• Korean Journal of Veterinary Research
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• v.36 no.4
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• pp.903-913
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• 1996

총 15두의 초유를 섭취하지 않은 신생자견을 대상으로 난황항체를 경구투여한 후 개 파보바이러스를 경구 접종하여 실험감염을 유발시켜 난황항체의 수동 면역에 의한 예방효과를 알아보고자 한다. 항체역가는 면역화된 산란계로부터 분리한 난황항체를 투여한 자견이 비면역 난황항체를 투여한 자견에 비해 높았다. 개 파보바이러스 접종 직전의 항체역가는 대조군의 경우 1:40에서 1:80, 실험군의 경우는 1:320에서 1:1280이었다. 모든 대조군의 자견들은 바이러스 접종후 4일에 임상증상을 나타내었고 총 7두중 6두가 폐사된 반면 실험군 자견은 2두만이 증상을 나타내었고 폐사 자견은 없었다(p<0.01). 개 파보바이러스를 경구 접종한 후 전체 자견의 혈구응집억제반응역가는 접종후 6일까지 감소하는 경향을 보였다. 접종후 5일의 분변내 혈구 응집반응역가는 실험군 자견의 경우 < 2에서 64였으며 대조자견은 216에서 2048로 높았다.

• Proceedings of the Korean Society of Veterinary Service Conference
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• 1994.04a
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• pp.134.3-135
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• 1994

• Korean Journal of Veterinary Research
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• v.36 no.4
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• pp.895-902
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• 1996

산란계에 불활화 개 파보바이러스 백신을 근육내로 1주 간격으로 4회 접종하여 면역화시키고 최종 접종 2주후에 채란하여 $4^{\circ}C$에 보관하며 사용하였다. 난황항체는 5% HPMCP를 이용하여 분리하였고 0.5% HPMCP 용액은 lipid 침전에 매우 효과적이었으며 희석배수 10배에서 투명한 상층액을 나타내었다. 1차분리한 상층액의 단백질 농도는 2.5mg/ml이었고 최종 단백질 용액의 경우는 26.53mg/ml이었다. SDS-PAGE 전기영동상에서 분자량 60~70 KD 및 30~40 KD의 2 band가 나타났으며 non-reducing 전기영동에서는 닭 혈청 IgG와 같은 120~160 KD의 분자량을 보인 band가 각각의 분리용액에서 나타났다. 난황 항체의 개 파보바이러스에 대한 혈구응집억제반응 항체역가는 혈청의 역가에 비해 1주의 차이를 주며 증가했으며 난황 항체는 1:640에서 1:2560, 혈청은 1:640에서 1:5120을 나타내었다.

• Journal of Veterinary Clinics
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• v.21 no.1
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• pp.1-6
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• 2004

This study was undertaken to provide the appropriate vaccination protocol of canine parvovirus (CPV) vaccine for the companion dogs in Korea. A total of 120 healthy pups (20 pups per group) at 6 weeks of age were randomly assigned to one of four commercially available vaccines [C, G, K, and V groups] and one of vaccination schedules [V2 and V4 groups]. The serological responses to the CPV component of the vaccines were determined by measuring HI titers. The maternal antibodies was declined to under the protective level at 6 weeks of age. Therefore, it was considered that vaccination of pups for CPV should be started at 6 weeks of age. And when the combination vaccine was used, the immunogenicity of V vaccine was superior to the other vaccines and optimum vaccination schedule was 3 times vaccination with 3 weeks-interval starting vaccination at 6 weeks of age. Although pups were vaccinated at 6 weeks of age, the geometric mean CDV titers of pups in all groups by 9 weeks of age were under the protective level. So, hygienic measures including avoiding to exposure to the high risk areas were needed to prevent CPV infection in this period.

• Journal of the korean veterinary medical association
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• v.17 no.2
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• pp.15-20
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• 1981

• Journal of the korean veterinary medical association
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• v.32 no.7
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• pp.472-476
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• 1996

• Proceedings of the Korean Society of Veterinary Service Conference
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• 1996.05a
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• pp.69.1-69
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• 1996

• Journal of the korean veterinary medical association
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• v.32 no.12
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• pp.767-775
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• 1996

• Korean Journal of Clinical Laboratory Science
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• v.55 no.1
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• pp.45-51
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• 2023

Canine parvovirus type 2 (CPV-2) and canine coronavirus (CCoV) are major pathogens that can induce gastroenteritis in dogs. They are highly contagious and have a high morbidity rate. There are no specific treatments available for them to date. Therefore, rapid and accurate diagnosis becomes essential. The rapid diagnostic test (RDT) for animals can be used widely in the field because it is fast and easy to use for diagnosis. Thus, this study aimed to clinically evaluate and confirm the clinical utility of CPV-2/CCoV RDT. The parameters evaluated included the limit of detection (LoD), cross-reactivity, interference, sensitivity, specificity, negative likelihood ratio (NLR), and kappa value. The results revealed that the LoD values for CPV-2 and CCoV were 9.7×10 50% tissue culture infectious dose (TCID₅₀)/mL and 2.5×10² TCID₅₀/mL, respectively. There was no cross-reactivity with nine pathogens or interference by interfering materials. The RDT showed a sensitivity of 90.0%, a specificity of 100.0%, NLR of 0.1, and a kappa value of 0.90 for diagnosing both viruses. In conclusion, CPV-2/CCoV RDT is useful as a screening test because of its high sensitivity, specificity, kappa value, and low NLR.

• Journal of Veterinary Clinics
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• v.22 no.4
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• pp.348-352
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• 2005

We described a rapid, sensitive and reproducible real-time PCR assay for detection and quantitation of canine parvovirus type 2 in the feces of dogs with parvovirus infection. The method was demonstrated to be highly specific and sensitive, allowing a precise canine parvovirus type-2 quantitation over range of eight orders of magnitude from $10^2\;to\;10^9$ copies of standard DNA. Then, fecal samples from parvovirus infected dogs were analyzed by conventional PCR and real-time PCR. Real-time PCR is more sensitive than conventional PCR, allowing to detect low viral titers of CPV-2 in infected dogs. By real-time PCR, a wide range of parvovirus particles was found in the samples from $1.45\times10^6\;to\;9.45\times10^8$ copies/0.01g of feces. However, when dogs are in infection of parvovirus, it is difficult to prove that the numbers of peripheral blood leukocytes are correlated with those of fecal shedding virus.