• The Journal of Society for e-Business Studies
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• v.13 no.3
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• pp.121-135
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• 2008

Railroad logistics transporting freight by train takes charge of 10 or 20 percent of domestic cargos. Railroad logistics users such as transport companies including shippers or Inland Container Depot(ICD) use electronic document(EDI or XML) or input data through WEB to process railroad logistics business. However, as business environments evolving into e-business, it is required to upgrade the legacy railroad logistics process. As the increase of using ebXML-based schema format, it is also needed to improve the electronic documents based on DTD format into those of XML schema format. This study deals with information standard for railroad logistics to improve the railroad logistics business. To this purpose railroad business processes were re-defined through the standard business process modeling methodology. Information model was also derived by defining railroad logistics activities from business process model. And Business Information Entities(BIEs) were defined to design new electronic documents according to the extracted information model. An improved system architecture for railroad logistics was proposed as well. The results of this study will provide an effective and flexible business flow to railroad logistics business.

• Weed & Turfgrass Science
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• v.3 no.4
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• pp.305-311
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• 2014

The cultivation area and use of genetically modified (GM) crops have been increased continuously over the world. Seed distribution and transgenes to environmental ecosystem is one of the most important factors in risk assessment and risk management of GM crop. Safe management for the development and commercialization of GM crops is required according to The Act on Transboundary Movements of Living Modified Organisms,etc (LMO Act) in Korea. This study was conducted to setup the environmental monitoring system of GM zoysiagrass (event JG21 and JG21-MS). The monitoring was performed in 4 GMO confined fields, Sungwhan, Ochang, Jeju University and Jeju Namwon. In the result of monitoring, we could not found any gene flow and distribution of GM zoysiagrass in the 3 fields, but one spill of JG21 was found in the Namwon field in 2012. These results suggest that continuous monitoring is necessary to detect the occurrence of GM zoysiagrass for preventing genetic contamination in natural environment.

• Journal of the Korean Association of Geographic Information Studies
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• v.20 no.3
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• pp.181-194
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• 2017

This study aims to develop a regression model for forest volume estimation using field-collected forest inventory information and airborne LiDAR data. The response variable of the model is forest stem volume, was measured by random sampling from each individual plot of the 30 circular sample plots collected in Bonghwa-gun, Gyeong sangbuk-do, while the predictor variables for the model are Height Percentiles(HP) and Height Bin(HB), which are metrics extracted from raw LiDAR data. In order to find the most appropriate model, the candidate models are constructed from simple linear regression, quadratic polynomial regression and multiple regression analysis and the cross-validation tests were conducted for verification purposes. As a result, $R^2$ of the multiple regression models of $HB_{5-10}$, $HB_{15-20}$, $HB_{20-25}$, and $HBgt_{25}$ among the estimated models was the highest at 0.509, and the PRESS statistic of the simple linear regression model of $HP_{25}$ was the lowest at 122.352. $HB_{5-10}$, $HB_{15-20}$, $HB_{20-25}$, and $HBgt_{25}-based$ models, thus, are comparatively considered more appropriate for Korean forests with complicated vertical structures.

• Journal of Plant Biotechnology
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• v.44 no.1
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• pp.89-96
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• 2017

The CRISPR/Cas9 is a core technology that can result in a paradigm for breeding new varieties. This study describes in detail the sgRNA design, vector construction, and the development of a transgenic plant and its molecular analysis, and demonstrates how gene editing technology through the CRISPR/Cas9 system can be applied easily and accurately. CRISPR/Cas9 facilitates targeted gene editing through RNA-guided DNA cleavage, followed by cellular DNA repair mechanisms that introduce sequence changes at the site of cleavage. It also allows the generation of heritable-targeted gene mutations and corrections. Here, we present detailed procedures involved in the CRISPR/Cas9 system to acquire faster, easier and more cost-efficient gene edited transgenic rice. The protocol described here establishes the strategies and steps for the selection of targets, design of sgRNA, vector construction, and analysis of the transgenic lines. The same principles can be used to customize the versatile CRISPR/Cas9 system, for application to other plant species.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2017.04a
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• pp.165-165
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• 2017

