• Title/Summary/Keyword: 계통육종

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Breeding of Fragrant Yellow Phalaenopsis and Scent Pattern Analysis by GC/SAW Electronic Nose System (유향성 황색계 호접란 육성 및 전자코를 이용한 향기패턴 분석)

  • Been, Chul-Gu
    • Horticultural Science & Technology
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    • v.28 no.4
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    • pp.656-663
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    • 2010
  • In order to improve the consumer acceptance of fragrant yellow hybrids of $Phalaenopsis$ ($Phal.$), crosses between yellow hybrid varieties were made and the obtained hybrids were investigated for horticultural characteristics as well as difference in fragrance patterns. Cross combination of $Phal.$ Brother lawrence and $Phal.$ Brother saragold yielded good seedling population of 500 plants. Segregation was noticed in color density, spot and stripe patterns on yellow color base of petal and also in fragrance. Six lines with multi-branch on flower stalk and strong fragrance flower were finally selected. Volatile fragrance components were compared among $Phal.$ Brother lawrence, $Phal.$ Brother saragold and their hybrids by GC/SAW electronic nose. In the derivative pattern of chromatogram and polar derivative pattern of fragrance, similar dominant peaks appeared on retention time 7-9 s and some hybrid lines had two strong peaks on retention time 20-25 s, respectively. Also in polar frequency pattern of fragrance obtained by $VaporPrint^{TM}$ image analysis among parent flowers and hybrids, an identical strong peak near 8 s of retention time was shown. This single fragrance component is considered a key element of fragrance in $Phal.$ Brother lawrence, $Phal.$ Brother saragold and their hybrids. This peak could be used as a marker for the breeding of fragrance in $Phalaenopsis$.

Development of an SNP set for marker-assisted breeding based on the genotyping-by-sequencing of elite inbred lines in watermelon (수박 엘리트 계통의 GBS를 통한 마커이용 육종용 SNP 마커 개발)

  • Lee, Junewoo;Son, Beunggu;Choi, Youngwhan;Kang, Jumsoon;Lee, Youngjae;Je, Byoung Il;Park, Younghoon
    • Journal of Plant Biotechnology
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    • v.45 no.3
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    • pp.242-249
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    • 2018
  • This study was conducted to develop an SNP set that can be useful for marker-assisted breeding (MAB) in watermelon (Citrullus. lanatus L) using Genotyping-by-sequencing (GBS) analysis of 20 commercial elite watermelon inbreds. The result of GBS showed that 77% of approximately 1.1 billion raw reads were mapped on the watermelon genome with an average mapping region of about 4,000 Kb, which indicated genome coverage of 2.3%. After the filtering process, a total of 2,670 SNPs with an average depth of 31.57 and the PIC (Polymorphic Information Content) value of 0.1~0.38 for 20 elite inbreds were obtained. Among those SNPs, 55 SNPs (5 SNPs per chromosome that are equally distributed on each chromosome) were selected. For the understanding genetic relationship of 20 elite inbreds, PCA (Principal Component Analysis) was carried out with 55 SNPs, which resulted in the classification of inbreds into 4 groups based on PC1 (52%) and PC2 (11%), thus causing differentiation between the inbreds. A similar classification pattern for PCA was observed from hierarchical clustering analysis. The SNP set developed in this study has the potential for application to cultivar identification, F1 seed purity test, and marker-assisted backcross (MABC) not only for 20 elite inbreds but also for diverse resources for watermelon breeding.

Chloroplast-targeted Expression of PsAPX1 Enhances Tolerance to Various Environmental Stresses in Transgenic Rice (엽록체에 과발현된 PsAPX1 유전자 형질전환 벼의 다양한 환경스트레스 내성 증진)

  • Park, Hyang-Mi;Kim, Yul-Ho;Choi, Man-Soo;Lee, Jae-Eun;Choi, In-Bea;Choi, Im-Soo;Shin, Dong-Bum;Lee, Jang-Yong;Kwak, Sang-Soo;Kwon, Suk-Yoon
    • Korean Journal of Breeding Science
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    • v.41 no.3
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    • pp.261-270
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    • 2009
  • Oxidative stress is a major damaging factor for plants exposed to environmental stresses. In order to develop transgenic rice plants with enhanced tolerance to various environmental stresses, PsAPX1, the gene of ascorbate peroxidase isolated from Pisum sativum was expressed in chloroplast under the control of an oxidative stress inducible sweet potato peroxidase2 (SWPA2) promoter (referred to as PsAPX1 plants). PsAPX1 transgenic plants showed enhanced tolerance to various environmental stresses, such as 170 mM NaCl, UV-B, ozone, 20% PEG, and drought in compared with non-transgenic (NT) plants. These results suggest that chloroplast-targeted over-expression of PsAPX1 gene could be very useful strategy for developing transgenic rice plants with increased tolerance to environmental stresses.

