• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.8
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• pp.417-424
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• 2019

Despite its superior ability to show distinct cellular morphology and for long-term storage, conventional tissue fixation by formalin has many drawback, including slower fixation, the exposure to harmful chemicals and extensive protein modification. Herein, we assessed the effects of rapid microwave-assisted tissue fixation on histological examination and on protein integrity by comparing these microwave irradiation fixated tissues with the formalin-fixed tissues. One of the paired mouse tissues (liver and kidney) was fixed in formalin and the other was fixed by using microwave irradiation in phosphate buffered saline. Each slide from the paraffin-embedded tissues was examined by H & E staining for the adequacy of fixation and by immunohistochemical staining for antigenicity in a blinded fashion. Evaluation of protein recovery and the protein quality from the fixed tissues were analyzed by the BCA method and Western blotting, respectively. The results from H & E staining and immunohistochemical staining showed that the sections obtained from microwave-fixed tissues under our experimental conditions were comparable to those of the formalin-fixed tissues except for the integrity of RBCs. Furthermore, proteins were effectively extracted from the microwave-fixed tissues with acceptable preservation of the proteins' quality. Taken together, this microwave-assisted tissue processing yields a quick fixation and better protein recovery in higher amounts, as well as the adequacy of fixation and the antigenicity being comparable to formalin-fixed tissues, and this all suggests that this new fixation technique can be applied in an environment where rapid tissue fixation is required.

• Journal of the Korean Society of Radiology
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• v.17 no.1
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• pp.115-122
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• 2023

The hip joint device is an important immobilization device for internal rotation in the diagnosis of bone density test, microfracture, and arthritis. These fixing mechanisms have limitations in maintaining reproducibility due to the use of Velcro. In this study, we developed an improved hip joint device and evaluated its usefulness. For 30 students enrolled in the Department of Radiology, we evaluated the reproducibility through medical imaging and the time required for positioning by applying the conventionaling and improved device. Changes over time were also evaluated. As a result, compared to the conventional device, the improved device showed a 32% reduction in positioning time and improved reproducibility by about 6.2 times. As for the amount of change over time, the positioning time increased in the conventional device, but decreased in the improved device, and the reproducibility of the conventional device was lower than that of the improved device. Through this, it can be said that the use of the improved device improved the image quality and reduced the radiologist's workload.

• Proceedings of the KIEE Conference
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• 2003.07c
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• pp.1935-1937
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• 2003

최근 콜레스테롤 센서는 전극 상에 효소를 고정화 하는 방식을 이용하여 센서의 집적도를 높이는 시도가 이루어지고 있다. 이러한 전극 상의 효소고정화 방식으로 entrapment, cross liking, covalently binding 등이 있다. 본 논문에서는 이러한 효소 고정화 방식-전도성 고분자인 P3MT를 사용하여 entrap시키는 방법과 silanization을 이용한 covalent bonding 시키는 방법-에 따른 전기화학 센서의 감도 특성에 관한 연구를 수행하였다. 전도성 고분자를 사용한 고정화 방법은 cyclic voltammograms으로 scan rate 10 mA/s, potential 0.5-1.3V의 조건하에서 P3MT를 Polymerization하고, 효소 고정화를 위해 chromoampermeter로 potential 0.6V에서 900초 동안 수행하였다. silanization을 이용한 covalent bonding 시키는 방법은 nitric acid로 Pt 전극표면을 산화시키고, APTER로 silanization 공정을 시행하였다. 효소 고정화를 위해 전해질로는 0.1M Phosphate buffer solution을 사용하여 cyclic voltammograms으로 scan rate 50 mA/s 전위 0.0-0.7V의 조건 하에서 수행하였다. 이 결과 전도성 고분자를 이용한 고정화 방법에서의 senstivity가 0.89 ${\mu}A/mM{\cdot}cm^2$이고, silanization을 이용한 효소 고정화 방법에서는 1.51 ${\mu}A/mM{\cdot}cm^2$였다. 이처럼 후자의 방법에서 더 좋은 감도 특성이 나타났다. 따라서, silanization을 이용한 고정화 방법이 센서 제작 방식으로 더 적합하다고 사료된다.

• Composites Research
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• v.20 no.5
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• pp.7-12
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• 2007

This paper aims to enhance the efficiency of bone fracture healing with bone plate made of fiber reinforced composite materials. The composite bone plate was designed as the same dimension and shape as the existing stainless steel bone plate. To find out the appropriate stacking sequence of the composite bone plate the variations of strain distributions were calculated using FE analysis when the bone plates were applied to the fracture site. From the analysis result it was found that the composite bone plate whose Young's modulus is lower than that of metal bone plate gave more uniform strain distribution and provided appropriate condition for callus formation and its development.

• Proceedings of the KIEE Conference
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• 2008.04a
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• pp.43-44
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• 2008

본 논문에서는 Weighted Cenrtoid Localization(WCL) 알고리즘을 적용하여, 고정 노드 (Anchors) 사이의 측정된 수신 신호 강도(RSSI)로 Zigbee 실내 위치 인식 시스템을 구현하였다. 센서 노드의 위치는 RSSI에 의해 선택된 가상 고정 노드에 WCL을 적용하여 결정한다. WCL에서 부여하는 가중치는 고정 노드와 RSSI 센서 노드 위치의 거리에 대한 함수이며, 고정 노드 간의 측정된 RSSI 값으로 구한다. 고정 노드 간의 RSSI로 가상 고정 노드를 이용한 WCL은 기존의 방식에서 사용되는 고정 노드의 수를 줄이고, 측정 대상의 위치 정확도를 측정된 RSSI 값만으로 추정한 위치보다 7.8% 향상되는 것을 확인 하였다.

