• Korean Journal of Breeding Science
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• 제50권4호
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• pp.516-521
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• 2018

"Nulichal" was developed by the National Institute of Crop Science, RDA, in 2010. The initial cross was conducted in 2000, and an elite line (HB15305-B-B-31-2) was selected in 2005. Subsequently, preliminary yield and advanced yield trials have designated it as "Iksan 95." It showed good agronomic performance in regional yield trials from 2008 to 2010 and was released as "Nulichal," with resistance against the barley yellow mosaic virus (BaYMV) and high yields with a waxy endosperm. The average heading and maturation dates of "Nulichal" were April 24 and May 30, respectively in paddy fields, which were 1 day later than those of the check cultivar "Saechalssalbori." It had culm and spike lengths of 82 and 4.0 cm, respectively. It showed $774spikes/m^2$, 63 grains/spike, 25.0 g of 1,000-grain weight, and 793 g of test weight. "Nulichal" showed higher levels of resistance to BaYMV than the naturally occurring Iksan (type III strain), Naju (type I), and Jinju (type II). The expansion rate was higher than that of "Saechalssalbori" and its hardness, gumminess, and chewiness were lower than those of "Saechalssalbori." Its average pearled grain yield in the regional yield trial was 4.00 MT/ha in paddy fields, which was 10% higher than that of the check cultivar. The combined availability of "Nulichal" was improved by its increased culm length compared with that of "Hinchalssal."

• KOREAN JOURNAL OF CROP SCIENCE
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• 제58권2호
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• pp.196-202
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• 2013

This study was conducted to evaluate the performances of rice transgenic lines along with their wild types in terms of agronomic traits. A total of 32 rice transgenic lines, through previously conducted collaborative researches between molecular biologists and conventional rice breeders, were selected as promising lines. As the introduced functional genes, 17 genes, which were putatively related with high yield, disease and herbicide resistance, abiotic stress tolerance, and diversifying endosperm starch components, were transformed into three Japonica cultivars, Nipponbare, Nagdongbyeo, and Dongjinbyeo. The transgenic lines exhibited significantly deviated performances from their wild types on agronomic traits such as days to heading, culm length and yield potential. Multivariate analyses on transgenic lines to the evaluated agronomic traits also indicated random manner of phenotypic deviations from their wild type in terms of deviation directions and degrees. Our results suggested that, therefore, breeding strategies to control unexpected deleterious phenotypic performances among transgenic lines would be critical as much as the functions and proper expressions of the transformed genes.

• KOREAN JOURNAL OF CROP SCIENCE
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• 제50권spc1호
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• pp.112-117
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• 2005

This study was conducted to see the feasibility of breeding for sprout soybean cultivar with minimum benny flavor using lipoxygenase-less lines. Lipoxygenase-less cultivar Jinpumkong2 was crossed by lipoxygenase containing Gwangankong, Sebaeknamulkong, and Pureunkong as paternal parent and 24 lipoxygenase-less lines derived from those 3 combinations were selected and those lines were evaluated with their parental cultivars. Germination rate showed no difference between lipoxygenase-less lines and their parental cultivars, however, rates of normal sprout of those and Jinpumkong2 were 63 and $56\%$, and were lower than that of paternal parents. Hypocotyl length of those was same as Jinpumkong2, however, shorter than paternal parents. Texture characteristics including hardness, cutting force and mastication of 96 hour-cultured sprout of lipoxygenase-less lines showed higher value than that of their parental cultivars. Lipoxtgenase isozyme was not detected in the sprout cotyledon of lipoxygenase-less lines, however it was observed in the sprout hypocotyl of all the used genotypes. Though lipoxygenase activity in the seed of lipoxygenase-less lines was lower than that of Jinpumkong2(0.477, ${Delta}A$ 234 nm min-1 mg meal-1),2 lines revealed more than 0.5 value. Lipoxygenase-activity of 2 day-cultured sprout(both cotyledon and hypocotyl) was the highest, decreased in 3 days after culture and re-increased thereafter. Several lipoxygenase-less lines with lower lipoxygenase activity of sprout than Jinpumkong2 were selected and this suggested the possibility of breeding lines for soy-sprout with low benny flavor.

