• Korean Journal of Environmental Biology
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• v.39 no.4
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• pp.415-422
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• 2021

Cotton is an important fiber crop, and its seeds are used as feed for dairy cattle. Crop biotechnology has been used to improve agronomic traits and quality in the agricultural industry. The frequent unintentional release of LM cotton into the environment in South Korea is attributed to the increased application of living modified (LM) cotton in food, feed, and processing industries. To identify and monitor the LM cotton, a method for detecting the approved LM cotton in South Korea is required. In this study, we developed a method for the simultaneous detection of four LM cotton varieties, MON757, MON88702, COT67B, and GHB811. The genetic information of each LM event was obtained from the European Commission-Joint Research Centre and Animal and Plant Quarantine Agency. We designed event-specific primers to develop a multiplex PCR method for LM cotton and confirmed the specific amplification. Using specificity assay, random reference material(RM) mixture analysis and limit of detection(LOD), we verified the accuracy and specificity of the multiplex PCR method. Our results demonstrate that the method enabled the detection of each event and validation of the specificity using other LM RMs. The efficiency of multiplex PCR was further verified using a random RM mixture. Based on the LOD, the method identified 25 ng of template DNA in a single reaction. In summary, we developed a multiplex PCR method for simultaneous detection of four LM cotton varieties, for possible application in LM volunteer analysis.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.12a
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• pp.71-71
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• 2020

산채는 사람에 의하여 개량 육성되어 논밭에서 재배하고 있는 농작물과 달리 자연 그대로 산야에서 자생하는 식물 중 식용으로 가능한 것을 말한다. 산채류의 이용형태별 생산가능시기는 3~5월의 생채 생산과 6~7월의 건채 생산으로 소비자의 기호에 부합하는 신선 생채의 소비한계는 제한되어 있기 때문에 재배유형별 생채 수량검정을 통해 수확시기 연장 및 재배품목을 다양화 시킬 필요 있다. 따라서 본 시험은 재배가능한 산채류를 수집하고 기능성 분류를 통한 꾸러미 산채 조합을 구성하였고 조합된 산채류 들에 대한 생산성 검정을 위해 해발 500m의 전라북도 농업기술원 허브산채시험장에서 2018년 5월 말 갯기름나물 등 15 종을 정식하여 각 작물의 생육특성, 수확한계기 등을 조사하였다. 기능성 분류 결과 항당뇨, 항고지혈증, 항비만, 항혈전, 항염증 총 5가지 조합을 구성하였다. 산채류별 광합성특성 조사결과 돌단풍, 개미취, 섬쑥부쟁이, 질경이, 갯기름나물, 참취의 적정광량은 600~800 μmole/m²/s이였고 눈개승마, 어수리, 참당귀는 400~500 μmole/m²/s, 곤달비, 곰취, 단풍취는 200~300 μmole/m²/s이였다. 기능성 꾸러미 산채류 지상부 생육특성 결과 갯기름나물, 질경이, 참취, 곤달비 섬쑥부쟁이 등이 생육이 우수하였다. 수량성은 섬쑥부쟁이 1,694.6kg/10a로 가장 높았으며, 갯기름나물 1,673.4kg/10a, 참취 1,521.3kg/10a, 질경이 1,398.3kg/10a, 곤달비 1,300.6kg/10a 등이 높았다. 반면 돌단풍 111.8kg/10a, 단풍취 69.5kg/10a, 우산나물 25.4kg/10a로 아주 낮은 수량성을 보였다. 산채류 출현기는 산마늘이 2월 7일로 가장 빨랐으며 대부분 산채류들이 2월 11일에서 2월 25일에 출현하였고 단풍취, 우산나물이 3월 6일로 늦은 출현기를 보였다. 기능성 꾸러미 생채 수확가능 시기는 3월 상순부터 6월 하순까지 가능하였고 수확기간은 섬쑥부쟁이 117일, 두메부추 109일, 어수리 102일, 참당귀 102일로 길었고 돌단풍, 산마늘, 눈개승마, 우산나물은 생채 수확가능 시기가 짧았다. 건나물 수확기간은 돌단풍, 눈개승마는 3월부터 6월까지 가능하였고, 대부분의 산채류들이 5월부터 10월까지고 산마늘과 우산나물은 건나물을 생산할 수 없었다. 산채류 기능성 꾸러미별 생채 조합 가능 시기는 항당뇨는 3월 중하순, 항고지혈증은 3월 중순, 항비만은 3월 하순, 항혈전 3월 하순, 항염증은 3월 중하순으로 조사되었다.

