• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.92-92
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• 2003

한우사육규모가 다두사육화 되어가고 전업화되면서 발정관찰이 어려워지고 인공수정을 기피하는 현상이 나타나기 시작하였다. 이러한 인공수정 기피현상을 해소하기 위해 발정관찰이 필요없는 정시인공수정, 배란동기화 처리기술 등이 육우 및 유우에서 개발되어 사용되어지고 있으며, 이러한 기술을 한우에 접목시키기 위하여 배란동기화 처리시 적정한 인공수정시기를 구명하고자 본 연구를 수행하였다. 배란동기화 처리는 0일째에 GnRH를 1차 투여하고, 7일째에 PGF$_2$alfa를 투여하였으며, 9일째에 GnRH를 2차 투여하였고 이시점에서 20시간후부터 30시간까지 2시간 간격으로 초음파(Sonovet-600. Medison co., Korea)를 이용하여 난소내 난포발달상태를 촬영하였고 이러한 영상을 통해 난포의 배란시점을 검토한 결과, 공시두수 25두중 24~26시간에 4%(1/25), 26~28시간에 8%(2/25), 28~30시간에 48%(12/25), 30시간 이후에 8%(2/25)가 배란되었고, 계통별 1회 수정 수태율은 고급육계통이 48.1%(38/79), 다유계통이 43.9%(51/91)로 나타났으며, 계절별 1회 수정 수태율은 봄, 여름, 가을 별로 각각 45.8%, 33% 그리고 47.3%로 가을철이 다소 높은 경향이었다.

• Journal of Animal Science and Technology
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• v.51 no.3
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• pp.255-262
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• 2009

Current study was conducted to investigate the influence of parity and region variation in ultrasound live carcass traits of Hanwoo cows. A total of 1,386 heads of Hanwoo cows, spreading over six regions in Gangwon province of South Korea, were measured for longissimus muscle area, back fat thickness, marbling score and rump fat thickness by using real-time ultrasound method. The results revealed that, positive correlations were found in all ultrasound live carcass traits. All live carcass traits were increased in accordance with parity until the sixth, and then decreased thereafter. Statistical significant differences (p<0.01) were found among regions in ultrasound live carcass traits, e.g. Hanwoo cows showed highest longissimus muscle area and marbling score in Hwacheon, while the lowest back fat thickness and rump fat thickness were observed in Yanggu. Greater longissimus muscle area and marbling scores were found in Hanwoo cows which were born during autumn, 2000. Moreover, individuals born during autumn, 2005 and 2006 showed a thicker back fat and rump fat thickness. Back fat thickness had the highest positive correlation with rump fat thickness, while rib eye area and rump fat thickness had the lowest correlation.

• Korean Journal of Ecology and Environment
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• v.46 no.3
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• pp.380-387
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• 2013

The objective of this study is to investigate the effect of elevated $CO_2$ and temperature on the phenology and reproductive ecological characteristics of Bupleurum latissimum. We measured characteristics of 4-year-old B. latissimum grown under control (ambient $CO_2$+ambient temperature, 340~370 ppm) and treatment (elevated $CO_2$+elevated temperature, 690~770 ppm) conditions in a glass house. The period of flowering, fruit appearance and fruit maturing was earlier by 4, 1, 2 days, respectively, under elevated $CO_2$ and temperature. The comparative weight of a single ripe fruit, all ripe fruits and total ripe and unripe fruits was higher per control than treated plants. However the other characteristics, such as no. of peduncle, matured fruits, total fruits per plant and fruit setting rate per plant of B. latissimum were not significantly affected by elevated $CO_2$ and temperature. The germination rate of B. latissimum was higher in control than in treatment plants. These results showed that the phenology of B. latissimuin might be well suited to an earlier growing season, and the response of reproductive characteristics of B. latissimuin is negatively influenced by global warming.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.10
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• pp.433-439
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• 2020

This study, which was designed to improve and increase the utilization of female elk, examined the possibility of collecting their embryos during the non-reproductive period using ultrasound-guided Ovum Pick-Up (OPU) technology. Once follicles in the ovaries of an elk were observed, the embryos were retrieved through the absorption of the follicles using an OPU ultrasound probe. Fifty-seven embryos were retrieved from 85 follicles, giving a retrieval rate of 67.1%. Morphological evaluation of the recovered embryos revealed 14.0% embryos to be in grade A, 19.2% in grade B, 15.7% in grade C, and 50.8% in grade D. The developmental efficiency of the retrieved embryos was also investigated using in vitro maturation, in vitro fertilization, and in vitro culture. After cultivating 28 embryos through in vitro fertilization, 19 embryos were found to be fertilized; the fertilization rate was 67.9%. Four embryos developed to the blastocyst stage, indicating a 14.3% development rate. This study confirmed that the production of fertilized embryos from a seasonal breeder, such as elk, is possible via the ultrasound-guided OPU method. If the efficiency of in vitro fertilization and in vitro culture can be improved through further research, it will help improve the efficiency of elk embryo production through the transplantation of their fertilized embryos.

