• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.41 no.5
• /
• pp.608-618
• /
• 1996

Wild perennial types of genus Glycine, as sources of genetic diversity, have been recently employed in the soybean breeding programs. This study was carried out to introduce some useful characters of wild perennial Glycine tomentella into common cultivars, G. max. Parent materials used for interspecific hybridization were three common cultivars, Ulsankong, Baemkong and Danyeobkong, and four G. tomentella strains having different diploid(2n) of 38, 40, 78, and 80. Their cross-fertility, effect of reciprocal cross, development comparison of crossing pod and optimum timing to efficient crossing were investigated. Plant were grown in 1/2,000 Wagner pots in greenhouse and field. Crossing efficiency and pod survival rate were the highest in 2n=38 of the four G. tomentella strains when the common cultivars were fixed as female parents. In the reciprocal cross of three common cultivars and four G. tomentella strains, when the former was used as maternal sources, they showed better crossing rate and longer pod survival than the latter. In the interspecific hybridization between G. max cv. Baemkong andG. tomentella, 2n=38, the pod formed from the female G. tomentella had relatively greater size and weight than that from the female Baemkong in comparison with parents. However, the reverse result was true in a number of ovules per pod. More efficient interspecific hybridization in the field was made on August 20, when temperature and relative humidity were higher and day length was shorter, than one or two months earlier.

• Korean journal of applied entomology
• /
• v.31 no.4
• /
• pp.452-460
• /
• 1992

To establish the economical and reliable routine bioassay system for developing new insecticidal compounds, effects of leaf-dipping time, application methods, insect species and their developmental stages on susceptibilities of insects to insecticides were studied. The stable insecticidal activity appeared at the dipping time for 30-60 seconds in leaf-dipping method, and the most effective application methods were leaf-dipping method for apterous green peach aphid adults, and third instars of diamond-back moth and tobacco cutworm, whereas seedling+insect spray method for adults or third instars of brown planthoppers. For two-spotted spider mite, leaf-dipping or intact plant spray method was favorable. In the bioassay for chitin synthesis inhibitors, the inoculation of third instars of brown planthopper, diamond-back moth, tobacco cutworm and green peach aphid, and larvae of two-spotted spider mite to the young host plants treated by spray method were adequate bioassay methods.

• Journal of Life Science
• /
• v.19 no.2
• /
• pp.264-270
• /
• 2009

We investigated the effect of black soybean and doenjang with black soybean on production of cytokines including interleukin-2 (IL-2), interleukin-6 (IL-6) and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) compared with yellow soybean and doenjang with yellow soybean. We also determined inhibitory effect of two types of soybeans and doenjang on tumor metastasis produced by colon 26-M3.1 carcinoma cells. The cytokine productions of mouse splenocytes increased by the exposure of lipopolysaccharide (LPS, 1 ${\mu}g$/ml) compared to without treatment of LPS. The LPS-induced IL-2 production was highest in the black soybean methanol extract, while the methanol extract from black soybean doenjang fermented for 2 mo showed the higher levels of IL-2 without LPS (p<0.05). In case of LPS-induced IL-6 production, the methanol extracts from control, yellow soybean, black soybean doenjang fermented for 2 and 7 mo showed higher levels of IL-6 compared to those of black soybean, and yellow soybean doenjang fermented for 2 mo (p<0.05). The methanol extract of black soybean doenjang fermented for 2 mo showed significantly higher levels of TNF-${\alpha}$ in both with and without LPS (p<0.05). In experiment of tumor metastasis, the treatment (1 mg/mouse) of methanol extract from black soybean doenjang fermented for 7 mo inhibited tumor metastasis by 50% and had the highest inhibitory effect among other samples (p<0.05). From these results, doenjang manufactured with black soybean modulated the production of cytokine and showed anticancer effect, suggesting that this effect was increased with increased periods of fermentation.