• Korean Security Journal
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• no.62
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• pp.87-111
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• 2020

Special security guards working at nuclear power plants, one of the country's major facilities, serve as human protection to safeguard from threats to nuclear facilities and nuclear materials. The purpose of this study was to develop a physical fitness program for fitness management that is essential for the completion of missions of special guards. This program was designed to prepare the physical fitness test proposed by Jeong et al. (2019). Researchers conducted literature analysis, research meetings, expert meetings and pretests, and developed a 90-minute physical fitness program for 6 weeks, 3 times a week. In order to verify the effectiveness of the developed physical fitness program, the experiment was conducted on 29 subjects(control group: 15, exercise group:14). Specifically, a six-week physical fitness program was conducted for exercise groups, and the fitness test for a special security guard was conducted for all subjects before and after the experiment. As a result, it was found that the physical fitness program was effective in improving the performance of 20m shuttle run, leg tuck, 20m sprint & carry, and medicine ball back throw. Until recently, problems of neglecting fitness management of security guards have been pointed out. It is expected that the physical fitness program proposed by this study will be a practical alternative for security guards' fitness management.

• Korean Journal of Breeding Science
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• v.42 no.6
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• pp.668-673
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• 2010

A new black seed coat soybean cultivar, 'Heugseong' was developed at the National Institute of Crop Science (NICS) in 2008. The goal to breed the black seed coat soybean is to develop the cultivar with large seed size, high yield, lodging tolerance, and resistance to disease such as soybean mosaic virus (SMV), and bacterial pustule. 'Heugseong' was selected from the cross between 'Gnome85', showing yellow seed coat, lodging tolerance, and high yield, and 'Cheongja', showing green cotyledon, and black seed coat. The preliminary, advanced and regional yield trials for this cultivar were carried out from 2004 to 2008. It has determinate growth habit, purple flower, brown pubescence, brown pod color, black seed coat, yellow cotyledon, elongated seed shape, oval leaf shape and large seed size (29.2 grams per 100 seeds). It was 4 days later in maturity than the check cultivar 'Ilpumgeomjeongkong'. 'Heugseong' was better than the check cultivar in the seed quality of isoflavone contents(1,913 ${\mu}g/g$). It has good adaptability for cooking with rice in ratio of water absorption and seed hardness, for physics of black tofu, and for fermented black soybean paste. The average yield of 'Heugseong' was 2.37 ton per hectare in the regional yield trials carried out in six locations of Korea from 2006 to 2008.

• Proceedings of the Korean Society of Crop Science Conference
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• 2007.11a
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• pp.236.1-236.1
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• 2007

• Digital Contents
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• no.6 s.157
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• pp.58-59
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• 2006

전사아키텍처가 IT 전략의 핵심 요소로 자리 잡으면서 이를 책임지고 수행할 수 있는 전문가 수요가 증가하고있다. 특히 전사아키텍처중 기업의 모든 업무를 데이터측면에서 체계화하는 데이터 아키텍처전문가의 필요성이 계속 높아지고 있는 상황이다. 이런 가운데 한국데이터베이스진흥센터가 주관하는 데이터아키텍처 전문가(Data Architecture Professional) 자격검정시험은 데이터아키텍처전문능력을 평가받고 싶어하는 사람들이 관심을 가질만한 시험으로 국내에서는 지난 3월 처음 실시됐다.