모돈은 사육 특성상 제한된 파일롯 공간 안에 장시간 머물기 때문에 과중한 몸무게에 의한 지제 이상, 섭식 등의 불량, 수면상태의 불량 등을 지속적으로 관찰해야 하는 대상이다. 측면에 다수의 초음파 센서를 설치하여 기립의 상태 및 운동 시 몸체 궤적의 특성을 분석하여 종합적으로 모돈의 행동 특성을 정량화 하고자 하였다. 이 과정에서 계측 신호의 값을 대수적으로 비교하는 방식에 한계가 있음을 발견하였고, 이를 해결하고자 10 Hz/Ch 내외의 시계열 상대거리 궤적 신호를 주파수 도메인으로 변경하여 분석을 수행하였다. 일정 주파수에 집중되어 있는 주파수 값의 크기 변화(파워 스펙트럼 밀도)를 기준으로 모돈의 움직임의 정상 상태 유무 판별이 가능하였다. 단, 이러한 분석은 계측 데이터를 일괄 처리 방식으로 분석하는 방법으로 도출이 되었으므로, 계측과 정량 분석을 동시에 수행하기 위한 개선이 필요하였다. 계측 시스템에서 사용한 마이크로 프로세서는 Nucleo-446(STMelectronics, CA, USA)로 180 Mhz의 클럭 속도로 작동하나, 총 100 Hz 내외의 16비트 계측 신호에 대해 추가적으로 FFT 등의 주파수 변환 신호 처리를 수행하기에는 연산 능력이 부족하였다. 한편, 주파수 분석의 주기를 1분 단위로 할 경우 처리해야할 정보의 크기는 $100{\times}60{\times}5{\times}2Byte$ 이므로 1분 내에 해당 연산을 종료할 수 있는 추가의 연산 장치가 필요하였다. 계측과 주파수 도메인 변환 연산을 동시에 수행하기 위하여 1 Ghz의 연산능력을 가진 ARM A9 계열의 초소형 멀티코어 AP인 NanoPi Neo Air(Friendlyarm, Guangzhou, China)을 선정하였다. 4개의 코어를 각각 계측, Median 필터링, Smoothing 연산, FFT 분석에 사용하여 1분 단위, 2분 단위, 5분 단위의 주파수 분석을 동시에 수행하였다. 병렬 연산 라이브러리는 오픈 소스인 MPICH(www.mpich.org)를 이용하였다. 상대적으로 여유있는 자원을 보유하고 코어를 실시간으로 결정하여 다수의 모돈 개체 동시 모니터링을 위한 네트워크 연결 역할을 동시에 수행하도록 하였다. 1주일 내외의 요인 실험 수행 결과, 약 70 Mbyte의 데이터가 축적이 되었으며, 1분 단위, 2분 단위, 5분 단위의 주파수 도메인 변환 후 결과를 동시에 취득할 수 있었다. 일부 주파수 도메인 상의 파워 밀도 값이 모돈의 행동 특성에 분석에 유효한 정보를 제공함을 발견하였다. 모돈사 내 현장 보급이 가능한 초소형 AP와 멀티 코어 기반 병렬 처리 기법을 이용한 현장 진단 시스템 개발 연구를 지속적으로 수행할 것이다.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2017.04a
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• pp.164-164
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• 2017

