• Microbiology and Biotechnology Letters
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• v.39 no.2
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• pp.111-118
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• 2011

For the strain improvement of the industrial polyploid yeast strain through hybridization and protoplast fusion, a dominant selection marker other than a recessive marker such as the auxotrophic marker was required for the selection of the resulting hybrids. In the present investigation, the aureobasidin A resistant gene was tested in relation to whether it can be used as the dominant selectable marker for the isolation of hybrids of the yeast Saccharomyces. The plasmid pAUR112, carrying the gene responsible for resistance to aureobasidin A, was introduced into the haploid yeast strain K114/YIp. From the rare-mating between polyploid C6 and haploid K114/YIp carrying pAUR112, many hybrids were obtained from the agar medium containing 0.5 ${\mu}g$/ml of aureobasidin A. The hybrids exhibited characteristics derived from both of the parental strains; and the cell sizes of the hybrids were larger than those of the parental strains. These results showed that the aureobasidin A resistant gene could be successfully used as the dominant selectable marker for the isolation of yeast hybrids resulting from rare-mating.

• Microbiology and Biotechnology Letters
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• v.36 no.2
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• pp.158-164
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• 2008

The fusants which contain starch utilizing ability and alcohol fermenting ability were developed by protoplast fusion of Saccharomyces cerevisiae KOY-1 and Saccharomyces diastaticus KCTC 1804. Sacharomyces cerevisiae KH-12 was obtained by haploid induction from Saccharomyces cerevisiae KOY-1. The auxotropic mutants of yeast were obtained by using an ethylmethane sulfonate (EMS). The frequency of protoplast formation in Saccharomyces cerevisiae KOY-1 $(Met^-)$ and Saccharomyces diastaticus KCTC 1804 $(Trp^-)$ were 90.5% and 97.7%, respectively. The frequency of fusant formation was $1.79{\times}10^{-4 }$ for the regenerated protoplast and the 1,000 fusants were obtained. Fusant FA 776 was selected as a potential yeast which contain an alcohol fermenting ability in the starch medium. The genetic stability was 4.64% for 10 passages of generation. Fusant FA 776 produced 13mg/ml of alcohol in 24% starch medium and showed 1.86-fold higher alcohol fermenting ability than Saccharomyces diastaticus KCTC 1804.

• Journal of the Korean Society of Hazard Mitigation
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• v.3 no.1 s.8
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• pp.149-155
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• 2003

The reason of source for a tragic death by the occurrence of an event is $\ulcorner$unobservant of safety rule,$\lrcorner$ $\ulcorner$negligence of staff,$\lrcorner$ $\ulcorner$deficiency of responsibility$\lrcorner$ and $\ulcorner$deficiency of safety value$\lrcorner$ and for certain reason that is insufficiency of space for safety education also think little of a people, unobservant of safety rule, selfishness and so on. It makes new and specific plan that is simulation room before I compare the inside and outside of the country for a nation the reform of consciousness. Next, go ahead with a plan for about safety-simulation room. The Government can support to plan if it make a proposal to center of Government. It should be make a people of safety system, and it build the safety-simulation room $1{\sim}2$ in the city or county. And, the city cannot build the big safety-simulation room the reason are size of building and money. Accordingly, it will be make a system and installation for different type of safety-simulation room for about calamity, disaster and different type of accident in Korea. It must be build the building of space that should be make a space of knowledge and information by many books and media, develop of education program from life-safety.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.04a
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• pp.141-141
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• 2003