• Korean Journal of Environment and Ecology
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• v.36 no.6
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• pp.592-602
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• 2022

This study aimed to check habitat distribution and analyze influencing factors by analyzing the mating calls of Auritibicen intermedius inhabiting limited locations in South Korea by applying bioacoustic detection techniques. The study sites were 20 protection areas nationwide. The mating call analysis period was 4 years from 2017 to 2021, excluding 2020. The bioacoustic recording system installed at each study site collected recordings of mating calls every day for 1 minute per hour. Climate data received from the Meteorological Agency, such as temperature, humidity, rainfall, cloudiness, and sunshine, were analyzed. The results of this study identified A. intermedius habitat only in four national parks in the highlands of Gangwon Province (Mt. Seorak, Mt. Odae, Mt. Chiak, and Mt. Taebak) out of 20 study sites. During the four years of study, the mating call period of A. intermedius was between August 5 and September 28, and the duration of the mating call was 31 to 52 days. The temperature analysis during the appearance period of A. intermedius showed that A. intermedius mainly produced mating calls at temperatures between 13.1℃ and 35.3℃, and the average temperature during the circadian cycle of mating calls (09:00 to 16:00) was 24.4 to 24.9℃. The analysis of the circadian cycle of mating calls at four study sites where A. intermedius appeared in 2019 showed that A. intermedius produced mating calls from 06:00 to 16:00 and that they peaked around 11:00 to 12:00. During the appearance period of A. intermedius, four species appeared in common: Hyalessa maculaticollis, Meimuna opalifera, Graptopsaltria nigrofuscata, and Suisha coreana. A logistic regression analysis confirmed that sunlight was the environmental factor affecting the mating call of A. intermedius. Regarding interspecific influence, it was confirmed that A. intermedius exchanged interspecific influence with 4 other common species (H. maculaticollis, M. opalifera, G. nigrofuscata, and S. coreana). The above results confirmed that A. intermedius habitats were limited in the highlands of Gangwon Province highlands in Korea and produced mating calls at a lower temperature compared to other species. These results can be used as basic data for future research on A. intermedius in Korea.

• Korean Journal of Animal Reproduction
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• v.24 no.2
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• pp.209-216
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• 2000

This study was carried out to investigate the effects of breed, age of boar, season, parity and mating system on boar semen characteristics and fertilizing capacity. A total of 4181 sows and 199 boars of Durocs (D), Landraces (L), and Yorkshires (Y) were used for this experiment at Darby Artificicial Insemination Center from 1996 through 1999. Semen volume per ejaculate was largest in Landrace (266.8 $m\ell$), followed by Yorkshire, and was smallest in Duroc. Sperm motility did not show significant differences among the above breeds. Sperm concentration was lowest in Landrace (4.7$\times$10$^{9}$ sperm/$m\ell$) and was highest in Duroc (5.7$\times$10$^{9}$ sperm/$m\ell$). Semen volume per ejaculate according to the age of boars was largest at the age of 2 years, followed by the age of 4 and 3 years, and was smallest at the age of I year. Semen volume per ejaculate according to the season in boars was largest in winter (228.6 $m\ell$), followed by autumn and summer, and was smallest in spring. Sperm concentration was highest in spring (5.9$\times$10$^{9}$ sperm/$m\ell$), followed by summer and winter, and was lowest in autumn. The average litter weight at birth did not show any differences according to the mating type. But the number of pigs born alive per litter was largest (9.5 pigs) in the natural mating ＋ artificial insemination group, followed by the artificial insemination group (9.2 pigs), and was smallest (8.9 pigs) in the natural mating group (P＜0.01). The average litter weight at birth and number of pigs born alive per litter did not show any differences between the natural mating and artificial insemination. The L (♀)$\times$Y (♂) and L (♀)$\times$L (♂) matings show $\varepsilon$ d higher average litter weight at birth and number of pigs born alive per litter than the Y (♀) $\times$ Y (♂) and Y (♀) $\times$ L (♂) matings. The pigs in the 2~6th parities had higher average litter weight at birth and number of pigs born alive per litter than those in the 1 st and 7~9th parities.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.129-129
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• 2003

