated with radiotherapy alone (40 weeks vs 16 weeks, p=0.0438). Conclusion: Patients who has brain metastases only constitute a prognostically favorable group and they may be benefited from radiotherapy and surgery if indicated.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.241-241
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• 1994

괄루근과 포공영으로 부터 수용성 다당류를 분리하고 제단백 및 DEAE cellulose column을 이용한 정제과정을 거쳐 얻은 렉틴성 다당류를 시료로 하여 면역기능에 미치는 효과와 고형암에 대한 성장억제효과를 검토하여 얻은 결과는 다음과 같다. 시료 렉틴류는 혈중 백혈구수와 총복강세포수를 현저히 증가하며 이러한 현상은 포공영의 경우가 보다 강하며 또한, 1주일의 한도내에서 시간이 경과할수록 뚜렷하였고 총복강세포의 수는 제1일에는 포공영의 경우에 특히 크게 증가하였으나 시간이 경과할수록 정상치 가까이 회복하였다. 과민성에 미치는 영향을 검토한 실험에서는 암세포이식으로 인해 항체생산능 및 T lymphocyte reactivity가 크게 저하되었으나 시료의 투여로 현저한 회복현상을 보였다. 고형암 성장 억제 실험에 의하면 5mg/kg의 낮은 투여량에서 성장억제도는 현저하며 이러한 현상은 정제의 단계가 진행될수록 커졌다.

• Proceedings of the Korea Contents Association Conference
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• 2019.05a
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• pp.469-470
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• 2019

차세대염기서열분석법(NGS, Next Generation Sequencing) 기술은 하나의 유전체를 무수히 많은 조각으로 분해하여 각 조각을 동시에 읽어낸 뒤, 전산기술을 이용하여 조합함으로써 방대한 유전체 정보를 빠르게 해독하는 방법이다. 한편, 액체 생검(LB, liquid biopsy)이란 암세포가 깨지면서 생기는 미량의 DNA 조각을 말초혈액 속에서 찾아내 암을 진단하는 기술로 조직 생검(tissue biopsy)에 비해 비침습적이다. 본 논문은 NGS와 LB 기술을 접목했을 때 확진이 가능하고 예후 및 치료경과의 예측이 가능함을 제언하였다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.4
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• pp.424-430
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• 2007

This study was conducted to investigate the effect of mycelia of Lentinus edoes mushroom-cultured Lonicera japonica Thunberg (LLJ) on proliferation of the cancer cell lines (Hep3B, MCF-7 and HeLa), sarcoma 180 (S-180) and antiallergy. In an anti-cancer test using Hep3B (hepatic cancer cell), MCF-7 (breast cancer cell) and HeLa (uterine cancer cell), LLJ extract showed higher antiproliferating effect than that of LJ (Lonicera japonica Thunberg) extract. In an anti-cancer testing using Hep3B cells, LLJ extract showed growth-inhibitory effect of $85.60{\pm}4.66%$ at 3mg/rnL. In an anti-cancer testing using MCF-7 cells, LLJ and LJ extracts showed high antiproliferating effect. LLJ showed the tumor suppressive effect in mice injected with S-180 cells. The growth-inhibitory rates against tumor cells were 61% for LLJ, 37% for LJ. LLJ inhibited histamine release from rat peritoneal mast cells activated by compound 48/80. These results suggest that Lentinus edodes mushroom-cultured herb has an antiproliferating effect against cancer cell lines (Hep3B, MCF-7 and HeLa) and S-180 tumor, and will be beneficial in the treatment of allergic reaction.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.1
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• pp.8-13
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• 2007

This study was conducted to investigate the effect of mycelia of Lentinus edoes mushroom-cultured Astragalus membranaceus Bunge (LAM) on proliferation of cancer cell lines (Hep3B, MCF-7 and HeLa), sarcoma 180 (S-180), and anti-allergy. In an anti-cancer test using Hep3B (hepatic cancer cell), MCF-7 (breast cancer cell) and HeLa (uterine cancer cell), LAM extract showed higher antiproliferating effect than that of Astragalus membranaceus Bunge (AM) extract. In an anti-cancer testing using Hep3B cells and MCF-7 cells, LAM extract showed growth-inhibitory effect of 65.23% at 3 mg/mL and 69.23% at 5 mg/mL, respectively. In an anti-cancer testing using HeLa cells, LAM extract showed growth-inhibitory effect of 42.01% at 5 mg/mL. In addition, LAM showed the tumor suppressive effect in mice injected with S-180 cells. The growth-inhibitory rates against tumor cells were 47% for LAM and 37% for AM. LAM inhibited histamine release from rat peritoneal mast cells activated by compound 48/80. These results suggest that Lentinus edodes mushroom-cultured herb has an antiproliferating effect against cancer cell lines (Hep3B, MCF-7 and HeLa), S-180 tumor and will be beneficial in the treatment of allergic reaction.