Morphometric Characterization of Honey Bee, Apis mellifera Linnaeus, Inbred Lines in Korea (국내 서양종꿀벌 순계의 형태적 특징)

  • Frunze, Olga;Сhoi, Yong Soo;Kim, Dong Won;Park, Bo Sun;Park, Hee Geun;Kang, Eun Jin
    • Korean journal of applied entomology
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    • v.59 no.4
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    • pp.371-382
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    • 2020
  • The A, C, F colonies of Apis mellifera ligustica Spin. and D, V colony of Apis mellifera caucasia Gorb. bees were collected from 2005-2007. Consequently, inbred lines were derived from the bees of original colonies by matting in the isolated island with due regard for pure breeding. This project helps in the selection of colonies with higher production capacity, aiming to improve honey and royal jelly production and breeding programs. Twenty-three standard morphological traits of honeybee were evaluated, and samples were compared with the data of the two original subspecies. The result suggested that 8 traits partly preserved in bees of inbred lines, and the bees from A. m. ligustica preserved more traits than bees from A. m. caucasia. Among the studied inbred lines, the F line is distinguished by an increase in leg parameters, considered as a favorable phenotypic trait of inbred lines. Importantly, bred of beelines in the same area can be classified as remote and isolated areas. Therefore, we observed differences of inbred lines with the origin subspecies in description acquired with morphometric characteristics as a result of adaptation, breeding, purebred individual lines used as an important resource for breeding novel cross-breeding colonies.

Analysis of Genetic Variation Among Popcorn Inbred Lines by SSR Markers (튀김용 옥수수 자식계통들에 대한 유전적 변이성)

  • Jang, Jin-Sun;Chang, Eun-Ha;Sa, Kyu-Jin;Kim, Jong-Hwa;Lee, Ju Kyong
    • Korean Journal of Breeding Science
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    • v.43 no.5
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    • pp.405-412
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    • 2011
  • Knowledge of genetic diversity and genetic relationships among inbred lines gives a significant impact on the selection of parental lines for hybrid maize varieties. Genetic diversity and genetic relationships among 86 popcorn inbred lines were analyzed using 50 SSR markers distributed over the whole genome. A total of 256 alleles were identified at all the SSR loci with an average of 5.1 and a range between two and sixteen per locus. The gene diversity values varied from 0.21 to 0.831 with an average of 0.579. The cluster tree generated using the described SSR markers recognized three major groups at 35.8% genetic similarity. Groups I, II, III respectively included 40, 39 and 7 inbred lines. The present study indicates that the SSR markers chosen for this analysis are effective for the assessment of genetic diversity and genetic relationships among 86 popcorn inbred lines in Korea.

Development of A New Phalaenopsis Cultivar 'Little Dew' with White Miniature Type Flowers (백색 미니 다화성계 호접란 신품종 '리틀 듀' 육성)

  • Been, Chul-Gu;Kim, Jin-Ki;Kim, Soo-Kyeong
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.2
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    • pp.149-151
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    • 2008
  • A new Phalaenopsis cultivar 'Little Dew' has been developed by a cross breeding and a line selection at the Flower Breeding Research Institute, Gyeongnam ARES from 2000 to 2007. Characteristics trials for this cultivar was carried out three times from 2004 to 2006. 'Little Dew' developed from a cross between Phal. 'Timothy Christopher' and Phal. amabilis showed white miniature type flowers and a multiflora shape. It has 3~4 flower stalks and many flowers, and long life span of flowers. This new cultivar was registered for commercialization in 2007 and would be cultured well in greenhouse conditions in Korea.

