• KSBB Journal
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• v.15 no.2
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• pp.214-218
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• 2000

A hepatitis B Surface Antigen(HBsAg)-screening kit using immunochromatographic assay(ICA) method was developed by e employing two kinds of antibodies. One is mouse monoclonal anti-HBs for tracer antibody and the other is goat p이yclonal a anti-HBs for capture antibody. This capture antibody was immobilized on the surface of nitroceliulose(NC) membrane and the t tracer antibody was conjugated with g미d particles. When serum sample was added to the sample well, the $\infty$njugates d deposited in a dry state on the surface of glass fiber filter were reconstituted and then combined with HBsAg in serum. In 5 5 min after adding, the assay result was visible through the window, that is, the complexes composed of HBsAg and the c conjugates appeared as maroon line on the lower part of the NC membrane. The detection limit of the ICA kit was 2 ng/ml w when being tested with the reference HBsAg.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2005.05a
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• pp.207-211
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• 2005

시중의 정육점 및 백화점 등에서 유통 중인 10종의 돈육 원료에 대한 일반 세균수, 저온균수, 고온균, 혐기성균 및 진균류, 대장균군에 대한 미생물학적 분포와 병원성 미생물에 대한 분리${\cdot}$동정을 실시하였다. 실험결과 원료 돈육에서 중온균은 $3.9{\times}10^2{\sim}3.9{\times}10^5\;cfu/g$으로 높은 분포를 보였고, 저온균은 $1.5{\times}10^3{\sim}8.6{\times}10^2\;cfu/g$, 혐기성균은 중온균, 저온균과 유사한 분포를 보였으나 상대적으로 적게 검출되었고, 고온균은 모든 검체에서 검출되지 않았다. 대장균군 또한 모든 검체에 대해서 검출되지 않았으며, 곰팡이와 효모 등 진균류는 $3.8{\times}10^1{\sim}5.1{\times}10^2\;cfu/g$으로 검출되었다. B. cereus 는 돈육 sample B와 J에서 분리되었고, S. aureus의 경우 돈육 sample B에서만 검출되었다. B. cereus는 99.8%의 상동성을 보였고, S. aureus는 97.8%의 상동성을 보였다.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2009.05a
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• pp.364-368
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• 2009

자궁경부암은 다른 암과 달리 전암(前癌) 단계가 존재하므로 조기에 발견할 경우 생존율이 높다. 그러나 검체 적정성의 부족과 검체 체취의 오류로 인해 질병이 있음에도 음성으로 나타나는 위음성률이 높다. 따라서 본 논문에서는 세포 도말검사에서 사용되는 자궁 경부진 세포에서 암종 세포를 추출하는 방법을 제안한다. 영상의 배경 그리고 핵과 세포질 영역의 구분이 중요하기 때문에 조기 자궁 경부 세포진 영상에서 핵의 추출은 Lighting Compensation을 적용하여 영상을 보정하고, 명암도 분포가 가장 작은 B 채널과 명암도 분포가 높은 R채널과의 OR 연산을 적용한 후, $3{\times}3$마스크를 이용하여 잡음을 제거한다. 잡음이 제거된 영상을 이진화하고 Grassfire 알고리즘을 이용하여 암종 세포의 후보 객체를 추출한다. 추출된 세포 객체에서 핵의 크기, 핵의 면적과 핵의 외곽의 방향성 정보를 이용하여 백혈구와 잡음으로 구성된 객체를 제거한다. 세포 도말검사 과정에서 겹쳐진 부분은 거리 함수와 명암도를 이용하여 마커를 추출하고 추출된 마커 정보와 워터쉐드 알고리즘을 적용하여 겹쳐진 암종 세포를 분리한다. 자궁경부 편평 세포진 400 배율 영상과 자궁 경부 상피내 종양 400 배율 영상을 대상으로 실험한 결과, 기존의 자궁 경부진 암종 세포 추출 방법보다 효과적으로 암종 세포 영역이 추출되는 것을 확인하였다.

• Journal of Platform Technology
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• v.11 no.1
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• pp.60-71
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• 2023

The pet industry is also developing and growing in various ways as the number of companions is steadily increasing around the world and social awareness of pets has changed to members of the "family." The pet tech market, which can check health changes early by utilizing IOT technology, is also drawing attention as pets often cannot be expressed even if they have health problems. Based on this problem recognition, this study aims to promote the cold chain service market by developing important animal sample transport services in the pet healthcare market and to develop solutions that satisfy all stakeholders such as pets, pets, and animal hospitals. It was developed to apply sustainability related to society and culture as well as economic and environmental values in the research process.