번식효율이 높은 우수한 후보종빈돈의 조기선발기술을 개발하기 위해 후보종빈돈의 첫발정일령과 첫수정일령에 미치는 요인을 분석한 결과는 다음과 같다. 본 시험에 공시된 후보종빈돈은 충남 천안시에 위치하고 있는 양돈장에서 자돈을 생산ㆍ육성하여 체중 80~90kg에서 선발하였고, 체중이 약 110kg 도달하였을 때 최종 선발하여 이용하였으며, 사양관리는 양돈장의 관행에 따라 실시하였다. 첫발정조사는 생후 22주령때 부터 매일 아침, 저녁으로 2회씩 외음부의 충혈과 부종상태를 관찰하고 승가허용 자세유지 등을 통하여 발정여부를 조사하였다. 교배는 첫발정발견 후 2차 발정이 발현되었을 때 실시하는 것을 원칙으로 하였다. 첫발정 및 첫수정시에 등지방측정기(Lean-meater; Renco, U.S.A.)를 이용하여 제 10늑골의 정중선으로부터 좌측 또는 우측으로 약 5cm 이격된 지점을 2회 측정하여 평균치로 하였다. 후보종빈돈의 품종별 첫발정일령과 첫수정일령은 랜드레이스종이 171.91일과 202.18일, 요크셔종은 178.56일과 208.39일, 랜드레이스종과 요크셔종의 F$_1$은 190.20일과 213.60일로 랜드레이스종이 첫발정일령과 첫수정일령이 가장 빨랐으나 품종간 첫수정일령에 대한 유의적인 차이는 없었다. 출생계절별 첫발정일령 및 첫수정일령은 봄에 출생한 후보종빈돈은 194.14일 및 222.05일, 여름에 출생한 후보종빈돈은 163.25일 및 193.00일, 가을에 출생한 후보종빈돈은 160.25일 및 199.20일, 겨울에 출생한 후보종빈돈은 159.72일 및 190.11일로 봄에 출생한 후보종빈돈의 첫발정일령 및 첫수정일령이 겨울, 가을, 여름에 출생한 후보종빈돈보다 유의적으로 늦게 나타났다(P〈0.01). 등지방두께가 13~16mm인 후보종빈돈의 첫발정일령 및 첫수정일령은 180.32일 및 211.12일, 17~20mm인 후보종빈돈은 172.24일 및 202.43일, 21~23mm인 후보종빈돈은 162.20일 및 195.43일로 등지방두께가 얇을수록 첫발정일령 및 첫수정일령이 지연되었으나 유의적인 차이는 인정되지 않았다.

• Journal of Veterinary Clinics
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• v.28 no.4
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• pp.339-343
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• 2011

This study determined the effects of twin birth on subsequent postpartum disorders, culling and reproductive performance, and its risk factors in dairy cows. Detailed data related to reproduction, health, and calving events, including dates of artificial insemination (AI) and calving, hormonal treatment prior to conception, cow parity, twin birth, retained placenta, metabolic disorders, and endometritis were collected from 1,717 individual calvings on 9 dairy farms. The incidence rate of twin birth was 3.4%. Gestation length was shorter in cows with twin birth ($270.6{\pm}2.0$ days) than cows giving birth to singletons ($279.5{\pm}0.2$ days, P < 0.01). The incidence of retained placenta (47.5 vs. 16.0%), metabolic disorders (18.6 vs. 3.8%) endometritis (62.7 vs. 28.2%), and culling rate (32.2 vs. 16.5%) was greater in cows with twin birth than in cows giving birth to singletons, respectively (P < 0.01). The hazard of pregnancy (adjusted hazard ratio, AHR) by 210 days in milk analyzed using Cox proportional hazards model was affected by calving season, in that the rate of pregnancy for cows calved during spring was less than for cows calved during winter (AHR = 0.80; P = 0.01); this was also true for endometritis (AHR = 0.46, P < 0.01). AHR was not affected by twin birth. Logistic analysis demonstrated that increasing the cow parity (P < 0.01) and hormonal treatment prior to conception (adjusted odds ratio, AOR = 1.84, P < 0.05) increased the risk of twin birth. These results demonstrate that twin births are responsible for severe economic losses through the increased occurrence of postpartum disorders and culling; thus, appropriate management for the deleterious impact of twin birth, and/or reducing the risk factors by herd control regarding cow parity and the use of reproductive hormones prior to conception might be necessary.

