• Title/Summary/Keyword: 고정조사

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Optimal Material and Conditions for the Immobilization of Rhizopus nigricans in the $11{\alpha}-Hydroxylation$ Reaction of Progesterone (Rhizopus nigricans를 이용한 Progesterone의 $11{\alpha}-Hydroxylation$ 반응에서의 고정화 재료와 조건의 최적화)

  • Kim, Myung-Hee;Lee, Jung-Jin;Kim, Mal-Nam;Min, Byung-Re
    • The Korean Journal of Mycology
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    • v.18 no.2
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    • pp.84-88
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    • 1990
  • Hydroxylation in the $11{\alpha}$-position of progesterone molecules was carried out using Rhizopus nigricans spores immobilized within various gel matrices, among which polyacrylamide and agar gel were proved to be the most effective. Reactions with the immobilized cells and in­tact free cells showed almost identical conversion rate of progesterone, optimal pH and reaction time for attaining maximal yield, from which were confirmed absence of any decay and modification of enzyme activities.

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Continuous Production of γ-aminobutyric Acid by Immobilization of Lactobacillus brevis (Lactobacillus brevis의 고정화 균체에 의한 γ-aminobutyric acid의 연속 생산)

  • 류병호;전재호
    • Journal of Life Science
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    • v.14 no.1
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    • pp.167-173
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    • 2004
  • The optimal conditions for the continuous production of $\gamma$-aminobutyric acid by immobilization of Lactobacillus brevis BH-21 using column type reactor were investigated. The optimal conditions of operation were 2.2 mm diameter bead of 3.0% sodium alginate at 10 mL/h of substrate feeding rate. Continuous production by immobililzed cells showed the highest productivity with replacement of fresh medium in every 48h for fourth fermentatoin cycle following the rendition of $\gamma$-aminobutyric acid productivity. A productivity of $\gamma$-aminobutyric acid could be obtained for 25 days by continuous column type reactor under optimal conditions.

Dispersion Indices and Sequential Sampling Plan for the Citrus Red Mite, Panonychus citri (McGregor) (Acari: Tetranychidae) on Satsuma Mandarin on Jeju Island (온주밀감에서 률응애의 공간분포분석 및 표본추출법)

  • 송정흡;이창훈;강상훈;김동환;강시용;류기중
    • Korean journal of applied entomology
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    • v.40 no.2
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    • pp.105-109
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    • 2001
  • Dispersion pattern of the citrus red mite (CRM), Panonychus citri (McGregor) was determined to develop a monitoring method in the satsuma mandarin fields, Citrus unshiu L., in Jeju-do, during 1999 and 2000. CRM population was sampled by collecting leaves. Taylor's power law provided better description of mean-variance relationship for the dispersion indices compared to Iwao's patchiness regression. Slopes and intercepts of Taylor's power law from leaf samples did not differ among surveyed groves. Fixed-precision levels (D) of a sequential sampling plan were developed using Taylor's power law parameters generated from all motile stages of CRM in leaf sample. This sampling plan for leaf sample estimate was tested with resampling validation for sampling plan using 4 independent data sets. Resampling simulation analysis demonstrated that actual fixed-precision level values were better than desired D values of 0.20, 0.25 and 0.30. Required numbers for tree sampling at the density of more than 7 mites per tree were fewer than 18.

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Geotechnical Characterization of the Eardo Seabed for Offshore Pile Foundation Design (해양말뚝 기초설계를 위한 이어도 해저지반의 특성화)

  • Shim, Jae-Seol;Yoon, Gil-Lim;Kown, O-Soon
    • Journal of the Korean Geotechnical Society
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    • v.15 no.5
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    • pp.141-155
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    • 1999
  • Korea Ocean Research & Development Institute(KORDI) conducted an offshore geotechnical investigation for the Eardo Ocean Research Station with the help of the Fugro International Limited at a site location approximately 152 km away from Mara Island, Korea. The primary purpose of the geotechnical investigation was to obtain information on soil and foundation conditions, and to develop foundation design data for a fixed offshore observation platform. This paper discussed the details of the geotechnical investigation and the foundation design recommendations for the Ocean Research Station. Clear recommendations were proposed for the foundation type of driven pile considering the existing soil conditions.

