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Isolation, Identification, and Fermentation Characteristics of Bacillus sp. with High Protease Activity from Traditional Cheonggukjang (전통 청국장으로부터 protease 분비능이 우수한 Bacillus sp. 균주의 분리 동정 및 발효 특성)

  • Ahn, Yong-Sun;Kim, Yong-Suk;Shin, Dong-Hwa
    • Korean Journal of Food Science and Technology
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    • v.38 no.1
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    • pp.82-87
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    • 2006
  • Twenty one strains strongly producing protease were isolated from Korean traditional Cheonggukjang. Eight strains selected by sensory evaluation on Cheonggukjang prepared with isolated strains were identified with based on biochemical properties a and 16S rDNA sequencing. Identified strains were Bacillus subtilis MB4, and Bacillus amyloliquefaciens A1, A2, B1, MC1, SB2, SC1, and SD1. Protease activities, important strain selection factor, were higher in Cheongjukjang prepared with B. subtilis MB4 (179.6 Unit) and B. amyloliquefaciens SB2 (201.9 Unit) than commercial traditional Cheonggukjang (97.9 Unit). Sensory evaluation revealed Cheonggukjang prepared with B. subtilis MB4 had flavor very similar to commercial traditional Cheonggukjang. Cheonggukjang prepared with B. suhtilis MB4 (0.0006 Pa s) and commercial traditional Cheonggukjang (0.0002 Pa s) revealed lower viscosities than those of Cheonggukjang prepared with B. amyloliquefaciens SB2, MC1, B1, A1, SD1, A2, and SC1 (0.006 to 0.008 Pa s at 1001/s. Results show Cheonggukjang could be prepared using single strain of B. subtilis MB4, maintaining high protease activity and very similar sensory and viscosity qualities with those of commercial traditional Cheonggukjang.

Improvement of Rheological and Functional Properties of Salmon FPC by Enzymatic Partial Hydrolysis 1. Production of Salmon FPC Hydrolysates and Their General Properties (효소적 부분 가수분해에 의한 연어 FPC(Fish Protein Concentrates)의 물성 및 기능성 개선 1. 연어 FPC의 가수분해물 제조와 일반적인 성상)

  • LEE Jong-Ho;LEE Keun-Tai;PARK Seong-Min;PARK Chan-kyu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.1
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    • pp.132-138
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    • 1998
  • To improve functional properties and enhance application of FPC in food industry, modified salmon FPC with enzyme treatment was produced and its general properties were investigated. Salmon FPC has over $84\%$ of protein and less than $0.18\%$ of lipid. Solubilities of FPC extracted with IPA and ethanol were very poor as less than $3\%$ in every pH range. In case of enzyme : substrate ratio of 1 : 100, degree of hydrolysis significantly increased until 4 hours and then slightly increased. No considerable differences were observed in general components of hydrolysates. Results of SDS-PAGE showed one unique band in each case and their molecular weight was less than 6,500. The flow properties of hydrolysates showed newtonian flow. Whiteness of hydrolysates were higher than that of salmon FPC as $5\~7$. There was no significant differences in the amount of peptide, but that of free amino acid slightly increased from 0.17 to 0.21 mg/ml.

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Effect of phase transformations on buckling behavior of subducting slab and tectonic implication (상전이가 섭입 슬랩의 좌굴에 미치는 영향과 지체구조적 암시)

