• Title/Summary/Keyword: 계측현미경

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Characterization of Soot Particles Generated in Non-sooting and Sooting Normal Diffusion Flames (Sooting 및 Non-Sooting 정상 확산 화염에서 생성되는 매연 입자의 특성에 대한 연구)

  • Choi, In-Chul;Lee, Jae-Bok;Hwang, Jung-Ho
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.24 no.7
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    • pp.984-993
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    • 2000
  • Characteristics of carbon soot particles generated in diffusion flames were studied. Non-sooting and sooting normal diffusion flames using propane or ethylene as fuel were selected. In the flames, soot volume fraction was measured by a thermocouple, and primary particle diameter and cluster size were analyzed by TEM photographs. The characteristics of soot particles depended on flame(non-sooting or sooting) and fuel(propane or ethylene) type. Unlike the sooting diffusion flames, particle growth and oxidation processes were clearly observed in the non-sooting diffusion flames. In the sooting diffusion flames, soot particle size was slightly changed at the flame tip.

Study on Three-dimensional Visualization of Cell Nuclei: Volume Rendering and Surface Rendering (세포핵의 3차원 가시화 방법에 관한 연구 : 볼륨 랜더링과 표면 렌더링)

  • 김태윤;천창호;최현주;최익환;최흥국
    • Proceedings of the Korea Multimedia Society Conference
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    • 2004.05a
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    • pp.183-186
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    • 2004
  • 2차원 영상 기반의 세포 분석은 2차원 평면 상에서의 관찰만 가능하므로 정확하고 객관적인 분석에 한계가 있다. 따라서 이러한 한계를 극복하고자 컨포컬 현미경을 통해 획득한 연속적인 2차원 단면 영상들로 구성된 볼륨 데이터를 볼륨 및 표면 렌더링을 통해 가시화 하여, 세포핵에 대한 다양한 각도에서의 형태 관찰이 가능하도록 하였다. 또한 세포핵 볼륨에서 ROI 추출을 통해 국부 영역 분석의 효율성을 높였다. 그리고 이 결과를 바탕으로 정확한 암 세포의 계측 및 정량적 분석을 통해 이후 자궁암 환자에게 최적의 진단 및 지료를 제공할 수 있는 연구 기반을 마련하고자 한다.

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EFFECT OF ETCHING TIME ON ENAMEL SURFACE ROUGHNESS: CONFOCAL LASER SCANNING MICROSCOPIC STUDY (공초점 레이저주사현미경을 이용한 산부식 시간에 따른 법랑질 표면 양상에 관한 연구)

  • Kam, Dong-Hoon;Kim, Jung-Wook;Jang, Ki-Taeg;Lee, Sang-Hoon;Kim, Chong-Chul;Hahn, Se-Hyun
    • Journal of the korean academy of Pediatric Dentistry
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    • v.30 no.1
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    • pp.41-46
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    • 2003
  • In order to evaluate the sufficient etching time for successful bonding and also minimizing unnecessary mineral loss, the enamel surface roughness analysis was performed using confocal laser scanning microscopy. Sixty extracted sound human molar teeth were imbedded in the center of acrylic cylinder using self-curing clear resin exposing buccal surface, and then polished with series of SiC paper(220, 500, 800, 1000, 2000, 4000 grit). Each specimen was randomly assigned to six groups(N=10). 37% phosphoric acid was applied to the polished tooth surface for 10, 20, 30, 40, 50, 60 seconds respectively and washed with copious water. After the surface roughness analysis, five roughness parameters(Sa, Sq, Sz, Sdr, Ra) were statistically analysed by ANOVA and Duncan post hoc test. We found that the all five parameters had higher roughness value in 30 seconds etching time, especially parameter Sz showed the lowest value in 10 seconds etching time and the highest value in 30 seconds etching time compared with the other etching times(p<0.05).

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Surface Crack Evaluation Method in Concrete Structures (콘크리트 구조물의 표면 균열 평가 기법)

  • Lee, Bang-Yeon;Yi, Seong-Tae;Kim, Jin-Keun
    • Journal of the Korean Society for Nondestructive Testing
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    • v.27 no.2
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    • pp.173-182
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    • 2007
  • Cracks in concrete structures should be measured to periodically assess potential problems in durability and serviceability. Conventional crack measurement systems depend on visual inspections and manual measurements of the crack features such as width, length, and direction using microscope and crack gage. However, conventional methods take long time as well as manpower, and lack quantitative objectivity resulted by inspectors. In this study, an evaluation technique for concrete surface cracks is developed using image processing and artificial neural network. Developed technique consists of three major parts: (1) crack detection (2) crack analysis and (3) pattern recognition. To examine validity of the technique developed in this study, crack analyzing tests were performed on the images obtained from various types of concrete surface cracks. The test results revealed that the system is highly effective in automatically analyzing concrete surface cracks in terms of features and patterns of cracks.