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CERN이 제안한 미임계 평형 원자로에 대한 소고

  • 노태완;이지영;이재중
    • Proceedings of the Korean Nuclear Society Conference
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    • 1997.05a
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    • pp.96-101
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    • 1997
  • 양자가속기를 외부 중성자 공급원으로 하여 미임계 운전을 가능하게 하고 토륨을 핵연료로 사용하므로 장주기 핵종과 핵무기 재료물질의 발생량을 현저히 줄일 수 있는 새로운 노형인 energy amplifier에 대한 연구가 CERN을 중심으로 활발히 진행되고 있다. 본 연구에서는 토륨주기에 대하여 고정 중성자속 조사에 의한 핵분열 및 방사붕괴에 관한 모델을 정립하여 다수의 연립선형 미분방정식으로 구성하여 Runge Kutta 5-6차 자동시간 간격 수치해법을 이용하여 계산하였다. 결과는 1014의 고정 중성자속에 대하여 충분한 U233의 생산이 평형상태에 도달하고 장주기 핵종도 우라늄 주기에 비하여 현저히 줄어듬을 보이므로 가속기를 이용한 토륨 핵연료 주기의 타당성을 확인하였다.

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전동싸리의 생장에 미치는 Al과 Co의 영향

  • 박태규;송승달
    • Proceedings of the Korean Environmental Sciences Society Conference
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    • 2000.05a
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    • pp.245-246
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    • 2000
  • 콩과식물인 전동싸리를 대상으로 통제된 조건하에서 Al과 Co처리에 따른 식물체의 생장반응과 식물체내 중금속의 축적정도 및 근류의 질소고정 활성의 변화를 조사한 결과 중금속은 Co가 Al보다 높게 축적되었고, 처리 초기에는 지하부를, 장기간 처리시는 광합성 기관의 생장을 억제했는데, Co 처리는 영향이 미약하였으나, Al처리구는 다소 심각한 영향을 주었으며, 생장에 비해 질소고정계의 피해가 현저하였다. 전동싸리는 흡수된 중금속을 뿌리수준에서 조절함으로써 광합성기관으로 이동을 억제하는 중금속에 대한 생장적응을 나타내었다. 결국 전동싸리는 30ppm의 중금속 처리시 뿌리 수준에서 조절함으로써 광합성기관으로 전이를 차단하여 금속에 대한 내성 나타내었고, Al이 Co보다 심각한 영향을 미치는 것으로 나타났다.

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褪變檔案字迹的恢復与保護

  • 이옥호
    • Journal of Korean Society of Archives and Records Management
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    • v.2 no.2
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    • pp.205-211
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    • 2002
  • 퇴변한 필적을 회복하고 보호하는 것에 관계되는 여러 학과를 설립하여, 각고의 연구 끝에 이 영역에서 일련의 중대한 돌파를 얻었다. 볼펜 복사지 푸른색 먹(黑) 연필 청사진 등의 필적 재료의 퇴색 확산 회복 고정기제 및 종이 노화와 보호기제 등 복잡한 요소를 연구 분석하여 일련의 퇴변한 당안 필적 회복과 보호제를 연구 제작하였다. 이 일련의 성과가 세상에 나온 이래 중국 30개성 자치구에서 정부기관 경찰 법원 도시규획과 건설 지질 수도 전기 철로 석유 조사 설계 군사 등의 일부분 당안에서 응용되었고, 심각하게 퇴변한 대량의 진귀한 문헌 문물로 하여금 원래 모습을 회복시켰으며, 영구 보존할 필요가 있는 문건을 내구적으로 고정시킬 수 있었다. 동시에 국제 당안계 및 지질문물 수장, 연구 부분에서 강렬한 영향을 불러일으켰다.

