• Title/Summary/Keyword: 건조질량

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Antibacterial Activity of Yeast Transformed with Leucocin A (Leucocin A로 형질전환된 효모의 항균 활성도)

  • 이성일;이동근;이진옥;심두희;주치언;김옥수;이상현;이재화
    • KSBB Journal
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    • v.19 no.4
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    • pp.291-294
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    • 2004
  • The aim of this study was to figure out the antibacterial pattern of leucocin A transformed yeast with culture. Dry cell weight, total secreted protein, and antibacterial activity were increased to 12 hour, after then they showed decrease while protease activity represented the opposite pattern. This implied the production of leucocin A was growth-related. Compared to the result of one hour culture broth, antibacterial activity was about 3.24 fold at 12 hour culture. Maximum growth inhibition rate was 70.57% compared to nontransformed yeast. As the increase of protease in the supernatant, the antibacterial activity was diminished. This study could permit the mass production of bacteriocin to use as antibiotics or food preservatives.

Practical Application of the Active Vibration Control System to Ship Superstructure (선체 상부구조 진동의 능동제어시스템 실용화 연구)

  • T.Y. Chung;S.J. Moon;H.C. Park;Y.C. Huh;S.Y. Han
    • Journal of the Society of Naval Architects of Korea
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    • v.33 no.2
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    • pp.119-126
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    • 1996
  • A hydraulic type active control system has been developed for the purpose of vibration suppression of ship superstructrures. The active mass of the developed system weighs about three tons and is designed to have ${\pm}5mm$ stroke below 10Hz. Thus maximum control force of the system is about 60kN. Performance test of the developed system has been carried out on a 4,400TEU contrainer carrier built in Samsung Heavy Industry Co., LTD. Through the exciter tests, it has been confirmed that the vibration levels of the superstructure can be reduced to about 20% of the uncontrolled levels near resonant frequency.

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대두 단백 효소 가수분해물의 항균활성

  • 주정현;이상덕;이규희;이기택;오만진
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • 2003.04a
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    • pp.145.1-145
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    • 2003
  • 대두 단백질을 효소로 가수분해 하였을 때 생성되는 peptide의 항균활성을 조사하고 천연항균제로서 이용 가능성을 검토하기 위하여 분리 대두 단백질에 5종의 단백질 가수분해 효소를 작용시켜 생성된 가수분해물의 항균력을 측정하고 한외여과하여 분자량별로 분리된 각 fraction의 항균활성과 HPLC로 정제하여 항균성 peptide 의 아미노산 결합순서를 분석하여 다음과 같은 결론을 얻었다. 분리대두 단백질에 5종의 단백질 분해효소를 작용시켜 제조한 가수분해물 중 Asp.saitoi protease로 작용시킨 것이 항균활성이 높았다. Asp. saitoi protease로 작용시킨 대두 단백질의 가수분해물을 membrane filter로 여과한 결과 분자량 1000-3000 fraction에서 항균활성이 가장 높았다. 분자량 1000-3000 범위을 가진 가수분해물의 MIC는 0.5-0.8mg/$m\ell$ 이었으며 그람 양성균과 음성균 모두의 증식을 억제하는 경향을 보였다. 분리 대두 단백으로부터 얻어진 항균성 peptide는 121$^{\circ}C$, 10분간 열처리하여도 안정하였으며 한외여과에 의하여 분자량 1000-3000범위의 가수분해물을 동결건조하여 gel filteration하였을 때 2개의 fraction에서 항균 활성을 나타내었다. HPLC결과 RT 16.02 의 peak에서 항균활성이 확인되었고 질량은 1,633이었으며 아미노산 결합순서는 H$_2$N-G-P-P-G-V-V-A-T-V-V-A-A-R-COOH 이었다.

