• Horticultural Science & Technology
• /
• v.19 no.4
• /
• pp.459-464
• /
• 2001

Leaf disks from cultivar 'Kennebec' and one selection line (ND 860-2) were cultured on Murashige-Skoog medium with various combinations of indole acetic acid (IAA) and zeatin riboside. Shoots, roots and callus were induced at various combinations of plant growth regulator levels. The medium containing $3.5mg{\cdot}L^{-1}$ IAA and $4.0mg{\cdot}L^{-1}$ zeatin riboside produced the most plantlets. Rooted regenerants were grown in the greenhouse. The growth of regenerated plants obtained from the MS medium supplemented with $7.0mg{\cdot}L^{-1}$ IAA and $3.0mg{\cdot}L^{-1}$ zeatin riboside was significantly greater than those grown from nodal expalnts. In ND 860-2, a leaf chimera with chlorophyll deficient (light yellow) sectors was found in plants regenerated fiom leaf disks (grown on MS medium supplemented with $3.5mg{\cdot}L^{-1}$ IAA and $3.0mg{\cdot}L^{-1}$ zeatin riboside) but not in plants grown from nodal explants. The phenotypic variability was also observed in the tuber number, size and weight.

• Journal of Plant Biotechnology
• /
• v.44 no.1
• /
• pp.89-96
• /
• 2017

The CRISPR/Cas9 is a core technology that can result in a paradigm for breeding new varieties. This study describes in detail the sgRNA design, vector construction, and the development of a transgenic plant and its molecular analysis, and demonstrates how gene editing technology through the CRISPR/Cas9 system can be applied easily and accurately. CRISPR/Cas9 facilitates targeted gene editing through RNA-guided DNA cleavage, followed by cellular DNA repair mechanisms that introduce sequence changes at the site of cleavage. It also allows the generation of heritable-targeted gene mutations and corrections. Here, we present detailed procedures involved in the CRISPR/Cas9 system to acquire faster, easier and more cost-efficient gene edited transgenic rice. The protocol described here establishes the strategies and steps for the selection of targets, design of sgRNA, vector construction, and analysis of the transgenic lines. The same principles can be used to customize the versatile CRISPR/Cas9 system, for application to other plant species.

• Horticultural Science & Technology
• /
• v.33 no.2
• /
• pp.298-301
• /
• 2015

A new Hibiscus syriacus variety, 'Dasom', was developed by radiation breeding using gamma ray exposure at the Korea Atomic Energy Research Institute (KAERI). Seeds of the original variety, 'Hongsun', were collected in 1993. These seeds were irradiated at 100 Gy dose and $M_1$ seeds were planted in a field of the KAERI in 1994. Lines with preferred flower color that were going to be used for street trees were initially selected in 1998. Analyses of characteristics were conducted from 2005 to 2010. The 5-year-old 'Dasom' plants were approximately 106 cm in height and averaged 19 branches, and thus were suitable for street trees. The flowers of 'Dasom' were semi-double type with medium red color and red eye spots. Petal length and width were about 4.0 cm and 3.3 cm, respectively. Flower diameter was slightly small, at about 5.8 cm, because of highly overlapping petals. The flowering period of 'Dasom' was about 105 days, which was 6 days shorter than that of the original variety, 'Hongsun'. However, the main charactersistics of 'Dasom' including leaf shape were similar to those of the original variety, 'Hongsun'.

• Journal of Embryo Transfer
• /
• v.22 no.3
• /
• pp.143-147
• /
• 2007

Records on 546 Korea native pigs for average daily gain (ADG), age at 70 kg (D70 kg) and backfat thickness (BF) made between 2001 and 2006 in herds on National Institutes of Animal Science in Korea were used to estimate genetic parameters. The data was analyzed by the DF-REML (Derivative-Free Restricted Maximum Likelihood) program of Boldman using a single-trait animal model. Heritabilities were 0.26, 0.09, and 0.29 for ADG, D70 kg and BF, respectively. The phenotypic correlations of ADG with D70 kg and BF were -0.71 and 0.30. The phenotypic correlation of D70 kg with BF was -0.15. The genetic correlations of ADG with D70 kg and BF were -0.11, 0.41, respectively. The genetic correlation of D70 kg with BF was -0.16. The data of weights and measurements on body length, body height and chest width after age at 11 months (days to 330) were shown scarcely less differences compare to data of age at 11 months.

• Journal of Plant Biotechnology
• /
• v.42 no.4
• /
• pp.336-341
• /
• 2015

Blueberry (Vaccinium spp.) is a bush that grows well at special cultural environments such as acid soil, high organic matter content, and a good drainage and aeration compared to other general crops. Blueberries are well known to contain high amounts of anthocyanins and phenolic compounds, resulting in high antioxidant activity that provides health benefits, and expanding the cultivation areas and consumer's demand in the worldwide. However, the full genome of blueberry has not been announced until now. Furthermore, the genomic analysis and transcriptome approaches are not so popular compare to major crops such as orange, apple, and grape. The aim of the review about blueberry genomic research is to establish the platform for setting blueberry breeding target, increasing proficiency of blueberry research, and making the practical cultivation techniques in Korea. The main topics in the blueberry genomic research including transcriptome, genetic mapping, and various markers are related with cold hardiness, chilling requirement, hot tolerance, anthocyanin content, and flavonoid synthesis pathway on various tissues like flower bud, leaf bud, shoot, root, and berry fruit. The review of the current status of blueberry genomic research will provide basic information to the breeders and researchers and will contribute to development of blueberry industry with sustainable productions and increase of blueberry consumption as new profitable crops in Korea.

