• Horticultural Science & Technology
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• v.31 no.4
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• pp.517-521
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• 2013

A new cultivar Dendranthema grandiflourm 'Dream Round' was developed at Gyeonggi-do Agricultural Research & Extension Services (GARES), Korea in 2010. The variety 'Dream Round' was initially derived from a cross in 2006 between 'Hebo' a seed parent, a spray chrysanthemum cultivar with white anemone type, and 'Samos' as a pollen parent, a spray chrysanthemum variety with white anemone type. The cultivar has anemone type with white petals. Trial evaluation was conducted from 2008 to 2010 for the selection of that cultivar, including a shading culture in spring and a retarding culture in summer. The flowering time of 'Dream Round' was October 24th, and year-round flowering was possible by shading or lighting treatment. The diameter of flower of 'Dream Round' was 34.1 mm. Numbers of flowers per stem and petals per flower of 'Dream Round' were 12.8 and 26.1, respectively. Its leaf color was green (Green Group 147A) and plant height was 92.4 cm. Days to flowering of 'Dream Round' under the short day treatment was about 54 in spring, and diameter of flower of 'Dream Round' was 34.2 mm in the summer. The vase life of 'Dream Round' was 21.7 days in autumn.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.715-718
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• 2010

$Cymbidium$ 'Purple Princess' ('Show Girl' x 'Eiko') is a new cultivar having dark purple sepal and petal (RHS, RP59A) with purple lip (RHS, RP59A). A cross was made between pink colored $C.$'Show Girl' as maternal line and dark purple colored $C.$ 'Eiko' as paternal line in 1994. It was preliminarily selected as Line No. 94019267 in 1999 considering the characters of flower shape and color, leaf growing shape and growth habit in normal culture condition. Multiplication, cultivation, and characteristic trials were conducted from 2000 to 2003. The line was named as Wongyo F1-7 and phenotype was characterized in 2004 as a new 'Purple Princess'. 'Purple Princess' has about 13.2 flowers per flower stalk and flower size of 5.2 cm. General appearance of petals and sepals is slightly incurved shape. The plant size is intermediate having erect peduncle. Blooming starts from early December under optimal culture condition. Leaf attitude and twisting is erect and very weak respectively. Erect leaf will be suitable for growing and handling in the nursery as pot orchid flowers.

• Journal of Korean Society of Forest Science
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• v.102 no.2
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• pp.198-203
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• 2013

Herein we analyzed the nuclear DNA content and the histological characteristics of the triploid of the 'Hyunsasi' (Populus alba ${\times}$ P. glandulosa $F_1$) which were developed for biomass production and molecular breeding research. The flow cytometric analysis showed that the nuclear DNA content of the 3 triploids were 1.6 times greater than those of the diploid. In terms of histological characteristics, the cross-section area of the stem of 'Line-18' was 1.6 times larger than that of the diploid. The area of pith, and cortex and phloem of the stem of 'Line-18' was also 1.6 and 2.0 times larger than that of the diploid, respectively. Moreover, the length and area of guard cell of 'Line-18' was 1.2 times larger than that of the diploid. These results helps to understand the cytological characteristics of the triploid poplar clones, and further investigations in the growth rate and wood properties of the triploids growing in the field will determine whether the triploid poplars are good candidates for molecular breeding programs and for the improvement of industrial biomass productivity.

• Korean Journal of Plant Resources
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• v.21 no.5
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• pp.388-394
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• 2008

This study was carried out to develop new cultivars using the $T_5$ generation of transformed rice by PCR analysis with DNA marker in each generation $(from\;T_3\;to\;T_5)$. In the previous study, we successfully developed the transgenic rice plants over-expressing the Arabidopsis $H^+/Ca^{2+}$ antiporter CAX 1 (accession no. U57411) gene. The calcium concentration in brown rice of transgenic plants was higher than that of donor plants, Iipum, and was selected 3 lines out of 25 lines at cultured GMO field. The major agronomic traits such as culm length, panicle length and panicle number of 3 lines at transgenic plants $(T_5)$ were similar to wild type. Also these lines appeared to have disease resistance to rice blast, cold resistance as compared with donor types. The grain shape was similar to donor plant, however, the 1000 grain weight of brown rice was different from transgenic plants. These finding would be used for basic data of new variety registration.

• Horticultural Science & Technology
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• v.28 no.3
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• pp.519-521
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• 2010

An Asiatic lily cultivar 'Asian Flame' was bred in 2007 at National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA) of Korea. The crossing was conducted between female parent A94-34 ('Montreux' ${\times}$ 'Anabelle') and male parent 'Fire' by stigmatic pollination at Suwon in 1996. First selection was done and tentatively named as A98-98 in 1998. After in vitro multiplication and bulb production of A98-98 line, growth and flowering characteristic tests has been conducted from 2005 to 2007. The evaluation of characteristics and preference were surveyed at a lily flower show of NIHHS in 2007 in Taean. The new cultivar 'Asian Flame' flowers in middle of June and grows to an average of 122.7 cm in height. Flowers bloom upward-facing, red (RHS 45B) petals. Year-round flowering is possible by storage of the bulb under $-1.5^{\circ}C$ conditions. For forcing culture, it is necessary to add calcium to the fertilizer or remove side scales of large bulb to prevent leaf scorch.