스마트 돈사 환경의 복지 및 생산성 향상을 위하여 정량 분석법을 기반으로 한 모돈 관리의 중요성이 증가하고 있다. 모돈은 교배, 임신, 분만, 포유, 이유를 순환적 반복하여 이루어지는데 모돈의 관리는 돈사 농장의 생산성 및 경제성과 직결된다. 모돈 관리에 필요한 환경 및 계측정보를 획득하고 이 정보로부터 모돈의 개체관리를 극대화시키고 최적의 방안을 찾고자 지속적으로 계측이 가능한 모돈의 돈사 모니터링 시스템이 필요하다. 모돈의 행동특성 계측이 가능한 시스템이 필요한 이유는 모돈의 행동 특성(섭식 및 지제불량 등)에 상응하는 대사 불량, 질병 및 발정 징후 등을 조기에 발견할 수 있기 때문이다. 돈사 내에서 정지 상태로 판별이 되는 모돈의 지제상태(기립상태, 누운 상태, 앉은 상태)와 다르게 연속적인 움직임으로부터 판별되는 모돈의 섭식상태를 분석하기 위해서는 계측 시스템과 이를 분석해주는 시스템간의 시간적 차이를 최소화 할 수 있는 실시간 신호 처리 기술이 필수적이다. 모돈의 섭식을 정량적으로 지수화하기 위한 센서의 최소 SPS(sample per second)는 600 Hz($100Hz{\times}6$개)로서 최소 6개 ADC 채널과 최소 1,200 Hz 이상으로 샘플링 할 수 있는 마이크로 컨트롤러가 필요하다. 또한 16 비트의 분해능으로 1분 동안 연속 계측을 수행할 경우 필요한 정보량은 153,600 KByte ($1,200sample/s{\times}16bit/sample{\times}8Byte/bit$)으로 실시간 처리를 수행하기에 매우 큰 정보량이라 판단할 수 있다. 수행하고자 하는 정보처리 기법에 따라 다소 상이할 수 있으나, 1분을 주기로 모돈의 섭식 분석을 수행하고자 할 경우 최도 150 MByte의 정보량을 처리하기 위한 최소의 클럭수는 단순 대입의 경우 2.5 Mhz (clock/second) ($=1clock/Byte{\times}150MByte/60seconds$) 이며 덧셈(4 clock)의 경우 10 Mhz, 곱셈(16 clock)의 경우 40 Mhz의 클럭이 필요하다. 또한 정보의 저장 및 도시를 위해 필요한 부가적인 회로(LCD, SD메모리) 구동을 위해 필요한 클럭을 고려할 경우 추가적인 클럭이 필요하다. 이를 종합적으로 고려하여 120 Mhz ($= 40Mhz{\times}3$) 이상의 클럭이 필요하다고 판단할 수 있다. 또한 센서 계측 주기의 시간 분해능을 균등하게 유지하기 위해선 계측->도시->저장의 과정을 교차적으로 수행해야 한다. 이러한 과정을 거처 최종적으로 선정한 마이크로 프로세서는 ARM Cortex-M4이며 168 MHz로 연산 수행이 가능하여 목표하고자 하는 신호처리를 수행 할 수 있다. 현장 예비 실험을 통해 기대 성능을 만족하였으며, 시간 복잡도가 높은 연산을 대비하여 최적 시분할 스케쥴링 기법에 대한 보완이 필요하다고 판단되었다.

• Journal of Korean Society of Forest Science
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• v.105 no.4
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• pp.422-428
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• 2016

Ginkgo (Ginkgo biloba L.) is one of the most appropriate roadside trees because of a good transplantation nature and ability to grow well in urban environment. Ginkgo is a dioecious species. Sex discrimination of ginkgo is possible through comparing morphological characters of reproductive organs. However, it needs more than about twenty years for reproductive organs to appear after sexual maturity. Until now, ginkgo trees for roadside plantation have been planted without discriminating the sex because ginkgo trees have been usually planted before sexual maturity. Ginkgo nuts from the female ginkgo trees planted along the roadside emit a foul odor, and make much pollution on the streets. Thus in this study a novel SCAR marker (SCAR-GBM) for the early sex discrimination was developed. Primers were developed on the basis of the sequence of male-specific RAPD variants reported previously. False-negative problem of SCAR marker, probably caused by dominant nature, was resolved by using multiplex PCR using primers of both the SCAR-GBM and a universal primer set of atp1 region in mitochondria DNA, which resulted in improved discrimination efficiency. The results showed that DNA bands of 1,039 bp were commonly amplified by the atp1 primer set in male and female trees, and SCAR-GBM markers of 675 bp were specifically amplified only in male trees. Reproducible and specific discrimination of the multiplex PCR was finally confirmed by applying multiple male and female individuals.