• Research in Plant Disease
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• v.16 no.3
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• pp.279-284
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• 2010

Clubroot caused by Plasmodiophora brassicae is a widespread disease that causes serious problems in many brassica growing areas. To establish more simple and reliable clubroot screening method of Chinese cabbage to P. brassicae using soil-drenching inoculation, the development of clubroot on Chinese cabbage according to several conditions such as soil type, inoculum concentration of P. brassicae GN-1 (race 9), plant growth stage and incubation period was studied. In a commercial horticulture nursery media soil (CNS), disease severity of the seedling according to inoculum concentration increased in a dose-dependent manner, but did not in mixture of CNS and upland soil (1:1, v/v). To facilitate and acquire precise result of resistance screening of Chinese cabbage to clubroot, 10-day-old seedlings should be inoculated by drenching the spore suspension of P. brassicae to give inoculum density of $4.0{\times}10^8$ spores/pot. To develop the disease, the inoculated seedlings were incubated in a growth chamber at $20^{\circ}C$ for 3 days, and then cultivated in a greenhouse ($25{\pm}5^{\circ}C$) for five weeks. Under the optimum conditions, 25 clubroot-resistant (CR) and 3 clubroot-susceptible (CS) cultivars were tested for resistance to P. brassicae. All CR cultivars showed very clear resistance response, on the other hand all CS cultivars severly infected with the pathogen. The results suggest that this method is efficient screening method of Chinese cabbage for resistance to clubroot disease.

• Research in Plant Disease
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• v.18 no.2
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• pp.86-92
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• 2012

Clubroot caused by Plasmodiophora brassicae Woron. is one of the most important diseases in Brassica crops worldwide. To establish more simple and reliable screening method for resistant cabbage and broccoli to P. brassicae, the development of clubroot on the plants according to inoculum concentration and incubation period after inoculating with the pathogen was investigated using P. brassicae GN1 isolate (race 9). To facilitate and acquire precise result of resistance screening of cabbage and broccoli to clubroot, 14-day-old seedlings were inoculated by drenching roots with the spore suspension of P. brassicae to give inoculum density of $2.5{\times}10^9$ spores/pot. To develop the disease, the inoculated seedlings were incubated in a growth chamber at $20^{\circ}C$ for 3 days, and then cultivated in a greenhouse ($20{\pm}5^{\circ}C$) for five weeks. Under the optimum conditions, 16 cabbage and 17 broccoli cultivars were tested for resistance to four field isolates (GN1, GN2, GS and YC) of P. brassicae collected from four regions in Korea. Among them, some cabbage and broccoli cultivars showed different resistance response to three isolates (GN1, GN2 and GS) determined as race 9 by using the differential varieties of Williams. On the other hand, all the tested cultivars were highly susceptible to YC isolate (race 2). The results suggest that this method is efficient screening method of cabbage and broccoli for resistance to P. brassicae.

• The Journal of the Convergence on Culture Technology
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• v.9 no.3
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• pp.281-286
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• 2023

This study analyzed the causal relationship between housing sales prices and jeonse prices in Seoul, specifically in the Gangnam and Gangbuk neighborhoods. The time-invariant Granger causality test showed bidirectional causality between the sales price and the jeonse price in Seoul and Gangbuk, but no bidirectional causality was found in Gangnam. However, the time-varying Granger causality test showed a Granger causal relationship between the housing jeonse price and the sales price for the entire period after 1993 in all three areas. Notably, the causal effect of jeonse prices on sales prices has been continuous in Gangnam since 2010. These analysis results suggest that an increase in liquidity supply to the jeonse market could increase volatility throughout the housing market, given the strong influence between the sales and jeonse markets in both directions.

• Korean Security Journal
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• no.59
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• pp.71-90
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• 2019

As the demand for national security and national safety is gradually increasing in accordance with the trend of the times and social environment, and the use of private areas for effective prevention and supply required for the demand for safety services, this paper analyzed the Japanese private security testing system for the introduction of the Korea security certification system as a way to secure expertise in the private security industry and drew the following conclusion. First, certificate segmentation should be carried out according to the clearly divided field and scope of the expense operation. Second, it is necessary to distinguish the qualification of the private security qualification according to the level. Third, it is necessary to utilize the combined evaluation method through the departmental and practical tests. Fourth, an assessment should be made through the link between departments and practical subjects. Fifth, the diversity of the acquisition methods should be placed to ensure the gainer's accessibility. Finally, the use of professional and visible use of professional personnel should be achieved through benefit assessment for those who are qualified for