• Journal of Life Science
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• v.28 no.11
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• pp.1379-1385
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• 2018

Glycans are attached to proteins as in glycoproteins and proteoglycans. They are found on the exterior surface of cells. O- and N-linked glycans are very common in eukaryotic cells but may also be found in prokaryotes. The interaction of cell surface glycans with complementary glycan binding proteins located on neighboring cells, other cell types, pathogens like virus, or bacteria is crucial in biologically and biomedically important processes like pathogen recognition, cell migration, cell-cell adhesion, development, and infection. Their implication in pathological condition, suggests an important role for glycans as disease markers. In addition, a great amount of research has been shown that appropriate glycosylation of a recombinant therapeutic protein is critical for product solubility, stability, pharmacokinetics and pharmacodynamics, bioactivity, and safety. Besides, cancer-associated glycosylation changes often involve sialic acid in glycan branch which play important roles in cell-cell interaction, recognition and immunological response. This review aims at giving a comprehensive overview of the glycan's biological function and describing the relevance among the glycosylation, disease diagnosis and treatment methods. Furthermore, the high-throughput analytic methods available to measure the profile changing patterns of glycan in the blood serum as well as possible underlying biochemical mechanisms.

• Journal of Life Science
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• v.26 no.3
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• pp.282-288
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• 2016

Protein-protein interactions regulate the subcellular localization and function of receptors, enzymes, and cytoskeletal proteins. Proteins containing the postsynaptic density-95/disks large/zonula occludens-1 (PDZ) domain have potential to act as scaffolding proteins and play a pivotal role in various processes, such as synaptic plasticity, neural guidance, and development, as well as in the pathophysiology of many diseases. Multi-PDZ domain protein 1 (MUPP1), which has 13 PDZ domains, has a scaffolding function in the clustering of surface receptors, organization of signaling complexes, and coordination of cytoskeletal dynamics. However, the cellular function of MUPP1 has not been fully elucidated. In the present study, a yeast two-hybrid system was used to identify proteins that interacted with the N-terminal PDZ domain of MUPP1. The results revealed an interaction between MUPP1 and Wdpcp (formerly known as Fritz). Wdpcp was identified as a planar cell polarity (PCP) effector, which is known to have a role in collective cell migration and cilia formation. Wdpcp bound to the PDZ1 domain but not to other PDZ domains of MUPP1. The C-terminal end of Wdpcp was essential for the interaction with MUPP1 in the yeast two-hybrid assay. This interaction was further confirmed in a glutathione S-transferase (GST) pull-down assay. When coexpressed in HEK-293T cells, Wdpcp was coimmunoprecipitated with MUPP1. In addition, MUPP1 colocalized with Wdpcp at the same subcellular region in cells. Collectively, these results suggest that the MUPP1-Wdpcp interaction could modulate actin cytoskeleton dynamics and polarized cell migration.

• Journal of Life Science
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• v.33 no.1
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• pp.1-7
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• 2023

Intracellular cargo transport is mediated by molecular motor proteins, such as kinesin and cytoplasmic dynein. Kinesins make up a large subfamily of molecular motors. Kinesin-1 is a plus-end-directed molecular motor protein that moves various cargoes, such as organelles, protein complexes, and mRNAs, along a microtubule track. It consists of the kinesin superfamily protein (KIF) 5A, 5B, and 5C (also called kinesin heavy chains) and kinesin light chains (KLCs). Kinesin-1 interacts with many different binding proteins through its carboxyl (C)-terminal region of KIF5s and KLCs, but their binding proteins have not yet been fully identified. In this study, a yeast two-hybrid assay was used to identify the proteins that interact with the KIF5A specific C-terminal region. The assay revealed an interaction between KIF5A and glutamate-rich 4 (ERICH4). ERICH4 bound to the KIF5A specific the C-terminal region but did not interact with the C-terminal region of KIF5B or KIF3A (a motor protein of kinesin-2). In addition, KIF5A did not interact with another isoform, ERICH1. Glutathione S-transferase (GST) pull-downs showed that KIF5A interacts with GST-ERICH4 and GST-ERICH4-amino (N)-terminal but not with GST-ERICH4-C or GST alone. When co-expressed in HEK-293T cells, ERICH4 co-localized with KIF5A and co-immunoprecipitated with KIF5A and KLC but not KIF3B. Together, our findings suggest that ERICH4 is capable of binding to KIF5A and that it may serve as an adaptor protein that links kinesin-1 with cargo.

• Journal of the Korean Society for information Management
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• v.31 no.1
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• pp.53-76
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• 2014

Combining makerspaces and library services is an innovative idea that could positively and radically change the library user experience. The Library Makerspace is able to offer users a different view of the world, and give them the opportunity to explore and imagine new possibilities which they can then create. Therefore, this study focused on analyzing real-world makerspace examples to create suggests for utilizing this trend. The roles and concepts of library makerspaces derived through this research are as follows: space for infinite creativity as social communication space, study areas, job searches, self-publishing, idea incubation, collaboration, equipment and utilization, storytelling, expert mentoring and consulting, and so on. The possibilities are endless, and allow for the development and expansion of the library.