  • Lee, Changyeol
    • Journal of the Geological Society of Korea
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    • v.54 no.6
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    • pp.657-675
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    • 2018
  • The apparent thickening of the subducting slab in the shallow lower mantle has been attributed to slab buckling. However, the scaling laws have not been quantitatively evaluated for the buckling behavior of the subducting slab when phase transformations are considered. Thus, two-dimensional dynamic subduction experiments are formulated to evaluate the effect of phase transformations on the buckling behavior of the subducting slab. The model calculations show that the phase transformation from olivine to wadsleyite at a depth of 410 km plays an important role in the development of slab buckling; increased slab pull due to the endothermic phase transformation accelerates slab sinking in the upper mantle and the subducting slab reaches the lower mantle in a shorter time than that of the experiments without the phase transformation. However, the phase transformation from ringwoodite to perovskite plus $magnesiow{\ddot{u}}stite$ at a depth of 660 km retards slab sinking into the lower mantle and the subducting slab tends to be accumulated in the transformation (transition) zone. Buckling analyses show that the scaling laws predict the buckling amplitude and period of the subducting slab with small relative errors even if the phase transformations are considered. The universal phenomenon of the slab buckling can explain apparent slab thickening in the shallow lower mantle and transformation zone under the subduction zones such as Java-Sunda and Northeast Japan. In addition, the buckling behavior of the subducting slab may be related to the periodic compressions and extensions in the Cretaceous Gyeongsang basin.

Characteristics of Static Shift in 3-D MT Inversion (3차원 MT 역산에서 정적효과의 특성 고찰)

  • Lee Tae Jong;Uchida Toshihiro;Sasaki Yutaka;Song Yoonho
    • Geophysics and Geophysical Exploration
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    • v.6 no.4
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    • pp.199-206
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    • 2003
  • Characteristics of the static shift are discussed by comparing the three-dimensional MT inversion with/without static shift parameterization. The galvanic distortion by small-scale shallow feature often leads severe distortion in inverted resistivity structures. The new inversion algorithm is applied to four numerical data sets contaminated by different amount of static shift. In real field data interpretations, we generally do not have any a-priori information about how much the data contains the static shift. In this study, we developed an algorithm for finding both Lagrangian multiplier for smoothness and the trade-off parameter for static shift, simultaneously in 3-D MT inversion. Applications of this inversion routine for the numerical data sets showed quite reasonable estimation of static shift parameters without any a-priori information. The inversion scheme is successfully applied to all the four data sets, even when the static shift does not obey the Gaussian distribution. Allowing the static shift parameters have non-zero degree of freedom to the inversion, we could get more accurate block resistivities as well as static shifts in the data. When inversion does not consider the static shift as inversion parameters (conventional MT inversion), the block resistivities on the surface are modified considerably to match possible static shift. The inhomogeneous blocks on the surface can generate the static shift at low frequencies. By those mechanisms, the conventional 3-D MT inversion can reconstruct the resistivity structures to some extent in the deeper parts even when moderate static shifts are in the data. As frequency increased, however, the galvanic distortion is not frequency independent any more, and thus the conventional inversion failed to fit the apparent resistivity and phase, especially when strong static shift is added. Even in such case, however, reasonable estimation of block resistivity as well as static shift parameters were obtained by 3-D MT inversion with static shift parameterization.

Improvement of Interfacial Adhesion of Plasma Treated Single Carbon Fiber Reinforced CNT-Phenolic Nanocomposites by Electrical Resistance Measurement and Wettability (젖음성 및 전기저항 측정을 이용한 플라즈마 처리된 단일 탄소섬유 강화 탄소나노튜브-페놀수지 나노복합재료의 계면접착력 향상)

  • Wang, Zuo-Jia;Kwon, Dong-Jun;Gu, Ga-Young;Park, Jong-Kyoo;Lee, Woo-Il;Park, Joung-Man
    • Journal of Adhesion and Interface
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    • v.12 no.3
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    • pp.88-93
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    • 2011
  • Optimal dispersion and fabrication conditions of carbon nanotube (CNT) embedded in phenolic resin were determined by electrical resistance measurement; and interfacial property was investigated between plasma treated carbon fiber and CNT-phenolic composites by electro-micromechanical techniques. Wettability of carbon fiber was improved significantly after plasma treatment. Surface energies of carbon fiber and CNT-phenolic nanocomposites were measured using Wilhelmy plate technique. Since surface activation of carbon fiber, the advancing contact angle decreased from $65^{\circ}$ to $28^{\circ}$ after plasma treatment. It was consistent with static contact angle results of carbon fiber. Work of adhesion between plasma treated carbon fiber and CNT-phenolic nanocomposites was higher than that without modification. The interfacial shear strength (IFSS) and apparent modulus also increased with plasma treatment of carbon fiber.

