• Korean journal of applied entomology
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• v.49 no.4
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• pp.299-304
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• 2010

The striped fruit fly, Baetrocera scutellata (Diptera: Tephritidae), is one of the most important pests in Cucurbitaceae plants. Among 6 attractants for B. scutellata used, Cue-lure was shown better effect than the others. Newly emerged adult of B. scutellata had been occurred early June of the year tested. There were two peaks of occurrences of B. scutellata, one was from mid-July to early August and the other was early September suggesting that B. scutellata has two generation a year. The larva of B. seutellata damaging the female flower and stem of the pumpkin was found for the frist time on Korea. The blossoms were damaged and fallen by B. scutellata which were 53.8% of male flower and 30.7% of female flower in the 6 pumpkins. The pumpkin flowers were damaged 51.9% by B. scutellata. Especially thc pumpkin flowers damaged were shown 72.7% (1015/1397 individuals) from August to September. The larva of B. scutellata was found from the Trichosanthes kirilowii on 24 June 2009 in Jinan-gun Jeollabuk-do.

• Korean Journal of Environment and Ecology
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• v.25 no.5
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• pp.658-665
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• 2011

An wild boar species which has been known as an extinct species on Jeju Island, was recently observed in the surrounding areas of Mount Halla. Based on the molecular techniques, this study examines whether they are crossbred with domesticated pig breeds. Intraspecific genetic relationships with other wild boar populations and molecular sexing were examined as well. Total of four molecular markers on mitochondrial DNA(control region and ND2) and nuclear DNA(MC1R and KIT) were applied to test crossbreeding between with domesticated pig breeds, such as Landrace, Large White, Berkshire, Hampshire, and Duroc. All individuals of wild boar population had identical mtDNA control region(CR) sequences. In addition, the sequences were the same as those of some native pig breeds which are distributed in Northeast China, but different from those previously reported from the Korean Peninsula up to date. These results suggest that this population may have originated from a genetic lineage had been not previously studied and genetically related to Chinese native pig breeds. Molecular sexing results show that there are twice as many females as male. Thus the population is under expansion and its size will dynamically increase if not controlled.

• Korean Journal of Plant Taxonomy
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• v.39 no.4
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• pp.277-291
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• 2009

The flora in the region of Songnisan National Park ($N36^{\circ}28^{\prime}05^{{\prime}{\prime}}{\sim}36^{\circ}46^{\prime}38^{{\prime}{\prime}}$, $E\;127^{\circ}47^{\prime}06^{{\prime}{\prime}}{\sim}127^{\circ}57^{\prime}50^{{\prime}{\prime}}$) was surveyed from April 2000 to July 2006. Vascular plants in 26 collections included a total of 723 taxa: 115 families, 392 genera, 629 species, 4 subspecies, 72 varieties and 18 forms. Ninety-three of these taxa were first records for the region. In the flora of Songnisan National Park, 27 taxa were Korean endemic plants, 19 taxa were rare and endangered plants as designated by the Korea Forest Service. Forty-five of the taxa were with the levels III, IV, V in the floristic regional indicator plants which was designated by Korean Ministry of Environment, and 29 taxa were alien plants now naturalized in Korea. Owing to their unusual phytogeography and low number of populations, the taxa Crypsinus hastatus (Thunb.) Copel., Paeonia japonica (Makino) Miyabe & Takeda, Jeffersonia dubia (Maxim.) Benth. & Hook.f. ex Baker & S.Moore, Bupleurum euphorbioides Nakai, Phacellanthus tubiflorus Siebold & Zucc., Lilium cernuum Kom., Gastrodia elata Blume and Cephalanthera erecta var. subaphylla (Miyabe & Kud ) Ohwi were considered the most important. Based on these results, it is clear that appropriate plans to conserve the natural habitat of Songnisan National Park must be made immediately.

• Journal of Life Science
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• v.17 no.11
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• pp.1505-1510
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• 2007

We found 8 single nucleotide polymorphisms (SNPs) in adipocyte fatty acid bonding protein (FABP4) gene as candidate gene of FAT1 locus on pig chromosome 4. With over 800 heads of major commercial pig breeds including Duroc, Landrace, Berkshire and Yorkshire, we analyzed SNPs of FABP4 gene to determine possible effects of FABP4 genotype to economically important traits. $400{\sim}800\;bp$ amplicons in FABP4 gene were used PCR-RFLP for each SNPs and we found that the frequency of some SNPs of this gene was different among the breeds. According to the statistical analyses to determine possible associations of each genotype with economic traits, it was found that subgroup with different genotypes showed significant differences in daily gain, backfat thickness, lean percentage and feed conversion ratio (P<0.05). Thus, as a Part of enhancing the selection competence related to swine growth rate and lean percentage, it is expected that FABP4 gene markers verified in this study will be useful to use for Korean commercial pig industry.