• Proceedings of the Korean Society of Computer Information Conference
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• 2022.07a
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• pp.85-87
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• 2022

루푸스 신염을 정확히 진단하기 위해서는 신장의 침 생검을 통한 조직검사를 통해 사구체들을 찾아내고, 각각의 염증 정도를 분류해야 한다. 하지만 이에는 의료진의 많은 시간과 노력이 소요된다. 따라서 본 연구에서는 이러한 한계를 극복하기 위해 합성곱 신경망 (Convolutional neural network, CNN)에 기반한 검출 및 분할에 딥 러닝 접근법을 적용하는 YOLOv5 알고리즘을 통해 검체 이미지 내에서 사구체를 자동으로 검출해 내도록 하였다. 그리고 루푸스 신염 환자의 슬라이드 이미지에 대한 태깅 작업을 거쳐 학습을 위한 데이터와 테스트를 위한 데이터를 생성하여 학습 및 테스트에 활용하였다. 그 결과 고화질의 검체 이미지 내에서 대부분의 사구체를 0.9 이상의 높은 precision과 recall로 검출해 낼 수 있었다. 이를 통해 신장 내부의 사구체 검출을 자동화하고 추후 연구를 통해 사구체 염증 정도를 단계화 할 수 있는 발판을 마련하였다.

• Journal of the Korean Society of Radiology
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• v.84 no.2
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• pp.361-371
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• 2023

The success of image-guided breast biopsy depends on the biopsy method, needle selection, and appropriate technique based on the accurate judgment by the radiologist at biopsy. However, insufficient or inappropriate sampling of specimens may result in false-negative results or pathologic underestimation. Therefore, image-pathology concordance assessments after biopsy are essential for appropriate patient management. Particularly, the assessment of image-pathology concordance can avoid false-negative reports of breast cancer as a benign pathology. Therefore, this study aimed to discuss factors that impact the accurate interpretation of image-guided breast biopsy along with the appropriate assessments.

• Pediatric Infection and Vaccine
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• v.14 no.1
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• pp.91-96
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• 2007

Purpose : This study was performed to evaluate a new rapid diagnostic kit (BIOLINE $RSV^{TM}$; Standard Diagnostics Inc., Yongin, Korea), a lateral-flow immunoassay, in the detection of respiratory syncytial virus (RSV) from the nasopharyngeal aspirates (NPA) of children with lower respiratory tract infections (LRTIs) in comparison with other diagnostic methods. Methods : Three hundred and nineteen NPAs were selected from a large pool of NPAs that had been obtained from children with LRTIs. All specimens had already been tested for RSV by culture and immunofluorescent (IF) test, and had been kept frozen. Tests with BIOLINE $RSV^{TM}$ were performed at least twice. All who conducted the experiments or interpreted the test results were blinded to the results of both culture and IF tests. Results : One hundred seven (97.3%) of 110 specimens that were positive for RSV by both culture and IF test, 29 (87.9%) of 33 that were positive by IF test only, 20 (76.9%) of 26 that were positive by culture only, and 140 (93.3%) of 150 that were negative by both methods were negative for RSV by BIOLINE $RSV^{TM}$. By combining the above results, the following 5 diagnostic values of BIOLINE $RSV^{TM}$ were determined in comparison with viral culture or IF test; sensitivity, 92.3% (156/169, 95% confidence interval [CI], 87.1-97.5%); specificity, 93.3% (140/150, 95% CI, 88.4-98.2%); positive predictive value, 94.0% (156/166, 95% CI, 89.5-98.5%); negative predictive value, 91.5% (140/143, 95% CI, 86.0-97.0%); and agreement, 95.9% (306/319, 95% CI, 92.1-99.7%), respectively. Conclusion : This study revealed that BIOLINE $RSV^{TM}$ demonstrated good sensitivity and specificity for the detection of RSV antigen from NPAs of children with LRTIs. Because of simple methods and quick results, this test may be useful for the diagnosis of RSV infection during the epidemic periods.

