• Title/Summary/Keyword: 검경

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Isolation and Properties of Cucumber Mosaic Virus Inducing Mosaic Symptoms in Hippeastrum hybridum Hort (아마리리스에 모자익병을 일으키는 CMV에 관한 연구)

  • Kim J.S.;Kim H.B.;Lee S.H.
    • Korean journal of applied entomology
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    • v.20 no.2 s.47
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    • pp.76-82
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    • 1981
  • Cucumber Mosaic Virus (CMV) was isolated from naturally infected Hippenstrum hybridum. The virus caused mosaic symptoms on Nicotiana glutinosa and local lesions on Vignaunguiculata. The thermal inactivation point was 56C, dilution end point $10^{-3}$ and longevity in vitro was 2 days for CMV from Hippeastrum. Purified virus was obtained using citrate chloroform extraction procedure and polyethylene glycol precipitation followed by sucrose density gradient centrifugation. Purified virus had a typical absorption at 245nm. Electron micrographs of the purified virus from Hippeastrum showed spherical particles with 30nm in diameter. The purified virus reacted with CMV antiserum in agar gel double diffusion test.

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The injured conditions of Eriophyes kuko $K_{ISHIDA}$ (구기자 혹응애(Eriophyes kuko $K_{ISHIDA}$의 가해상태)

  • Kim C. H.
    • Korean journal of applied entomology
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    • v.4
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    • pp.65-66
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    • 1965
  • This study was intended to know the histological damage of the gall on the leaves, peduncles and calyx of Lycium chinense $M_{ILL}$ formed Eriophyes kuko $K_{ISHIDA}$ during 1962 to 1965. The results are summarized as follows; 1. Eriophyes kuko $K_{ISHIDA}$ fed on only the Palisade layers of the loaves of Lycium chinense $M_{ILL}$ were injured by boring. 2. From the 1st to 10th day, some parts of the spongy layer of the Lycium chinense $M_{ILL}$ leaves were injured, but variation were not significant in this period. 3. From the 15th day, Eriophyes kuko $K_{ISHIDA}$ injured not only the spongy layer of Lycium chinense $M_{ILL}$. leaves but also the great part of e chloroplast of the loaves, and the sponginess caves were appeared inside of the galls.

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Investigations on the Virus Diseases in Spinach (Spinacia oleracea L.) II. Identification of Broad Bean Wilt Virus Occuring Spinach (시금치 바이러스병에 관한 연구 II. 시금치에 발생하는 Broad Bean Wilt Virus (BBWV)의 분류동정)

  • Lee S. H.;Lee K. W.;Chung B. J.
    • Korean journal of applied entomology
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    • v.18 no.1 s.38
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    • pp.11-14
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    • 1979
  • Spinaches showing dark green mosaic symptoms were used for identification of broad bean wilt virus. In host reaction test, that virus caused local lesions on the inoculated leaves and mosaic symptoms on upper leaves of Chenopodium amaranticolor, Chenopodium quinoa and Vicia faba, and developed mosaic symptoms on Physalis floridana, Spinacia oleracea, Nicotiana tabacum, (White burley, Bright yellow) Nicotiana glutinusa. In agar gel-diffusion test, the virus showed positive reaction with broad bean wilt virus antiserum. Spherical virus particles with size of 25nm in diameter were observed in electron microscope.

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Identification of Snellantchasmus falcatus Metacercariae Encysted in Mullets in Korea (남해만 숭어(崇漁)에서 검출된 Stellantchusmus fulcutus 피낭유충의 동정)

  • 채종일;손운목
    • Parasites, Hosts and Diseases
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    • v.26 no.1
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    • pp.65-68
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    • 1988
  • A group of metacercariae encysted in the Resh of Mugil sp. were identified to be Stellantchasmus falcatus by the morphology o( adult worms obtained experimentally. It is confirmed that mullets serve as a second intermediate host of this heterophyid cuke in Korea.

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Two Unrecorded Species of Tortricidae (Lepidoptera) from North Korea, with Notes on Collection Localities of North Korean Tortricids Deposited at the Hungarian Natural History Museum (북한산(北韓産) 잎말이나방과(科) (나비목(目))의 2미기록종 및 새로운 채집지 보고)

  • Byun, Bong-Kyu;Lee, Bong-Woo;Bae, Kwan-Ho;Choi, Jun-Keun
    • Korean journal of applied entomology
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    • v.52 no.1
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    • pp.53-59
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    • 2013
  • Based on the specimens of Tortricidae collected from North Korea by Hungarian Natural History Museum, totally twelve species were identified. Among them, two species, Notocelia rosaecolana Doubleday and Rhopobota ustomaculana Curtis, are reported for the first time from North Korea. All identified species is enumerated with their available information including the collecting localities, the distributional ranges and host plants.