• FLOWER RESEARCH JOURNAL
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• v.17 no.1
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• pp.40-43
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• 2009

'Glory Pink' an interspecific lily cultivar was released in 2007 at National Horticultural Research Institute (NHRI), Rural Development Administration (RDA), Suwon, Korea. The cross and ovule culture was made between FA interspecific hybrid 'FA97-30' (L. formolongi 'Raizan' ${\times}$ Asiatic lily 'A61'), a red colored cultivar, and Asiatic lily 'Sanzio', light red colored cultivar in 2001. Multiplication and bulbing, and characteristic tests were conducted from 2005 to 2006. Flowering time is around the first of July and the plant height is 131.7 cm. Flowers are upward-facing and red-pink (RHS 63C), with size of 11.3 cm. Petal length and width is 9.1 cm and 4.1 cm, respectively. Leaves are 13.8 cm long, 1.4 cm wide. The throat is dark green, and the stigma is light yellow and pollen is light brown. The weight and size of bulb is 51.1 g and 13.2 cm, respectively. For long-term storage, bulbs can be stored under -1 to -2 for year-round forcing.

• FLOWER RESEARCH JOURNAL
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• v.19 no.4
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• pp.231-237
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• 2011

In this study, we performed ovule culture after reciprocal crosses of two Alstroemeria accessions and investigated genetic contribution of parents by using RAPD markers. The best method was half-ovule culture on MS medium supplemented with $60g{\cdot}L^{-1}$ sucrose and $2.2g{\cdot}L^{-1}$ gelrite at 14 days after pollination. Embryos began to germinate after 6 weeks of culture. The complete plantlets were formed after 4 months of culture. In eight progenies and two parental cultivars, 59 polymorphic bands were obtained out of 89 total bands by RAPD analysis using 7 primers. Eight $F_1$ progenies from the crosses between two accessions using reciprocal crosses showed 1:1 contribution of maternal and paternal parents. It is confirmed that $F_1$ progenies were obtained from parental accessions by using RAPD markers. We conclude this cross combination showed pre-fertilization barriers with incompatibility between stigma or style, and pollen because progeny number was different in each cross combination. Thereby, it warrants overcoming pre-fertilization barrier together with post-fertilization barrier in order to broaden the heterozygosity within progeny populations in Alstroemeria breeding program.

• FLOWER RESEARCH JOURNAL
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• v.17 no.1
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• pp.36-39
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• 2009

An Asiatic lily cultivar 'Apricot King' was bred in 2007 at National Horticultural Research Institute (NHRI), Rural Development Administration (RDA), Suwon, Korea. The cross was made in 2002 between Asiatic lily 'Orlando', a light orange colored cultivar, and 'Corrida', yellow and pink colored cultivar. Preliminarily selection was done as 'A04-72' in 2004. Multiplication and bulb formation, and characteristic tests were conducted from 2005 to 2006. The evaluation of this line was performed and named as 'Wongyo C1-84' in 2007 that was registered as 'Apricot King' to the registration office of Korea Seed & Variety Service. 'Apricot King' flowers at the end of June and grows average 121.6 cm. It flowers upward-facing, dark green throat and yellow orange (RHS 20A) with the size of flower 17.9 cm. Mean petal length and width is 12.8 cm and 3.7 cm, respectively. Leaves are 16.1 cm long and 2.1 cm wide. It shows light orange stigma and pollen. The weight and size of bulb is 64.2 g and 17.6 cm, respectively. For long-term storage, bulbs can be stored under -1 to $-2^{\circ}C$ for year-round forcing.

• Korean Journal of Plant Resources
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• v.17 no.3
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• pp.304-313
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• 2004

This study was conducted to find out the scientific maize breeding protocol for developing high performing single cross hybrid using introduced U.S. elite inbred lines; the pattern of inheritance, heterosis and heritabilities of six agronomic traits were studied in the progenies derived from five crosses (Mo17/B14A, Va85/B73, C103/ND203, FR35/Oh43, Wf9/A632). Among the five cross combinations, the cross combination of Mo17/B14A showed the highest heterosis for the most agronomic traits. Among 6 agronomic traits, the grain yield showed the highest heterosis effect in most cross combinations. Most of the agronomic traits in this study showed more than 50% heritability for six cross combinations, with an exception of the ear length trait. In conclusion, since Mo17/B14A showed excellent performance for most of the agronomic traits, these inbred lines were desirable combination and regarded as superior germ plasm sources for F1 hybrid development. The results of current studies will be utilized for developing high performing single cross hybrid from maize inbred lines, and will be used for the further genetic analysis of agronomic traits and maize breeding programs.

• Journal of Life Science
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• v.29 no.7
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• pp.774-778
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• 2019

Soybean [Glycine max (L.) Merr.] is one of the major food sources of protein, oil, carbohydrates, isoflavones, and other nutrients for both humans and animals. However, soybean seeds contain antinutritional factors, such as lectin protein, Kunitz Trypsin Inhibitor (KTI) protein, and stachyose. The objective of this research was to stack recessive alleles for development a triple recessive genotype, titilelers2rs2, with low KTI protein, lectin protein, and stachyose contents. Three parents (Gaechuck#2, PI200508, and 14G20) were used to develop the breeding population. The presence or absence of the lectin and KTI proteins was detected by western blotting. The stachyose content in mature seeds was determined by HPLC. Agronomic traits, such as plant type, plant height, maturity date, lodging, seed quality, and 100-seed weight, were evaluated for the four $F_3$ plant strains. One $F_4$ plant strain with the desired agronomical traits was selected. One new strain with the triple recessive titilelers2rs2 genotype was developed. The plant height of the new strain was 51 cm and the 100-seed weight was 31.0 g. The new strain had a yellow seed coat and yellow hilum. The stachyose content of the new strain was 3.8 g/kg. One strain developed in this research will be used to produce improved yellow soybean cultivars that are free of lectin and KTI proteins and low in stachyose content.