신선농산물의 호홉속도를 측정하는 방법 중 하나인 개방계(open system) 호흡속도 측정시스템은 소정의 농도로 조정된 혼합기체를 측정대상시료에 흘려 보내며 측정하는 방법이다. 개방계 측정법의 장점은 혼합 기체조성 영역에서 정확한 호흡속도를 얻을 수 있으며 방치시간이 필요 없으므로 반복 측정이 용이한 것 등이다. 그러나 개방계 측정법은 공급되는 혼합기체의 농도와 유속이 일정하여야 하며 연속으로 호흡속도 측정용 챔버의 혼합기체 공급측과 배기측에서 기체시료를 수집하여 매우 미세한 기체농도의 차이를 측정할 수 있어야 하고 기체 시료 수집에 상당한 주의가 요구된다. 이러한 문제를 개선하기 위하여 개방계 호흡속도 측정 시스템을 자동화하였다. 자동화된 호흡속도 측정 시스템은 혼합기체 발생장치, 온도조절이 가능한 기체기밀용 챔버와 G.C로 구성되어 있다. 환경기체조성을 위한 혼합기체발생장치는 $N_2$, $O_2$, $CO_2$ 압축 실린더에서 공급되는 기체를 압력 조절기를 통해서 일차압력을 조정하고 정밀 압력 조절기를 이용하여 0.1~0.2 kg/$\textrm{cm}^2$의 정압을 유지시켰다. 압력이 일정해진 기체는 metering valve를 이용하여 각 기체의 유량을 소정의 비율로 제어할 수 있도록 하였으며 각각의 기체는 gas mixed cell에서 실험 농도의 환경기체조성으로 혼합되어 항온기내의 호흡속도 측정 챔버($25^{\circ}C$)로 공급될 수 있도록 하였다. 호흡속도 측정용 챔버는 개스킷이 장착된 아크릴 재질이며 온도 조절이 가능한 항온기로 구성되어 있다. 호흡속도 측정용 챔버와 G.C간의 기체흐름은 three way solenoid valve에 의하여 제어되며 전원의 on/off에 따라 공급측의 가스와 배기측의 가스가 선택적으로 G.C에 공급될 수 있도록 구성하였다. 측정 대상 챔버의 기체는 제어된 유로를 따라 multi-position valve를 통과하여 G.C에서 분석되도록 하였다. 본 연구에서 개발된 개방계 호흡속도 자동 측정 시스템의 성능 실험에서 혼합기체발생장치에서 조제된 혼합 기체의 농도를 설정치와 비교한 결과 $O_2$와 $CO_2$의 농도에서 평균오차 0.2%로 정밀한 것으로 나타났으며 호흡속도 측정용 챔버의 혼합기체 공급측과 배기측의 가스 농도를 3회 반복 측정한 결과 재현성에서는 0.1%이하의 편차로 나타났다. 개방계 호흡속도 자동 측정 시스템을 이용하여 환경기체조성하에서 토마토의 호흡속도를 측정하는 실측 실험을 수행한 결과 2$0^{\circ}C$에서 12.7~42.1mg$CO_2$/kg.hr였으며 12$^{\circ}C$에서 2.5~8.2mg$CO_2$/kg.hr로 일반적으로 보고되고 있는 토마토 호흡속도와 일치하는 결과를 나타내었다.

• Food Science and Preservation
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• v.12 no.5
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• pp.496-500
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• 2005

To develop functional food material using Rhodiola dumulosa(RD), the biological activities such as antioxidation, antiproliferation in the cancer cells and immuno-activity in macrophage cells were investigated with hexane, ethylacetate, n-butanol, methanol and water fractions of RD 80% methanol extract. Hydrogen-donating activities of hexane, ethyl acetate, n-butanol, methanol and water fraction were 28.30, 53.21, 35.48, 42.64 and 21.14%, respectively, at a concentration of 100 ${\mu}g/mL$, and the activity of ethyl acetate fraction was similar to as that of BHT. After treated for 48 hrs, the ethyl acetate fraction decreased the proliferation of the A549 and SW480 cells in a dose-dependent manner at concentration of 10, 50, and 100 ${\mu}g/mL$, the activities were higher than other fractions. Morphology of cells treated with the ethyl acetate fraction for 48 hr at 100 ${\mu}g/mL$ was distorted with shrank cell mass, and the cell number was lower than that of control cells the macrophage cells treated. The methanol fraction was significantly induced NO production compared with untreated control cells at above 10 ${\mu}g/mL$ concentration. These results indicate that RD would be used the functional food material.

• Journal of the Korean Society of Marine Environment & Safety
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• v.25 no.6
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• pp.678-686
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• 2019

In this study, marine heat waves on coastal waters of Republic of Korea were detected using satellite-based Sea Surface Temperature Anomaly (SSTA). The detected results were compared with the warm water issues reported by the National Institute of Fisheries Science (NIFS). Marine heat waves detection algorithm using SSTA based on a threshold has proposed. The threshold value was defined as 2℃ for caution and 3℃ for warning issues, respectively. Daily averaged SST data from July to September of 2017-2018 were used to generate SSTA. The satellite-based detection results were classified into nine areas according to the place names used in the NIFS warm water issues. In the comparison of frequency of marine heat waves occurrence to each area with the warm water issue, most areas in the southern coast showed a similar pattern, that is probably NIFS uses spatially well distributed buoys. On the other hand, other sea areas had about two times more satellite detection results. This result seems to be because NIFS only considers the water temperature data measured at limited points. The results of this study are expected to contribute to the development of a satellite-based warm/cold water monitoring system in coastal waters.