• Journal of Sericultural and Entomological Science
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• 제46권1호
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• pp.1-5
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• 2004

Rutin has many beneficial effects on human health. It was established that rutin antagonizes the increase of capillary fragility associated with hemorrhagic disease, reduces high blood pressure, decreases the permeability of the vessels, and has an antiedema effect, reduces the risk of arteriosclerosis, and shows antioxidant activity. Rutin analysis with mulberry fruits was carried out by high-performance liquid chromatography (HPLC) to reveal the possibility of source of functional food. As a result, mean content of fifty accessions was 0.14±0.050% DW. Among the tested fifty accessions, 'Sabangso' was showed the highest rutin in 0.29%DW, whereas 'Simseol' was the lowest content in 0.05% DW. Also, we researched the change of rutin content according to harvested date. The rutin contents of earlier harvested groups were higher than later harvested groups. Simultaneously, fruity characteristics as well as rutin content were researched and analyzed to select the functional mulberry varieties for the production of fruit. From the six accessions which were contained high rutin content, we selected three suitable varieties such as 'Ficus', 'Kangsun', and 'Palcheongsipyung'.

• Journal of Sericultural and Entomological Science
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• 제52권2호
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• pp.129-133
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• 2014

We analyzed rutin content using mulberry genetic resources. They were grown under the same environment and conditions. Mulberry leaves were collected and then freeze-dried and powdered for rutin test. As a result, mean content of mulberry strains was $0.38{\pm}0.17%$, and the coefficient of variation (CV) was 45.7%. The variation was greatly severe. Among the tested strains, 'Jangloe' was showed the highest content of 0.97%, whereas 'Sungsu 3' was showed the lowest content of 0.01% respectively. Finally we selected rutin high-containing 6 strains. They are as follows. 'Jangloe', 'Pumbo 6', 'Youjin', 'Kaeryangjeonjeon', 'Baekhak', and 'Pumbo 32' which are more than twice of the overall average content.

• Journal of Apiculture
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• 제32권3호
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• pp.147-154
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• 2017

Honeybees (Apis mellifera) are common pollinators and important insects studied in agriculture, ecology and basic research. Recently, RDA (Rural Development Administration) and YIRI (Yecheon-gun Industrial Insect Research Institute) have been breeding a triple crossbred honey bee named Jangwon, which have the ability to produce superior quality honey. In this study, we identified a single nucleotide polymorphism (SNP) marker in the genome of Jangwon honeybee, particularly, in the paternal line (D line). Initially, we performed Sequence-Based Genotyping (SBG) using the Illumina Hiseq 2500 in 5 honeybee inbred lines; A, C, D, E, and F; and obtained 1,029 SNPs. Seventeen SNPs for each inbred line were generated and selected after further filtering of the SNP dataset. The 17 SNP markers validated by performing TaqMan probe-based real-time PCR and genotyping analysis was conducted. Genotyping analysis of the 5 honeybee inbred lines and one hybrid line, $D{\times}F$, revealed that one set of SNP marker, AmD9, precisely discriminated the inbred line D from the others. Our results suggest that the identified SNP marker, AmD9, is successful in distinguishing the inbred honeybee lines D, and can be directly used for genotyping and breeding applications.

• Proceedings of the Plant Resources Society of Korea Conference
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.52-52
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• 2020