• Journal of Life Science
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• v.21 no.9
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• pp.1329-1335
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• 2011

Korean native goats, which are characterized by black coat color, have existed on the Korean peninsula for a long time. Until now, there has been no comprehensive investigation concerning their genetic diversity, phylogenetic analysis or origin. In this study, we investigated the genetic diversity and verified phylogenetic status of the Korean native goat using the 453-bp fragment of the hypervariable fragment I (HVI) of mitochondrial DNA (mtDNA) D-loop region from 60 individuals among 5 populations. The Korean native goat showed less haplotype diversity when compared with goats from other countries. In addition, 6 haplotypes that had not been previously reported were verified in this study. In phylogenetic analyses with other country's goats, 10 haplotypes from Korean native goats were classified into mtDNA lineage A. Moreover, in a phylogenetic tree for goats which contained mtDNA lineage A, 8 of 10 haplotypes could be included in a subgroup with goats from Vietnam and an area of China. However, none of the remaining haplotypes belonged to a major group of Korean native goats and were located on different independent positions. These results suggest that almost Korean native goats aligned more closely to China and Vietnam breeds in mtDNA lineage A and there was no gene flow from other mtDNA lineages. Our results will contribute to conservation strategies and genetic breeding of Korean native goats.

• Korean Journal of Medicinal Crop Science
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• v.2 no.1
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• pp.81-85
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• 1994

This experiment was carried out to investigate the fertilization and the size of mature female and male gametophytic parts of Pulsatilla koreana after artificial pollination. The size of pollen is $26.5{\mu}m$ at the time of anther dehiscence, that is, about $3{\sim}4$ days after pollination. Synergid nucleus, egg nucleus, and polar nucleus are 10.0, 15.0, and $32.5{\mu}m$ respectively at the time of completing egg apparatus formation, that is, about 2 days after pollination. Poller tubes germinate on stigma about 10 hours, passing lower part of style about 30 hours, penetrating into micropyle about 35 hours after pollination. Sperm nucleus penetrates into polar nucleus about 40 hours and egg cell about 48 hours after pollination. But, there seems to be different among the individuals. Multinuclei and multinucleoli are formed in egg cell, synergid, and polar nucleus about the time of fertilization. Proembryo is formed about 4 days, being changed to large globular form about $6{\sim}8$ days after pollination. Endosperm nuclei divide into free nuclei after fertilization and change to cotyledon in gymnosperm. There seems to be same phenomena in Pulsatilla koreana.

• Journal of the Korean Institute of Landscape Architecture
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• v.45 no.6
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• pp.1-9
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• 2017

This study investigates the environmental factors of endemic species in Korea in order to understand their ecological characteristics, and to investigate the target species of their natural habitats to find similar sites. The purpose is to restore and follow suitable growth methods for the appropriate highway facility of target species to establish a management system via monitoring. This study endeavors to restore the target species near highway facilities on the basis of monitoring data and restore sites have similar natural characteristics of the target species. After restoring the target species, a restoration strategy and management plan will be established for breeding and continuation. The restoration strategy and management plan of the target species is divided into breeding, restoring, maintaining and monitoring plans. Specially management plans include several divisions such as soil, water, non-point pollution source reduction and naturalized plants. The results of this study can be used as reference materials for the restoration of endemic Korean plants in the future of highway routes, and for systematic management measures in habitats.

• Weed & Turfgrass Science
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• v.2 no.2
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• pp.191-197
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• 2013

Two medium-leaf ecotypes (CY6069, CY6097) belonging to one species (Zoysia japonica) of Korean lawngrasses were selected in sod production fields in Jang Seong, Korea. They were reported to have distinct morphological and growth rate characteristics different from the preferred medium-leaf type zoysiagrass in Korea. This study was conducted to define further the genotypic difference at the molecular level and to develop DNA marker based on the specific DNA fragment. Polymorphic DNA fragments were first explored by using randomly amplified polymorphic DNA (RAPD) primers, which were then converted into PCR-based sequence characterized amplified region (SCAR) markers. The CY6069-specific primer set amplified about 550 bp successfully, while the CY6097 marker produced the expected 690 bp band, by which those markers were nominated by CY6069_550 and CY6069_690 SCARs, respectively. Together with the reported morphological and other phenotypic features, the SCAR markers confirmed in this study will be useful to identify those medium-leaf zoysiagrass genotypes when they are cultivated with other vegetatively propagated warm-season turfgrasses in sod farms.