A Preliminary Study on Structure of the Wooden Printing Blocks in Japan - Based on the 3D Measurement Method - (일본 판목의 구조에 대한 기초연구 - 3D 계측을 통한 조사를 중심으로 -)

  • Ando, Mariko;Ryu, Sungwook;Imazu, Setsuo
    • Journal of Conservation Science
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    • v.33 no.1
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    • pp.11-16
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    • 2017
  • This study reviews the structure of wooden printing blocks in Japan, focusing on the blocks as three-dimensional objects. Inspection is more effective three-dimensionally than two-dimensionally, and for the first time in wooden printing block research, the study uses a 3D CT scanner and a high-resolution 3D digitizer. The 3D CT scanner examines cross sections of the blocks and identifies their grain and contents, including insects surviving within them. The 3D digitizer enables observation of objects up to 0.02 mm; this allows detailed collection of block surface information, which is difficult to identify with a conventional microscope.

The Morphology and Morphometry of the Olfactory Organ of Southern King Spine Loach, Iksookimia hugowolfeldi (Cypriniformes, Cobitidae) (한국고유종 남방종개 Iksookimia hugowolfeldi 후각기관의 형태 및 형태계측학적 연구)

  • Kim, Hyun Tae;Park, Jong Young
    • Korean Journal of Ichthyology
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    • v.32 no.2
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    • pp.49-54
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    • 2020
  • The morphometry and morphology of the olfactory organ of the southern king spine loach Iksookimia hugowolfeldi were investigated by stereo microscopy and statistical analysis. Its external morphology consists of two holes, the anterior and posterior nostrils. The anterior nostril (0.35~0.53 mm in diameter) forms tubular process protruding vertically from each side of the snout. The posterior nostril (0.32~0.68 mm in major diameter) is a eye-like hole parallel to the skin. Internally, the rosette structure is set with 14 to 21 lamellae and medium raphe on the inner floor. In morphometry, its lamellar number (male vs. female; 18±1.8 vs. 17±1.6, P<0.05) and lamellar number to standard length ratio (24.2±1.3% vs. 21.7±2.5%, P<0.05) was larger in male than female. The lamellar number and standard length have high correlation in male (P<0.001) but not in female (P=0.170). It seems that such characteristics of the olfactory organ of I. hugowolfeldi are related to its bottom-dwelling life which digs and takes a rest under sand and sexual dimorphism by a distinct degree in olfactory dependence for reproduction of each sex.

Experience of Reticulocytes Measurement at 720 nm Using Spectrophotometer (분광광도계를 이용한 720 nm에서 망상적혈구 측정 경험)

  • Sung, Hyun-Ho;Seok, Dong-In;Jung, You-Hyun;Kim, Dae-Jung;Lee, Seok-Jae
    • Korean Journal of Clinical Laboratory Science
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    • v.49 no.4
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    • pp.382-389
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    • 2017
  • Currently, reticulocyte experimental calculation technology used in clinical laboratories are divided two types: manual and automated. Manual reticulocyte counting using a microscopy lacks accuracy due particularly to its low reproducibility, affecting the accuracy of manual reticulocyte count. Moreover, Automatic blood corpuscle analyzer flow cytometry is difficult to be used in underdeveloped countries and small scale laboratories due to relatively high cost. Therefore, this study tried to find a new method to complement these drawbacks. The aim of this study was to compare the stained reticulocytes count by spectrophotometer and also to analyze the statistics of spectrophotometer and flow cytometer. The same 8 EDTA samples were repeated 36 times to compare the agreement between spectrophotometer and flow cytometer. This study measured the specimen diluted 600 times at 700~780 nm by 10 differences. Wavelength between 710 to 730 by absorbance showed a positive correlation between standard data and test data (r=0.967, p<0.01), presenting a correlation between variables. Statistical analyses of regression for test and standard parametric data, the optimal dilution factor was 600 times. Therefore, this study tried to technical utilizes such as contributing economical for the reticulocyte absorbance apply from the auto spectrophotometer, a monitoring system for the reticulocyte relation anemia, etc. Therefore, more extensive studies, including an auto chemical analyzer application, will be needed.