• Korean Journal of Clinical Laboratory Science
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• v.49 no.2
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• pp.79-87
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• 2017

GenesWell$^{TM}$ BCT is a 12-gene test suggesting the prognostic risk score (BCT Score) for distant metastasis within the first 10 years in early breast cancer patients with hormone receptor-positive, HER2-negative, and pN0~1 tumors. In this study, we validated the analytical performance of GenesWell$^{TM}$ BCT. Gene expression values were measured by a one-step, real-time qPCR, using RNA extracted from FFPE specimens of early breast cancer patients. Limit of Blank, Limit of Detection, and dynamic range for each of the 12 genes were assessed by serially diluted RNA pools. The analytical precision and specificity were evaluated by three different RNA samples representing low risk group, high risk group, and near-cutoff group in accordance with their BCT Scores. GenesWell$^{TM}$ BCT could detect gene expression of each of the 12 genes from less than $1ng/{\mu}L$ of RNA. Repeatability and reproducibility across multiple testing sites resulted in 100% and 98.3% consistencies of risk classification, respectively. Moreover, it was confirmed that the potential interference substances does not affect the risk classification of the test. The findings demonstrate that GenesWell$^{TM}$ BCT have high analytical performance with over 95% consistency for risk classification.

• Korean Journal of Clinical Laboratory Science
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• v.49 no.2
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• pp.141-149
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• 2017

To examine the cause of acute diarrheal disease in children and adolescents, 521 fecal samples underwent multiple-PCR for a period of two years, between March 2015 and February 20178, in the Jeju region of Korea. Based on the analysis of 179 positive samples, 102 samples were male (56.98%) and 77 were female (43.02%), and highest positive rates were shown in the age group of 3~4 years (51.96%) and 5~6 years (12.29%). When 209 cases (40.12%), including double infection were analyzed, there were 88 cases (16.89%) of norovirus-GII infection, 26 cases (4.99%) of Campylobacter spp. infection, 18 cases (3.45%) of rotavirus infection, 18 cases (3.45%) of Clostridium difficile Toxin B infection, 17 cases (3.26%) of adenovirus infection, 16 cases (3.07%) of Clostridium perfringens infection, 11 cases (2.11%) of astrovirus infection, 5 cases (0.96%) of Salmonella spp. infection, 3 cases (0.58%) of norovirus-GI, Yersinia spp. and Aeromonas spp. infections, and 1 case (0.19%) of verocytotoxin-producing E. coli infection. Based on a seasonal separation of early childhood, norovirus-GI and norovirus-GII mainly prevailed during the winter, when the temperature is low. Typical enteritis with an increased prevalence of rotavirus during the spring. Astrovirus prevailed between the months of April and October, when norovirus-GI, norovirus-GII, and rotavirus did not prevail. With increasing age, acute diarrheal disease was not only induced by a virus, but also by bacteria. Although a test for virus is an effective method when trying to identify the cause during early childhood by multiplex-PCR, it would be desirable to undergo tests for both virus and bacteria concurrently as age increases.

• Tuberculosis and Respiratory Diseases
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• v.58 no.3
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• pp.248-256
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• 2005

Background : Nontuberculous mycobacterial (NTM) infections are increasingly being recognized as a cause of chronic pulmonary disease. This study describes the prevalence of NTM species from clinical specimens and the clinical characteristics of NTM pulmonary disease. Material and Methods : The NTM isolated from March 2003 to December 2003 at the Kosin Medical Center were identified using an oligonucleotide chip containing the internal transcribed space (ITS) sequence. The medical records of the patients with the NTM isolates, who fulfilled the 1997 ATS diagnostic criteria for NTM pulmonary disease, were analyzed, retrospectively. Results : Twenty four species (24.2%) of NTM were isolated from 99 cultured AFB specimens. M. avium complex (MAC) (13 isolates), M. szulgai (3), M. kansasii (2), M. malmoense (2), M. abscessus (1), M. chelonae (1), M. scrofulaceum (1), and unclassified (1). Of the 23 patients with isolated NTM, 11 patients were found to be compatible with a NTM pulmonary infection according to the ATS criteria; MAC was found in 6 cases (54.5%), M. szulgai in 2 cases (18.2%), and M. abscessus, M. szulgai, M. kansasii and M. malmoense in 1 case each (9.1%). Ten patients (91%) were male and the median age at diagnosis was 61 years. In the pre-existing diseases, malignant disease was found in 6 cases including 5 patients with lung cancer, and history of old pulmonary tuberculosis was identified in 4 cases. The radiological patterns showed lung destruction lung in 3 cases, a cavitary mass in 3 cases, a nodular pattern in 2 cases, and reticulonodular, consolidation and a bronchiectasis pattern were in 1 case each. Conclusion : Various types of NTM pulmonary diseases were found in a tertiary hospital at Busan, Korea. The NTM pulmonary diseases were caused by MAC, M. szugai, M. kansasii, M. malmoense, M. abscessus, M. chelonae, and M. scrofulaceum in the order of frequency.