Epidemiological Studies of Rice Blast Disease Caused by Pyricularia oryzae Cavara I. Measurement of the Amount of Spores Released from a Single Lesion (벼 도열병의 역학적 연구 I. 단일병반으로부터 포자이탈량 조사)

  • Kim Chang Kyu;Yoshino Reiichi
    • Korean Journal Plant Pathology
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    • v.3 no.2
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    • pp.120-123
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    • 1987
  • Four types of spore trap (Kim's original, improved Kim's original, Yoshino's original and mixed type of Kim's and Yoshino's original) were evaluated for their efficacy to "estimate the amount of spores released from leaf blast lesions under the natural conditions. It was found that all four types had one or two defects in allowance for adequate sporulation/release, spore catch or spore counting. Thus, an improved type of spore trap was devised considering that it could cover the defects mentioned above. As a result, newly developed spore trap was quite satisfactory in above mentioned aspects and it could be used for pursuit of spore release phase under the natural conditions.

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Effect and Histopatbologic Examination of Calcium Channel Blocker on Carbon Tetrachloride-induced Hepatotoxicity in Rats (Calcium Channel Blocker가 사염화탄소에 의한 간손상에 미치는 영향 및 조직학적 검경)

  • 전명애;정춘식;정기화
    • Biomolecules & Therapeutics
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    • v.6 no.3
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    • pp.247-255
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    • 1998
  • Carbon tetrachloride (CCI$_4$) induces the hepatotoxicity due to the reactive free radicals generated by cytochrome P-450 (CYP-450) enzyme, which result in destabilization of cellular membrane. Diltiazem, a calcium channel blocking agent, has been known to suppress the CYP-450 enzyme activities. To study the effect of diltiazem in $CCl_4$-treated rats, we measured the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and glutathione S-transferase (GST), contents of bilirubin, albumin, total protein, cholesterol, triglyceride, blood urea nitrogen, creatinine malondialdehyde and calcium. Also we conducted liver histopathologic examinations. Diltiazem, when administered 1 hour prior to CCI$_4$ treaeent, significantly reduced the activities of ALT and AST, the contents of microsomal malondialdehyde and calcium in liver and microsome as compared with those of $CCl_4$-treated rats. In addition, histopathologic examination showed that diltiazem prevented the development of centrilobular necrosis induced by CCI$_4$ in liver tissue. Our results suggested that diltiazem could inhibit the formation of free radicals and the influx of calcium. Therefore diltiazem may be applied to suppress the liver damage caused by $CCl_4$.

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Severity of Cowpea mosaic virus and Putkong Disease Monitoring and Purification of Cowpea mosaic virus (석량풋콩재배지에서의 동부모자이크바이러스병의 발생도 및 풋콩병해모니터링과 동부모자이크 바이러스의 순화)

  • Cho, Eui-Kyoo
    • Research in Plant Disease
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    • v.13 no.1
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    • pp.30-33
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    • 2007
  • One hundred and eighty-six leaves of soybean cv. Seokryangputkong that showed mild mosaic symptoms were collected randomly and ELISA tests were conducted with those leaf samples to screen the presence of Cowpea mosaic virus (CPMV). Ninety-three out of 186 samples reacted positively to CPMV, but those samples did negatively to Soybean mosaic virus (SMV). At least, 55 leaf samples revealed higher values than that of positive control. The results strongly confirmed that CPMV occurred severely in soybean cv. Seokryangputkong. However, a question is raised on the primary reservoir and vector for transmission of this virus. Since the farmer changes seeds every year, seed transmission is excluded. The virus was also purified, the analysis of coat protein conformed the virus of cowpea mosaic virus and UV absorption pattern confirmed that the causal virus of mosaic disease in soybean putkong was cowpea mosaic virus.

Growth and Yield of Azukibean Seed from Virus-infected Plant (바이러스 나병주로부터 얻은 팥의 생육 및 수량)

  • 허남기;김기식;변학수;하건수;최장경
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.5
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    • pp.569-573
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    • 1995
  • This experiment was carried out to investigate the effect of Azukibean seeds gained from virus-infected plant on the growth and yield of the next generation. In conventional cultivation, plants showed high infection rate, whereas plants in isolation cultivation showed normal growth without virus-infection. It was considered that virus-infection of Azukibean(variety Chungjupat) occurred not because of seed infection but because of insects-vector in growing period. And thread-shaped virus particles were observed in diseased leaf showing mosaic through electron microscope.

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Microstructural and Melting Characteristics of Imitation Cheese Analog (모방치즈의 조직과 융점특성 연구)

  • Song, Jae-Chul;Park, Hyun-Jeong
    • Korean Journal of Food Science and Technology
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    • v.18 no.1
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    • pp.11-15
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    • 1986
  • The imitation processed cheese (IPC), formulated with delactosed non fat dry milk (DENFDM) only, showed the smallest melting area. Calcium caseinate sample showed the largest spread. Satistically protein source as a major ingredient for the IPC yielded significantly different melting areas. In a similar fashion, initial melting temperature was markedly and significantly influenced by protein source. In effect of addition of DENFDM on microstructure of IPC analog revealed that as the fraction of DENFDM was decreased, the network was much more uniform and the fat globules were also better dispersed compared to DENFDM cheese analog. Therefore the results of this study help predict that melting and microstructural characteristics are largely but not solely dependent on the protein source. The DENFDM has a potential beneficial effect as a partial replacement of caseinate in the formation of IPC to characteristic close to processed cheese.

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