• Journal of Life Science
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• v.17 no.5 s.85
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• pp.613-618
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• 2007

Azoospermia factor(AZFa, b, and c) regions have been focused on their involvement in the spermatogenic process by frequent observation of microdeletion in male infertility. Among the azoospermia factors, RBMY1, CDY1, and VCY2 genes are strongly associated with the male germinal cell differentiation and development in testis. Using RT-PCR approach, expression patterns of RBMY1, CDY1, and VCY2 genes are examined in testicular biopsy specimens from 42 Korean azoospermic patients. No expression of RBMY1, CDY1, and VCY2 genes appeared as 34%, 66%, and 27% of the male infertility, respectively. Patients who had no expression of RBMY1 and VCY2 genes also showed negative expression of the CDY1 gene in their testis tissues. All Sertoli cell-only syndrome patients showed no expression of the CDY1 gene. Taken together, the CDY1 gene expression seems to be necessary factor to complete spermatogenesis in Korean population.

• Proceedings of the Korea Water Resources Association Conference
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• 2018.05a
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• pp.483-483
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• 2018

우리나라 하천 생태계에서 외래어종은 토착 어류의 개체수를 빠르게 감소시키고 서식처에서 우점종이 되어 생태계 교란을 야기하고 있다. 이러한 현안의 문제점을 해결하기 위해 외래어종과 관련된 많은 연구가 진행되고 있다. 본 연구는 우리나라 대표적 외래어종인 배스와 블루길을 대상으로 수온에 따른 서식처 형성을 관찰한다. 그 후 계절별로 형성된 서식처 안에서 수온을 조절하여 외래어종의 생태환경에 따른 민감성을 분석하는 것을 목적으로 수행하였다. 실험실 연구를 진행하는 동안 외래어종이 서식처를 형성할 때 민감하게 반응하는 수온 차가 계절에 따라 달라짐을 발견하였다. 이 사실을 증명하기 위해 농업용 저수지로부터 채집한 배스와 블루길을 길이 12 m, 깊이 0.6 m, 폭 1 m의 하천과 유사한 자연적 환경이 갖추어진 수조에 이식한 후, 외래어종이 서식처를 형성할 때 계절에 따라 어떻게 반응하는지 면밀히 관찰하였다. 수조를 3개의 구역으로 나누어 좌측 끝단을 히터를 설치한 온수대, 우측 끝단을 냉각기를 설치한 냉수대, 중앙은 온도 조절장치를 설치하지 않은 중수대로 구분하여 실험하였고 계절 마다 수온을 조절하며 수온 차와 서식처 형성의 상관성 분석이 진행되었다. 실험 진행 시 수온을 제외한 수조 안 환경은 동일하게 유지하였다. 실험 결과, 동계에는 실험실 수조의 평균 수온이 $12.88^{\circ}C$이고 어류는 각 구간별 수온의 차이가 $1^{\circ}C$ 이하에도 민감하게 반응하며 조금이라도 수온이 높은 구역에 서식처를 형성하였다. 춘계에는 평균 수온 $16.15^{\circ}C$가 되면서 어류가 온수대에 서식처를 형성하기는 하나 각 구간별 $1^{\circ}C$ 정도의 차이에는 중수대까지 서식처를 형성하였다. 즉 동계보다는 수온 차이에 둔감하게 반응하는 특성을 보였다. 또한 춘계에서 하계로 넘어가는 과도기에 수조의 평균 수온이 $21^{\circ}C$로 올라가자 외래어종은 각 구간별 수온 차이와는 관계없이 이미 서식처로 형성했던 온수대를 넘어 수조 전체에 좀 더 확산된 형태의 서식처를 형성하였다. 하계에는 수조의 평균 수온이 $25.67^{\circ}C$였으며 수온을 조절하여 관찰한 결과 외래어종은 약 $24^{\circ}C$ 수온보다 낮은 냉수대에 서식처를 뚜렷하게 형성하는 모습을 보였다. 즉 외래어종은 $20{\sim}24^{\circ}C$ 정도의 수온에 서식처를 가장 선호 하며 동계에는 수온이 가장 높은 곳, 하계에는 가장 낮은 곳에 형성하는 것으로 판단된다. 실험을 진행하는 동안 수조 내 구역의 구분을 위해 공통으로 설치된 가림판 부근에 외래어종의 군락이 형성된 것을 통해, 향후에는 외래어종의 서식처 형성과 장애물과 같은 지형적인 요인이 미치는 영향에 관한 연구가 필요할 것으로 판단된다.