• Analytical Science and Technology
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• v.28 no.1
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• pp.65-71
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• 2015

Intracellular organelles in eukaryotic cells play important roles in many cellular functions. Intracellular trafficking of many proteins to specific intracellular organelles is tightly regulated by various mechanisms in cells. Therefore, elucidating the targeting mechanism of novel markers for intracellular organelles is important for cellular physiology and pathology. In this study, we tried to identify the peptides which could bind to specific glycolipid in cellular membrane using GFP-fused glycolipid-binding peptides, and analyzed their cellular localization. As a result, we could identify mitochondria-, Golgi- or plasma membrane-targeting peptides. Furthermore, we found that the plasma membrane-targeting peptide was localized to the plasma membrane via electrostatic interactions. Thus, our results suggest that various glycolipid-binding peptides could be used as intracellular organelles markers.

• Polymer(Korea)
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• v.35 no.2
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• pp.119-123
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• 2011

To develop hyaluronic acid (HA)-based anticancer agent carrier, hyaluronic acid was chemically modified with the hydrophobic group of deoxycholic acid(DA). The physicochemical properties of the deoxycholic acid-conjugated HA (HADA) were investigated by using $^1H$ NMR, FTIR spectrophotometer and TEM. Paclitaxel (Tx)-loaded HADA nanoparticles were prepared by a dialysis method. The loading efficiency of drug and drug contents of Tx-loaded HADA nanoparticles (HADA-Tx) were measured by HPLC. The anticancer activity of HADA-Tx was investigated by its cytotoxicity against KB cell in vitro. The HADA-Tx was shown to have the superior potential for the anticancer drug delivery.

• Journal of Life Science
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• v.18 no.3
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• pp.298-303
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• 2008

Using a phage peptide library approach, we have isolated a peptide ligand that binds to transferrin receptor on the surface of human melanoma cell, B16F10. The library was first screened twice by recovering internalized phages and was further screened three times by competitively eluting transferrin receptor-specific phages with human transferrin among the phages bound to the cell surface. The peptides displayed by the selected phages were fused to translocation and catalytic domain of Pseudomonas exotoxin to prepare recombinant toxins. After estimating cytotoxicity of each recombinant toxin toward B16F10 cell, seven clones were selected. Sequence analysis revealed that one of the clones displayed a peptide which had a significant sequence homology with human transferrin. The peptide was chemically synthesized and was shown to be functional in delivering cytotoxic agents into B16F10 cell via interaction with transferrin receptor.

• Applied Biological Chemistry
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• v.45 no.3
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• pp.162-167
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• 2002

Free, soluble esterified and insoluble bounded phenolic acids were separated from Eungi chestnut endoderm. The composition and contents of phenolic acid were analyzed by gas chromatography, and their antioxidant activity was examined by DPPH assay, 2-deoxyribose oxidation, and ferric thiocyanate method. Gallic, ellagic, salicylic, and gentisic acids in free phenolic acid fraction, gallic, ellagic, and protocatechuic acids in soluble esterified fraction, sianpic and gentisic acids were the major phenolic acids in insoluble bounded fraction. Marked differences were observed in the phenolic acid composition and contents among the fractions. Free phenolic acid fraction showed the strongest antioxidant activity. Results revealed chestnut endoderm could be a potential antioxidant source containing gallic and ellagic acids.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.17 no.2
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• pp.1-6
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• 2017

Due to the recent advances in IoT technologies, reducing power consumption in battery-based IoT devices becomes an important issue. An IoT device is a kind of real-time systems, and processor voltage scaling is known to be effective in reducing power consumption. However, recent research has shown that power consumption in memory increases dramatically in such systems. This paper aims at combining processor voltage scaling and low-power NVRAM technologies to reduce power consumption further. Our main idea is that if a task is schedulable in a lower voltage mode of a processor, we can expect that the task will still be schedulable even on slow NVRAM memory. We incorporate the NVRAM memory allocation problem into processor voltage scaling, and evaluate the effectiveness of the combined approach.

• Journal of the Korean Magnetics Society
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• v.21 no.4
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• pp.132-135
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• 2011

The exchange bias field ($H_{EX}$) and the coercivity ($H_C$) variation and change depending on the thickness of intermediately super-soft magnetic NiFeCuMo layer with different thickness of the bottom NiFe layer were investigated. The $H_{EX}$ of triple pinned NiFe(4 nm)/NiFeCuMo($t_{NiFeCuMo}$= 1 nm)/NiFe(4 nm)/FeMn multilayer has the maximum value more less than one of single pinned NiFe(8 nm)/FeMn layer. If NiFeCuMo layer is inserted each into between the pinned and free NiFe layers, we can be used as GMR-SV device for a bio-sensor that has improved magnetic sensitivity.

• Applied Chemistry for Engineering
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• v.22 no.1
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• pp.114-118
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• 2011

Even though the carbon paste electrode bound with mineral oil is useful for research about the characteristics of enzymes, it remains far from practical uses because the lack of mechanical hardness limits its practical use. When the rubber liquefied in toluene was used as a binder of carbon powder in lab, it is confirmed that the mechanical robustness of the electrode is guaranteed. In order to confirm whether it shows quantitative electrochemical behaviors or not, its kinetic parameters, e.g. the symmetry factor (${\alpha}=0.28$), the exchange current density ($i_0=4.06{\mu}A/cm^2$), the capacity of the double layer ($C_d=2.11{\times}10^{-3}F$), the Michaelis constant ($K_M=2.45{\times}10^{-3}M$), and the time constant (${\tau}_B=0.077sec$) were investigated. Our experimental observations prove that the chloroprene rubber is a promising binder for the practical use of a carbon paste electrode.