• Journal of Plant Biotechnology
• /
• v.43 no.4
• /
• pp.466-472
• /
• 2016

This study analyzed the genetic diversity of 115 pear germplasms using 15 SSR markers. Three to forty-one SSR alleles were detected for each locus with an average of 16 alleles per locus. The average availability of markers was 0.966. The average observed heterozygosity ($H_{obs}$) was 0.603 (range: 0.140 to 0.929). The average expected heterozygosity ($H_{exp}$) was 0.718 (range: 0.463 to 0.904). The average polymorphism information content (PIC) was 0.692 (range: 0.403 to 0.897). The genetic relationships of pear germplasms were classified into two major groups by geographic origins and genetic characteristics according to genetic distance. The first group was composed of European pear belonging to Pyrus communis. The second group consisted of P. pyrifolia, P. ussuriensis, P. bretschneideri, P. betulaefolia, P. calleryana, interspecific hybrids, and unclear germplasms. The results of this study suggest that genotype analysis of pear germplasms using SSR markers can identify the genetic diversity of germplasms, and can be used to provide basic information for pear breeding.

• Journal of Mushroom
• /
• v.5 no.1
• /
• pp.34-38
• /
• 2007

The white-colored and the dark gray-colored mutants were frequently happened in cultivated areas of Pleurotus ostreatus (Wonhyeong-neutari). These caused conflicts between farmers and spawn companies. Our studies were conducted to elucidate the mechanism of mutagenesis. The results from the studies would provide valuable informations that could be used to prevent the color-related mutation, and also will be applied in breeding programs of P. ostreatus. Oyster mushroom variety, Wonhyeong-neutari, is somatic hybrid of Pleurotus and has genetic makers for arginine, ornithine, proline, riboflavine. Genetic markers analysis of monospore isolates derived from color mutants show identical tendency with that of Wonhyeong-neutari, these results indicate that color mutants were derived from Wonhyeongneutari. Twenty-one and four homokaryons were selected from the white-colored mutant MGL 2205 and gray-colored ASI 2029. All 34 F1 hybrids derived from the white-colored mutant MGL 2205 produce white-color fruiting bodies, indicating that the white color trait is heritable. In the first generation hybrids between the white-colored MGL 2205 and the gray-colored ASI 2029, all 16 hybrids produced pigmented fruiting bodies. Homokaryons isolated from the hybrid MGL 2205 X ASI 2029 were mated with homokaryon tester strains derived from MGL 2205. By these result, we could assumed that white color trait is a heritable character which is controlled by more than one recessive gene.

• Journal of Mushroom
• /
• v.15 no.4
• /
• pp.273-278
• /
• 2017

Hypsizygus marmoreus is a mushroom with abundant flavor and medicinal properties. However, its application is limited by problems such as long cultivation period, low biological efficiency, and microbiological contamination; therefore, there is a substantial need for development of new cultivars of this species. In this study, 55 strains of H. marmoreus were subjected to inter simple sequence repeat (ISSR) analysis to identify markers for the selection of mother strains for breeding from the collected germplasm. ISSR 13 and 15 were confirmed as polymorphic markers. The three strains (KMCC03106, KMCC03107, and KMCC03108) with white cap color were found to be genetically closely related upon UPGMA analysis of both ISSR 13 and 15. Based on the PCR analysis results for ISSR 15, the collected germplasm were differentiated into three groups according to the strain collection year. Thus, ISSR 15 could be a marker for determining the phylogeny of cap color and genetic variations according to the strain collection year. These results suggest that ISSR markers can be effective tools for the selection of mother strains for breeding of H. marmoreus.

• Horticultural Science & Technology
• /
• v.30 no.6
• /
• pp.776-779
• /
• 2012

A new cultivar 'Yeohong' was originated from an artificial cross between 'Jonathan' and 'Fuji' carried out at National Institute of Horticultural & Herbal Science in 1993. The cultivar was preliminarily selected among the elite siblings for its high fruit quality in 2002. After regional adaptability test at five districts for subsequent four years as 'Wonkyo Ga-31', it was finally selected in 2007. 'Yeohong' is characterized by the following. Optimum harvest time is late September. Shape of mature fruit at 278 g on mean weight is oblate a light red skin on a greenish yellow ground and yellowish white flesh. The fruit contains favorable total soluble solids at $14.3^{\circ}Brix$ and titratable acidity at 0.4%, which results in a gustatory harmony between sugars and acids. Safe storage period for the fruit is 3 weeks at room temperature. It is not resistant to Bitter rot. 'Yeohong' also reveals a physiological cross compatibility with leading cultivars such as 'Hongro' and 'Tsugaru', but a physiological cross incompatibility with 'Fuji'. It has weak growth habit.

• Horticultural Science & Technology
• /
• v.29 no.6
• /
• pp.655-658
• /
• 2011

A new cultivar 'Hongan' was originated from an artificial cross between 'Fuji' and 'Jonathan' carried out at National Institute of Horticultural & Herbal Science in 1993. The cultivar was preliminarily selected among the elite siblings for its high fruit quality in 2002. After regional adaptability test at five districts for subsequent four years as 'Wonkyo Ga-32', it was finally selected in 2006. 'Hongan' is characterized by the following. Optimum harvest time is late September. Shape of the mature fruit at 309 g on mean weight is globosity with a light red skin on a greenish yellow ground and yellowish white flesh. The fruit contains favorable total soluble solids at $13.7^{\circ}Brix$ and an acidity at 0.28%. Safe storage period for the fruit is 3 weeks at room temperature. It is resistant to Bitter rot. 'Hongan' also reveals a physiological cross compatibility with leading cultivars such as 'Gamhong' and 'Tsugaru' in addition to its maternal parent 'Fuji'. Tree topology is semi-spreading with vigorous growth habit.