The fracture toughness test is believed as a clinically relevant method for assessing the fracture resistance of the dentinal restoratives. The objectives of this study were to measure the fracture toughness $(K_{1C})$ and microtensile bond strength of dentin-resin composite interface and compare their relationship for their use in evaluation of the integrity of the dentin-resin bond. A minimum of six short-rod specimens for fracture toughness test and fifteen specimens for microtensile bond strength test was fabricated for each group of materials used. After all specimens storing for 24 hours in distilled water at $37^{\circ}C$, they were tensile-loaded with an EZ tester universal testing machin. Statistical analysis was performed using ANOVA and Tukey's test at the 95% confidence level, Pearson's coefficient was used to verify the correlation between the mean of fracture toughness and microtensile bond strength. FE-SEM was employed on fractured surface to describe the crack propagation. Fracture toughness value of Clearfil SE Bond (SE) was the highest, followed by Adper Single Bond 2 (SB), OptiBond Solo (OB), ONE-STEP PLUS (0S), ScotchBond Multi-purpose (SM) and there was significant difference between SE and other 4 groups (p < 0.05). There were, however, no significant difference among SB, OB, OS, SM (p > 0.05). Microtensile bond strength of SE was the highest, followed by SB, OB, SM, OS and OS only showed significant lower value (p < 0.05). There was no correlation between fracture toughness and microtensile bond strength values. FE-SEM examination revealed that dentin bonding agent showed different film thickness and different failure pattern according to the film thickness. From the limited results of this study, it was noted that there was statistically no correlation between K1C and ${\mu}TBS$. We can conclude that for obtaining the reliability of bond strength test of dentin bonding agent, we must pay more attention to the test procedure and its profound scrutiny.
H.264| MPEG-4 AVC is a new video codingstandard defined by JVT (Joint Video Team) which consists of ITU-T and ISO/IEC. Many techniques are adopted fur the compression efficiency: Especially, an intra prediction in an inter frame is one example but it leads to excessive amount of encoding time due to the decision of a candidate mode and a RDcost calculation. For this reason, a fast determination of the best intra prediction mode is the main issue for saving the encoding time. In this paper, by using the result of statistical relation between intra $16{\times}16$ and $4{\times}4$ intra predictions, the number of candidate modes for $4{\times}4$ intra prediction is reduced. Firstly, utilizing motion vector obtained after inter prediction, prediction of a block mode for each macroblock is made. If an intra prediction is needed, the correlation table between $16{\times}16$ and $4{\times}4$ intra predicted modes is created using the probability during each I frame-coding process. Secondly, using this result, the candidate modes for a $4{\times}4$ intra prediction that reaches a predefined specific probability value are only considered in the same GOP For the experiments, JM11.0, the reference software of H.264|MPEG-4 AVC is used and the experimental results show that the encoding time could be reduced by 51.24% in maximum with negligible amounts of PSNR drop and bitrate increase.
Genetic map and molecular marker have a great importance in improving and facilitating crop breeding program as well as in genome analysis and map-based cloning of genes representing desirable characters. This study aimed at developing RAPD markers and constructing a genetic linkage map using 82 BC$_1$F$_1$individuals originated from the cross between '835' and B$_2$in radish (Raphanus sativus L.). One of the parents for genetic linkage map construction, '835'(P$_1$) of egg type is susceptible to Fusarium wilt and have medium resistance to virus infection and the other parent, B$_2$(P$_2$) of round type, is susceptible to Fusarium wilt and virus, Screening of 394 RAPD primers in BC$_1$F$_1$) population resulted in selecting 128 polymorphic markers which displayed 1:1 segregation pattern. Two markers failed to display 1:1 segregation and showed the segregation ratio skewed to maternal genotype. Selected markers were categorized into 14 linkage group based on LOD score represented by MAPMAKER/EXP program. Five groups composed of single marker among them were excluded from the linkage map, and consequently, the remaining groups are well matched with the number of radish chromosome (n=9). The linkage map constructed with 128 markers covers 1,688.3 cM and the average distance between markers was 13.8 cM. For developing STS marker, we determined the partial nucleotide sequence of OPE10 marker at both ends and designed a oligonucleotide primer pair based on this sequence. STS PCR using the primer pair displayed a single, clear band of which segregation is perfectly matched with that of OPE10 marker. This implies that RAPD markers could readily convert into clear and reliable STS markers.