Synthesis of Poly(oxyethylene-co-adipate)-diol from Adipic Acid and Polyethylene Glycols: Effect of Catalyst Concentration (아디프산과 폴리에틸렌글리콜로부터 폴리(옥시에텔렌-아디페이트)-디올 공중합체 합성: 촉매농도의 영향)

  • Jung, Yong-Sung;Lee, Sang-Ho
    • Elastomers and Composites
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    • v.49 no.2
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    • pp.110-116
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    • 2014
  • We investigated the effect of the concentration of stannous 2-ethylhexanoate catalyst on the esterification rate between adipic acid (AA) and each of two PEG oligomers, diethylene glycol (DEG) and polyethylene glycol (PEG600). The concentration of the catalyst was varied from 0.15 to 2.0 wt.%. To attach hydroxy group to each end of the poly(oxyethylene-co-adipate) synthesized from AA and the PEGs, the esterification was performed with excessive PEG oligomers ([PEG]/[AA]=2) at $170^{\circ}C$. The degree of polymerization of the poly(oxyethylene-co-adipate)diol products were three. The apparent rate constant ($k_{app}$) of the esterification between AA and DEG shows the first order dependency on the catalyst concentration ($k_{app}=0.88[C_{cat}]$), whereas the $k_{app}$ of the esterification between AA and PEG600 has a relation of $k_{app}=0.123[C_{cat}]^{0.55}$ with the catalyst concentration. It is expected that the rate of esterification between AA and DEG has a non-linear dependency on the catalyst concentration as the catalyst concentration approaches to 0.22M.

Effect of Coal Tar Pitch Viscosity on Impregnation for Manufacture of Carbon Blocks with High Density (고밀도화 탄소 블록 제조 시 콜타르계 피치의 점도가 함침에 미치는 영향)

  • Cho, Jong Hoon;Hwang, Hye In;Kim, Ji Hong;Lee, Young-Seak;Im, Ji Sun;Kang, Seok Chang
    • Applied Chemistry for Engineering
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    • v.32 no.5
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    • pp.569-573
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    • 2021
  • In this study, high-density carbon blocks were manufactured using coke, binder pitch, and impregnated pitch, then the effect of pitch fluidity on the densification of carbon blocks during the impregnation process was investigated. A green block was manufactured through high-pressure figuration of coke and binder pitch, and a carbon block was obtained through a heat treatment process. An impregnation process was performed to remove pores generated by volatilization of the binder pitch during the heat treatment process. The impregnation process was carried out the high-pressure reaction step of impregnating the pitch into the carbon block followed by the pretreatment step of melting the impregnation pitch. Melting of the impregnation pitch was carried out at 140~200 ℃, and the viscosity of the impregnation pitch decreased as the heat treatment temperature increased. The decrease in the viscosity of the impregnation pitch improved the fluidity and effectively impregnated the pores inside the carbon block, reducing the porosity of the carbon block by 83% and increasing the apparent density by 5%.

Physicochemical Properties of Extruded Defatted Hemp Seed and Its Energy Bar Manufacturing (압출성형 삼종실의 이화학적 특성과 에너지바의 제조)