• Journal of the Korean Society for information Management
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• v.34 no.1
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• pp.177-195
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• 2017

This study attempts to infer research fronts using factor graph model based on heterogeneous features. The model suggested by this study infers research fronts having documents with the potential to be cited multiple times in the future. To this end, the documents are represented by bibliographic, network, and content features. Bibliographic features contain bibliographic information such as the number of authors, the number of institutions to which the authors belong, proceedings, the number of keywords the authors provide, funds, the number of references, the number of pages, and the journal impact factor. Network features include degree centrality, betweenness, and closeness among the document network. Content features include keywords from the title and abstract using keyphrase extraction techniques. The model learns these features of a publication and infers whether the document would be an RF using sum-product algorithm and junction tree algorithm on a factor graph. We experimentally demonstrate that when predicting RFs, the FG predicted more densely connected documents than those predicted by RFs constructed using a traditional bibliometric approach. Our results also indicate that FG-predicted documents exhibit stronger degrees of centrality and betweenness among RFs.

• Proceedings of the Korean Society of Computer Information Conference
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• 2009.01a
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• pp.197-202
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• 2009

과학기술의 발달로 인한 사회 문화적 트렌드의 변화는 새로운 기회와 가능성을 제공해 주며, 정보통신기술은 통신과 방송, 통신과 콘텐츠 등 영역간의 경계를 허물며 융합을 가능하게 하고, 우리의 감성과 상상력을 자극하여 새로운 문화적 가능성을 열어주고 있다. 이러한 상황들은 방송 통신 융합이라는 이름으로 방송과 통신, TV와 PC 온라인과 오프라인 등의 모든 영역에서 다양한 노력이 진행되고 있다. 방송과 통신의 융합은 마치 역사상 신대륙의 개척 과정처럼 새로운 제품과 새로운 시장을 창출해내는 능력을 가지고 있기 때문에, 국내는 물론 세계의 모든 비즈니스 업체들은 이 기회의 땅을 향해 전력 질주하고 있다. 또한, 이에 따르는 콘텐츠의 융합 역시 괄목할만하며, 게임과 영화, 다큐멘터리와 드라마 등의 콘텐츠 간의 융합은 물론이고, 최근에는 모바일에서 영화를 제작하고, 게임과 소설 네트워크가 결합하고, 심지어는 게임 안에서 음악을 유통시키는 유통의 융합까지도 이뤄지고 있다. 이와 같은 다양한 융합의 확산은 미디어와 플랫폼의 등장뿐만 아니라 플랫폼 간 교차와 연결 및 통합이 가능한 미디어 전경(landscape)을 창출해 내고 있으며, 인터넷과 TV의 결합은 다양한 애플리케이션을 구현할 수 있는 전송 메커니즘을 서로 연결시켜 수많은 형태의 다중 플랫폼을 등장시키고 있다. 이로 인하여 방송 서비스와 인터넷 서비스가 네트워크나 전송 플랫폼의 구별 없이, 그리고 디바이스의 선택과 상관없이 활용되는 통합 플랫폼 환경이 폭 넓게 조성되고 있다. 따라서, 방송 통신 융합 환경에 적합한 다중 플랫폼 융합 콘텐츠는 사용자의 요구 및 새로운 비즈니스 모텔에 대한 요구를 만족할 수 있어야 하며, 일관된 기술로 통선 및 서비스간의 호환성을 유지하는 인터페이스의 표준화가 이루어져야한다. 방송 통신 융합 환경에 적합한 다중 플랫폼 융합 콘텐츠는 초고속 데이터 통신망을 활용하는 멀티미디어 및 IP 멀티캐스트 기능을 활용한 서비스들과 연계하여, 관련된 소재 산업들의 파급효과가 매우 크며, 관련 분야에 미치는 효과가 막대하므로, 이에 대한 적절한 육성전략을 고찰해보도록 한다.

• Journal of Korea Technology Innovation Society
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• v.20 no.1
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• pp.103-126
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• 2017

By the means of pervasiveness of ICT, ICT firms in the dynamic competition try to get the competitive advantage through the combination with the other industrial technologies and the firms in other industries also seek innovations in this way. To ICT suppliers, the application industries were completely different before but if ICT is applied to that industries once, the convergence phenomenon could occur as a part of an ICT industry. So, this research focuses the causality between ICT supplier's behavior and technological convergence according to the research question about what should ICT firms do for ICT convergence. We observed technological changes in the automotive industry through the Nasdaq listed firms' patent activities of 10-year period from the perspective of evolutionary theory. As a result, while the innovativeness of technological trials by ICT suppliers enhances the possibility of technological convergence, diversity of those can reduce the emergence of ICT convergence. This implies that ICT firms should focus specialized technologies for convergence rather than various technological options for the uncertain future.