Purpose: The purpose of this study is to evaluate migration of technetium-99m hexamethylpropylene amine oxime ($^{99m}Tc$-HMPAO) labeled immature and mature dendritic cells (DC) in the mouse. Methods: DC were collected from bone marrow (BM) of tibiae and femurs of mice. Immature and mature DC from BM cells were radiolabeled with $^{99m}Tc$-HMPAO. To evaluate the functional and phenotypic changes of DC from radiolabeling, the allogeneic mixed lymphocyte reaction (MLR) and fluorescence-activated cell sorting (FACS) analysis were performed before and after labeling with $^{99m}Tc$-HMPAO. Migration of intravenously injected DC (iv-DC) was assessed by serial gamma camera images of mice with or without subcutaneous tumor. Percent injected dose per gram (%ID/g) was calculated in lungs, liver, spleen, kidneys, and tumor through dissection of each mice after 24 hours of injection. Results: Labeling efficiency of immature and mature DC were $60.4{\pm}5.4%\;and\;61.8{\pm}6.7%$, respectively. Iv-DC initially appeared in the lungs, then redistributed mainly to liver and spleen. Migration of mature DC to spleen was significantly higher than that of immature DC ($38.3{\pm}4.0%\;vs.\;32.2{\pm}4.1%$ in control group, $40.4{\pm}4.1%\;vs.\;35.9{\pm}3.8%$ in tumor group; p<0.05). Migration to tumor was also significantly higher in mature DC than in immature DC ($2.4{\pm}0.3%\;vs\;1.7{\pm}0.2%$; p=0.034). Conclusion: Assessment of migration pattern of DC in mice was possible using $^{99m}Tc$-HMPAO labeled immature and mature DC. Migration of mature DC to spleen and tumor was higher than that of immature DC when they were i.v. injected.
Journal of the Korean Society of Fisheries and Ocean Technology
/
v.34
no.1
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pp.74-84
/
1998
A doubly resonant ultrasonic transducer has been designed as an attempt to increase the bandwidth of underwater transducers. The dual resonance conditions were accomplished by attaching a single acoustic matching layer on the front face of a Tonpilz transducer consisted of an aluminum head, a piezoelectric ring, a brass tail and a prestress bolt. A modified Mason's model was used for the performance analysis and the design of transducers, and the constructed transducers were tested experimentally and numerically by changing the impedances and thicknesses of the head, tail and matching layers in the water tank. Two distinct resonance peaks in the transmitting voltage response(TVR) of a developed transducer were observed at 34.3 and 40.4 kHz, respectively, with the difference frequency of 6.1kHz and the center frequency of 37.2kHz. The values of TVR at these frequencies were 136.5 dB re $1\;\muPa/V$ at 34.3 kHz and 136.8 dB re $1\;\muPa/V$ at 40.4 kHz, respectively. Reasonable agreement between the experimental results and the numerical results was achieved. From this result, it is expected that the generation of the distinct resonances at any two desired frequencies can be achieved through the proper choice of the matching layer to provide the impedance transformation between the transducer and the medium.
Ground-based remote sensing can be used as one of the non-destructive, fast, and real-time diagnostic tools for quantifying yield, biomass, and nitrogen (N) stress during growing season. This study was conducted to assess biomass and nitrogen (N) status of paddy rice (Oryza sativa L.) plants under N stress using passive and active ground-based remote sensors. Nitrogen application rates were 0, 70, 100, and 130 kg N $ha^{-1}$. At each growth stage, reflectance indices measured with active sensor showed higher correlation with DW, N uptake and N concentration than those with the passive sensor. NIR/Red and NIR/Amber indices measured with Crop Circle active sensors generally had a better correlation with dry weight (DW), N uptake and N content than vegetation indices from Crop Circle passive sensor and NDVIs from active sensors. Especially NIR/Red and NIR/amber ratios at the panicle initiation stage were most closely correlated with DW, N content, and N uptake. Rice grain yield, DW, N content and N uptake at harvest were highly positively correlated with canopy reflectance indices measured with active sensors at all sampling dates. N application rate explains about 91~92% of the variability in the SI calculated from NIR/Red or NIR/Amber indices measured with Crop Circle active sensors on 12 July. Therefore, the in-season sufficiency index (SI) by NIR/Red or NIR/Amber index from Crop Circle active sensors can be used for determination of N application rate.
This study investigated the seed and water absorption characteristics of red bean cultivars in Korea. The length and width of all seeds were 7.74-8.99 mm and 5.33-5.54 mm, respectively. The hilum lengths and widths were 3.21-4.01 mm and 0.58-0.73 mm, respectively. The weight of 100 seeds was 12.14-20.21 g, and the seed coat thickness of red beans ranged from 39.18-50.01 mm. During soaking for 30 h, red beans exhibited a lag phase in the initial soaking time, followed by a rapid absorption phase, and finally contained more than 130% moisture. When water absorption was applied to the sigmoid equation, the curve fitting exhibited a high R2, and the kinetics of water absorption were 0.202-0.715 h-1, which differed among varieties. Pearson's correlation of hilum width (r=0.7858*) and seed thickness (r= -0.9954***) exhibited strong correlations with water absorption, suggesting that these are important factors in red bean processing.