• Korean journal of communication and information
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• v.81
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• pp.9-39
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• 2017

Online communities have become playgrounds for enjoyment and political public spheres as well as have been vitalized by participation of diverse users, which shows that users of online communities experience participatory democracy and new subjects who project the politic of everyday life eventually. The purpose of the study is to examine that how phenomenon from above have been revealed in the academic discourses and seek the ways for development of the theory and methodology of community media research. To do this, I analyze that features and tendencies of academic discourses which have historically been accumulated through applying method of meta-analysis into topics, methods, and issues in the precedence studies. Data is centered on Journals of the Korean Society for Journalism and communication Studies, Korean Association for Broadcasting and Telecommunication studies, Korean Association for Communication and Information studies, and the Cybercommunication Academic Society. The reason why I choose these journals is that they have a long history of the publication compared to other journals, thus, I can collect various related articles. Total of 53 Samples are selected after input keywords '공동체' and 'Community'. This study makes a contribution by offering the preliminary data in order to seek the ways for revitalization of local communities and for the future theories and methodologies of community media through researching on precedence studies having piled from 2001 when the first article about community media was published to November 2016.

• Journal of Korean Home Economics Education Association
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• v.28 no.1
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• pp.1-17
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• 2016

The purpose of this review was to introduce and examine 'development' and 'relations' as the core concepts of Home Economics in 2015 revised middle school curriculum in Korea. The 2009 and 2015 proclamation of the ministry of education on home economics curriculum and 26 published middle school textbooks were reviewed. The major findings were as follows. First, the components of human 'development' and family 'relations' were strongly associated with promoting four key competencies (i.e. the self-management competency, the communication competency, the aesthetic sensibility competency, the community competency) of 2015 revision. Also, four of cross-curricular learning topics (i.e. character education, multicultural education, safety and health education, human right education) in 2015 revision could be effectively discussed with human 'development' and family 'relations'. Second, when teaching and learning of the core concept, human 'development', continuous dynamic aspects of life-span development, the connectedness of different domains of development, systematic approach of various concepts in development, specificity and empirical evidence of information and variability of developmental patterns in adolescence should be considered. Third, when teaching and learning of the core concept, family 'relations', family trait such as generational relations, gender relations, role relations and power relations should be taken into account. In addition, exclusively focusing on normal family ideology or image of middle-class family and lecture-centered instruction methods should be changed for students to achieve the competencies relevant to family relations. The future directions for applying core concepts, 'development' and 'relations' in classroom will be discussed.

• Journal of Korean Home Economics Education Association
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• v.22 no.2
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• pp.81-99
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• 2010

This study was motivated by the fact that the educational curriculum needs revising as the contemporary society undergoes dramatic changes; this change demonstrates that Korea society now turns into a multicultural community. In this vein, this study aims to examine how the national Home economics curriculum (revised in 2007) incorporated the essence of multicultural education program and that of international understanding program into the coursebook, and to suggest which part of the coursebook needs revising to enhance its validity. To attain this goal, this research was conducted to analyze how two programs are implemented in secondary school, and to propose how those programs should be developed to address the immediate needs of the current society. To illustrate, this study examines a variety of theories and methodology on how multicultural education program should be implemented along with the current situation of Korea society. In addition, quantitative research was conducted to acquire empirical data from 6 coursebooks the frequencies of statements and visual aids that are considered to be directly reflect the essence of two programs. The results demonstrate that two programs are relegated into a lesser position compared to other programs; furthermore, they lack materials to deepen students' understanding about multicultural society and international relations. Therefore, the considerable revision needs to be conducted to embrace the essence of multicultural society and international understanding programs, and sensitize students to the immediate needs of the current society.

• Korean Journal of Childcare and Education
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• v.12 no.1
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• pp.143-170
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• 2016

This paper organized a cooperative activity using character-related picture books for guiding children to cooperate as a part of an effort to present solutions against the increasing tendency of individualism and the de-communization phenomenon among serious social issues affecting children in modern society. This paper aims to help the children to build the proper caring thinking for others and understanding the self by applying those cooperative activities. The subjects were 50 children of 4 years old in K and S day care centers in city C. The data were analyzed by caring thinking tool using t-test. It also recognized change to children's caring thinking of play situation by way of video and observation journal. The results of the research found that the experimental group's caring thinking score was significantly higher than that of the control group. In accordance with the general analysis of the program results, it was verified that the cooperative activity's use of character-related picture books included caring thinking, understanding the self, and respecting others' emotionsin them. The results of this study can be used as basic data to develop an educational activity program for character development and help with the application of character-related picture books.