• Journal of Food Hygiene and Safety
• /
• v.26 no.4
• /
• pp.410-416
• /
• 2011

Cereals are the main raw material for sunsik manufacture. As the fundamental processing step, it is very important to confirm the level of the microorganism contamination in the cereals. This study was carried out a micrbiological screening of cereal samples for sunsik from 19 companies located in South Korea. Ten kinds of cereals which were glutinous rice, barley, brownrice, blackbean, blackrice, blacksesame, sorghum, millet, perilla seed, and adlay were investigated. As the results, the contaminations of general bacterial were 3.1~8.6 log(CFU/g). The results of Escherichia coli were 1.0~3.4 log(CFU/g). There was no contamination of Salmonella. spp in any cereal samples except black sesame and mold was detected in barley. The experiment for microbiological contamination during sunsik processing was also investigated in this study. The results of general bacteria were detected as 5.1~8.5, 4.4~7.5, 1.0~2.3, 2.4~4.2, 1.0~4.0, 3.4~4.2, 4.3~5.2, and 3.3~5.5 log(CFU/g) during environment of warehousing, washing, steaming, 1st cooling, drying, 2nd cooling, grinding, and packaging process, respectively. The results of coliform were 1.0~2.0 log(CFU/g) during warehousing respectively. Mold was found in warehousing. In case of the instruments, the contaminations of general bacterial were 4.2~7.5, 0.1~2.0, 0.1~3.2, 3.7~4.0, 2.5~3.0, and 3.8~5.2 log(CFU/g) in cereals tanks, washing machines, grinding machines, packaging machines, and workrooms. The results of coliform were 2.4~4.0, 0.0~4.1 log(CFU/g) in cereals tanks and grinding machines, respectively. Mold were only found in cereals tanks, grinding machines, and workings. Therefore, the risk of hazard microorganisms contmination might be decrased as the exhaustive management is applied to the whole sunsik process.

• Korean journal of applied entomology
• /
• v.38 no.3
• /
• pp.225-230
• /
• 1999

This study was conducted to determine the effect of pupal development and adult longevity of Spodoptera litura larvae reared on leaves of different leguminos plants of 11 varieties or cultivars. Pupal duration varied from 8.7 to 9.5 days depending upon different diets and duration of female pupa (8.3~9.1 days) was about 1 day shorter than that of male's (9.4~10.1 days). Prepupal an postpupal duration were 1.7 to 2.0 days and 6.7 to 7.7 days, respectively. Pupal weight ranged from 0.23g to 0.43g for female and 0.24g to 0.35 g for male. Percent change of pupal weight was lowest (11%) on geomjeongkong-1 and highest (16%) on bukwangkong. Adult longevity was in ranging from 7.8 days on dongbu to 11.2 days on bukwangkong and tended to become slightly longer in female than tin male. Significant relationships were recognized between moisture content of feeding leaf, the pupal duration and weight and adult longevity.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.39 no.5
• /
• pp.458-464
• /
• 1994

The partitioning of dry matter into seed and pod-shell is important because yield increase can be achieved by improving the distribution rate of assimilation products to seeds. The present study was undertaken to characterize whether the partitioning of dry matter into seed was shoot- or root-controlled through graftiong techniques. Self- and reciprocal-grafts were made among four soybean genotypes, which were 'Baekunkong', 'Suwon 168', and two local soybeans with black seed coat (hereafter referred to as the 'black soybean'), 'Kangleungjarae' and 'Keumleungjarae'. Self-grafted black soybeans showed lower ratio of seed to pod-shell dry wight than self-grafted Baekunkong and Suwon 168. Varying the shoot genotypes in grafts resulted in significant differences in growth characters as well as pod and seed dry weight per plant at physiological maturity. There were significant effects of shoot genotypes on the ratio of seed to pod-shell dry weight, indication that the partitioning of dry matter into seed and pod-shell should be shoot- rather than root-controlled. The grafts with large pod-shell dry weight tended to partition relatively smaller dry matter into seed than pod-shell.

• Korean journal of applied entomology
• /
• v.28 no.1
• /
• pp.16-22
• /
• 1989

A series of experiments was undertaken to learn bionomics and gowt plant resistance of the seedcorn maggot, Delia platura(Meigen), under controlled(24$\pm$$2^{\circ}C$, RH70$\pm$5%, and LD 16:8h)and field conditions. The preoviposition period for the flies was 9 days. The females survived for an average of 50(3-77) and the males for 24(1-59) days. A greater proportion of flies emerged between 6:00 A.M. and 9:00 A.M., soon after the sun rise. After the over-wintering, adults started to emerge in mid-April from pupae located near the soil surface, and peaked in late April by others located deeper. The sex ratio was about 1:1 with total samples of 1,609 females and 1,641 males. Weight of pupae reared from onion was heavier than those from other diets in the laboratory, however its size was samller than that of natural flies. Considerably more eggs were laid near pea seeds than other hosts tested. Among beans, Bapmitkong with blue seed-coat and a cowpea bean strain were preferred for oviposition. 'Namcheon` cultivar was found to be susceptible to attack by the larvae in the laboratory.