• Journal of the Korean association of regional geographers
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• v.16 no.5
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• pp.457-473
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• 2010

Patterns and regional distribution of disaster-related place names have been analyzed to confirm the recognition and probability of disaster and to explore the possibility of disaster prevention measures. 106 terms and 37,901 place names related to disaster and prevention measures have been collected from the Korean gazetteers "Hanguk Jimyeong Chongnam". Based on this, some conclusions have been drawn: firstly, place names related to the geomorphic processes and prevention measures are more common than any other disasters; secondly, place names related to heavy rain, flooding and drowning are most common. Analysis of the regional distribution pattern shows that disaster-related place names are most common in Jeolla and Gyeongsang Provinces and general place names reflecting environmental concern such as water, sand, plain, rain and dam are distributed evenly throughout the whole country; howe, r, place names such as dumbeong, gureong, yeoul, tan(灘), bangjuk, je(堤), and ji(池) are restricted to the specific region, which shows that place names reflects the locational and toprn sucic ainuations. Case st, anindicates that prevention measures should be focused on tributaries and srill villeys conaid ring that disasters originated from the combination of weather and landform conditions are most common throughout the whole country.

• Korea and Global Affairs
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• v.1 no.1
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• pp.97-114
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• 2017

This article aims for searching the clue of North Korea nuke-problem by understanding it contextually. We must see this from the beginning as many trials in settling the problem have failed. North Korea nuke-problem is related to the change of international implication as well as the domestic process of its development. At first, it started from the regime survival, which evolved with the effect of learning. During the Cold War, its development had combined the principle of 'self-defense' because of Sino-Russia conflict. Particularly, its meaning varied dramatically due to the collapse of Cold War system plus the advent of post-Cold War system. North Korea nuclear program has became a global problem based on the deterioration of North Korea's security milieu and Bush administration's policy. Therefore, the area of common solution for NK nuke-problem must be found in the context of regime survival and the change of its meaning by continual convergence effort.

• Journal of KIISE:Software and Applications
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• v.31 no.6
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• pp.760-772
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• 2004

Component Based Development(CBD) Methodology is widely used in software development lifecycle to improve software quality. The Component Based Development(CBD) results to improve software reusability and reduce development term and cost. For this reason, lots of Enterprises are trying to make their processes to components. But, there has been few quality assurance or reusability testing action to those components. Most software component users can not know how their components are reusable and what extent their components satisfy to th eir quality requirements. For this reason, this paper suggests that software components can be measured their reusability by metrics proposed by this paper. We propose that in measuring software component reusability, there are direct metrics and indirect metrics. The results made by direct metrics are suggested to measure indirect metrics, so results to obtain reusability metrics.

• Journal of Digital Convergence
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• v.15 no.5
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• pp.373-380
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• 2017

Recently, virtual reality contents are being experimented and introduced in various fields such as game, education, medical, exhibition, movie, SNS. However, research on virtual reality is mainly focused on industrial and technological perspectives, so contents-centric or contents-oriented research is on virtual reality is very rare. In order to prepare for the future of virtual reality properly, content-oriented research is very much needed. The purpose of this study is to investigate the types, characteristics, and possibilities of virtual reality contents applied to theme parks. For this purpose, we tried to classify the contents type of virtual reality based theme park. The criteria of type classification are divided into 'fixed type', 'continuous moving type', and 'segmented moving type' according to the player's position movement possibility and movement type, here we combine the senses such as tactile, taste, smell, balance etc.

• Journal of Korean Society for Geospatial Information Science
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• v.19 no.3
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• pp.33-40
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• 2011

This paper presents the prediction and evaluation of landslide using LRA(logistic regression analysis) and ANN (Artificial Neural Network) methods. In order to assess the landslide, we selected Sarabong, Byeoldobong area and Mt. Song-ak in Jeju Island. Five factors which affect the landslide were selected as: slope angle, elevation, porosity, dry density, permeability. So as to predict and evaluate the landslide, firstly the weight value of each factor was analyzed by LRA(logistic regression analysis) and ANN(Artificial Neural Network) methods. Then we got two prediction maps using AcrView software through GIS(Geographic Information System) method. The comparative analysis reveals that the slope angle and porosity play important roles in landslide. Prediction map generated by LRA method is more accurate than ANN method in Jeju. From the prediction map, we found that the most dangerous area is distributed around the road and path.

• Journal of Korea Water Resources Association
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• v.54 no.9
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• pp.705-718
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• 2021

Climate change has a significant impact on vegetation growth and terrestrial ecosystems. In this study, the possibility of ecological drought was investigated using satellite remote sensing data. First, the Vegetation Health Index was estimated from the Normalized Difference Vegetation Index and Land Surface Temperature provided by MODIS. Then, a joint probability model was constructed to estimate the possibility of vegetation-related drought in various precipitation/evaporation scenarios in forest areas around 60 major ASOS sites of the Meteorological Administration located throughout Korea. The results of this study show the risk pattern of drought related to forest vegetation under conditions of low atmospheric moisture supply or high atmospheric moisture demand. It also identifies the sensitivity of drought risks associated with forest vegetation under various meterological drought conditions. These findings provide insights for decision makers to assess drought risk and develop drought mitigation strategies related to forest vegetation in a warming era.