Screening for Soybean Pod Borer(Grapholitha glycinivorella Matsumura) Resistant Lines in Korean. Native Soybean Collections (재래종 대두의 콩나방 피해율에 관한 연구)

  • Kwon, S.H.;Ryu, J.;Kim, J.R.;Chung, K.H.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.22 no.2
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    • pp.93-97
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    • 1977
  • The rate of damaged soybean seeds by pod borer averaged 7.4% with the range of 1.2%-21.3% and tended to be higher in the lines with hairy pods comparing to those with hairless pods. The susceptibility of pod borer was negatively correlated with the pod setting date. while was not significantly affected by the weather conditions during the pod setting period.

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넙치의 우량혈통 선발육종 기술개발 II. 넙치 3계통의 혈액성분 분석

  • 방인철;박상용;이윤아;김종현
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2001.05a
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    • pp.385-386
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    • 2001
  • 넙치(Paralichthys olivaceus)는 1980년대 초부터 일본으로부터 수정란을 도입하여 양식되기 시작하였으며, 현재 우리 나라의 주요 해산 양식 대상종이다. 그러나 우리 나라에서 양식되고 있는 넙치는 대부분 일본산일 것으로 추정되고 있으며, 남해안의 경우 자연집단에 심각한 유전자 오염이 이루어져 남해안 고유계통은 찾아보기 어려운 실정이다(박 등, 1996a&b). (중략)

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Morphological Characteristics and Genetic Diversity Analysis of Cultivated Sancho (Zanthoxylum schinifolium) and Chopi (Zanthoxylum piperitum) in Korea (국내 재배지의 산초(Zanthoxylum schinifolium)와 초피(Zanthoxylum piperitum)의 형태학적 특성과 유전적 다양성)

  • Ryu, Jaihyunk;Choi, Hae-Sik;Lyu, Jae-il;Bae, Chang-Hyu
    • Korean Journal of Plant Resources
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    • v.29 no.5
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    • pp.555-563
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    • 2016
  • The morphological characteristics and genetic relationships among 32 germplasms of Zanthoxylum schinifolium and Zanthoxylum piperitum collected from two farms in Korea were investigated. The traits with the most variability were seed color, leaf size, and spine size. The intraspecific polymorphism of Z. schinifolium and Z. piperitum was 96.5% and 60.3%, respectively. The genetic diversity and Shannon’s information index values ranged from 0.11 to 0.33 and 0.19 to 0.50, with average values of 0.26 and 0.42, respectively. Two ISSR primers (UBC861 and UBC862) were able to distinguish the different species. The genetic similarity matrix (GSM) revealed variability among the accessions ranging from 0.116 to 0.816. The intraspecific GSM for Z. schinifolium and Z. piperitum was 0.177-0.780 and 0.250-0.816, respectively. The GSM findings indicate that Z. schinifolium and Z. piperitum accessions have high genetic diversity and possess germplasms qualifying as good genetic resources for cross breeding. The clustering analysis separated Z. schinifolium and Z. piperitum into independent groups, and all accessions could be classified into three categories. Z. Schinifolium var. nermis belonged to independent groups. Comparison of the clusters based on morphological analysis with those based on ISSR data resulted in an unclear pattern of division among the accessions. The study findings indicate that Z. schinifolium and Z. piperitum accessions have genetic diversity, and ISSR markers were useful for identifying Z. schinifolium and Z. piperitum.

A New Standard Rose Cultivar, 'Redme' with Red Color Petals (대륜계 적색 장미 신품종 '레드미' 육성)

  • Kim, Jin Ki;An, Dong Choon;Kim, Su Kycong;Been, Chul Gu;Park, Young Bae;Kim, Zhoo Hyeon
    • Korean Journal of Breeding Science
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    • v.40 no.3
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    • pp.348-351
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    • 2008
  • New bicolor standard rose (Rose hybrida Hort.) cultivars was developed in the Flower Research Institute, Gyeongnam ARES after going through seeding and line selection in 2000, and their characteristics were examined three times from 2003 to 2006, 'Redme' was crossed between 'Violina' and 'Akito' and it had a red Petals of a red-group (RHS No. 46A), few thorns and standard large flowers with a good harmony between ray and flower center Petals. The vase life of cut flower was 10 days. Registered as a commercial cultivars in 2006, the new cultivar can be planted in most greenhouse production region of Korea.