  • Gu, Bon-Jae;Norajit, Krittika;Ryu, Gi-Hyung
    • Food Engineering Progress
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    • v.14 no.2
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    • pp.127-134
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    • 2010
  • This study was to develop high-nutritious energy bar from extruded hemp obtained by extrusion process. Mixture of rice flour and defatted hemp was extruded at a barrel temperature of 110 and 130$^{\circ}C$, and moisture content of 20 and 25%. Properties of extrudates such as bulk density, expansion index, breaking strength, apparent elastic modulus, water absorption index (WAI), water solubility index (WSI) have been analyzed. The antioxidant potential was determined by the DPPH-radical scavenging assay. The expansion index was the highest in rice-hemp extrudate at 130$^{\circ}C$ barrel temperature and 20% moisture content among the other hemp-added extrudates. The WAI was increased with increase in moisture content, while the WSI was increased with increase in barrel temperature. The peak viscosity of rice extrudate had higher valule than those of extrudate added with hemp. DPPH scavenging activity of rice-hemp extrudate at 130$^{\circ}C$ barrel temperature and 20% moisture content showed the highest value. Sensory properties, moisture content and color were assessed for quality of energy bar. The color values of the energy bar indicated decreasing L (lightness) and b (yellowness), and increasing a (redness) after 30 days storage at ambient condition. The highest overall acceptable was the energy bar added with rice-hemp extrudate at 130$^{\circ}C$ barrel temperature and 20% moisture content.

A study on the behaviour of single piles to adjacent Shield TBM tunnelling by considering face pressures (막장압의 크기를 고려한 Shield TBM 터널 근접시공이 단독말뚝의 거동에 미치는 영향에 대한 연구)

  • Jeon, Young-Jin;Kim, Jeong-Sub;Jeon, Seung-Chan;Jeon, Sang-Joon;Park, Byung-Soo;Lee, Cheol-Ju
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.20 no.6
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    • pp.1003-1022
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    • 2018
  • In the current work, a series of three-dimensional finite element analyses were carried out to understand the behaviour of a pre-existing single pile to the changes of the tunnel face pressures when a shield TBM tunnel passes underneath the pile. The numerical modelling analysed the results by considering various face pressures (25~100% of the in-situ horizontal stress prior to tunnelling at the tunnel springline). In the numerical modelling, several key issues, such as the pile settlements, the axial pile forces, the shear stresses have been thoroughly analysed for different face pressures. The head settlements of the pile with the maximum face pressure decreased by about 44% compared to corresponding settlement with the minimum face pressure. Furthermore, the maximum axial force of the pile developed with the minimum face pressure. The tunnelling-induced axial pile force at the minimum face pressure was found to be about 21% larger than that with the maximum face pressure. It has been found that the ground settlements and the pile settlements are heavily affected by the face pressures. In addition, the influence of the piles and the ground was analysed by considering characteristics of the soil deformations. Also, the apparent safety factor of the piles are substantially reduced for all the analyses conducted in the current simulation, resulting in severe effects on the adjacent piles. Therefore, the behaviour of the piles, according to change the face pressures, has been extensively examined and analysed by considering the key features in great details.

Fundamental Properties and Radioactivity Shielding Characteristics of Mortar Specimen Utilizing CRT Waste Glass as Fine Aggregate (폐 브라운관(CRT) 유리를 잔골재로 대체한 모르타르 시험체의 기초 물성 및 방사선 차폐 특성)

  • Choi, Yoon-Suk;Kim, Il-Sun;Choi, So-Yeong;Yang, Eun-Ik
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.23 no.1
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    • pp.163-170
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    • 2019
  • In recent years, various types of industrial wastes are rapidly increasing with the development of high-tech industries. Specially, high-density waste glass of CRT TV containing heavy metals are buried or disposed of due to reprocessing costs and environmental pollution problems. Thus, more basic research is needed to recycle waste such as CRT waste glass such. In this study, the fundamental properties and radiation shielding performance of mortar specimens substituted CRT waste glass as a fine aggregate were analyzed and their application to shielding materials was evaluated. According to the results, the bulk density of mortar specimen replaced with CRT waste glass was increased and the compressive strength and flexural strength were decreased. Meanwhile, the CRT waste glass substitute specimen containing a large amount of lead component showed a higher shielding performance than the general mortar specimen. Especially, the linear attenuation coefficient of CRT waste glass in $122KeV{\cdot}^{57}Co$ of the low energy field was 2.5 times higher than that of normal specimen.