• Proceedings of the Korea Information Processing Society Conference
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• 2009.04a
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• pp.1186-1189
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• 2009

본 논문에서는 이동 애드 혹 네트워크의 성능 개선을 위한 방안으로 네트워크 중간에 고정 노드를 부가하여 어느 정도의 성능 향상이 있는지를 모의 실험을 통하여 평가하였다. 모의 실험 결과 이동 노드만을 사용했을 경우보다 고정노드를 함께 사용했을 때의 성능이 더 뛰어남을 알 수 있었고, 이동 노드의 이동 속도에 따른 비교에서도 고정 노드를 함께 사용한 경우가 성능이 더 뛰어남을 확인할 수 있었다. 또한 고정 노드의 개수에 따른 성능평가에서는 고정 노드를 여러 대 사용할수록 지연시간이 단축됨을 확인 할 수 있었다. 본 연구에서 제안한 방식은 이동 애드 혹 네트워크의 중간에 고정 노드를 부가하여야 하는 문제가 있으나, 비용과 처리율과의 관계를 비교한 후 비용 상승에도 불구하고 보다 높은 신뢰성과 빠른 처리율을 원하는 네트워크에 사용하면 효과적일 것이다. 향 후 본 절의 연구 결과에 이어서 애드 혹 네트워크에 몇 대의 고정 노드를 추가해야 하는지에 대한 연구가 이루어진다면 보다 높은 신뢰성을 가진 애드 혹 네트워크가 구축될 수 있을 것이다.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.46 no.2
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• pp.71-77
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• 2009

A novel phase-locked loop(PLL) architecture with multiple charge pumps for fast locking has been proposed. The proposed PLL has three charge pumps. The effective capacitance and resistance of the loop filter can be scaled up/down according to the locking status by controlling the direction and magnitude of each charge pump current. The fast locking PLL that changes its loop bandwidth through controlling charge pumps depending on locking status has been designed. The capacitor usually occupying the larger portion of the chip is also minimized with the proposed scheme. Therefore, the PLL size of $990{\mu}m\;{\times}\;670{\mu}m$ including resistors and capacitors at the bandwidth of 29.9KHz has been achieved. It has been fabricated with 3.3V $0.35{\mu}m$ CMOS process. The locking time is less than $6{\mu}s$ with the measured phase noise of -90.45dBc/Hz @1MHz at 851.2MHz output frequency.

• KSBB Journal
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• v.18 no.4
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• pp.340-345
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• 2003

Feasibility of large-scale photobioreactors for biological CO$_2$ fixation was investigated using Chlorella sp. HA-1. Generally, as the volume of photobioreactor increased, the CO$_2$ fixation rate decreased because of a lower illumination efficiency in large-scale than in small-scale photobioreactors. Though controlling the arrangement and the number of light source, the maximum CO$_2$ fixation rates that could be achieved were 530 and 357 gCO$_2$/㎡day for 40 L and 188 L photobioreactor, respectively, which was higher than the CO$_2$ fixation rate of lab-scale photobioreactor.

• KSBB Journal
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• v.15 no.1
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• pp.42-48
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• 2000

Urokinase (UK), a thrombolytic enzyme used to clear catheters obstructed by blood clots, can be also used industrially in the recombinant protein purification system to cleave a fusion protein linked with a certain fragment of GST. We have immobilized UK by covalent attachment to activated Sepharose 6B-Cl gels and evaluated its performance to cleave a fusion protein of hGH and GST. The Sepharose gels were activated by etherification with glycidol (2,3-epoxypropanol) and further oxidized with periodate resulting in glyceryl-Sepharose gels. After the activation treatment, surface density of the aldehyde groups was 7-30 $\mu$mol-aldehde/mL-gel. Immobilization yield was higher than 99% at high pH (10.5), and the immobilized UK maintained ca. 80% specific activity of the soluble UK. In a column reaction the cleavage yield heavily depended on the feed rate, and it was nearly 86% of that from soluble UK. And the immobilized UK was successfully regenerated by unfolding and refolding with 6M GuHCl. After cleavaging reaction, the monomeric hGH was purified by using expanded bed adsorption chromatography.

• Korean Journal of Social Welfare Studies
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• v.44 no.1
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• pp.89-111
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• 2013

This study set out to investigate factors affecting gender-role stereotype. Data came from National Welfare Panel Study of 2010 and the final sample size were 4,196 women and 3,703 men in Korea. Hierarchical regression analysis was used for the analysis. The major findings of this study are as follows. First, the olders, the married and the lower level of income showed more traditional gender-role attitudes. Second, the employed women have more egalitarian gender-role attitudes than the not-employed but the employed men showed more traditional gender-role attitudes. And the more educated women showed more egalitarian gender-role attitudes but men's gender-role attitudes had no difference according to the education level. Third, self-esteem did not affect gender-role attitudes. And limitations and implications of the findings from this study were discussed with respect to further studies.