• Korean Journal of Environmental Biology
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• v.41 no.3
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• pp.298-307
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• 2023

The annual reproductive cycle of two species, Upogebia major (de Haan 1841) and Austinogebia wuhsienweni (Yu 1931), of the female mud shrimp from the west coast of Korea was investigated using histology. The collected samples were divided into adult and juvenile groups to understand the mature period of age class based on the carapace length(CL). Juvenile Upogebia(CL<25mm) were mostly inactive gonad with early (62%-100%) and late (10%-38%) development stages during the year, whereas the adult shrimp showed a seasonal pattern of gonad maturation(CL≥25 mm). The early and late developmental stages of oocytes were observed in adult Upogebia from November to March and mature eggs appeared from April to October. In adult Ausitnogebia (CL≥15 mm), fully grown oocytes were consistently observed during the study period, in which the ripe stage was found between January and June. On the other hand, most juvenile Austinogebia (CL<15 mm) maintained an immature state in the gonad. Both species of the mud shrimp reproduced from ovigerous females in the adult population and their egg-bearing period was distinguished from January to April for U. major and from July to September for A. wuhsienweni.

• Korean Journal of Environment and Ecology
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• v.35 no.6
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• pp.594-600
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• 2021

The purpose of this study is to observe the period of mating calls of cicadas in South Korea to identify the emergence period and geographic distribution for each cicada species. The study sites were 19 protection areas nationwide. The mating calls of cicadas were collected over the 12 months of 2019. A bioacoustics measuring device was installed to record the mating calls of cicadas in WAV, 44,100Hz format for 1 minute every hour. The temperature was recorded once or twice every hour using a micro-meteorological measuring device. Nine species of Korean cicadinae were studied. The start and end periods of mating calls were recorded for each cicada species for the subsequent analysis. The analysis results showed that nine cicada species appeared in the 19 protection areas. The chronological order of mating call periods for each species was as follows: Cryptotympana atrata (7/12 - 9/30), Meimuna opalifera (7/27 - 10/20), Hyalessa fuscata (7/25 - 10/9), Graptopsaltria nigrofuscata (7/28 - 9/5), Platypleura kaempferi (7/3 - 9/29), Suisha coreana (9/14 - 10/30), Leptosemia takanonis (6/26 - 8/2), Auritibicen intermedius (7/27 - 9/28), and Meimuna mongolica (8/8 - 9/11). The mating call period was between 35 (Meimuna mongolica) and 89 (Platypleura kaempferi) days, with the average being 62 days. The elevation above sea level for the habitats of each species was as follows: 5 - 386 m for Cryptotympana atrata, 7 - 759 m for Meimuna opalifera, 7 - 967 m for Hyalessa fuscata, 42 - 700m for Graptopsaltria nigrofuscata, 7 - 700 m for Platypleura kaempferi, 5 - 759 m for Suisha coreana, 7 - 759 m for Leptosemia takanonis, 397 - 967 m for Auritibicen intermedius, and 7 - 42 m for Meimuna mongolica. The average temperature of the habitats of each species was as follows: 23.9℃ for Cryptotympana atrata, 21.8℃ for Meimuna opalifera, 22℃ for Hyalessa fuscata, 23℃ for Graptopsaltria nigrofuscata, 22.9℃ for Platypleura kaempferi, 14.6℃ for Suisha coreana, 20.6℃ for Leptosemia takanonis, 19.3℃ for Auritibicen intermedius, and 24.4℃ for Meimuna mongolica. In terms of the habitat distribution of species, Meimuna opalifera, Hyalessa fuscata, and Platypleura kaempferi were distributed in more than 15 protection sites. Cryptotympana atrata was distributed in the lowlands in the southwest. Graptopsaltria nigrofuscata was distributed in the western area of the Korean Peninsula. Suisha coreana was distributed in areas excluding high mountain areas and parts of the southeast area. Leptosemia takanonis was distributed in areas near the mountains. Auritibicen intermedius was distributed locally in the high mountain areas. Meimuna mongolica was distributed locally in flat wetlands.