Journal of the Korean Society of Marine Environment & Safety
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v.27
no.2
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pp.387-393
/
2021
In this study, finite element analysis was performed to evaluate a method of increasing the fatigue life of the pipe connection structure commonly used in the topside structure of offshore platforms. MSC Patran/Nastran, a commercial analysis program, was used, and the critical structural model was selected from the global analysis. To realize the stress concentration phenomenon according to the load, modeling using 8-node solid elements was implemented. The main loads were considered to be two lateral loads and a tensile load on a diagonal pipe. To check the hotspot stress at the main location, a 0.01 mm dummy shell element was applied. After calculating the main stress at the 0.5-t and 1.5-t locations, the stress generated in the weld was estimated through extrapolation. In some sections, this stress was observed to be below the fatigue life that should be satisfied, and reinforcement was required. For reinforcement, a bracket was added to reduce the stress concentration factor where the fatigue life was insufficient without changing the thickness or diameter of the previously designed pipe. Regarding the tensile load, the stress in the bracket toe increased by 23 %, whereas the stress inside and outside of the pipe, which was a problem, decreased by approximately 8 %. Regarding the flexural load, the stress at the bracket toe increased by 3 %, whereas the stress inside and outside of the pipe, which was also a problem, decreased by approximately 48 %. Owing to the new bracket reinforcement, the stress in the bracket toe increased, but the S-N curve itself was better than that of the pipe joint, so it was not a significant problem. The improvement method of fatigue life is expected to be useful; it can efficiently increase the fatigue life while minimizing changes to the initial design.
Park, DaeKyeong;Shin, DongIl;Shin, DongKyoo;Kim, Sangsoo
KIPS Transactions on Software and Data Engineering
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v.10
no.7
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pp.271-278
/
2021
As the current cyber attacks become more intelligent, the existing Intrusion Detection System is difficult for detecting intelligent attacks that deviate from the existing stored patterns. In an attempt to solve this, a model of a deep learning-based intrusion detection system that analyzes the pattern of intelligent attacks through data learning has emerged. Intrusion detection systems are divided into host-based and network-based depending on the installation location. Unlike network-based intrusion detection systems, host-based intrusion detection systems have the disadvantage of having to observe the inside and outside of the system as a whole. However, it has the advantage of being able to detect intrusions that cannot be detected by a network-based intrusion detection system. Therefore, in this study, we conducted a study on a host-based intrusion detection system. In order to evaluate and improve the performance of the host-based intrusion detection system model, we used the host-based Leipzig Intrusion Detection-Data Set (LID-DS) published in 2018. In the performance evaluation of the model using that data set, in order to confirm the similarity of each data and reconstructed to identify whether it is normal data or abnormal data, 1D vector data is converted to 3D image data. Also, the deep learning model has the drawback of having to re-learn every time a new cyber attack method is seen. In other words, it is not efficient because it takes a long time to learn a large amount of data. To solve this problem, this paper proposes the Siamese Convolutional Neural Network (Siamese-CNN) to use the Few-Shot Learning method that shows excellent performance by learning the little amount of data. Siamese-CNN determines whether the attacks are of the same type by the similarity score of each sample of cyber attacks converted into images. The accuracy was calculated using Few-Shot Learning technique, and the performance of Vanilla Convolutional Neural Network (Vanilla-CNN) and Siamese-CNN was compared to confirm the performance of Siamese-CNN. As a result of measuring Accuracy, Precision, Recall and F1-Score index, it was confirmed that the recall of the Siamese-CNN model proposed in this study was increased by about 6% from the Vanilla-CNN model.
An, Na-Rae;Son, Ju-Hwan;Park, Jong-Eun;Chai, Han-Ha;Jang, Gul-Won;Lim, Dajeong
Journal of Life Science
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v.28
no.11
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pp.1255-1261
/
2018
Whole genome analysis have been made possible with the development of DNA sequencing technologies and discovery of many single nucleotide polymorphisms (SNPs). Large number of SNP can be analyzed with SNP chips, since SNPs of human as well as livestock genomes are available. Among the various missing nucleotide imputation programs, Minimac3 software is suggested to be highly accurate, with a simplified workflow and relatively fast. In the present study, we used Minimac3 program to perform genomic missing value substitution 1,226 animals 770K SNP chip and imputing missing SNPs with next generation sequencing data from 311 animals. The accuracy on each chromosome was about 94~96%, and individual sample accuracy was about 92~98%. After imputation of the genotypes, SNPs with R Square ($R^2$) values for three conditions were 0.4, 0.6, and 0.8 and the percentage of SNPs were 91%, 84%, and 70% respectively. The differences in the Minor Allele Frequency gave $R^2$ values corresponding to seven intervals (0, 0.025), (0.025, 0.05), (0.05, 0.1), (0.1, 0.2), (0.2, 0.3). (0.3, 0.4) and (0.4, 0.5) of 64~88%. The total analysis time was about 12 hr. In future SNP chip studies, as the size and complexity of the genomic datasets increase, we expect that genomic imputation using Minimac3 can improve the reliability of chip data for Hanwoo discrimination.
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