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Au 전극을 적용한 방사선 영상 검출기의 전기적 특성 평가

  • Song, Yong-Geun;O, Gyeong-Min;Kim, Seong-Heon;Kim, Ji-Na;No, Seong-Jin;Heo, Seung-Uk;Nam, Sang-Hui
    • Proceedings of the Korean Vacuum Society Conference
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    • 2012.02a
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    • pp.365-365
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    • 2012
  • 본 연구에서는 X-ray 영상을 얻기 위한 검출기 중 직접 검출방식에 쓰이고 있는 광도전체(Photoconductor)의 전극으로 Au를 사용하여 전기적인 특성을 파악하였다. Au는 유기물에 대한 반응이 적고 전기 전도도가 좋은 물질로서 투명전극으로 많이 쓰이고 있는 인듐 주석 산화물(ITO)을 대체할 수 있는 물질로 각광받고 있다. 우선 시편 제작을 위해 투명한 기판(Corning Glass, 0.7t)위에 하부전극으로 Au를 $3cm{\times}3cm$의 크기로 Physical Vapor Deposition(PVD) 방식을 이용하여 증착하였다. 이 때 챔버 내 저진공은 Rotary Pump를 이용하여 $3.9{\times}10-2Torr$ 이하를 유지하고 고진공은 Diffusion Pump를 이용하여 $5.3{\times}10-5Torr$ 이하를 유지하였다. 완성된 하부전극 위에 광도전체인 $HgI_2$를 폴리머 물질에 교반하여 메탈 폴리머 결합을 가진 Paste를 제조하고 이 Paste를 Screen Printing Method를 이용하여 증착 후 건조하였다. 마지막으로 시편위에 상부전극을 하부전극과 같은 조건으로 증착함으로서 시편을 완성한다. 상하부 전극으로 쓰이는 Au의 증착 조건을 변화시키면서 그에 따른 시편의 전기적인 특성 변화를 관찰하였다. 그 결과, Au의 증착온도와 질량에 따라 특성이 변화함을 알 수 있었다. 본 연구의 결과를 통해 디지털 방사선 검출기에서 Au 전극의 적용 가능성을 확인하였으며, 추후 Au 증착 조건의 최적화를 통해 방사선 검출기의 효율 향상을 위한 연구를 하고자 한다.

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Strength Characteristics of Mortar with Diatomite Powder as an Admixture (혼화재료로서 규조토 분말을 사용한 모르타르의 강도 특성)

  • Choi, Jaejin;Park, Hongtae;Kim, Jaewoo
    • Journal of the Society of Disaster Information
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    • v.11 no.3
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    • pp.329-336
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    • 2015
  • When diatomite powder was used as an admixture in mortar, its effects on the mortar strength was examined by experimental tests. For the tests, 4 kinds of commercially available diatomite powder were purchased ; one non-calcined product, one calcined product, and flux-calcined product two. The compressive and flexural strength of the mortar according to the increase of added amount of calcined diatomite powder increased at all test ages(7, 28, and 56 days). However, the use of non-calcined diatomite powder worsened the fluidity of mortar severely and that caused much more required water content. And flux-calcined product did not show useful effect on the mortar strength.

Carbon Dioxide Fixation and Light Source Effects of Spirulina platensis NIES 39 for LED Photobioreactor Design (Spirulina platensis NIES 39를 이용한 LED 광생물반응기에서의 이산화탄소 고정화와 광원 효과)

  • Kim, Ji-Youn;Joo, Hyun;Lee, Jae-Hwa
    • Applied Chemistry for Engineering
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    • v.22 no.3
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    • pp.301-307
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    • 2011
  • Optimal culture conditions of Spirulina platensis NIES 39 have been established using different types of light sources. Several types of photobioreactors were designed and the increase of biomass, the amount of $CO_2$, fixation and the production of chlorophyll content were studied. The result revealed that the input conditions of a 10 min period per 4 h at the condition of 5% $CO_2$ and 0.1 vvm, were excellent in the growth. The growth showing the maximum biomass accumulation is limited to 1.411 g/L when using the fluorescent bulb and the low powered surface mount device (SMD) type LEDs which were equipped-inside in the photobioreactor. However, the biomass exceeded up to 1.758 g/L level when a high powered red LED (color temperature : 12000 K) photobioreactor system was used. The $CO_2$ fixation speed and rate were increased. Although the total production of chlorophyll content undergoes a proportional increase in the biomass, the net content per dry cell weight (DCW) showed the higher production with a blue LED (color temperature : 7500 K) light than that of any other wavelengths. The carbon dioxide loss was marked as 0.15% of the inlet gas (5% $CO_2/Air$, v/v) at the maximum biomass culture condition.