Development of an AFM-Based System for Nano In-Process Measurement of Defects on Machined Surfaces (가공면미세결함의 나노 인프로세스 측정을 위한 AFM시스템의 개발)

  • Gwon, Hyeon-Gyu;Choe, Seong-Dae;Park, Mu-Hun
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.26 no.3
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    • pp.537-543
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    • 2002
  • This paper examines a new in-process measurement system for the measurement of micro-defects on the surfaces of brittle materials by using the AFM (Atomic Force Microscopy). A new AFM scanning stage that can also perform nano-scale bending of the sample was developed by adding a bending unit to a commercially available AFM scanner. The bending unit consists of a PZT actuator and sample holder, and can perform static and cyclic three-point bending. The true bending displacement of the bending unit is approximately 1.8mm when 80 volts are applied to the PZT actuator. The frequency response of the bending unit and the stress on the sample were also analyzed, both theoretically and experimentally. Potential surface defects of the sample were successfully detected by this measurement system. It was confirmed that the number of micro-defects on a scratched surface increases when the surface is subjected to a cyclic bending load.

An Investigation on the Macroscopic Spray Behavior of Nonimpinging-type Injector through Optical Measurement Technique (광학계측기법에 의한 비충돌형 인젝터의 거시적 분무거동 고찰)

  • Kim, Jong-Hyun;Jung, Hun;Kim, Jeong-Soo
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2012.05a
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    • pp.143-148
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    • 2012
  • This study is an investigation on macroscopic spray behavior of nonimpinging-type injector equipped on the hydrazine thruster under development. An electron microscope is employed for the acceptance examination of injector orifice. Initial performance characteristics and spray behavior of injector are observed through the instantaneous spray images which are captured by high speed camera and Schlieren method with varying injection pressures. The injector performance is scrutinized by the velocity along with penetration length of spray and categorized by dimensionless parameters. It is confirmed that there exist varying characteristics related to the spray breakup caused by fabrication errors of injector-orifices. Unexpected spray behavior, which needs to be reexamined, is grasped at specific pressure level, as well.

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A study on algal concentration analysis using a fluorescent sensor (형광센서를 활용한 조류 농도 분석에 대한 연구)

  • Lim, Jae Yeon;Kim, Gwang-Su;Nam, Su Han;Kim, Young Do
    • Proceedings of the Korea Water Resources Association Conference
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    • 2022.05a
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    • pp.261-261
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    • 2022
  • 최근 국내 녹조현상의 빈도가 잦아지고, 지속기간이 증가하였다. 이러한 녹조현상은 이상기후로 인한 일사량, 수온, 체류시간 증가로 인한 환경적인 영향이 있으며, 산업단지 및 농지 등에서 유출되는 질소나 인의 부영양화로 인한 인위적 영향이 있다. 조류종 중 특히 남조류가 생장하는 과정에서 발생하는 독성물질(간독소, 신경독소), 이취미 물질(Geosmin, 2-MIB 등)은 수질을 악화시키고 있어 생태계에 큰 영향을 미친다. 조류는 하천에서 넓은 분포로 발생하게 되는데 현재 조류 조사 방법은 현장에 발생한 조류를 직접 채취하여 연구실에서 현미경으로 직접 검경하기 때문에 많은 인력과 시간, 비용이 소비되고 있는 실정이다. 국내에서는 인력과 시간을 줄이기 위해 센서를 활용한 조류분석 및 원격탐사 기법을 이용한 조류 모니터링에 대한 연구가 많이 진행되고 있다. 하지만 하천에서는 녹조류와 남조류 모두 혼합되어 존재하며 센서를 이용하여 조류를 분석할 시 녹조류는 Chl-a, 남조류는 Phycocyanin을 대체하여 분석하고 있다. 하지만 모든 조류는 광합성을 하기 때문에 Chl-a를 모두 함유하고 있어 Chl-a만으로 남조류를 추정하는 데에는 한계가 있다. 따라서 본 연구에서는 조류 계측장비인 LISST-HAB를 통하여 조류시료의 농도를 측정하여 Chl-a, Phycocyanin 농도를 측정하여 조류종 분류에 대한 분석을 진행하였다.

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