• Journal of the Korean Society for Library and Information Science
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• v.46 no.2
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• pp.261-280
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• 2012

This study seeks to replace PaRIS Principles and find directions of revising KCR4 by analyzing the international Cataloging Principles 2009(ICP 2009) established in the environment of Machine Readable Cataloging. ICP 2009 was reflected in cataloging rules such as RDA and ISBD 2010 as the minimal principles for uniformity in establishing each nation's cataloging rules. In contrary, KCR4 needs to be revised because it has never been changed after 2003, and has only description rules without any rules for the choice and forms of access points. Therefore, this study aims to grasp requirements that should be reflected in KCR4 through analyzing ICP 2009. In first step, it is to grasp the features of ICP 2009 by comparing PaRIS Principle and ICP 2009 and to compare KCR4 in aspects of ICP 2009. The detailed elements for comparison between ICP 2009 and KCR4 are scope, general principles, entitles, attributes, and relationships, objectives and functions of the catalogue, bibliographic description, access points, foundations for search capabilities as the contents of ICP 2009. As a result, this study could give some directions of KCR4 in the future. First, ISBD 2010 and conceptual models should be reflected in KCR4 in description. Second, it should regulate the authority access points in KCR4 based on ICP 2009. Third, it will describe essential access points of work and expression attributes in bibliographic records and authority records to find works and expression. This study will contribute to guide the national cataloging rules.

• Korean Journal of Animal Reproduction
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• v.25 no.1
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• pp.9-17
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• 2001

To determine the effect of the characteristics of the corpus luteum (CL) and serum metabolites on pregnancy rate following embryo transfer in Hanwoo cow, recipients were synchronized the estrus with CIDR. in vivo or in vitro produced bovine embryos were transferred to synchronized recipients. The characteristics of the CL were checked by rectal palpation and ultrasound. Blood samples were collected from jugular vein at Day 7 and analysed progesterone and serum metabolites. The results obtained from this study were as follows; 1. Total 397 embryos were transferred to recipient and 121 heads out of them were pregnant. The pregnancy rate was 30.5% and slightly differences between farms. 2. There was not significantly different the pregnancy rates between the size of CL and the cavity CL. In the recipients which had more than 2ng/$m\ell$ of serum progesterone level, the pregnancy rates was in higher than others (46.6% vs. 24.4%, 15.0%). 3. The highest pregnancy rate was obtained at 90 to 110mg/㎗ in serum total cholesterol, 14 to 16mg/㎗ in BUN, and 70 to 80mg/㎗ in serum glucose, respectively, and these metabolites in blood could be used as the criteria in the selection of recipient at embryo transfer.

• Korean Journal of Remote Sensing
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• v.33 no.6_2
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• pp.1139-1149
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• 2017

Measuring tree's volume is very important input data of various environmental analysis modeling However, It's difficult to use economical and equipment to measure a fragmented small green space in the city. In addition, Trees are sensitive to seasons, so we need new and easier equipment and quantification methods for measuring trees than lidar for high frequency monitoring. In particular, the tree's size in a city affect management costs, ecosystem services, safety, and so need to be managed and informed on the individual tree-based. In this study, we aim to acquire image data with UAV(Unmanned Aerial Vehicle), which can be operated at low cost and frequently, and quickly and easily quantify a single tree using SfM-MVS(Structure from Motion-Multi View Stereo), and we evaluate the impact of reducing number of images on the point density of point clouds generated from SfM-MVS and the quantification of single trees. Also, We used the Watertight model to estimate the volume of a single tree and to shape it into a 3D structure and compare it with the quantification results of 3 different type of 3D models. The results of the analysis show that UAV, SfM-MVS and solid model can quantify and shape a single tree with low cost and high time resolution easily. This study is only for a single tree, Therefore, in order to apply it to a larger scale, it is necessary to follow up research to develop it, such as convergence with various spatial information data, improvement of quantification technique and flight plan for enlarging green space.