Review of Remote Sensing Studies on Groundwater Resources (원격탐사의 지하수 수자원 적용 사례 고찰)

  • Lee, Jeongho
    • Korean Journal of Remote Sensing
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    • v.33 no.5_3
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    • pp.855-866
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    • 2017
  • Several research cases using remote sensing methods to analyze changes of storage and dynamics of groundwater aquifer were reviewed in this paper. The status of groundwater storage, in an area with regional scale, could be qualitatively inferred from geological feature, surface water altimetry and topography, distribution of vegetation, and difference between precipitation and evapotranspiration. These qualitative indicators could be measured by geological lineament analysis, airborne magnetic survey, DEM analysis, LAI and NDVI calculation, and surface energy balance modeling. It is certain that GRACE and InSAR have received remarkable attentions as direct utilization from satellite data for quantification of groundwater storage and dynamics. GRACE, composed of twin satellites having acceleration sensors, could detect global or regional microgravity changes and transform them into mass changes of water on surface and inside of the Earth. Numerous studies in terms of groundwater storage using GRACE sensor data were performed with several merits such that (1) there is no requirement of sensor data, (2) auxiliary data for quantification of groundwater can be entirely obtained from another satellite sensors, and (3) algorithms for processing measured data have continuously progressed from designated data management center. The limitations of GRACE for groundwater storage measurement could be defined as follows: (1) In an area with small scale, mass change quantification of groundwater might be inaccurate due to detection limit of the acceleration sensor, and (2) the results would be overestimated in case of combination between sensor and field survey data. InSAR can quantify the dynamic characteristics of aquifer by measuring vertical micro displacement, using linear proportional relation between groundwater head and vertical surface movement. However, InSAR data might now constrain their application to arid or semi-arid area whose land cover appear to be simple, and are hard to apply to the area with the anticipation of loss of coherence with surface. Development of GRACE and InSAR sensor data preprocessing algorithms optimized to topography, geology, and natural conditions of Korea should be prioritized to regionally quantify the mass change and dynamics of the groundwater resources of Korea.

Comparison of Solidification Pre-treatment Methods for the Determination of δ13C of Dissolved Organic Carbon: Alkaline Persulfate Oxidation-Carbonate Precipitation vs. Freeze Drying (용존유기탄소의 δ13C : 분석시 고형화 전처리 방법 비교 알칼린 과황산칼륨산화 탄산침전과 동결건조)

  • Jeon, Byeong-Jun;Park, Hyun-Jin;Choi, Woo-Jung;Park, Yong-Se;Lee, Sang-Mo;Yoon, Kwang-Sik
    • Korean Journal of Environmental Agriculture
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    • v.36 no.2
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    • pp.113-118
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    • 2017
  • BACKGROUND: The carbon (C) isotope ratio (${\delta}^{13}C$) of dissolved organic C (DOC) is an indicator of water pollution source. In this study, the potential use of two pre-treatments for the ${\delta}^{13}C$ analysis, alkaline persulfate oxidation coupled with carbonate precipitation (precipitation) and freeze drying (drying), were compared to suggest a more feasible pre-treatment method. METHODS AND RESULTS: Two reference materials with different ${\delta}^{13}C$ values were used for the experiments; chemical grade glucose ($-12.0{\pm}0.02$‰) and pig manure compost extract ($-23.3{\pm}0.04$‰). In the precipitation method, the measured ${\delta}^{13}C$ values were consistently lower than the theoretically calculated values as dissolved $CO_2$ could not be removed due to the alkaline property of the reagents and the dissolution of air $CO_2$ into the alkaline solution. The drying method also resulted in more negative ${\delta}^{13}C$ than the calculated ${\delta}^{13}C$; however, the difference was systematic ($3.9{\pm}0.3$‰) and there was a strong correlation (${\delta}^{13}C_{calculated}=0.87{\times}{\delta}^{13}C_{measured}-0.624$, $r^2=0.98$) between the calculated and measured ${\delta}^{13}C$. Calibration of ${\delta}^{13}C$ using the relationship between the calculated and the measured ${\delta}^{13}C$ values produced reliable and accurate ${\delta}^{13}C$ values. CONCLUSION: Our results suggest that the drying method is more accurate pre-treatment method to minimize the influence of air $CO_2$ compared to the precipitation method for the determination of ${\delta}^{13}C$ of DOC.