• Korean journal of applied entomology
• /
• v.52 no.4
• /
• pp.437-448
• /
• 2013

Hemipteran bugs, which were previously considered as secondary pests, have currently become important pests of numerous crops. Among them, Riptortus pedestris (Fabricius) is a major species that occurs in Korea, Japan, China, and South Asian countries. Riptortus pedestris infests leguminous crops like soybean, vetches, and red clover; fruit trees like persimmon and yuju; and grains like barley, foxtail millet, broomcorn, and sorghum. Riptortus pedestris causes the greatest damage to soybean, as it is the most suitable host for the bug. Feeding damage during pod formation significantly reduces the yield of soybean. Currently, 17 insecticides, including diazinon and etofenprox, are registered for the control of hemipteran bugs in Korea, and growers apply insecticides two to three times on a regular basis. Aggregation pheromone traps are widely used as a monitoring tool and partial control measure. The aggregation pheromone of R. pedestris attracts conspecific adults and nymphs and is used for food exploitation rather than sexual attraction. In addition, the pheromone serves as a kairomone for egg parasitoids such as Gryon japonicum (Ashmead) and Ooencyrtus nezarae Ishii. As a new method of pest management, nonviable host eggs were included in the pheromone trap to catch R. pedestris and propagate parasitoids. As a part of cultural practices, resistant soybean varieties with specific color and size of pod and control of flowering time through the alteration of planting date can be used. For the effective management of R. pedestris in the near future, development of cultural practices that can support natural control factors and the use of multiple control tactics are needed.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.41 no.5
• /
• pp.592-599
• /
• 1996

The variations of asparagine and aspartic acid contents among the soybean varieties and in the different parts of soybean sprout after the different durations of storage were investigated. Asparagine and aspartic acid are known to have the detoxifying effects on acetaldehyde, which is highly toxic metabolites in the process of alcohol metabolism in the human body. The contents of asparagine and aspartic acid of beansprout showed continuosuly increasing trends along with the days to cultivation with a great varietal difference, especially in the roots. The duration of seed storage did not affect contents of asparagine content; the longer storage decreased aspartic acid content remarkably.

• Korean Journal of Breeding Science
• /
• v.42 no.3
• /
• pp.267-271
• /
• 2010

A new black seed coat soybean cultivar, 'Daeheug', was developed at the National Institute of Crop Science (NICS) in 2007. The breeding goal for black seed coat soybean is to develop the cultivar with large seed size, high yield, lodging tolerance, and resistance to disease such as soybean mosaic virus (SMV), and bacterial pustule. 'Daeheug' was selected from the cross between 'Daehwang' which had yellow seed coat, large seed size, and late maturity, and 'Milyang 79' which was lodging tolerant, and SMV resistant with medium seed size, black seed coat, and yellow cotyledon. The preliminary, advanced and regional yield trials for evaluation and selection of this cultivar were carried out from 2003 to 2007. It has determinate growth habit, purple flower, brown pubescence, brown pod color, black seed coat, yellow cotyledon, spherical flattened seed shape, oval leaf shape and large seed size (34.3 g/100 seeds). In maturity, 'Daeheug' was 2 days later than the check cultivar 'Ilpumgeomjeongkong'. In the seed quality such as isoflavone contents ($635{\mu}g/g$), and anthocyanin contents ($11.2m{\ell}/g$ of seed coat), 'Daeheug' was better than the check cultivar. It has good adaptability for cooking with rice in ratio of water absorption and seed hardness. Specially, it has good processability for soybean tea with 2.13 absorbance at 530 nm, scavenge activity of DPPH radical was high, and contents of total phenolics was $1,263{\mu}g/m{\ell}$. The average yield of 'Daeheug' was 2.26 ton per hectare in the regional yield trials (RYT) carried out in six locations of Korea from 2005 to 2007.