The Production of Lincomycin by Repeated Batch Cultures of Immobilized Streptomyces lincolnensis (고정화된 Streptomyces lincolnensis의 반복 회분식 배양에 의한 린코마이신 생산)

  • Kim, Chang-Joon;Chun, Gie-Taek;Chang, Yong-Keun;Kim, Sung-Bae
    • KSBB Journal
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    • v.21 no.5
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    • pp.384-388
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    • 2006
  • The production stability of high-yielding mutants of Streptomyces lincolnensis immobilized on celite beads was examined in repeated batch cultures. We also explored the feasibility of immobilization of vegetative mycelial cells on pre-wetted celite beads, which is practical method for cell immobilization. Repeated transfer of immobilized cells into fresh medium every 10 days increased productivity of immobilized cells and maximum concentration of lincomycin, 1007 $({\pm}256)$ mg/L, was obtained at the end of the ninth cycle. A 1.4-fold higher productivity was obtained in immobilized-cell culture than that obtained by suspended-cell culture. When pre-wetted beads were inoculated with vegetative mycelia and cultured a slightly higher amount of immobilized cells and lincomycin was obtained more than those obtained by culture of spores immobilized on dry beads. This result indicates that immobilization of mycelial cells on pre-wetted beads was readily available. This technique is simple and no additional facilities are required for cell immobilization.

A Study on Denture Satisfaction in Rural Elderly People (농촌지역 노인의 의치만족도)

  • Lee, Ga-Ryoung;Yoo, Wang-Keun
    • Journal of agricultural medicine and community health
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    • v.35 no.1
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    • pp.56-66
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    • 2010
  • Objectives: The study aimed to explore dental status and denture satisfaction in some rural elderly people. Methods: A total of 546 participants aged over 65 years was surveyed cross-sectionally. The subjects were surveyed with regard to their denture satisfaction by a structured questionnaire. The Wilcoxon or Kruskal-Wallis test was used for analysis. Results: As for respondents' satisfaction for dentures by the Likert scale of 5 score, aesthetic satisfaction (3.73) was highest, followed by general satisfaction (3.56) and masticatory functions satisfaction (3.45). In addition, the educational level, occupation, monthly income, the number of remaining teeth, use of denture variables have statistically significant difference in the denture satisfaction of those elderly people interviewed. Conclusions: The results showed that denture satisfaction in some rural elderly people was different in each categories. Consequently, providing tailor-made oral health education programs for the effective denture management of the elderly people should be needed in order to improve the quality of life of the aged.

Consideration about LINAC movable range by H&N patient immobilization device manufacture (두경부환자 고정기구제작을 통한치료기 가동범위에 관한 고찰)

  • Jung DoHyung;Shim JinSeop;Youm DuSeok;Choi GyeSuk
    • The Journal of Korean Society for Radiation Therapy
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    • v.16 no.2
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    • pp.63-67
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    • 2004
  • Purpose : New therapy technique appeared in 3D-CRT or IMRT according to a radiation treatment developing and worked. Such treatment technique requires the radiation irradiation of many direction. It has many restriction at radiation irradiation of many direction to the linear acceleration deception of now actually. Consequently We make new fix device and measure consequently the improvement of the activate range. Method and Material : We upload the fix device on a linear accelerator Couch. We fixed Gantry at 45, 90, 135 and Couch is spin and measure the clearance of the equipment. Couch is fixed at 0 45 90 and measures the clearance of Gantry. We upload the Extended head holder(EHH) on a linear accelerator Couch. and We measure with the experiment of the front. Result : The action range did not have big difference to increase Gantry45. but The activate range of Couch increases the angle in Gantry 90 and Gantry 135 when it uses EHH. The activate range of Gantry increases the angle in Couch 45 when it uses EHH. We showed good activate situation all in Couch 0 and Couch 90. The utility of EHH could keep a behind radiation diminution. Conclusion : The radiation irradiation of many direction comes to be possible the utility of the fix instrument(EHH). The safety space between the patient and equipment or between equipment and equipment increased the utility of the fix device. Also, The manufacture is possible imports to rather cheap price. and We could bring the frugality of the treatment expendable supplies.

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