An Analysis of MODIS Aerosol Optical Properties and Ground-based Mass Concentrations in Central Korea in 2009 (2009년 한국 중부 지역에서 MODIS 에어로졸 광학 성질과 질량 농도의 분석)

  • Kim, Hak-Sung;Kim, Ji-Min;Sohn, Jung-Joo
    • Journal of the Korean earth science society
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    • v.33 no.3
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    • pp.269-279
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    • 2012
  • Satellite-retrieved data on Aerosol Optical Depth (AOD) and ${\AA}$ngstr$\ddot{o}$m exponent (AE) using a Moderate Resolution Imaging Spectrometer (MODIS) were used to analyze large-scale distributions of atmospheric aerosols in East Asia. AOD was relatively high in March ($0.44{\pm}0.25$) and low in September ($0.24{\pm}0.21$) in the East Asian region in 2009. Sandstorms originating from the deserts and dry areas in Northern China and Mongolia were transported on a massive scale during the springtime, thus contributing to the high AOD in East Asia. Although $PM_{10}$ with diameters ${\leq}10{\mu}m$ was the highest in February at Anmyon, Cheongwon and Ulleung, which is located leeward about half-way through the Korean Peninsula, AOD rose to a high in May. The growth of hygroscopic aerosols moving with increases in relative humidity prior to the Asian monsoon season contributed to a high AOD level in May. AE typically reaches its highest value ($1.30{\pm}0.37$) in August due to anthropogenic aerosols originating from industrial areas in Eastern China, while AOD stays low in summer due to the removal process caused by rainfall. The linear correlation coefficients of the MODIS AOD and ground-based mass concentrations of $PM_{10}$ at Anmyon, Cheongwon and Ulleung were 0.4-0.6. Four cases (six days) of mineral dustfall from sandstorms and six cases (twelve days) of anthropogenically polluted particles were observed in the central area of the Korean Peninsula in 2009. $PM_{10}$ mass concentrations increased at both Anmyon and Cheongwon in the cases of mineral dustfall and anthropogenically polluted particles. Cases of dustfall from sandstorms and anthropogenic polluted particles, with increasing $PM_{10}$ mass concentrations, exhibited higher AOD values in the Yellow Sea region.

Physical Properties of Reticulated Polyurethane Foams and the Enhancement of Microbial Adhesion through their Surface Treatments (망상 폴리우레탄 폼의 물성 및 표면처리를 통한 미생물 고정화 특성의 향상)

  • 김시욱;장영미;명성운;최호석
    • KSBB Journal
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    • v.18 no.5
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    • pp.412-417
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    • 2003
  • We first investigated basic characteristics of reticulated polyurethane (PU) foams as microbial carriers. In general, the specific surface area of PU foams increases with respect to decreasing pore sizes. However, the number of microbes adhered on the unit surface of reticulated PU foams decreases with respect to decreasing pore sizes. Thus, as a result of totally considering all effects such as apparent density, hydrolysis rate, and adhesion, we can know that PU foams with 45 PPI is the most appropriate microbial carrier. In this study, we can also investigate the effect of various physico-chemical surface treatments on the adhesion of microbes on the surface of PU foams. We used a chitosan treatment, a PEI (Polyethylene Imine) treatment, a xanthane treatment and a plasma treatment. As a result of comparing all surface treatments, the plasma surface treatment was the best.