유채(Brassica napus L.)는 식용유 생산을 목적으로 재배되며, 1975년에는 26.8천ha가 재배되어 연간 생산량이 34.7천톤이 생산되었으나, 외국으로부터 값싼 식용유의 대량수입으로 재배면적이 급격하게 감소되었다. 그러나 최근에 지방자치단체를 중심으로 경관용 재배면적이 증가하고 있고, 건강에 대한 관심이 높아짐에 따라 국내산 non-GM 식용유 생산을 목적으로 유채 재배면적이 급격하게 증가하고 있다. 유채의 대량생산을 위해서는 논 재배가 필수적이나 유채와 벼의 이모작 재배 시기가 겹치는 문제점이 발생하고 있어 조생종 유채의 품종개발과 함께 봄 파종이 가능한 유채의 품종 육성에 대한 요구가 매우 높다. 따라서 본 연구에서는 국내 보유 유채를 포함하는 십자화과(Cruciferae) 유전자원의 특성 평가를 통해 극조숙 유채 품종 육성을 조기에 육성하기 위해 실시하였다. 농촌진흥청 국립유전자원센터에서 분양받은 유전자원 146계통을 파종 재배하면서 핵형분석과 외부형질을 기준으로 정확한 종을 동정하였고, 각 유전자원의 생육 및 개화특성을 조사하였다. 유전자원들을 세포분석기를 이용하여 핵형을 분석한 결과, 전체 146개의 유전자원 중 유채(B. napus L.) 128계통, 갓(B. juncea Sinsk) 6계통, 배추(B. campestris Makino) 9계통, 미분류 3계통 등으로 조사되었다. 유전자원을 가을 파종(10월 22일)하였을 때 개화가 가장 빠른 계통(IT 279089)이 파종 후 137일 이후인 3월7일부터 개화가 시작되었고, 가장 늦은 계통(IT 279198)은 191일 후인 4월 30일에 개화가 시작되었다. 봄 파종(2월 4일)하였을 때 개화가 가장 빠른 계통(IT 279089)이 파종 후 79일 이후인 4월 23일부터 개화가 시작되었고 IT 279092 등 29계통은 추대와 개화가 되지 않았다. 위와 같은 연구결과를 토대로 개화시기가 빠른 유전자원을 육종을 위한 인공교배 시화분친이나 종자친으로 이용하면 지방산 품질이 좋고 극조숙성인 유채 품종의 개발이 가능할 것으로 생각된다.

• Current Research on Agriculture and Life Sciences
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• 제18권
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• pp.9-17
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• 2000

In an effort to breed lines resistant to both bacterial wilt and Phytophthora blight, F5 and BC1F4, and F6 and BC1F5 generations of the crosses between the lines previously bred for resistance to Phytophthora blight ((16-2-2-3-2 = Phytophthora-resistant 'Kalmi', 19-1-3-7-1-1, 19-2-4-5-3-2 = Phytophthora-resistant 'Subi', Kim et al., 1996) and sources of resistance to bacterial wilt (KC350 = MC-4 or KC353 = PBC631, Kim et al., 1998) were evaluated for resistance to both bacterial wilt and to Phytophthora blight in 1999 and in 2000, respectively. Plants which were highly resistant to both bacterial wilt and Phytophthora blight were selected for development of the next generations.

• Journal of Life Science
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• 제18권7호
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• pp.907-911
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• 2008

Radish (Raphanus sativus L.) is one of very important crop plants in the world. It is very important to determine hybrid seed quality in the production of hybrid Brassica vegetable seeds to avoid unacceptable contamination with self-inbred (sib) seeds. The allozyme for evaluating seed purity in a commercial $F_1-hybrid$ radish cultivar is demonstrated. Three hundred sixty seeds from the male and female harvest were subsequently screened for seed purity using 27 isozyme loci. Especially, F1 hybrids of radish, Per-1 ($aa{\times}bb$), Lap-1 ($aa{\times}bb$), Est-1 ($aa{\times}bb$) were presented clear hybrid bands. Est-1 locus revealed that 15 (8.3%) seeds from the female harvest and 26 (14.4%) seeds from the male harvest were sibs. It maintains higher than average level of genetic diversity compared with their correspondent parents. Shannon's index of phenotypic diversity (I) of hybrids was the highest of all accessions (R. sativus L. cv. Daepeng, R. sativus L. cv. Backza, and their hybrids). The allozyme may lead to a better insight into the hybrid seed purity.

• Journal of Plant Biotechnology
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• 제43권2호
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• pp.164-173
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• 2016

To investigate the genetic basis of phenotypic differences, sequence variations were analyzed in 8 inbred watermelon lines by re-sequencing. The number of sequence variations differed depending on the chromosome. Only 12.9% of SNPs were found within genes, whereas the rest were detected in promoter or intergenic regions. SNP density analysis showed that there was a highly variable region at the end of chromosome 6, which is similar to previously published findings. However, this region with high SNP density did not show much variation between the lines. In contrast, highly conserved regions with a size of 6.5-10 Mb were found in chromosomes 10 and 11. Pathway analysis suggested that the DIMBOA (a natural antibiotic)-glucoside degradation pathway was significantly different between the lines, indicating that the eight lines may have different levels of pathogen resistance. Among the carbohydrate-related genes, the alpha-galactosidase gene was the most variable among the lines. Information from this study will be helpful in understanding the watermelon breeding process at the molecular level.